• Journal of Microbiology and Biotechnology
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• v.8 no.2
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• pp.158-164
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• 1998

Crystal structural analyses of the API-TLCK complex revealed that the ${\epsilon}$-amino group (NZ) of the lysyl part of TLCK forms hydrogen bonds with OD1 of $Asp^225$ which is a substrate specificity determinant of API, OG of $Ser^214$, O of $Ser^214$, OG1 of $Thr^189$, and O of $Thr^189$ l89/. The ${\beta}$-carboxyl oxygen of $Asp^225$ forms hydrogen bonds with the NE1 of $Trp^182$. From these observations, it is thought that besides $Asp^225$, $Thr^189$, $Ser^214$, and $Trp^182$ may also contribute to the steric specificity for lysine and high proteolytic activity of API. The side-chain hydroxyl groups of $Thr^189$ and $Ser^214$ were removed to elucidate the role of these hydrogen bonds in the $S_1$-pocket. The $k_{cat}$/$K_m$ of T189V, S214A, and T189V.S214A were decreased to 1/4, 1/3, and 1/46, respectively, of the value for native API. The decreased activities were mainly due to the increase of $K_m$. The CD and fluoroscence spectra of the three mutants were similar to those of wild-type API. With regards to the kinetic parameters ($K_i\;and\;k_2$) of mutants for the reaction involving TLCK and DFP, $k_2$decreased by increase of $K_1$ only. These results suggest that the decreased catalytic activity of these mutants is caused by the partial loss of the hydrogen bond network in the $S_1$-pocket. On the other hand, the similarity of enzymatic properties between W182F and the native enzyme suggests that the hydrogen bond between OD2 of $Asp^225$ and NE1 of $Trp^182$ is not directly related to the reaction of $Asp^225$ with the substrate.

• Earthquakes and Structures
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• v.14 no.4
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• pp.323-336
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• 2018

Machine foundations with impact loads are common powerful sources of industrial vibrations. These foundations are generally transferring vertical dynamic loads to the soil and generate ground vibrations which may harmfully affect the surrounding structures or buildings. Dynamic effects range from severe trouble of working conditions for some sensitive instruments or devices to visible structural damage. This work includes an experimental study on the behavior of dry dense sand under the action of a single impulsive load. The objective of this research is to predict the dry sand response under impact loads. Emphasis will be made on attenuation of waves induced by impact loads through the soil. The research also includes studying the effect of footing embedment, and footing area on the soil behavior and its dynamic response. Different falling masses from different heights were conducted using the falling weight deflectometer (FWD) to provide the single pulse energy. The responses of different soils were evaluated at different locations (vertically below the impact plate and horizontally away from it). These responses include; displacements, velocities, and accelerations that are developed due to the impact acting at top and different depths within the soil using the falling weight deflectometer (FWD) and accelerometers (ARH-500A Waterproof, and Low capacity Acceleration Transducer) that are embedded in the soil in addition to soil pressure gauges. It was concluded that increasing the footing embedment depth results in increase in the amplitude of the force-time history by about 10-30% due to increase in the degree of confinement. This is accompanied by a decrease in the displacement response of the soil by about 40-50% due to increase in the overburden pressure when the embedment depth increased which leads to increasing the stiffness of sandy soil. There is also increase in the natural frequency of the soil-foundation system by about 20-45%. For surface foundation, the foundation is free to oscillate in vertical, horizontal and rocking modes. But, when embedding a footing, the surrounding soil restricts oscillation due to confinement which leads to increasing the natural frequency. Moreover, the soil density increases with depth because of compaction, which makes the soil behave as a solid medium. Increasing the footing embedment depth results in an increase in the damping ratio by about 50-150% due to the increase of soil density as D/B increases, hence the soil tends to behave as a solid medium which activates both viscous and strain damping.

• Journal of Conservation Science
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• v.36 no.2
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• pp.82-92
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• 2020

Modern artworks are constructed using a variety of materials and techniques. Sculptures, which predominantly consist of metals, usually have different shapes and consist of different material mixtures. The structural and material properties of these sculptures are often controlled by conservation treatment methods. However, the application of existing treatment methods is challenging at times, indicating that more diverse treatment materials and techniques are necessary. Therefore, in this study, a treatment method that employs volatile corrosion inhibitor(VCI) powder, rather than an anti-corrosion solution, for the conservation and management of metal artworks was used. VCI powder and VCI films containing VCI powder were used, and the results obtained confirmed that both of them showed anti-corrosion effect. Only a slight change in the chromaticity of metal samples was observed, and compared to the untreated samples, the application of the VCI powder resulted in a decrease in the rate of corrosion by half. Moreover, VCI film tests revealed that comparing to the untreated or polyethylene film-treated samples, VCI film treatment resulted in a decrease in the occurrence of corrosion compounds. The contact angle, surface energy, and surface electrical resistance were measured, and the evaluation of these surface properties established the anti-corrosion effect of VCI. Additionally, direct application of VCI and VCI films on actual sculptures further confirmed the anti-corrosion effect of VCI.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.2
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• pp.697-703
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• 2017

