• 한국유기농업학회지
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• 제29권4호
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• pp.589-600
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• 2021

본 연구는 산란계 육성기 홰 재질(목재 및 철재)에 따른 체중, 혈액 특성 및 깃털 손상도를 평가하기 위해 수행되었다. 산란계(Hy-Line Brown) 초생추 암컷 1,700마리를 공시하여, 두 처리구에 10반복을 두어 pen 당 85수씩 균등하게 배치하였다. 산란계 육성기간 중 체중과 균일도는 2주마다 총 10주간 조사하였으며, 혈액 특성 및 깃털 손상도 조사는 10주에 진행하였다. 산란계 육성기 10주 동안 체중 및 체중 균일도에서 목재홰와 철재홰의 유의적인 차이가 없었으며, 혈청 생화학 분석 결과도 무기성 인 조절을 제외하고 유의적인 차이를 보이지 않았다. 혈청 내 인은 목재홰가 철재홰보다 유의적으로 높은 값을 나타내었다(P<0.05). 혈구 조성(백혈구, 적혈구, HE/LY 비율)도 두 처리구간의 유의적인 차이는 없었다. 스트레스와 복지지표로 사용되고 있는 혈청 내 corticosterone 함량과 깃털 손상도는 홰 재질에 따른 유의적인 차이를 보이지 않았다. 따라서 평사 사육 시에 산란계 육성기 동안의 홰 재질은 생산성, 혈액특성, 깃털 손상도에 영향을 미치지 않았다. 본 연구 결과는 산란계 육성기 동안 홰 재질에 대한 영향을 조사하기 위한 기초 자료로 사용될 수 있을 것으로 사료된다.

• Journal of Animal Science and Technology
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• 제65권3호
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• pp.535-549
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• 2023

This study was to investigate the effects of different phytogenic feed additives (PFA) dosage levels in growing- finishing pigs stressed by high stocking density. A total of 72 mix sexed 12 weeks growing pigs ([Landrace × Yorkshire] × Duroc) with initial body weight (BW) of 49.28 ± 4.58 kg were used for 8 weeks. There were 3 replicate pens in each treatment group, with 3 pigs per pen. The dietary treatment groups consisted of basal diets in animal welfare density (negative control [NC]), basal diet in high stocking density (positive control [PC]), PC + 0.04% essential oil (ES1), PC + 0.08% essential oil (ES2), PC + 0.10% bitter citrus extract & essential oil (CES1), PC + 0.20% bitter citrus extract & essential oil (CES2), PC + 0.05% grape pomace extract (GP1), PC + 0.10% grape pomace extract (GP2). The reduction of space allowance decreased (p < 0.05) average daily gain, feed efficiency, and digestibility of dry matter, crude protein, and gross energy. Also, the fecal score of PC groups increased (p < 0.05) compared with other groups. Basic behaviors (feed intake, standing, lying) were inactive (p < 0.05) and singularity behavior (biting) was increased (p < 0.10) under high stocking density. There was no difference in blood profile. However, the supplementation of PFA alleviated the negative effects such as reduced growth performance, nutrient digestibility, and some increasing stress indicators in th blood (cortisol) and animal behavior (biting). In conclusion, the negative effect of high stocking density was most effectively mitigated by the normal dosage of the mixture of bitter citrus extract and essential oil additive (CES1).

• Journal of Animal Science and Technology
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• 제65권6호
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• pp.1308-1322
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• 2023

Minerals is required small amounts among various nutrients, but it has a significant impact on sow longevity and reproduction performance. This study was carried out to see the beneficial effects of marine-derived Ca-Mg complex on the reproductive performance of sows during four-parity periods. Seventy-two gilts ([Yorkshire × Landrace] × Duroc), with an average body weight of 181 kg, were randomly allocated to three groups; CON (basal diet), 0.3LC (CON - MgO - 0.3% limestone + 0.4% Ca-Mg complex), and 0.7LC (CON - MgO - 0.7% limestone + 0.4% Ca-Mg complex). During parity 3 and 4, the expression level of SCD gene was lower in the umbilical cord of piglets born to 0.3LC and 0.7LC sows compared with the CON sows. During parity 2, 3 and 4, SLC2A2 and FABP4 gene expressions were higher in the umbilical cord of piglets born to 0.7LC sows and the placenta of sows from 0.3LC groups, respectively. Ca-Mg complex increased (p < 0.05) Ca and Mg concentrations in sows and their piglets' serum as well as in colostrum regardless of parities. The serum vitamin D concentration was higher (p < 0.05) in their first parity, whereas serum prolactin and estrogen concentrations were higher (p < 0.05) during the fourth and third parity, respectively. The growth hormone concentrations were higher (p < 0.05) in the piglets born to sows during the first and second parity. The fat and immunoglobulin A (IgA) concentrations in colostrum were higher (p < 0.05) during the third and fourth parity, respectively. A reduction (p < 0.05) in salivary cortisol, epinephrine, and norepinephrine concentrations was observed in 0.3LC and 0.7LC sow groups compared with CON after farrowing regardless of parity, however before farrowing, a reduction in norepinephrine was observed. Before farrowing, the epinephrine and norepinephrine concentrations were higher (p < 0.05) during the first and second parity. After farrowing, the concentration of these hormones was higher during the second parity. Taken together, sows' parity and dietary Ca-Mg complex supplementation influenced serum metabolites, colostrum nutrients, stress hormones as well as the gene expressions related to lipid and glucose metabolism.

