• The Korean Journal of Physiology and Pharmacology
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• v.4 no.3
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• pp.227-234
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• 2000

Many reports suggest that neurotensin (NT) in the gastrointestinal tract may play a possible role as a neurotransmitter, a circulating hormone, or a modulator of motor activity. NT exerts various actions in the intestine; it produces contractile and relaxant responses in intestinal smooth muscle. This study was designed to investigate the effect of NT on motility of antral circular muscle strips in guinea-pig stomach. To assess the role of $Ca^{2+}$ influx in underlying mechanism, slow waves were simultaneously recorded with spontaneous contractions using conventional intracellular microelectrode technique. At the concentration of $10^{-7}$ M, where NT showed maximum response, NT enhanced the magnitude $(863{\pm}198%,\;mean\;SEM,\;n=13)$ and the frequency $(154{\pm}10.3%,\;n=11)$ of spontaneous contractions. NT evoked a slight hyperpolarization of membrane potential, tall and steep slow waves with abortive spikes $(278{\pm}50%,\;n=4).$ These effects were not affected by atropine $(2\;{\mu}M),$ guanethidine $(2\;{\mu}M)$ and tetrodotoxin (0.2μM). NT-induced contractile responses were abolished in $Ca^{2+}-free$ solution and reduced greatly to near abolition by $10\;{\mu}M$ of verapamil or 0.2 mM of $CdCl_2.$ Verapamil attenuated the effects of NT on frequency and amplitude of the slow waves. Taken together, these results indicate that NT enhances contractility in guinea-pig gastric antral circular muscle and $Ca^{2+}$ influx through the voltage-operated $Ca^{2+}$ channel appears to play an important role in the NT-induced contractile mechanism.

• The KIPS Transactions:PartD
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• v.12D no.2 s.98
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• pp.179-188
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• 2005

Much research for spatial join has been extensively studied over the last decade. In this paper, we focus on the filtering step of candidate objects for spatial join operations on the input tables that none of the inputs is indexed. In this case, many algorithms has presented and showed excellent performance over most spatial data. However, if data sets of input table for the spatial join ale skewed, the join performance is dramatically degraded. Also, little research on solving the problem in the presence of skewed data has been attempted. Therefore, we propose a spatial hash strip join (SHSJ) algorithm that combines properties of the existing spatial hash join (SHJ) algorithm based on spatial partition for input data set's distribution and SSSJ algorithm. Finally, in order to show SHSJ the outperform in uniform/skew cases, we experiment SHSJ using the Tiger/line data sets and compare it with the SHJ algorithm.

• Structural Monitoring and Maintenance
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• v.1 no.2
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• pp.183-195
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• 2014

Scour is one of the leading causes of overwater bridge failures worldwide. While monitoring systems have already been implemented or are still being developed, they suffer from limitations such as high costs, inaccuracies, and low reliability, among others. Also, most sensors only measure scour depth at one location and near the pier. Thus, the objective is to design a simple, low cost, scour hole topography monitoring system that could better characterize the entire depth, shape, and size of bridge scour holes. The design is based on burying a robust, waterproofed, piezoelectric sensor strip in the streambed. When scour erodes sediments to expose the sensor, flowing water excites it to cause the generation of time-varying voltage signals. An algorithm then takes the time-domain data and maps it to the frequency-domain for identifying the sensor's resonant frequency, which is used for calculating the exposed sensor length or scour depth. Here, three different sets of tests were conducted to validate this new technique. First, a single sensor was tested in ambient air, and its exposed length was varied. Upon verifying the sensing concept, a waterproofed prototype was buried in soil and tested in a tank filled with water. Sensor performance was characterized as soil was manually eroded away, which simulated various scour depths. The results confirmed that sensor resonant frequencies decreased with increasing scour depths. Finally, a network of 11 sensors was configured to form a distributed monitoring system in the lab. Their exposed lengths were adjusted to simulate scour hole formation and evolution. Results showed promise that the proposed sensing system could be scaled up and used for bridge scour topography monitoring.

