• 지역사회간호학회지
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• 제24권3호
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• pp.255-263
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• 2013

Purpose: This study examined the fitness of a path model for the relationship among daily life stress, problem of life, social support, depression, and suicidal ideation in adolescents. Methods: The subjects consisted of 247 adolescents. Data were collected through self-report questionnaires. The data were analyzed by the SPSS and AMOS programs. Results: Daily life stress, problem of life, social support, and depression showed direct effects on suicidal ideation in adolescents, while daily life stress and problem of life showed indirect effects on suicidal ideation in adolescents. Daily life stress and problem of life showed direct effects on social support and depression in adolescents. The hypothetical path model of adolescents' suicidal ideation was proven correct. Conclusion: Based on the outcomes of this study, it is necessary to design an intervention program that emphasizes the reduction of daily life stress, problem of life, depression and enhancement social support in order to prevention adolescents' suicidal ideation.

• Asian-Australasian Journal of Animal Sciences
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• 제33권3호
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• pp.424-435
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• 2020

Objective: The study was conducted to investigate variations in the immunophysiological responses to exercise-induced stress in Jeju and Thoroughbred horses. Methods: Blood samples were collected from the jugular veins of adult Jeju (n = 5) and Thoroughbred (n = 5) horses before and after 30 min of exercise. The hematological, biochemical, and immunological profiles of the blood samples were analyzed. Blood smears were stained and observed under a microscope. The concentration of cell-free (cf) DNA in the plasma was determined using real time polymerase chain reaction (PCR). Peripheral blood mononuclear cells (PBMCs) and polymorphonuclear cells were separated using Polymorphprep, and the expression of various stress-related and chemokine receptor genes was measured using reverse transcriptase (RT) and real-time PCR. Results: After exercise, Jeju and Thoroughbred horses displayed stress responses with significantly increased rectal temperatures, cortisol levels, and muscle catabolism-associated metabolites. Red blood cell indices were significantly higher in Thoroughbred horses than in Jeju horses after exercise. In addition, exercise-induced stress triggered the formation of neutrophil extracellular traps (NETs) and reduced platelet counts in Jeju horses but not in Thoroughbred horses. Heat shock protein 72 and heat shock protein family A (Hsp70) member 6 expression is rapidly modulated in response to exercise-induced stress in the PBMCs of Jeju horses. The expression of CXC chemokine receptor 4 in PBMCs was higher in Thoroughbred horses than in Jeju horses after exercise. Conclusion: In summary, the different immunophysiological responses of Jeju and Thoroughbred horses explain the differences in the physiological and anatomical properties of the two breeds. The physiology of Thoroughbred horses makes them suitable for racing as they are less sensitive to exercise-induced stress compared to that of Jeju horses. This study provides a basis for investigating the link between exercise-induced stresses and the physiological alteration of horses. Hence, our findings show that some of assessed parameters could be used to determine the endurance performance of horses.

• Fisheries and Aquatic Sciences
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• 제13권3호
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• pp.216-223
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• 2010

Abalone (Haliotis discus hannai), mostly distributed and maricultured in southwestern coastal areas of South Korea, is recognized as an economically important species in the fishery industry. Abalone intestines are one of the by-products of abalone processing. To investigate abalone intestines as bioactive substances, abalone intestine gastrointestinal digests (AIGIDs) of various molecular weights (MWs) were prepared using in vitro gastrointestinal digestion and an ultrafiltration system, and tested for inhibitory effects against reactive oxygen species (ROS) and oxidative stress in macrophage cells treated with hydrogen peroxide ($H_2O_2$). In our results, among AIGIDs, AIGID-III (MW=5-10 kDa) showed potent inhibitory activities for lipid peroxidation and free radicals. Additionally, the results clearly indicated that AIGID-III treatment could prevent cytotoxic damage of macrophages by $H_2O_2$-induced oxidative stress due to its potent scavenging ability against cellular ROS. These results suggest that AIGIDs may have protective and therapeutic potential for oxidative stress syndromes and immune diseases through ROS inhibition in macrophage cells.