• Journal of Microbiology and Biotechnology
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• 제17권8호
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• pp.1330-1337
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• 2007

To better understand the gene expression of the cold-adapted polar diatom, we conducted a survey of the Chaetoceros neogracile transcriptome by cDNA sequencing and expression of interested cDNAs from the Antarctic diatom. A non-normalized cDNA library was constructed from the C. neogracile, and a total of 2,500 cDNAs were sequenced to generate 1,881 high-quality expressed sequence tags (ESTs) (accession numbers EL620615-EL622495). Based on their clustering, we identified 154 unique clusters comprising 342 ESTs. The remaining 1,540 ESTs did not cluster. The number of unique genes identified in the data set is thus estimated to be 1,694. Taking advantage of various tools and databases, putative functions were assigned to 939 (55.4%) of these genes. Of the remaining 540 (31.9%) unknown sequences, 215 (12.7%) appeared to be C. neogracile-specific since they lacked any significant sequence similarity to any sequence available in the public databases. C. neogracile consisted of a relatively high percentage of genes involved in metabolism, genetic information processing, cellular processes, defense or stress resistance, photosynthesis, structure, and signal transduction. From the ESTs, the expression of these putative C. neogracile genes was investigated: fucoxanthin chlorophyll (chl) a,c-binding protein (FCP), ascorbate peroxidase (ASP), and heat-shock protein 90 (HSP90). The abundance of ASP and HSP90 changed substantially in response to different culture conditions, indicating the possible regulation of these genes in C. neogracile.

• Biomolecules & Therapeutics
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• 제16권2호
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• pp.118-125
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• 2008

Gardenia jasminoides (G. jasminoides) is traditionally used to treat insomnia, jaundice, emotional disorders, hepatic disease, and inflammatory disease. Previously, we found that geniposide and the water extract of G. jasminoides increased $Cl^-$ influx in neuroblastoma. Here we examined the sychopharmacological activities of G. jasminoides and its constituents. G. jasminoides extract was orally administered at 100 and 200 mg/kg, and genipin and geniposide were intraperitoneally injected at 2, 10, and 20 mg/kg. G. jasminoides extract (200 mg/kg) significantly decreased total open field activity but increased rearing activity in the center of the open field, suggesting an increase in exploratory activity. Genipin and geniposide did not change open field activity, but geniposide (20 mg/kg) increased rearing activity in the central area. The extract (200 mg/kg) significantly decreased rotarod and wire-balancing activity, but genipin and geniposide did not. No compounds influenced thiopental-induced sleeping or electroshock-induced seizures. The extract (200 mg/kg) significantly increased staying time in the open arms of the elevated plus maze and the entry ratio into the open arms, and geniposide (20 mg/kg) also increased open arm entry. Electroshock stress decreased open arm activity, but the extract and geniposide (20 mg/kg) significantly reversed that effect. This results indicate that G. jasminoides extract and geniposide alleviated anxiety with greater efficacy in stressed animals than normal animals.

• Steel and Composite Structures
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• 제18권1호
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• pp.1-28
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• 2015

In this paper, the dynamic response of functionally gradient steel (FGS) composite cylindrical panels in steady-state thermal environments subjected to impulsive loads is investigated for the first time. FGSs composed of graded ferritic and austenitic regions together with bainite and martensite intermediate layers are analyzed. Thermo-mechanical material properties of FGS composites are predicted according to the microhardness profile of FGS composites and approximated with appropriate functions. Based on the three-dimensional theory of thermo-elasticity, the governing equations of motionare derived in spatial and time domains. These equations are solved using the hybrid Fourier series expansion-Galerkin finite element method-Newmark approach for simply supported boundary conditions. The present solution is then applied to the thermo-elastic dynamic analysis of cylindrical panels with three different arrangements of material compositions of FGSs including ${\alpha}{\beta}{\gamma}M{\gamma}$, ${\alpha}{\beta}{\gamma}{\beta}{\alpha}$ and ${\gamma}{\beta}{\alpha}{\beta}{\gamma}$ composites. Benchmark results on the displacement and stress time-histories of FGS cylindrical panels in thermal environments under various pulse loads are presented and discussed in detail. Due to the absence of similar results in the specialized literature, this paper is likely to fill a gap in the state of the art of this problem, and provide pertinent results that are instrumental in the design of FGS structures under time-dependent mechanical loadings.

