• Journal of Microbiology
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• v.43 no.3
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• pp.285-294
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• 2005

A bacterial strain M4-7 capable of degrading various polyesters, such as poly$(\varepsilon-caprolactone)$, poly(3-hydroxybutyrate-co-3-hydroxyvalerate), poly(3-hydroxyoctanoate), and poly(3-hydroxy-5-phenylvalerate), was isolated from a marine environment and identified as Pseudomonas alcaligenes. The relative molecular mass of a purified extracellular medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA) depolymerase $(PhaZ_{palM4-7})$ from P. alcaligenes M4-7 was 28.0 kDa, as determined by SDS-PAGE. The $PhaZ_{palM4-7}$ was most active in 50 mM glycine-NaOH buffer (pH 9.0) at $35^{\circ}C$. It was insensitive to dithiothreitol, sodium azide, and iodoacetamide, but susceptible to p-hydroxymercuribenzoic acid, N-bromosuccinimide, acetic anhydride, EDTA, diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride, Tween 80, and Triton X-100. In this study, the genes encoding MCL-PHA depolymerase were cloned, sequenced, and characterized from a soil bacterium, P. alcaligenes LB19 (Kim et al., 2002, Biomacro-molecules 3, 291-296) as well as P. alcaligenes M4-7. The structural gene $(phaZ_{palLB19})$ of MCL-PHA depolymerase of P. alcaligenes LB19 consisted of an 837 bp open reading frame (ORF) encoding a protein of 278 amino acids with a deduced $M_r$ of 30,188 Da. However, the MCL-PHA depolymerase gene $(phaZ_{palM4-7})$ of P. alcaligenes M4-7 was composed of an 834 bp ORF encoding a protein of 277 amino acids with a deduced Mr of 30,323 Da. Amino acid sequence analyses showed that, in the two different polypeptides, a substrate-binding domain and a catalytic domain are located in the N-terminus and in the C-terminus, respectively. The $PhaZ_{palLB19}$ and the $PhaZ_{palM4-7}$ commonly share the lipase box, GISSG, in their catalytic domains, and utilize $^{111}Asn$ and $^{110}Ser$ residues, respectively, as oxyanions that play an important role in transition-state stabilization of hydrolytic reactions.

• The Journal of the Korea Contents Association
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• v.11 no.3
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• pp.351-367
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• 2011

Few previous researches have been studied in terms of franchisees' perceptions of franchisors' entrepreneurial activities within the boundary of restaurant franchising industry settings. The sampling frame for this study included 605 restaurant franchisees in the business-format franchising system in Korea and data were collected from 1 October 2009 to 28 February 2010. This study, from franchisees' views, investigated the relationship among franchisors' entrepreneurship, external environment, organizational structure(mechanistic-organic) and managerial performance, with structural equation modeling(SEM). The research findings were, firstly, external environment positively affected entrepreneurship which positively affected market orientation and managerial performance, and secondly, market orientation made franchisors select organic structure and heighten managerial performance. Also, external environment led franchisors to adopt organic structure and aggravated their managerial performance. The implications of these findings were as follows. Firstly, the more franchiors had initiatives, innovatedness and risk-taking attitudes, the higher they had managerial performances and market orientation. Secondly, franchisors should keep and heighten market share with keeping in mind with customer orientation, competition orientation, harmony between departments, long-term orientation and profitability. Thirdly, continuous and cautious environmental scanning was necessary because external restaurant environment made organizational structure being changed and managerial performance being aggravated.

