• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.145-146
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• 2000

The presence of cross-reacting muramidase in Lactobacillus delbrueckii ssp. bulgaricus ULl2 was shown by using monoclonal antibodies raised against an muramidase-2 of Enterococcus hirae ATCC 9790. The separation of protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Western immunoblot confirmed the presence of one cross-reacting band in Enterococcus hirae with an estimated molecular mass of 80 kDa, L. bulgaricus cultured cells harvested after 4 and 12 h were submitted to different autolysin releasing procedures and the liberated products were allowed to cross-react with muramidase-2 antibodies in order to estimate the efficiency of each treatment. Although the cultured cells harvested after 4 h yielded only a slight immune-reaction in Western immunoblots against these enzyme monoclonal antibodies, a strong signal was observed for the cell walls obtained from the same experimental conditions and treated with Triton X-100 surfactant. The same phenomenon was also observed by light fluorescence microscopy. Immune-labelling followed by optical and electron microscopy have shown that the muramidase-2 of L. bulgaricus ULl2 was essentially localized in the innermost part of the cell wall.(omitted)

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 1997.04a
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• pp.77-87
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• 1997

The earthquake data(M$\geq$4.0) for post-1900 in the Korean Peninsula show temporal variation with active and quiet periods. The pattern is quite similar to northeastern China and Inner Zone of Southwest Japan. Yeongweol earthquake occurred in the seismic gap region of the Korean Peninsula. This is the first medium-size earthquake in inland region of the southern peninsula since 1978. The intensity based on the felt area estimation of about 400 places shows MMI III-Ⅷ in inland region. IIon Cheju Island and Ion Ulreung Island. The isoseismal of MMI Ⅶ shows an elongated circle in the direction of NE-SW and covers some parts of Jungdong-myon, Yeongweol-kun, Sindong-eup and Nam-myun, Jeongseon-kun. There occurred quite strong shaking, numerous cracks on the walls of buildings, falling and movement of slate and tiles on the roofs, falling of tiles from the wall and falling of materials from desks, rook falling from mountain and collapse of gravel lauers on the river side. The least square fitting of the intensity data of the Yeongweol earthquake by a popular intensity attenuation relation yields the following : I=Io＋1.82249 - 0.65295*InR - 0.00707*R

• Korean Journal of Veterinary Research
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• v.46 no.3
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• pp.271-274
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• 2006

A 2-years-old female domesticated mouflon with a clinical history of chronic diarrhea and emaciation was submitted to NVRQS. Grossly, there were severe thickening of small intestine wall and enlargement of mesenteric lymph nodes. Microscopically, severe granulomatous inflammation was found in small and large intestine, mesenteric lymph nodes, spleen and liver. By Ziehl-Neelsen stain, innumerable acid-fast rod bacteria were found in the cytoplasm of epitheloid and Langhans type giant cells present in these organs. By PCR assay with primer pair specific for Mycobacterium avium subspecies paratuberculosis(IS 900) with small intestine sample, strong positive reaction was detected, although the organism was not isolated from this organ. Based on the results of histopathology and PCR, we concluded that the case was a typical paratuberculosis in mouflon. As far as we know, this is the first case report of paratuberculosis in mouflon Korea.

• Environmental Analysis Health and Toxicology
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• v.8 no.3_4
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• pp.7-12
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• 1993

An Actinomycete strain isolated from Mt. Dea-Dun had a strong antifungal activity. The culture brith produced by isolated strain showed only antifungal activity against fungi with the exception of yeast and bacteria. It was heat stable, dissolved in ehtylacetate. The concentrated antifungal agent showed cytotoxicity against HEP-2 and HeLa as tumor cell line, and showed weak cytotoxicity against VERO 36 as normal cell line. Morphological and physiological characteristics were tested with isolated strain. The spore color of isolated strain was gray. It had a short chain and produced brown colored lytic substance in yeast extract-malt agar. The cell wall of isolated strain was composed of meso-DAP, and we suggested it as genus Actinomadura. In the existing of chemical inhibitor, isolated strain grew on the condition of 0.0001% crystal violet, 0.1% phenol, 0.01% sodium azide and 10% sodium chloride. Carbon utilization of isolated strain was shown that glucose, sucrose, manitol and sodium citrate were well utilized.

• International Journal of Fluid Machinery and Systems
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• v.4 no.1
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• pp.57-66
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• 2011

Radial-vaned air separators show a strong stall suppression effect in an axial flow fans. From a survey of existing literature on the effects and the author's data, a possible mechanism for the significant effects has been proposed here. The stall suppression is suggested to have been achieved by a combination of the following several effects; (1) suction of blade and casing boundary layers and elimination of embryos of stall, (2) separation and straightening of reversed swirling flow from the main flow, (3) induction of the fan main flow toward the casing wall and enhancement of the outward inclination of meridional streamlines across the rotor blade row, thus keeping the Euler head increase in the decrease in fan flow rate, and (4) reinforcement of axi-symmetric structure of the main flow. These phenomena have been induced and enhanced by a stable vortex-ring encasing the blade tips and the air separator. These integrated effects appear to have caused the great stall suppression effect that would have been impossible by other types of stall prevention devices. Thus the author would like to name the device "tip-vortex-ring assisted stall suppression device".

