• Animal Bioscience
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• 제36권7호
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• pp.1120-1129
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• 2023

Objective: This study aimed to determine the protective efficacy of Buddha's Temple (BT) extract against tert-butyl hydroperoxide (t-BHP)-induced oxidative stress in Gallus gallus chicken embryo fibroblast cell line (DF-1) and its effects on the cell lipid metabolism. Methods: In this experimental study, Gallus gallus DF-1 fibroblast cells were pretreated with BT 10^-7 for 24 hours, followed by their six-hour exposure to t-BHP (100 μM). Water-soluble tetrazolium salt-8 (WST-8) assays were performed, and the growth curve was computed. The intracellular gene expression changes caused by BT extract were confirmed through quantitative polymerase chain reaction (qPCR). Flow cytometry, oil red O staining experiment, and thin-layer chromatography were performed for the detection of intracellular metabolic mechanism changes. Results: The WST-8 assay results showed that the BT pretreatment of Gallus gallus DF-1 fibroblast cell increased their cell survival rate by 1.08%±0.04%, decreased the reactive oxygen species (ROS) level by 0.93%±0.12% even after exposure to oxidants, and stabilized mitochondrial activity by 1.37%±0.36%. In addition, qPCR results confirmed that the gene expression levels of tumor necrosis factor α (TNFα), TIR domain-containing adapter inducing IFN-beta (TICAM1), and glucose-regulated protein 78 (GRP78) were regulated, which contributed to cell stabilization. Thin-layer chromatography and oil red O analyses showed a clear decrease in the contents of lipid metabolites such as triacylglycerol and free fatty acids. Conclusion: In this study, we confirmed that the examined BT extract exerted selective protective effects on Gallus gallus DF-1 fibroblast cells against cell damage caused by t-BHP, which is a strong oxidative inducer. Furthermore, we established that this extract significantly reduced the intracellular ROS accumulation due to oxidative stress, which contributes to an increase in poultry production and higher incomes.

• 항공우주시스템공학회지
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• 제1권2호
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• pp.27-36
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• 2007

The Damage Tolerant Design is developed to help alleviate structural failure and cracking problems in aerospace structures. Recently, the Damage Tolerant Design is required and recommended for most of aircraft design. In this paper, the damage tolerant design is applied to tilt rotor UAV. First of all, the fatigue load spectrum for the tilt rotor UAV is developed and fatigue analysis is performed for the flaperon joint which has FCL (fatigue critical location). Tilt rotor UAV has two modes: helicopter mode when UAV is taking off and landing; fixed wing mode when the tilt rotor UAV is cruising. To make fatigue load spectrum, FELIX is used for helicopter mode. TWIST is used for fixed wing mode. Fatigue analysis of flaperon joint is performed using fatigue load spectrum. E-N curve approach is used for picking crack initiation point. The LEFM(Linear Elastic Fracture Method) is considered for analyzing crack growth or propagation. Finally, including the crack initiation and propagation, the fatigue life is evaluated. Therefore the Damage Tolerant Design can be done.

• Elastomers and Composites
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• 제33권3호
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• pp.168-176
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• 1998

자동차의 고무부품 중의 하나인 스트라트방진고무의 특성 평가를 위하여 유한요소해석과 피로 수명시험을 실시하였다. 유한요소해석에 필요한 변형률에너지함수의 계수를 결정하기 위하여 인장, 압축 및 전단시험을 실시하였으며, 유한요소해석 결과로부터 변형 양성 및 취약 부위를 예측하였다. 부품의 피로수명시험 결과로부터 하중-수명 관계를 얻었으며, 피로파손 부위는 유한요소해석에서 예측된 취약 부위와 잘 일치하였다.

• 대한기계학회논문집A
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• 제24권9호
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• pp.2183-2190
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• 2000

A thermal aging is observed in a primary reactor cooling system(RCS) made of a casting stainless steel when the RCS is exposed for long period at the reactor operating temperature, 290~3300C An investigation of effects of thermal aging on a low cycle fatigue characteristics included a stress variations caused by a reactor operation and trip, is required. The purpose of the present investigation is to find an effect of a thermal aging of the CF8M on a low cycle fatigue life. The specimen of CF8M are prepared by an artificially accelerated aging technique holding 300 and 1800hr at 4300C respectively. The low cycle fatigue tests for the virgin and two aged specimens are performed at the room temperature for various strain amplitudes($\varepsilon$ta), 0.3, 0.5, 0.8, 1.0, 1.2 and 1.5% strain. Through the experiment, it is found that the fatigue life is rapidly reduced with an creasing of the aging time. The experimental fatigue life estimation formulas between the virgin and two aged specimen are obtained and are proposed to a analysis purpose.

