• Microbiology and Biotechnology Letters
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• v.18 no.1
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• pp.89-93
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• 1990

To improve the stability of recombinant pBR322-trip$^+$ plasmid (pLTW24) in E. coli culture, a positive selection system was devised. A DNA fragment containing pheW$^+$ gene (a structural gene for tRNA$^{phe}$ was isolated and inserted into the pBR322-trip$^+$ plasmid(pLTP24). A temperature sensitive host strain. LC901-pheS$^{-ts}$, was constructed for this plasmid by transducing pheS$^{-ts}$ allele (phenylalanyl-tRNA synthetase) to E. coli LC901 using P1kc bacteriophage. The LC901-pheS$^{-ts}$ cells were unable to grow at a restrictive temperature when they had lost the pBR322 :: pheW$^+$ (pLTP24) plasmid. The effects of pheW$^+$ gene on the plasmid stability and the expression level of trip$^+$ gene in LC901-pheS$^{-ts}$ strain were investigated. The proportion of Trip$^+$ colonies among LC901-pheS$^{-ts}$ strain carrying plasmid pLTP24 was 99%, whereas that of LC901 strain carrying plasmid pLTW24 was 7% at the end of 20 generations. After 100 generations of growth, the strain LC901-pheS$^{-ts}$ carrying plasmid pLTP24 showed little loss of plasmids. While the majority of plasmid pLTW24 in LC901 strain were lost in the same period. The activities of tryptophan synthetase (T. Sase) and anthranilate synthetase (A. Sase) in LC901 strain carrying pLTW24 were about 1.2 times and 1.8 times respectively of those in LC901-pheS$^{-ts}$ strain carrying pLTP24.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.24 no.3
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• pp.179-188
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• 2012

Performance analyses of vertical breakwaters were conducted for fictitiously designed breakwaters for various water depths to analyze the influence of climate change on the structures. The performance-based design method considering sea level rise and wave height increase due to climate change was used for the performance analysis. One of the problems of the performance-based design method is the large calculation time of wave transformation. To overcome this problem, the SWAN model combined with artificial neural network was used. The significant wave height and principal wave direction at the breakwater site are quickly calculated by using a trained neural network with inputs of deepwater significant wave height and principal wave direction, and tidal level. In general, structural stability becomes low due to climate change impacts, but the trend of stability is different depending on water depth. Outside surf zone, the influence of wave height increase becomes more significant, while that of sea level rise becomes negligible, as water depth increases. Inside surf zone, the influence of both wave height increase and sea level rise diminishes as water depth decreases, but the influence of wave height increase is greater than that of sea level rise. Reinforcement and maintenance policies for vertical breakwaters should be established with consideration of these results.

• Proceedings of the KWS Conference
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• 2009.11a
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• pp.108-108
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• 2009

Precipitation hardening nickel base alloys strengthened by intermetallic compounds are extensively used to manufacture on the components of the hot section of gas turbine engines. To ensure structural stability and maintenance of strength properties for a long time, nickel alloys are normally subjected to complex alloying with elements to form ${\gamma}'$(gamma prime). Such alloys have a limited weldability, are normally welded in high temperature. However, laser welding have a merit that applies in room temperature as easy control of welding parameter and heat input. In this study, $CO_2$ laser welding is applied on STS304 plate with good ductility and precipitation hardening nickel base alloy (GTD111DS) used blade material. Also, several welding parameters are applied on powder, power and travel speed. There are no cracks in Rene 80 and IN 625 powder when STS304 plate is used. But IN 625 powder has no cracks and Rene 80 have some cracks in welds with GTD111DS substrate. Adjusting of welding parameter is tried to apply Rene 80 having a good strength compare to IN 625. In the result of adjusted welding parameter, optimized welding parameters are set with low power, low feed rate and high welding speed. Tensile strength of GTD111DS substrate with Rene 80 powder is same and over than the one of base metal in room temp and high temp($760^{\circ}C$).