This paper examines the flexure behavior of FRP-concrete composite structure that can replace conventional reinforced concrete structure types. In order to investigate the structural performance and behavioral characteristics in numerical analysis means, ABAQUS, a general purpose finite element analysis program, was utilized for nonlinear finite element analysis, and the various variables and their influences were analyzed and compared with experimental results to suggest values optimized to this composite structure. The concrete damage plasticity model and Euro code for concrete were used. In the implicit finite element analysis, the convergence was ambiguous when geometrical and material nonlinearity were large, so the explicit finite element analysis used in this study was deemed to be appropriate. From the comparison with the experiment about concrete damaged plasticity model, 20mm for the mesh size, $30^{\circ}$ for the dilation angle, $100Nmm/mm^2$ for the value of fracture energy, 0.667 for Kc value, and the consideration of damage parameter were suggested believed to be appropriate. The numerical model suggested in this study was able to imitate the ultimate load and cracking pattern very well; therefore, it is expected to be utilized in research of various new material composite structures.

• Journal of the Korean Society of Marine Environment & Safety
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• v.26 no.5
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• pp.542-550
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• 2020

The use of high-strength aluminum alloys for ships and of shore structures has many benefits compared to carbon steels. Recently, high-strength aluminum alloys have been widely used in onshore and of shore industries, and they are widely used for the side shell structures of special-purpose ships. Their use in box girders of bridge structures and in the topside of fixed platforms is also becoming more widespread. Use of aluminum material can reduce fuel consumption by reducing the weight of the composite material through a weight composition ratio of 1/3 compared to carbon steel. The characteristics of the stress strain relationship of an aluminum structure are quite different from those of a steel structure, because of the influence of the welding[process heat affected zone (HAZ). The HAZ of aluminum is much wider than that of steel owing to its higher heat conductivity. In this study, by considering the HAZ generated by metal insert gas (MIG) welding, the buckling and final strength characteristics of an aluminum reinforcing plate against longitudinal compression loads were analyzed. MIG welding reduces both the buckling and ultimate strength, and the energy dissipation rate after initial yielding is high in the range of the HAZ being 15 mm, and then the difference is small when HAZ being 25 mm or more. Therefore, it is important to review and analyze the influence of the HAZ to estimate the structural behavior of the stiffened plate to which the aluminum alloy material is applied.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.4
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• pp.335-356
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• 1997

Injection of bovine growth hormone (bGH) to lactating dairy cows increases milk yield and yields of milk components including fat. It is generally believed that most of the anabolic effects derived from bGH in animal tissues are primarily mediated by IGF-1. IGF-1 is a strong anabolic peptide in the plasma of animals and exerts mitogenic and metabolic effects on target cells. Contrary to most protein hormones, the majority of IGF-1 in circulation is bound to the binding proteins (IGFBPs) which are known to be responsible for modifying the biological actions of IGF-1, thus making determinations of IGF-1 actions more difficult. On the other hand, fat is a major milk component and the greatest energy source in milk. Currently, the fat content of milk is one of the major criteria used in determining milk prices. It has been known that flavor and texture of dairy products are mainly affected by milk fat and its composition. Acetyl-CoA carboxylase (ACC) is the rate limiting enzyme which catalyzes the conversion of acetyl-CoA to malonyl-CoA for fatty acid synthesis in 1ipogenic tissues of animals including bovine lactating mammary glands. In addition to the short-tenn hormonal regulation of ACC by changes in the catalytic efficiency per enzyme molecule brought about by phosphorylation and dephosphorylation of the enzyme, the long-term hormonal regulation of ACC by changes in the number of enzyme molecules plays an essential role in control of ACC and lipogenesis. Insulin, at supraphysiological concentrations, binds to IGF-1 receptors, thereby mimicking the biological effects of IGF-1. The receptors for insulin and IGF-1 share structural and functional homology. Furthermore, epidermal growth factor increased ACC activity in rat hepatocytes and adipocytes. Therefore, it can be assumed that IGF-1 mediating bGH action may increase milk fat production by stimulation ACC with phosphorylation (short term) and/or increasing amounts of the enzyme proteins (long term). Consequently, the main purpose of this paper is to give the readers not only the galactopoietic effects of bGH, but also the insight of bGH action with regard to stimulating milk fat synthesis from the whole body to the molecular levels.

