• Journal of Ginseng Research
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• v.42 no.2
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• pp.225-228
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• 2018

Our results suggested that thermal stress can lead to activation of hippocampal cell damage and reduction of memory-associated molecules in HT22 cells. These findings also provide a part of molecular rationale for the role of ginsenoside Rg5 as a potent cognitive impairment preventive compound in blocking the initiation of hippocampal damage.

• Proceedings of the Korean Society of Grassland Science Conference
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• 2005.06a
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• pp.208-209
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• 2005

• Journal of the Korean Society of School Health
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• v.16 no.1
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• pp.9-21
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• 2003

The present study was designed to evaluate the factors influential on stress and subjective fatigue symptoms based on school life environments and daily life styles among high school students. The self-administered questionnaires were delivered to 2,381 high school students of both sexes in Taejon Metropolitan city during the period from Mar. 1st to Jun. 30th, 2000. The analysis of study results revealed the following findings: 1. According to the magnitude of stress, the normal subjects were 3.1%, the groups with potential stress were 64.7%, and the groups at high risk for stress were 32.2%. Higher level of stress existed in the female than the male students, and in the third grader than the 1st and 2nd graders. According to the classification of typical constitutional symptoms of fatigue, category III (group with bodily projection of fatigue) was the most frequent and it was followed by category II (group with difficulty in concentration) and category I (group with dullness and sleepiness) in a decreasing order of frequency, which showed that the predominant pattern of fatigue arose from the body parts. 2. With regard to the school life characteristics and stress scores, the higher scores of stress were shown in the groups with the lower grades, with worse friend's relation and with the lower satisfaction with the school life. The scores for the subjective fatigue symptoms were higher in the male, in the low graders, in the better friend's relation, and in the satisfactory group than the respective counterparts. 3. Concerning home life characteristics, the higher scores of stress were associated with the students characterized by the recognized poor economic conditions, lower interests of parents, lack of satisfaction with the home life, the poor subjective health status. On the other hand, the scores for the subjective fatigue symptoms were higher in the student groups with good economic conditions, higher interests of parents, presence of satisfaction with the home life, and good subjective health status. 4. Concerning daily life styles, the higher scores of stress were in the students who had inappropriate sleep hours, skipped breakfasts, daily consumption of intermeal snacks, lack of exercise, daily smoking, normal indices of obesity, and lower indices of health habit. Conversely, the scores of subjective fatigue symptoms were higher in the groups who had daily breakfasts, no intermeal snacks, daily exercise, no smoking than their counterparts. 5. The factors exerting influence upon the stress included the satisfaction with school life, friend's relation, satisfaction with the home life, exercise, school grades, interests of parents, school year, sex, scores of health habit, degree of obesity, economic conditions of home. Those influencing on the degree os stress included stress, intermeal snacks, smoking, friend's relation and satisfaction with the home life.

• Biomolecules & Therapeutics
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• v.29 no.4
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• pp.434-444
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• 2021

BRAF inhibitors are insufficient monotherapies for BRAF-mutated cancer; therefore, we investigated which inhibitory pathway would yield the most effective therapeutic approach when targeted in combination with BRAF inhibition. The oncogenic BRAF inhibitor, PLX4720, increased basal autophagic flux in BRAF-mutated cells compared to wild-type (WT) BRAF cells. Interestingly, early autophagy inhibition improved the effectiveness of PLX4720 regardless of BRAF mutation, whereas late autophagy inhibition did not. Although ATG5 knockout led to PLX4720 resistance in both WT and BRAF-mutated cells, the MEK inhibitor trametinib exhibited a synergistic effect on PLX4720 sensitivity in WT BRAF cells but not in BRAF-mutated cells. Conversely, the prolonged inhibition of endoplasmic reticulum (ER) stress reduced basal autophagy in BRAF-mutated cells, thereby increasing PLX4720 sensitivity. Taken together, our results suggest that the combined inhibition of ER stress and BRAF may simultaneously suppress both pro-survival ER stress and autophagy, and may therefore be suitable for treatment of BRAF-mutated tumors whose autophagy is increased by chronic ER stress. Similarly, for WT BRAF tumors, therapies targeting MEK signaling may be a more effective treatment strategy. Together, this study presents a rational combination treatment strategy to improve the efficacy of BRAF inhibitors depending on BRAF mutation status.