• Advances in aircraft and spacecraft science
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• v.4 no.4
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• pp.353-369
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• 2017

Flutter is a dangerous phenomenon encountered in flexible structures subjected to aerodynamic forces. This includes aircraft, buildings and bridges. Flutter occurs as a result of interactions between aerodynamic, stiffness, and inertia forces on a structure. In an aircraft, as the speed of the flow increases, there may be a point at which the structural damping is insufficient to damp out the motion which is increasing due to aerodynamic energy being added to the structure. This vibration can cause structural failure, and therefore considering flutter characteristics is an essential part of designing an aircraft. Scientists and engineers studied flutter and developed theories and mathematical tools to analyze the phenomenon. Strip theory aerodynamics, beam structural models, unsteady lifting surface methods (e.g., Doublet-Lattice) and finite element models expanded analysis capabilities. Periodic Structures have been in the focus of research for their useful characteristics and ability to attenuate vibration in frequency bands called "stop-bands". A periodic structure consists of cells which differ in material or geometry. As vibration waves travel along the structure and face the cell boundaries, some waves pass and some are reflected back, which may cause destructive interference with the succeeding waves. This may reduce the vibration level of the structure, and hence improve its dynamic performance. In this paper, for the first time, we analyze the flutter characteristics of a wing with a periodic change in its sandwich construction. The new technique preserves the external geometry of the wing structure and depends on changing the material of the sandwich core. The periodic analysis and the vibration response characteristics of the model are investigated using a finite element model for the wing. Previous studies investigating the dynamic bending response of a periodic sandwich beam in the absence of flow have shown promising results.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.5
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• pp.2903-2908
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• 2013

Background: Various oncogenes related to cancer have been extensively studied and several polymorphisms have been found to be associated with breast cancer. The current report outlines analysis of germ-line polymorphisms for C677T, A1298C (MTHFR), Leiden, R2 (FV) and 5G/4G (PAI-1) in Turkish breast cancer patients. We studied 51 cases diagnosed with invasive ductal and operable with lymph node-positive breast cancer and 106 women as a control group. Materials and Methods: Peripheric blood-DNA samples were used for genotyping by StripAssay technique which is based on the reverse-hybridization principle and real-time PCR methods and results were compared statistically. Results: The frequency of the MTHFR gene 677T and 1298A alleles were significantly higher in cancer patients than in the healthy subjects. The T allele frequency in codon 677 was 2.3-fold and C allele frequency was 3.1-fold increased in BC when compared to the control group for the MTHFR gene. Both differences were statistically significant (OR: 2.295, CI: 1.283-4.106), p<0.006 and (OR: 3.131, CI:1.826-5.369), p<0.0001 respectively. The R2 allele frequency of FV gene was 5.1-fold increased in the current BC when compared to the control group and that difference was also statistically significant (OR: 5.133, CI: 1.299-20.28), p<0.02. Conclusions: The present data suggest that germ-line polymorphisms of C677T, C1298A for MTHFR and R2 for FV are associated in breast cancer and may be additional prognostic markers related to breast cancer survival. The results now need to be confirmed in a larger group of patients.

• Spatial Information Research
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• v.12 no.4 s.31
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• pp.351-370
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• 2004

At present, environmental monitoring of linear infrastructure is based mainly on field sampling. The 'integrated mapping' approach has received only limited attention from field scientists. The increased environmental regulation of corridor targets has required remote sensing research to develop a sensor or technique for targets ranging from 15 m to 100 m in swath width. In an attempt to identify the optimal remote sensing system for linear targets, an overview is provided of the application requirements and the technology currently available. The relative limitation of traditional remote sensing systems in such a linear application is briefly discussed. It is noted that airborne video could provide, in a cost-effective manner, information required for a very narrow and long strip target utilising the narrow view angle and dynamic stereo coverage. The value of this paper is warranted in proposing a new concept of video infrastructure monitoring as a future research direction in the recognition of sensor characteristics and limitations.

• Archives of Pharmacal Research
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• v.18 no.1
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• pp.12-17
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• 1995

It is essential to clone the preptide transporter in order to obtain better understanding of its molecular structure, regulation, and substrate specificity. Characteristics of an endogenous peptide transporter in oocytes were studied along with expression of an exogenous protor/peptide cotransporter from rabbit intestine. And further efforts toward cloning the transporter were performed. The presence of an endogenous peptide transporter was detected in Xenopus laevis oocytes by measuring the uptake of $0.25/{mu}M(10{\;}{\mu}Ci/ml)[^3H]$-glycylsarcosine (Gly-Sar) at pH 5.5 with or without inhibitors. Yptake of Gly-Sae in oocytes was significantly inhibited by $25{\mu}M$ glycine nd sarcosine. This result suggests that a selective transporter is involved in the endogneous uptake of dipeptides. Collagenase treatment of oocytes used to strip oocytes from ovarian follicles did not affect the Gly-Sar uptake. Changing pH from 5.5 to 7.5 did not affect the Gly-Sae uptake significantly, suggesting no depedence of the endogenous transporter on a transmembrane proton gradient. An exogenous $H^+/pep-tide$ contransported was expressed after microinjection of polyadenylated messenger ribonucleic acid $[poly(A)^+ -mRNA]$ obtained from rabbit small intestine. The Gly-Sar uptake in mRNA-injected oocytes was 9 times thigher than that in water-injected oocyltes. Thus, frog occytes can be utilized fro expression cloning of the genes encoding intestinal $H^+$peptide contransporters. Size fractionation of mRNA was successfully obtained using this technique.