• The Korean Journal of Quaternary Research
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• v.1 no.1
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• pp.3-20
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• 1987

With regard to the Quaternary formations in the Korean Peninsula, very few studies have been done specially from a stratigraphic viewpoint. The alluvial sediments filling in the valleys have often been considered as the only formation of Quaternary age (more precisely of the Holocone) and so the Pleistocene was regarded as an erosional or nondepositional episode. This is apparently evident from a quick look at the general geological maps of the Peninsula, which show a lithological sequence of Mesozoic or Paleozoic substrata immediately overlain by Holocene alluvium. Likewise, the Pleistocene period was described in terms of unconformity in most local or regional stratigraphical successions of the Peninsula. Recently several different types of Quaternary formation, besides the so-called Holocene alluvium in the valley plain have been found around the Peninsula. They consist of coastal deposits, marine or fluviatile terrace deposits, ancient valley fill deposits or slope deposits. Some parts of the volcanic sediments in Jeju Is. are also known as the Quaternary sequence. Thus the Quaternary deposits in the Peninsula are far more developed than previously known to gelolgists. Moreover the importance of Quaternary research became recently apparent in Korea due to the shortage of raw materials and to the policy of an optimum land-use. Advanced constructions and land reclamation have required more precise engineering parameters of loose materials and an estimation of land stability. This does not imply only the engineering, or the structural properties of the loose material, but at the same time the basic study of the sediments from the stratigraphical and environmental viewpoints has been necessary. In this connection, Quaternary outcrops specially along the coastal fringe of the Peninsula have been mapped, profiled and sampled for sedimentological, clay-mineralogical and palaeomagnetic studies. All these results are compiled for the core of the Quaternary stratigraphy of the Peninsula.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.3
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• pp.453-461
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• 2000

Aquatic humic substance (AHS) was extracted from Soyang lake water using XAD-8 resin. and its concentration distribution in the lake from May to October. 1997 was determined. Ozonation characteristics of the AHS were studied as factors of pH, carbonate ion concentration. and the biodegradability and structural changes of the AHS were also investigated after ozonaticn. DOC distribution in Soyang Lake water was 1~3 mg/L. and the concentrations of AHS ranged between 0.2~0.8 mg/L. which was corresponding to 20~30% of DOC. AHS was composed of around 20% of HA and 80% of FA. The optimum pH value for AHS ozonation was in range of pH 7~9. When carbonate ions were added for AHS ozonation as a ladical scavenger. it was found that DOC removals were decreased. and the absorbance decreases were increased slightly. Biodegradability of the ozonized AHS was 50% higher than that of unozonated AHS. $^{13}C-NMR$ analysis showed that the aromatic compounds of AHS, after ozonation, were decreased from 49% to 17%. and the aliphatic and carboxylic compounds were increased from 34% to 51% and from 17% to 32%. respectively.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2004.04a
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• pp.179-186
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• 2004

Introduction of wave model, considered the effect of shoaling, refraction, diffraction, partial reflection, bottom friction, breaking at the coastal waters of complex bathymetry, is a very important factor for most coastal engineering design and disaster prevention problems. As waves move from deeper waters to shallow coastal waters, the fundamental wave parameters will change and the wave energy is redistributed along wave crests due to the depth variation, the presence of islands, coastal protection structures, irregularities of the enclosing shore boundaries, and other geological features. Moreover, waves undergo severe change inside the surf zone where wave breaking occurs and in the regions where reflected waves from coastline and structural boundaries interact with the incident waves. Therefore, the application of mild-slope equation model in this field would help for understanding of wave transformation mechanism where many other models could not deal with up to now. The purpose of this study is to form a extended mild-slope equation wave model and make comparison and analysis on variation of harbor responses in the vicinities of Pohang Old Harbor and Pohang New Port, etc. due to construction of New Port in Youngil Bay. This type of trial might be a milestone for port development in macroscale, where the induced impact analysis in the existing port due to the developemnt could be easily neglected.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.301-310
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• 2010

Bacillus subtilis strains produce a broad spectrum of bioactive peptides. The lipopeptide surfactin belongs to one well-known class, which includes amphiphilic membrane-active biosurfactants and peptide antibiotics. Both the srfA promoter and the ComP-ComA signal transduction system are an important part of the factor that results in the production of surfactin. Bs-M49, obtained by means of low-energy ion implantation in wild-type Bs-916, produced significantly lower levels of surfactin, and had no obvious effects against R. solani. Occasionally, we found strain Bs-M49 decreased spore formation and the development of competence. Blast comparison of the sequences from Bs-916 and M49 indicate that there is no difference in the srfA operon promoter PsrfA, but there are differences in the coding sequence of the comA gene. These differences result in three missense mutations within the M49 ComA protein. RT-PCR analyses results showed that the expression levels of selected genes involved in competence and sporulation in both the wild-type Bs-916 and mutant M49 strains were significantly different. When we integrated the comA ORF into the chromosome of M49 at the amyE locus, M49 restored hemolytic activity and antifungal activity. Then, HPLC analyses results also showed the comA-complemented strain had a similar ability to produce surf actin with wild-type strain Bs-916. These data suggested that the mutation of three key amino acids in ComA greatly affected the biological activity of Bacillus subtilis. ComA protein 3D structure prediction and motif search prediction indicated that ComA has two obvious motifs common to response regulator proteins, which are the N-terminal response regulator receiver motif and the C-terminal helix-turn-helix motif. The three residues in the ComA N-terminal portion may be involved in phosphorylation activation mechanism. These structural prediction results implicate that three mutated residues in the ComA protein may play an important role in the formation of a salt-bridge to the phosphoryl group keeping active conformation to subsequent regulation of the expression of downstream genes.