• Journal of Life Science
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• v.26 no.12
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• pp.1392-1399
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• 2016

Jaspine B is an anhydrophytosphingosine that is isolated from a marine sponge. Because of its structural similarity to sphingosine, it shows anti-cancer effects in human carcinomas. Therefore, this study aims to investigate its anti-proliferative effect on various cancer cells and to correlate its association with the intracellular accumulation of Jaspine B in relevant cancer cells. The anti-proliferative effect of Jaspine B in various cancer cells was determined by a cell viability test, and the intracellular concentration of Jaspine B in relevant cancer cells was determined using mass spectrometry coupled with liquid chromatography. The correlation coefficient and p value between the cytotoxicity and the cell accumulation of Jaspine B were determined using SPSS 16.1. The cytotoxicity of Jaspine B varied depending on the type of cancer cell when compared the $EC_{50}$ values of Jaspine B. Breast and melanoma cancer cells were susceptible to Jaspine B, whereas renal carcinoma cells were resistant. The intracellular concentrations of Jaspine B had a reciprocal correlation with the $EC_{50}$ values in the same cells (r = 0.838). The results suggested that the anti-proliferative effect of Jaspine B was associated with the cellular accumulation of this compound. However, Jaspine B was not a substrate for P-glycoprotein and breast cancer resistance protein, as major efflux pumps caused multidrug resistance. The maintenance of a high intracellular concentration is crucial for the cytotoxic effect of Jaspine B; however, efflux pumps may not be a controlling factor for Jaspine B-related resistance in cancer cells.

• Fire Science and Engineering
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• v.29 no.6
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• pp.51-56
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• 2015

In Korea, measures to maintain sustainable fire safety performance for apartment buildings are insufficient in terms of fire-fighting products, skilled personnel, and maintenance status. Also, because of the particular features of a fire compartment, it has structural problems that are very likely to cause damage to human life when a fire occurs. Currently, problems with the fire supervisory system installed in an apartment building cannot be checked in real time, so it is difficult to identify the location of a fire accurately. Protected areas are also not assigned to each household, and residents cannot be clearly informed of the occurrence of a fire. As a consequence, safety evacuation cannot be secured. In addition, it is impossible to test the operation performance for water detectors in sprinkler fire extinguishing systems outside of the household. Therefore, an experiment was conducted to evaluate the performance of a remote fire supervisory system. The results show that the system satisfies all performance requirements. Also, an household alarm system was installed in each household to alert of any occurrence of a fire accurately, and the performance of the alarm system was improved to ensure that residents were quickly evacuated.

• International Journal of Highway Engineering
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• v.8 no.3 s.29
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• pp.49-61
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• 2006

This study suggests long-life asphalt pavement method which can save maintenance cost by increasing the design and performance period of pavements. The high modulus asphalt binder developed and then various physical tests are performed. Laboratory performance tests and accelerated pavement test are conducted for the high modulus and conventional mixtures. The test results show that dynamic modulus values of high modulus mixtures are higher than those of the conventional mixtures, The high modulus mixtures yield better fatigue, rutting and moisture damage performance than conventional mixtures. Structural analysis is performed and a database is built up for long life asphalt pavement design. Pavement response model is developed through a multiple regression analysis program, SPSS using the database. A design software for the long life pavements is developed based on the pavement response model and laboratory and field performance tests results. In addition, optimum pavement sections and materials are suggested. The suggested AC thickness of long life asphalt pavement is 29cm. A Life cycle cost analysis(LCCA) is conducted to check the economical efficiency of the long life pavement section. The LCCA result shows that initial construction costs of long life and conventional pavements are almost equal, but long life pavement is more profitable in terms of the LCCA.

• International Journal of Highway Engineering
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• v.7 no.3 s.25
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• pp.1-10
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• 2005

In this study, accelerated pavement tester(APT) was performed on long-life asphalt pavements that can save maintenance and user costs by increasing the design life twice longer than conventional asphalt pavements. Basic material testings are first conducted on a high modulus base(HMB) mixture developed in this study. Four different pavement sections including thin and thick conventional and thin and thick HMB courses are constructed to compare the load-carrying capacities and to investigate the fatigue and rutting performances using an accelerated pavement tester. Tensile strain values at the bottom of base courses under the various loading levels are measured. The tensile strain values of the HMB sections are lower than those of the conventional sections. It is observed from the APT performed on the thin pavement sections that no significant cracks are developed up to the 180,000 cycles of a wheel load. In terms of rutting, only 3mm of rutting is developed in the thick HMB section while 5.3mm of rutting is developed in the thick conventional section at the 90,000 cycles of the wheel load. The HMB material developed in this study can be successfully used in the long-life asphalt pavements because of its excellent fatigue and rutting performances. It is estimated from a series of structural analysis that the use of the HMB material instead of the conventional base materials may reduce the asphalt thickness at least 5cm because of its better load-carrying capacity.