• 대한토목학회논문집
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• 제6권3호
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• pp.53-62
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• 1986

소재시험편(素材試驗片)과 맞대기 용접시험편(鎔接試驗片)을 대상(對象)으로 피로시험(疲勞試驗)을 행(行)하여 작용최대응력(作用最大應力)-피로수명(疲勞壽命)(S-N)선도(線圖), 소성변형율(塑性變形率)-하중반복회수(荷重反復回數)(${\varepsilon}_p$-N)선도(線圖), 초기균열발생시(初期龜裂發生時)의 소성변형율(塑性變形率)-피로수명(疲勞壽命)(${\varepsilon}_p-N_c$)선도(線圖) 및 균열성장율(龜裂成長率)-응력확대계수변동범위(應力擴大係數變動範圍)(da/dN-${\Delta}K$)선도(線圖)를 그려서 강구조물(鋼構造物) 용접부(鎔接部)에서의 여러가지 피로거동(疲勞擧動)을 검토(檢討)하였다. 본(本) 연구(硏究)에서 얻어진 결과(結果)를 요약(要約)하면 다음과 같다. 하중반복회수(荷重反復回數) $2{\times}10^6$ cycle에서의 피로강도(疲勞强度)는 용접부(鎔接部)가 소재부(素材部)의 약(約) 60%였으며 강복응력(降伏應力)에 대한 피로강도(疲勞强度)의 비(比)는 소재부(素材部)와 용접부(鎔接部)가 각각(各各) 0.72, 0.65로 나타났다. 용접시험편(鎔接試驗片)의 S-N 선도(線圖)는 피로강도(疲勞强度)의 감소(減少)가 완만(緩慢)한 구간(區間)과 피로강도(疲勞强度)의 감소(減少)가 큰 구간(區間)으로 분류(分類)되어 작용최대응력(作用最大應力)이 작을 수록 피로강도(疲勞强度)의 감소(減少)가 커지는 현상(現象)을 보였다. 두 구간(區間)으로 구분(區分)되지 않은 S-N 선도(線圖)에 의한 피로강도(疲勞强度)에 비(比)해 $2{\times}10^6$ cycle에서의 피로강도(疲勞强度)가 약(約) 83%에 해당(該當)하였다. 이는 낮은 응력(應力)에서 용접부(鎔接部) 내부결함(內部缺陷)의 영향(影響)이 크게 작용(作用)하는 때문이 아닌가 생각된다. 용접시험편(鎔接試驗片)의 경우(境遇) 소재시험편(素材試驗片)에 비(比)해, 또 작용최대응력(作用最大應力)이 클수록 초기균열발생(初期龜裂發生)이 빠르고 균열발생직전(龜裂發生直前)의 소성변형율(塑性變形率)의 증가(增加)가 급격(急激)한 것을 알 수 있었다. 소재시험편(素材試驗片)에서 ${\varepsilon}_p-N_c$ 관계식(關係式)의 상수(常數) ${\alpha}$값이 0.42 로 Manson, Coffin의 연구결과(硏究結果)와 거의 일치(一致)하였고 용접시험편(鎔接試驗片)에서는 이 값이 0.28 로 이들의 연구결과(硏究結果)와 큰 차이(差異)를 나타내었는데, 이는 피로(疲勞)에 의한 용접부(鎔接部) 소성변형(塑性變形)의 급격(急激)한 변화(變化)와 내부결함(內部缺陷)의 영향(影響)이 작용(作用)했기 때문이 아닌가 생각된다. 이와 같은 결과(結果)로 볼 때 기존(旣存)의 강구조물(鋼構造物) 및 향후(向後) 신설(新設)될 강구조물(鋼構造物) 용접부(鎔接部)의 품질(品質) 및 피로(疲勞)에 대한 안전성(安全性)에 적절(適切)한 대책(對策)이 요망(要望)되며, 이 분야(分野)에 관한 더 많은 연구(硏究)가 행(行)해져야 되리라고 사료(思料)된다.

• 대한용접접합학회:학술대회논문집
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• 대한용접접합학회 2002년도 Proceedings of the International Welding/Joining Conference-Korea
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• pp.139-144
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• 2002

Ti-50.9at%Ni wires were welded using pulsed YAG laser. The laser welded wires were tested for investigating the shape memo교 effect and the ability of super elasticity. The fatigue properties of the welded wires were investigated using the rotary bending fatigue tester specially designed for wires. Moreover, the effect of defocusing distance during laser welding on the static and fatigue properties was investigated. The shape memory effect and super elasticity of the laser welded wires were approximately identical with that of base metal at the test temperature below 353K. However, the welded wires were broken within elastic limit at the test temperature above 353k. Under the cyclic bending loading conditions, the welded wires could be useful only below the elastic limit, while the base metal had sufficient fatigue life even the stress induced M-phase region. The fatigue strength of the welded wires was about half of that of the base metal. The deterioration of the static and fatigue properties in the welded wires was proven to be from the large difference of the transformation behavior between the base metal and welded part that is caused by vaporization of Ni-content at the welded part during the welding process. The defocusing distance below 3mm acted more largely on lowering the strength of the welded wires than that of 6mm or 8mm.