• Journal of Microbiology and Biotechnology
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• v.26 no.8
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• pp.1457-1463
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• 2016

Vibrio anguillarum, a devastating pathogen causing vibriosis among marine fish, is prevailing in worldwide fishery industries and accounts for grievous economic losses. Therefore, a rapid on-site detection and diagnostic technique for this pathogen is in urgent need. In this study, two mouse monoclonal antibodies (MAbs) against V. anguillarum, 6B3-C5 and 8G3-B5, were generated by using hybridoma technology and their isotypes were characterized. MAb 6B3-C5 was chosen as the detector antibody and conjugated with quantum dots. Based on MAb 6B3-C5 labeled with quantum dots, a modified dot blot assay was developed for the on-site determination of V. anguillarum. It was found that the method had no cross-reactivity with other than V. anguillarum bacteria. The detection limit (LOD) for V. anguillarum was 1 × 10³ CFU/ml in cultured bacterial suspension samples, which was a 100-fold higher sensitivity than the reported colloidal gold immunochromatographic test strip. When V. anguillarum was mixed with turbot tissue homogenates, the LOD was 1 × 10³ CFU/ml, suggesting that tissue homogenates did not influence the detection capabilities. Preenrichment with the tissue homogenates for 12 h could raise the LOD up to 1 × 10² CFU/ml, confirming the reliability of the method.

• Journal of Korean Society on Water Environment
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• v.39 no.4
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• pp.316-328
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• 2023

In this study, the characteristics of nonpoint pollutant outflow and contribution rate of pollution in Songya-stream mainstream and tributaries were analyzed. Further, water pollution management and improvement measures for pollution-oriented rivers were proposed. An on-site investigation was conducted to determine the inflow of major pollutants into the basin, and it was found that pollutants generated from agricultural land and livestock facilities flowed into the river, resulting in a high concentration of turbid water. Based on the analysis results of the pollution load data calculated through actual measurement monitoring (flow and water quality) and the occurrence and emission load data calculated using the national pollution source survey data, the S3 and S6 were selected as the concerned pollution tributaries in the Songya-stream basin. Results of cluster analysis using Pearson correlation coefficient evaluation and Density based spatial clustering of applications with noise (DBSCAN) technique showed that the S3 and S6 were most consistent with the C2 cluster (a cluster of Songya-stream mainstream owned area) corresponding to the mainstream of Songya-stream. The analysis results of the major pollutants in the concerned pollution tributaries showed that livestock and land pollutants were the major pollutants. Consequently, optimal management techniques such as fertilizer management, water gate management in paddy, vegetated filter strip and livestock manure public treatment were proposed to reduce livestock and land pollutants.

• Journal of Electrochemical Science and Technology
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• v.14 no.1
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• pp.1-14
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• 2023

The overuse of pesticides in agricultural sectors exposes people to food contamination. Pesticides are toxic to humans and can have both acute and chronic health effects. To protect food consumers from the adverse effects of pesticides, a rapid monitoring system of the residues is in dire need. Molecularly imprinted polymer (MIP) on a screen-printed electrode (SPE) is a leading and promising electrochemical sensing approach for the detection of several residues including pesticides. Despite the huge development in analytical instrumentation developed for contaminant detection in recent years such as HPLC and GC/MS, these conventional techniques are time-consuming and labor-intensive. Additionally, the imprinted SPE detection system offers a simple portable setup where all electrodes are integrated into a single strip, and a more affordable approach compared to MIP attached to traditional rod electrodes. Recently, numerous reviews have been published on the production and sensing applications of MIPs however, the research field lacks reviews on the use of MIPs on electrochemical sensors utilizing the SPE technology. This paper presents a distinguished overview of the MIP technique used on bare and modified SPEs for the detection of pesticides from four recent publications which are malathion, chlorpyrifos, paraoxon and cyhexatin. Different molecular imprint routes were used to prepare these biomimetic sensors including solution polymerization, thermal polymerization, and electropolymerization. The unique characteristics of each MIP-modified SPE are discussed and the comparison among the findings of the papers is critically reviewed.