• Korean Journal of Occupational Health Nursing
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• v.28 no.4
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• pp.187-196
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• 2019

Purpose: The purpose of this study was to investigate the association of job stress and health promotion behaviors with the quality of life in female riot police officers. Methods: A cross-sectional design was adopted, and a convenience sample of 182 female riot police officers from 5 Metropolitan Police Agencies in South Korea. All participants completed a self-administered survey questionnaire that assessed job stress, health promotion behavior, and quality of life in May 2018. The collected data were analyzed using t-test, analysis of variance, and correlation and multiple linear regression analyses, all of which were executed using SPSS/WIN 23.0 and STATA 13.0. Results: The level of job stress among participants was moderate, and the mean was 45.25 out of a maximum possible score of 100. The mean scores that emerged for health promotion behaviors and the quality of life were 2.62 (maximum=4) and 56.59 (maximum=100), respectively. Job stress (r=-.380, p<.001) and health promotion behaviors (r=.559, p<.001) were correlated with the quality of life. The statistically significant predictors of the quality of life were job stress (${\beta}=-.212$, p=.001) and health promotion behaviors (${\beta}=.417$, p<.001). Conclusion: The variables that were associated with the quality of life of female riot police officers were job stress and health promotion behaviors. Therefore, interventions that can enhance job stress management and health promotion behaviors are needed to improve the quality of life of female riot police officers.

• The Korean Journal of Physiology and Pharmacology
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• v.24 no.6
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• pp.473-479
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• 2020

Endothelial cell injury is a major contributor to cardiovascular diseases. The 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-Glucoside (TSG) contributes to alleviate human umbilical vein endothelial cells (HUVECs) injury through mechanisms still know a little. This study aims to clarify the TSG effects on gene expression (mRNA and microRNA) related to oxidative stress and endoplasmic reticulum stress induced by H₂O₂ in HUVECs. We found that TSG significantly reduced the death rate of cells and increased intracellular superoxide dismutase activity. At qRT-PCR, experimental data showed that TSG significantly counteracted the expressions of miR-9-5p, miR-16, miR-21, miR-29b, miR-145-5p, and miR-204-5p. Besides, TSG prevented the expression of ATF6 and CHOP increasing. In contrast, TSG promoted the expression of E2F1. In conclusion, our results point to the obvious protective effect of TSG on HUVECs injury induced by H₂O₂, and the mechanism may through miR16/ATF6/ E2F1 signaling pathway.

• Journal of Biosystems Engineering
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• v.41 no.3
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• pp.273-280
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• 2016

Purpose: This experiment was conducted to detect water stress in terms of the moisture content of cucumber seedlings under water stress condition using a hyperspectral image acquisition system, linear regression analysis, and partial least square regression (PLSR) to achieve a non-destructive measurement procedure. Methods: Changes in the reflectance spectrum of cucumber seedlings under water stress were measured using hyperspectral imaging techniques. A model for estimating moisture content of cucumber seedlings was constructed through a linear regression analysis that used the moisture content of cucumber seedlings and a normalized difference vegetation index (NDVI). A model using PLSR that used the moisture content of cucumber seedlings and reflectance spectrum was also created. Results: In the early stages of water stress, cucumber seedlings recovered completely when sub-irrigation was applied. However, the seedlings suffering from initial wilting did not recover when more than 42 h passed without irrigation. The reflectance spectrum of seedlings under water stress decreased gradually, but increased when irrigation was provided, except for the seedlings that had permanently wilted. From the results of the linear regression analysis using the NDVI, the model excluding wilted seedlings with less than 20% (n=97) moisture content showed a precision ($R^2$ and $R^2_{\alpha}$) of 0.573 and 0.568, respectively, and accuracy (RE) of 4.138% and 4.138%, which was higher than that for models including all seedlings (n=100). For PLS regression analysis using the reflectance spectrum, both models were found to have strong precision ($R^2$) with a rating of 0.822, but accuracy (RMSE and RE) was higher in the model excluding wilted seedlings as 5.544% and 13.65% respectively. Conclusions: The estimation model of the moisture content of cucumber seedlings showed better results in the PLSR analysis using reflectance spectrum than the linear regression analysis using NDVI.