• International Journal of Korean Welding Society
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• 제3권1호
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• pp.39-44
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• 2003

The welded specimens were made by butt welding of the 2 wires of 50mm length using the pulsed YAG laser. The laser welded wires were tested for investigating the shape memory effect and the ability of super elasticity. The fatigue properties of the welded wires were investigated using the rotary bending fatigue tester specially designed for wires. Moreover, the effect of defocusing distance during laser welding on the static and fatigue properties was Investigated. The shape memory effect and super elasticity of the laser welded wires were approximately identical with that of base metal at the test temperature below 353K. However, the welded wires were broken within elastic limit at the test temperature above 353k. Under the cyclic bending loading conditions, the welded wires could be useful only below the elastic limit, while the base metal had sufficient fatigue life even the stress induced M-phase region. The fatigue strength of the welded wires was about half of that of the base metal. The deterioration of the static and fatigue properties in the welded wires was proven to be from the large difference of the transformation behavior between the base metal and welded part that is caused by vaporization of Ni-content at the welded part during the welding process. The defocusing distance below 3mm acted more largely on lowering the strength of the welded wires than that of 6mm or 8mm.

• 한국터널지하공간학회 논문집
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• 제22권5호
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• pp.529-541
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• 2020

기계화터널시공의 대표적인 장비인 TBM의 커터헤드는 타 장비에 비해 굴착 중 발생하는 하중이 매우 크며, 마모가 발생하여 단면이 손실되는 작업환경을 가지고 있어 피로파괴에 의한 설계검토가 필요하지만, TBM커터헤드에 대한 피로해석을 수행한 사례는 찾기 어렵다. 본 연구에서는 직경 8.2 m인 커터헤드를 대상으로 안전수명설계 개념으로 S-N커브를 이용하여 응력-수명 설계 검토를 수행하였다. 또한 건설장비의 피로설계방법과 피로손상도를 평가하는 방법에 대해 소개하고 직경 8.2 m의 TBM 커터헤드를 대상으로 피로해석을 수행한 결과를 설명하였다. S-N curve는 피로 설계를 하는 데에 있어서 핵심적인 역할을 하는 것을 알 수 있었으며, 피로 하중을 받고 있는 구조물이 현재 시점에서 어느 정도의 피로 손상을 받고 있는지를 평가하는 데에도 사용될 수 있다. 앞으로 건설장비에서도 장비를 사용하는 동안 어떤 시점에서 피로문제가 발생하는지와 장비의 안전 점검은 언제 실시하는 것이 효과적인지 등에 대한 정보를 파악하는 안전수명설계 개념을 도입하는 것이 필요하다.

• 대한기계학회논문집
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• 제10권4호
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• pp.477-486
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• 1986

본 연구에서는 SM20C를 모재로 하여 입경의 크기가 다른 3종의 복합조직강을 제작 동일한 분위기에서 저 및 고사이클 전영역에 걸쳐 피로특성을 검토하고져 한다. 제일보는 그 중 저사이클특성에 대한 보고이다. 일반적으로 저사이클 피로현상은 재 료가 탄소성 상태하에서 전위, 미소크랙, 보이드(void) 등의 인자가 복합적으로 작용 하여 발생함으로 변형률속도, 제어파형, 온도, 시험방법 및 분위기에 따라 많은 영향 을 받는다. 따라서 본 연구에서는 두가지 실험방법을 사용, 응력-변형율거동을 검토 복합조직강의 피로특성과 입경크기가 피로거동 및 강도에 미치는 영향을 비교 고찰하 였다.

• 한국안전학회지
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• 제24권3호
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• pp.19-24
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• 2009

Currently the technique of composite material field is developed day by day. The many kinds industrial facility and life articles are coming to make with the composite material. But still the engineering works field the building and the bridge uses the material which is old era and is making. To here there is a various problem but the biggest problem the theory of the composite material is complicated too and means that the application is been delayed about constructive structure. When the composite material is used widely from constructive field, too with difficult theory in technical expert and easily with the research for the experiment data accumulation is necessary. The tensile and fatigue test of the carbon/epoxy which is a high-class composite material led from the present paper consequently and the change of the intensity coefficient which follows in repeated load researched.