• 융합정보논문지
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• 제12권3호
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• pp.204-213
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• 2022

본 연구는 대학생들의 구강건강수준에 영향을 미칠 수 있는 스트레스와 턱관절 장애를 조사하고 구강건강 관련 삶의 질과의 관련성을 파악하기 위해 실시하였다. 자료조사는 2021년 6월 3부터 2021년 7월 8일까지 대전 일부지역의 대학생 227명을 대상으로 실시하였으며, 독립표본 t검정, one-way ANOVA, Pearson 상관분석, 다중회귀분석을 이용하여 분석하였다. 조사결과, 턱관절 장애는 모든 스트레스 하위요인과 양의 상관성을 보였으며, 구강건강관련 삶의 질은 모든 스트레스 하위요인 및 턱관절 장애와 음의 상관성을 보였다. 또한, 구강건강관련 삶의 질에는 턱관절 장애와 대인 스트레스 및 가치관 스트레스가 영향을 미치는 것으로 나타났다. 이상의 결과를 토대로 대학생들의 스트레스와 턱관절 장애를 예방하여 구강건강관련 삶의 질을 향상시키는 체계적인 방안 모색이 요구된다.

• 한국콘텐츠학회논문지
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• 제12권7호
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• pp.306-313
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• 2012

남녀 대학생의 스트레스 정도와 식생활관리조사(I)에서는 조사대상자의 일반적 특성, 체중, 신장, BMI, 조사대상자의 성별에 따른 BMI에 의한 비만도, 스트레스를 받는 정도와 지속성, 스트레스를 받는 경우 음식섭취에 관한 사항, 스트레스의 원인과 해소방법에 대해 조사하였다. 연구 (I)에 이어 본 연구에서는 조사대상자의 일반적 특성에 따른 스트레스 시 선호하는 음식 맛과의 관계, 스트레스 해소를 위해 음식 섭취 후 느낌과의 관계, 스트레스와 생활 습관의 관계, 스트레스와 성격과의 관계, 스트레스와 비만도(BMI)의 관계에 대해 조사하였다. 스트레스 시 선호하는 맛은 매운 맛과 단맛이며 스트레스 해소를 위해 음식 섭취 후 느낌은 조사대상자의 연령, 전공, 한 달간 사용 용돈에 따라서는 유의한 차이가 없었다. 수면과 건강상태가 나쁠수록 스트레스가 심하고 운동량, 흡연량, 음주량, 평소 건강에 대한 관심에 따른 스트레스 정도에도 유의적인 차이는 없었다. 또한 성격이 급할수록 스트레스 정도가 심하며 비만도에 따른 스트레스 정도에는 유의적인 차이가 없었다. 이상의 결과로 대학생들의 스트레스 관리를 위한 올바른 식생활과 생활태도 실천에 대한 영양교육이 요구된다.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.138-138
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• 2005

• 한국보건간호학회지
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• 제29권2호
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• pp.312-324
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• 2015

Purpose: This study addressed the relationship among nutritional risk, stress, and health related quality of life for older women with a foreign daughter-in-law. Methods: A descriptive research design was used for this study. The subjects of this study were 112 older women with a foreign daughter-in-law all of whom were over 65 years and living in D city. Personal interview was used for data collection. Nutritional risk, stress, and health related quality of life were measured using Nutrition Screening Initiative (NSI), Family Inventor of Life Events and Changes (FILE), and Medical Outcome Study (MOS) Short-form 36-Item Health Survey (SF-36), respectively. Results: Older women with a foreign daughter-in-law showed negative correlation between quality of life related health and nutritional risk, stress and age, and positive correlation between Activities of Daily Living (ADL); 46% of variance in health related quality of life of older women with a foreign daughter-in-law was explained by nutritional risk, ADL, stress and occupation. Conclusion: Based on the results of this study, we suggested that specialized programs should be established to help in development of social relationship networks for older women who have a foreign daughter-in-law.