• Applied Microscopy
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• v.33 no.1
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• pp.79-92
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• 2003

Ultrastructural changes of the pericarp in Citrus reticulata has been investigated during hesperidium abscission. The pericarp was composed of compactly arranged parenchyma cell layers during early stages of fruit development. The outermost exocarp was green and active in photosynthesis. However, cells in the exocarp soon changed into collenchyma cells by developing unevenly thickened walls within a short time frame. As the fruit approached maturation, the chlorophyll gradually disappeared and chloroplasts were transformed into carotenoid-rich chromoplasts. In the mature fruit the exocarp consisted of large, lobed collenchyma cells with primary pit fields and numerous plasmodesmata. The immature mesocarp was a relatively hard and thick layer, located directly under the exocarp. With development, the deeper layers of the exocarp merged into the white, spongy mesocarp. Before separation of the hesperidium from the plant, some unusual features were detected in the plasma membrane of the exocarp cells. The number of small vacuoles and dark, irregular osmiophilic lipid bodies also increased enormously in the exocarp collenchyma after the abscission. They occurred between the plasma membrane and the wall, and invaginated pockets of the plasma membrane containing double-membraned vesicles were also frequently noticed. The lipid bodies in the cytoplasm were often associated with other organelles, especially with plastids and mitochondria. The plastids, which were irregular or amoeboid in shape, contained numerous large lipid droplets, and occasional clusters of phytoferritin, as well as few loosely -oriented peripheral lamellae. Myelin-like configurations of membrane were frequently observed in the vacuoles, as was the association of lipid bodies with the vacuolar membrane. Most vacuoles had an irregular outline, and lipid bodies were often connected to the tonoplast of the vacuoles. The structural changes underlying developmental, particularly to senescence, processes in various hesperidium will be reported in the separate paper.

• The Journal of Korean Society of Virology
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• v.30 no.1
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• pp.71-81
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• 2000

We have determined and analyzed the full-length cDNA sequence of a coxsackievirus B3 (CVB3) Korean isolate (CVB3-Korea/97) which has been known as a general human pathogen. The whole genome contains 7,400 nucleotides and has a single large open reading frame with 6,555 nucleotides that encodes a potential polyprotein precursor of 2,185 amino acids. The genome also contains a 5' non-coding region (NCR) of 741 bases and a 3' NCR of 104 bases followed by poly(A) tail. Sequence homologies of nucleotides and deduced amino acids between the CVB3-Korea/97 strain and the prototype (Nancy strain) were 81.7% and 91.5%, respectively. The genes encoding the functional proteins including viral protease and RNA dependent RNA polymerase showed higher homology than those encoding the structural proteins. We have further analyzed the sequences of 5' NCR, VP1 and VP2 of CVB3-Korea/97, which are known as cardiovirulent determining factors at the nucleotide and amino acid levels. Although the CVB 3-Korea/97 strain was isolated from an aseptic meningitis patient without cardiomyopathy, its 234th nucleotide and 165th amino acid were uracil and Asn as same as those of other cardiovirulent strains one. However, the 155th amino acid of VP1, which closely associated with cardiovirulence, was replaced with $Arg^{155}$ by single nucleotide substitution from $A^{2916}$ to $T^{2916}$. Moreover, additional amino acid substitutions were observed in the flanking region of $Asp^{155}$. Taken together, amino acid(s) substitution in VP1 may playa critical role in determining cardiovirulence of the CVB3-Korea/97 strain rather than individual nucleotide replacements in the 5' NCR and/or an amino acid substitution in VP2.

• Proceedings of the Microbiological Society of Korea Conference
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• 2000.05a
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• pp.23-36
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• 2000