• International Journal of Highway Engineering
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• v.12 no.2
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• pp.9-16
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• 2010

Roughness is the most important factor to maintain the road performance, and affects greatly on the design life in Jointed Plain Concrete pavements. Also, the factors the evaluate pavement‘s commonality is the three method such as functionality, safety and structural performance. In evaluating function of road, representative factors is the roughness, which has been used to determine maintenance time as key standard. As research for roughness is absence in pavement design. Applied roughness-model had a low-reliability in Korea. Therefore, it is needed to develop reliable model in road roughness. In this research, uniform specific is applied to distribute them after selecting the concrete pavements. Concrete pavement is divided by sections of 238. Total length of this sections has 281km and account for 16% of total road length in korean concrete pavements for selected sections. Considering the korean roughness-model, the evaluation of roughness is performed for the freezing index, average annual rainfall, condition for the base, the amount of traffic as well as spalling(%), cracking(%), age(year) at the selected section at the selected section. Also, additional sections is selected to evaluate various age which affects on the roughness. As a result of the analysis, it showed that spalling(%), cracking(%), age(year), and the condition of the base affected road roughness. When the correlation with the road roughness was analyzed, the reliable model for road roughness was proposed, and the ratio that can explain road roughness was R2-68.8% and P value-0 which is statistically meaningful.

• Journal of Korea Water Resources Association
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• v.40 no.7
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• pp.503-510
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• 2007

In the area of such a nature-friendly stream improvement, it is not established yet which engineering method is suitable for stream environment, due to lack of technology. Therefore, although nature-friendly stream improvement was done with expensive engineering method, the effect has not been fully confirmed, which results from the absence of overall valuation tool of stream improvement. In this regard, it is necessary to develop and apply comprehensive and diverse valuation methods covering stream functions to the analysis of stream improvement. In this study, we collected data from years' of monitoring on the Gyeongcheon river, which is located in Sunchang-eup, Jeollabuk-do and recently underwent an nature-friendly stream improvement work. Based on the data, we developed a series of valuation methods such as stream naturalness evaluation, life cycle evaluation, amenity evaluation, and economic benefit analysis to consider the environmental function of stream from a comprehensive perspective. Stream naturalness evaluation is a quantitative analysis of how natural a stream is, and includes additional valuation items such as ecosystem and water quality for the purpose of overall valuation, unlike existing research focusing on physical elements and structural characteristics of a stream. We developed a method of stream valuation with life cycle assessment to river reorganization project. Amenity evaluation method was developed as a means to analyze residents' satisfaction with stream improvement through questionnaires. Economic benefit analysis was developed as a means to determine the attributes of environmental water supply, ecosystem, river maintenance, and water quality and predict economic benefits using contingent valuation method (CVM) and multi-attribute utility analysis (MAUA) method in order to analyze economic benefits brought in by stream improvement. It is considered that the four methods developed in this study make possible to conduct an overall and quantitative analysis of stream improvement.

• Economic and Environmental Geology
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• v.40 no.5
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• pp.661-673
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• 2007

Rock materials of the three-storied stone pagoda in the Cheongryongsa temple in Korea are mainly composed of gneissose two-mica granite and fine-grained granite. This stone pagoda shows structural instability due to cracks and breaking-out of the stones. The surface properties of the stone is highly degraded by various inorganic pollutants and epilithic biospecies. Therefore, this study carried out comprehensive deterioration diagnosis by non-destructive methods, and some conservation treatments base on the diagnosis were carried out to reduce weathering progress. As the treatments, the biospecies and lichen that covering on the stone surfaces were removed by dry and wet cleaning, and degraded concrete applied to the pagoda for restoration in the past was removed and repaired with epoxy resin. Oxidized iron plates inserted between the rock properties were also substituted titanium stainless steels. After all processes are completed, we sprayed consolidant on the rock surface. Finally, the ground of the stone pagoda was rearranged using small rock aggregates, and the fence was established for control of artificial deterioration by visitors and environmental maintenance.