• Journal of Ginseng Research
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• 제45권2호
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• pp.218-227
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• 2021

Background: Panax ginseng is one of the most important medicinal plants and is usually harvested after 5 to 6 years of cultivation in Korea. Heavy metal (HM) exposure is a type of abiotic stress that can induce oxidative stress and decrease the quality of the ginseng crop. Siderophore-producing rhizobacteria (SPR) may be capable of bioremediating HM contamination. Methods: Several isolates from ginseng rhizosphere were evaluated by in vitro screening of their plant growth-promoting traits and HM resistance. Subsequently, in planta (pot tests) and in vitro (medium tests) were designed to investigate the SPR ability to reduce oxidative stress and enhance HM resistance in P. ginseng inoculated with the SPR candidate. Results: In vitro tests revealed that the siderophore-producing Mesorhizobium panacihumi DCY119^T had higher HM resistance than the other tested isolates and was selected as the SPR candidate. In the planta experiments, 2-year-old ginseng seedlings exposed to 25 mL (500 mM) Fe solution had lower biomass and higher reactive oxygen species level than control seedlings. In contrast, seedlings treated with 10⁸ CFU/mL DCY119^T for 10 minutes had higher biomass and higher levels of antioxidant genes and nonenzymatic antioxidant chemicals than untreated seedlings. When Fe concentration in the medium was increased, DCY119^T can produce siderophores and scavenge reactive oxygen species to reduce Fe toxicity in addition to providing indole-3-acetic acid to promote seedling growth, thereby conferring inoculated ginseng with HM resistance. Conclusions: It was confirmed that SPR DCY119^T can potentially be used for bioremediation of HM contamination.

• Journal of Microbiology and Biotechnology
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• 제21권9호
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• pp.893-902
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• 2011

Endophytic fungi are little known for their role in gibberellins (GAs) synthesis and abiotic stress resistance in crop plants. We isolated 10 endophytes from the roots of field-grown soybean and screened their culture filtrates (CF) on the GAs biosynthesis mutant rice line - Waito-C. CF bioassay showed that endophyte GMH-1B significantly promoted the growth of Waito-C compared with controls. GMH-1B was identified as Penicillium minioluteum LHL09 on the basis of ITS regions rDNA sequence homology and phylogenetic analyses. GC/MS-SIM analysis of CF of P. minioluteum revealed the presence of bioactive $GA_4$ and $GA_7$. In endophyte-soybean plant interaction, P. minioluteum association significantly promoted growth characteristics (shoot length, shoot fresh and dry biomasses, chlorophyll content, and leaf area) and nitrogen assimilation, with and without sodium chloride (NaCl)-induced salinity (70 and 140 mM) stress, as compared with control. Field-emission scanning electron microcopy showed active colonization of endophyte with host plants before and after stress treatments. In response to salinity stress, low endogenous abscisic acid and high salicylic acid accumulation in endophyte-associated plants elucidated the stress mitigation by P. minioluteum. The endophytic fungal symbiosis of P. minioluteum also increased the daidzein and genistein contents in the soybean as compared with control plants, under salt stress. Thus, P. minioluteum ameliorated the adverse effects of abiotic salinity stress and rescued soybean plant growth by influencing biosynthesis of the plant's hormones and flavonoids.

• Animal cells and systems
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• 제7권4호
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• pp.345-348
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• 2003

The effects of acute acid stress on hatching success and hatching period of laboratory-reared hermaphroditic fish Rivulus marmoratus were examined. The effects of acute acid toxicity on mortality was also determined in three life stages of this fish. There was a significant negative effect of acid stress on hatching performance in the R. marmoratus embryos. The hatching success was only 5% at pH 3.5 compared to over 78% at pH higher than 4.0. The hatching period was also delayed by low pH treatments. The larval and juvenile stages were more sensitive to acid toxicity on mortality than the adult stage, but larvae and juveniles showed similar sensitivity. The 96-h LC50 value was pH 3.8 in larval and juvenile stages and pH 3.3 in adult stage.