Japanese encephalitis virus (JEV) is the causative agent of a mosquito-borne encephalitis and is transmitted to human via persistently infected mosquito vectors. Although the virus is known to cause only acute infection, there were reports that showed neurological sequelae, latent infection in peripheral mononuclear cells, and recurrence of the disease after acute encephalitis. Innate resistance of certain cell lines, abnormal SN1 expression of the virus, and anti-apoptotic effect of cullular bcl-2 have been suggested as probable causes of JEV persistence even in the absence of defective interfering (DI) particles. Although possible involvement of DI particles in JEV persistence was suggested, neither has a direct evidence for DI presence nor its molecular characterization been made. Two questions asked in this study are whether the DI virus plays any role in JEV persistent infection if it is associated with and what type of change(s) can be made in persistently infected cells to avoid apoptosis even with the continuous virus replication, DI-free standard stock of JEV was infected in BHK-21, Vero, and SW13 cells and serial high multiplicity passages were performed in order to generate DI particles. There different-sized DI RNA species which were defective in both structural and nonstructural protein coding genes. Rescued ORFs of the DI genome maintained in-frame and the presence of replicative intermediate or replicative form RNA of the DI particles confirmed their replication competence. On the other hand, several clones with JEV persistent infection were established from the cells survived acute infections during the passages. Timing of the DI virus generation during the passages seemed coincide to the appearance of persistently infected cells. The DI RNAs were identified in most of persistently infected cells and were observed throughout the cell maintenance. One of the cloned cell line maintained the viral persistence without DI RNA coreplication. The cells with viral persistence released the reduced but continuous infectious JEV particle for up to 9 months and were refractory to homologous virus superinfection but not to heterologous challenges. Unlike the cells with acute infection these cells were devoid of characteristic DNA fragmentation and JEV-induced apoptosis with or without homologous superinfection. Therefore, the DI RNA generated during JEV undiluted serial passage on mammalian cells was shown to be biologically active and it seemed to be responsible, at least in part, for the establishment and maintenance of the JEV persistence in mammalian cells. Viral persistence without DI RNA coreplication, as in one of the cell clones, supports that JEV persistent infection could be maintained with or without the presence of DI particles. In addition, the fact that the cells with JEV persistence were resistant against homologous virus superinfection, but not against heterologous one, suggests that different viruses have their own and independent pathway for cytopathogenesis even if viral cytopathic effect could be converged to an apoptosis after all.

• Journal of the Korea Concrete Institute
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• v.29 no.1
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• pp.43-51
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• 2017

As per current seismic design codes, diagonally reinforced coupling beams are restricted to coupling beams having aspect ratio below 4. However, a grouped diagonally reinforcement detail makes distribution of steel bars in the beam much harder, furthermore it may result in poor construction quality. This paper describes the experimental results of concrete coupling beam reinforced with high-strength steel bars (SD500 & SD600 grades). In order to improve workability for fabricating coupling beams, a headed large diameter steel bar was used in this study. Two full-scale coupling beams were fabricated and tested with variables of reinforcement details and aspect ratio. To reflect real behavior characteristic of the beam coupling shear walls, a rigid steel frame system with linked joints was set on the reaction floor. As a test result, it was noted that cracking and yielding of reinforcement were initially progressed at the coupling beam-to-shear wall joint, and were progressed to the mid-span of the coupling beam, based on the steel strain and failure modes. It was found that the coupling beams have sufficient deformation capacity for drift ratio of shear wall corresponding to the design displacement in FEMA 450-1. In this study, the headed horizontal steel bar was also efficient for coupling beams to exhibit shear performance required by seismic design codes. For detailed design for coupling beam reinforced with high-strength steel, however, research about the effect of variable aspect ratios on the structural behavior of coupling beam is suggested.

• Journal of Korean Association for Spatial Structures
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• v.8 no.5
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• pp.47-58
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• 2008

Spatial structure is an appropriate shape that resists external force only with in-plane force by reducing the influence of bending moment, and it maximizes the effectiveness of structural system. With this character of the spatial structure, generally long span is used. As a result, large deflection is accompanied from the general frame. the structure is apt to result in a large deflection even though this structure experiences a small displacement in absence. Usually, nonlinear analysis in numerical analysis means geometric nonlinearity and material nonlinearity and complex nonlinearity analysis considers both of them. In this study, nonlinear equation of equilibrium considering geometric nonlinearity as per finite element method was applied and also considered the material nonlinearity using the relation of stress-strain in element. It is applied to find unstable result for tracing load-deflection curve in the numerical analysis tech. especially Arc-length method, and result of the analysis was studied by ABAQUS a general purpose of the finite element program. It is found that the present analysis predicts accurate nonlinear behavior of plane and space truss.