• The Journal of Korean Physical Therapy
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• v.33 no.3
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• pp.123-130
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• 2021

Purpose: Despite the existence of Snoezelen method, there is no case of researching the effects on game addiction. Therefore, this study aims to investigate the effects of Snoezelen therapy on stress, depression, anxiety and quality of life of college students who overuse computer games or mobile phone games. Methods: Eight students who responded to spend more than 30 hours of computer or mobile phone games in the selection questionnaire were recruited (The total respondents were 224 students). Students were randomly assigned to either the study group (4 students) or the control group (4 students). Stress (Korean version of Perceived Stress Scale, K-PSS), depression (Korean Screening Tool for Depression Disorders, K-DEP), Anxiety (Korean Beck Anxiety Inventory, K-BAI) and quality of life (Short Form Health Survey version 2 [SF-36]) were assessed three times (before intervention [pre], post-3 weeks [post 1], post-6 weeks [post 2]). Results: First, the study group (-4.75±3.86) and control group (1.00±2.71) showed significant difference (p<0.05) in the analysis of score changes between pre-test and test at 6 weeks (post 2) for K-DEP. Next, pre-test and test at 3 weeks of intervention (post 1) for K-BAI showed significant difference (p<0.05) in the study group (-2.50±1.91) and control group (2.25±2.99). In addition, pre-test and test at 6 weeks (post 2) showed significant difference (p<0.05) for the study group (-3.00±1.51) and control group (4.75±6.24). Conclusion: The study confirmed the possibility of Snoezelen therapy as a therapy method to induce effective responses in stress, depression, anxiety, and quality of life of college students with game addiction.

• Development and Reproduction
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• v.22 no.1
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• pp.73-83
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• 2018

This study investigates the endoplasmic reticulum (ER) stress and subsequent apoptosis in duced during somatic cell nuclear transfer (SCNT) process of porcine SCNT embryos. Porcine SCNT and in vitro fertilization (IVF) embryos were sampled at 3 h and 20 h after SCNT or IVF and at the blastocyst stage for mRNA extraction. The x-box binding protein 1 (Xbp1) mRNA and the expressions of ER stress-associated genes were confirmed by RT-PCR or RT-qPCR. Apoptotic gene expression was analyzed by RT-PCR. Before commencing SCNT, somatic cells treated with tunicamycin (TM), an ER stress inducer, confirmed the splicing of Xbp1 mRNA and increased expressions of ER stress-associated genes. In all the embryonic stages, the SCNT embryos, when compared with the IVF embryos, showed slightly increased expression of spliced Xbp1 (Xbp1s) mRNA and significantly increased expression of ER stress-associated genes (p<0.05). In all stages, apoptotic gene expression was slightly higher in the SCNT embryos, but not significantly different from that of the IVF embryos except for the Bax/Bcl2L1 ratio in the 1-cell stage (p<0.05). The result of this study indicates that excessive ER stress can be induced by the SCNT process, which induce apoptosis of SCNT embryos.

• The Journal of Korean Obstetrics and Gynecology
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• v.21 no.2
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• pp.252-262
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• 2008

Purpose: This paper is aim to report that menopausal disorder can be effectively diagnosed and treated by referring to the life event stress of patient. Methods: Researchers got a grip on the life event stress of patient by using medical examinations by interview and compared the conditions of patient before and after treatment by using Kupperman's Index. The prescription of the Gamidangguisu-san hap samhap-tang( Jiaweidangguixu-san he sanhetang) and the Gamisoyo-san (Jiaweixiaoyao-san) and the acupuncture therapy and the moxibustion therapy were used for the treatment. Results: The serious musculoskeletal systemic pains, which includes back pain, lumbago, and myalgia, and other menopausal troubles were improved remarkably. Conclusion: The menopausal disorder could be caused by not only the change of hormone but also complex factors of environmental matters which includes social cultural surroundings, mentality etc. Therefore the life event stress could be one of critical factors of menopausal disorder. The diagnosis and treatment considering the life event stress could be effective method to relieve patient's menopausal disorder.