• Transactions of the Korean Society of Mechanical Engineers B
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• v.21 no.10
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• pp.1273-1283
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• 1997

Experiments of shock wave/turbulent boundary layer interaction were conducted by using a supersonic wind tunnel. Nominal Mach number was varied in the range of 1.6 to 3.0 by means of different nozzles. The objective of the present study is to investigate the effects of boundary layer flow bleed on the interaction flow field in a straight tube. Two-dimensional slits were installed on the tube walls to bleed the turbulent boundary layer flows. The bleed flows were measured by an orifice. The ratio of the bleed mass flow to main mass flow was controlled within the range of 11 per cent. The wall pressures were measured by the flush mounted transducers and Schlieren optical observations were made for almost all of the experiments. The results show that the boundary layer flow bleed reduces the multiple shock waves to a strong normal shock wave. For the design Mach number of 1.6, it was found that the normal shock wave at the position of the silt was resulted from the main flow choking due to the suction of the boundary layer flow.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.22 no.9
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• pp.1277-1289
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• 1998

The objective of the present study is to investigate experimentally the mean flow characteristics of the three-dimensional turbulent boundary layer over a rotating disk with an impinging jet at the center of the disk, which may be regarded as one of the simplest models for the flow in turbomachinery. A relatively strong radial outflow (crossflow) generated from the impinging jet is added to the radial outflow (crossflow) induced by the centrifugal force in order to create the three-dimensional boundary layer. A new calibration technique has been introduced to determine the velocity direction and magnitude using an I-wire probe, where the uncertainties are ${\pm}1.5^{\circ}$ and ${\pm}0.35\;m/s$, respectively, in the laminar boundary layer region, compared with the known exact solutions. The flow in the tangential direction is of similar type to that associated with a favorable pressure gradient, considering that no wake region appears in wall coordinate velocity profiles and the Clauser shape factor is between 4.0 and 5.3. The flow angle is significantly changed by the crossflow generated by the impinging jet.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.32 no.9
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• pp.645-651
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• 2008

For a high speed train driving at 300 km/h, aero-acoustic noise is a dominant component among various noise sources. The aeroacoustic noise is mainly due to inter-coach spacings because discontinuities in the train surface significantly disturb turbulent flows. This often leads to the uncomfortableness of passengers. Interestingly, the aero-acoustic noise reduces with decreasing the mud-flap spacing of the inter-couch spacing. We perform numerical simulations to investigate flow characteristics around the inter-coach spacing. We model the inter-coach spacing as a simple 2-D cavity with flaps, and calculate the velocity and pressure field using two equation turbulence models, varying the flap spacing. The results show that a wider flap spacing develops a higher inflection point in mean velocity profiles over the cavity. It is likely that large eddies generated near the inflection point persist longer in the downstream since they are less affected by the wall. This probably induces the more aero-acoustic noises. The wider spacing also results in the larger pressure difference between the inside and outside of the cavity. This is also responsible for the increased noise since the large difference would cause a strong flow oscillations in and out of the cavity.

• Journal of Magnetics
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• v.22 no.2
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• pp.181-187
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• 2017

Flow of electrically conducting nanofluids is of pivotal importance in countless industrial and medical appliances. Fluctuations in thermophysical properties of such fluids due to variations in temperature have not received due attention in the available literature. Present investigation aims to fill this void by analyzing the flow of copper-water nanofluid with temperature dependent viscosity past a Riga plate. Strong wall suction and viscous dissipation have also been taken into account. Numerical solutions for the resulting nonlinear system have been obtained. Results are presented in the graphical and tabular format in order to facilitate the physical analysis. An estimated expression for skin friction coefficient and Nusselt number are obtained by performing linear regression on numerical data for embedded parameters. Results indicate that the temperature dependent viscosity alters the velocity as well as the temperature of the nanofluid and is of considerable importance in the processes where high accuracy is desired. Addition of copper nanoparticles makes the momentum boundary layer thinner whereas viscosity parameter does not affect the boundary layer thickness. Moreover, the regression expressions indicate that magnitude of rate of change in effective skin friction coefficient and Nusselt number with respect to nanoparticles volume fraction is prominent when compared with the rate of change with variable viscosity parameter and modified Hartmann number.

• Journal of the Korean Magnetics Society
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• v.27 no.1
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• pp.35-40
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• 2017

Kerr microscopy was assembled to observe magnetic domain image of ultra thin 3 %Si-Fe by using parts of an optical microscope. Digital images were obtained from CCD camera attached to the microscopy. A method was suggested to decide a boundary between magnetic domain regions in this study. The method was using some operations such as subtraction, integration and least mean square approximation for pixel values in the digital image. The method has a strong point that high priced image processor is not needed in the Kerr microscopy system. From the results that three different domain walls were observed and magnetic flux density of 0.085 [T], this method could be applied in the magnetic domain regions having a straight $180^{\circ}$ domain wall.