• Steel and Composite Structures
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• v.1 no.4
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• pp.459-480
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• 2001

The damage suffered by steel structures during the Northridge (1994) and Kobe (1995) earthquakes indicates that the fully restrained (FR) connections in steel frames did not behave as expected. Consequently, researchers began studying other possibilities, including making the connections more flexible, to reduce the risk of damage from seismic loading. Recent experimental and analytical investigations pointed out that the seismic response of steel frames with partially restrained (PR) connections might be superior to that of similar frames with FR connections since the energy dissipation at PR connections could be significant. This beneficial effect has not yet been fully quantified analytically. Thus, the dissipation of energy at PR connections needs to be considered in analytical evaluations, in addition to the dissipation of energy due to viscous damping and at plastic hinges (if they form). An algorithm is developed and verified by the authors to estimate the nonlinear time-domain dynamic response of steel frames with PR connections. The verified algorithm is then used to quantify the major sources of energy dissipation and their effect on the overall structural response in terms of the maximum base shear and the maximum top displacement. The results indicate that the dissipation of energy at PR connections is comparable to that dissipated by viscous damping and at plastic hinges. In general, the maximum total base shear significantly increases with an increase in the connection stiffness. On the other hand, the maximum top lateral displacement $U_{max}$ does not always increase as the connection stiffness decreases. Energy dissipation is considerably influenced by the stiffness of a connection, defined in terms of the T ratio, i.e., the ratio of the moment the connection would have to carry according to beam line theory (Disque 1964) and the fixed end moment of the girder. A connection with a T ratio of at least 0.9 is considered to be fully restrained. The energy dissipation behavior may be quite different for a frame with FR connections with a T ratio of 1.0 compared to when the T ratio is 0.9. Thus, for nonlinear seismic analysis, a T ratio of at least 0.9 should not be considered to be an FR connection. The study quantitatively confirms the general observations made in experimental results for frames with PR connections. Proper consideration of the PR connection stiffness and other dynamic properties are essential to predict dynamic behavior, no matter how difficult the analysis procedure becomes. Any simplified approach may need to be calibrated using this type of detailed analytical study.

• Journal of the Earthquake Engineering Society of Korea
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• v.15 no.3
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• pp.37-43
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• 2011

Reliability of piping systems is essential to the safety of any important industrial facilities. During an earthquake, damage to the piping system can occur. It can also cause considerable economic losses and the loss of life following earthquakes. Traditionally, the study of the secondary system was less important than primary structure system, however it has recently been emerging as a key issue for the effective maintenance of the structural system and to help reduce nonstructural earthquake damage. The primary objectives of this study are to evaluate seismic design requirements and the seismic performance of gas and fire protection piping systems installed in reinforced concrete (RC) buildings. In order to characterize the seismic behavior of the existing piping system in an official building, 10 simulated earthquakes and 9 recorded real earthquakes were applied to ground level and the building system by the newmark average acceleration time history method. The results developed by this research can be used for the improvement of new seismic code/regulatory guidelines of secondary systems as well as the improvement of seismic retrofitting or the strengthening of the current piping system.

• Journal of the Korea Concrete Institute
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• v.20 no.2
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• pp.249-256
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• 2008

With consideration of the ongoing construction of high-rise buildings, it is becoming increasingly important to be able to accurately predict the behavior of them on the stage of design, construction and service. Even though many researchers have developed the analysis method to predict the behavior of high-rise buildings, their studies were based on the two dimensional frame structures composed of line elements such as beams and columns. Recently the high-rise buildings with flat-plate system is widely used because of its advantages. In this study a three dimensional analysis method is developed to analyze the behavior of the high-rise buildings with flat-plate system since it is difficult to model the structural systems reasonably with the existing two dimensional analysis method. The analysis method considered the construction sequence including the temporary work such as installation of form, removal of form, installation of shore, and removal of shore. Line elements were used to describe columns, beams, and shores and plate elements were used to model slabs. The creep and drying shrinkage of concrete were also considered to account for the inelastic behavior of concrete.