• 한국통신학회논문지
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• 제39C권2호
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• pp.164-172
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• 2014

IoT(Internet of Things)로 대표되는 사물 인터넷 환경에서 최근 개인 건강 및 홈 자동화 관련 센서류 장치들이 급격히 보급되고 있다. 설치된 장치의 수가 증가함에 따라 사용자 입장에서는 이용하고자 하는 장치마다 개별 서비스 또는 어플리케이션을 찾아 실행해야 하는 번거로움이 생기고, 서비스 공급자 입장에서는 장치간의 정보 공유나 연동 및 통합 제어 기능을 제공하기 어려운 문제가 심화된다. 본 논문에서는 이러한 문제점을 해결하기 위해 댁내 인터넷 서비스의 거점이 되는 무선 인터넷 공유기를 이용, 다양한 IoT 장치들을 연동하고 통합 제어하는 방법을 제시 한다. 이를 위하여, 먼저 IoT 장치를 가상 오브젝트(Virtual Object)화하는 방법을 제시하고, 생성된 가상 오브젝트들을 무선 인터넷 공유기상에서 실행, 연동 및 관리하기 위한 서비스 구조를 제안한다. 또한 이를 직접 홈 환경의 무선 인터넷 공유기상에 구현해 다수의 IoT 장치들을 연결한 상황에서 안정적인 서비스 제공 여부와 무선 라우팅 기능과 병행 동작시의 성능에 대해 시험함으로써, 실제 환경에서의 이용 가능성을 검증한다.

• 여성건강간호학회지
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• 제26권1호
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• pp.28-36
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• 2020

Purpose: This study investigated lifestyle, depression, marital intimacy, and quality of life (QoL) in breast cancer survivors, with the goal of identifying the impacts of these factors on QoL. Methods: A sample of 146 breast cancer survivors was surveyed in this cross-sectional study. Data were collected from March 20 to May 30, 2019, using self-report structured questionnaires at a hospital located in Jeonju, Korea. Data were analyzed using the independent t-test, analysis of variance, Pearson correlation coefficients, and hierarchical regression analysis. Participants agreed to complete a face-to-face interview, including administration of the Health Promoting Lifestyle Profile II, Depression Anxiety Stress Scale 21-Depression Scale, Marital Intimacy Scale, and Functional Assessment Cancer Therapy-Breast Cancer tool. Results: QoL was positively correlated with lifestyle (r=.49, p<.001) and marital intimacy (r=.45, p<.001) and negatively correlated with depression (r=-.72, p<.001). Hierarchical multiple regression analysis showed that depression (β=-0.63, p<.001), marital intimacy (β=0.19, p=.001), and lifestyle (β=0.13, p=.031) had significant effects on the QoL of breast cancer survivors, accounting for 63.3% of variance in related QoL. Conclusion: This study provides insights into how breast cancer survivors' QoL was influenced by depression, marital intimacy, and lifestyle. To improve the QoL of breast cancer survivors, healthcare providers should consider developing strategies to decrease depression, to increase marital intimacy, and to improve lifestyle.

• Asian Pacific Journal of Cancer Prevention
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• 제15권11호
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• pp.4475-4482
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• 2014

Background: Cisplatin, a DNA damaging agent, induces apoptosis through increasing DNA fragmentation. However, identification of intrinsic resistance molecules against Cisplatin is vital to estimate the success of therapy. Bag-1 (Bcl-2-associated anthanogene) is one anti-apoptotic protein involved in drug resistance impacting on therapeutic efficiency. Elevated levels of this protein are related with increase cell proliferation rates, motility and also cancer development. For this reason, we aimed to understand the role of Bag-1 expression in Cisplatin-induced apoptosis in HeLa cervix cancer cells. Cisplatin decreased cell viability in time- and dose-dependent manner in wt and Bag-1L+HeLa cells. Although, $10{\mu}M$ Cisplatin treatment induced cell death within 24h by activating caspases in wt cells, Bag-1L stable transfection protected cells against Cisplatin treatment. To assess the potential protective role of Bag-1, we first checked the expression profile of interacting anti-apoptotic partners of Bag-1. We found that forced Bag-1L expression prevented Cisplatin-induced apoptosis through acting on Mcl-1 expression, which was reduced after Cisplatin treatment in wt HeLa cells. This mechanism was also supported by the regulation of heat shock protein (Hsp) family members, Hsp90 and Hsp40, which were involved in the regulation Bag-1 interactome including several anti-apoptotic Bcl-2 family members and c-Raf.

• 한국작물학회지
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• 제47권5호
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• pp.341-351
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• 2002

In order to examine the mechanistic basis for differential sensitivities to chilling and subsequent recovery between two rice (Oryza sativa L.) cutivars, a chilling-tolerant japonica type (Ilpumbyeo) and a chilling-susceptible indica type (Taebaekbyeo), changes of physiological responses and antioxidant enzymes were investigated. Both cultivars at 3 leaf stage were exposed at a low temperature of $5^{\circ}C$ for 3 days and subsequently recovered in a growth chamber at a $25^{\circ}C$ for 5 days with 250 mmol $m^{-2}$ $s^{-1}$. Physiological parameters such as leaf fresh weight, relative water content, cellular leakage, lipid peroxidation, and chlorophyll a fluorescence showed that the chilling tolerant cultivar had a high tolerance during chilling. However, the chilling-susceptible cultivar revealed severe chilling damages. The chilling-tolerant cultivar was also faster in recovery than the chilling-susceptible cultivar in all parameters examined. We analyzed the activity and isozyme profiles of four antioxidant enzymes which are: superoxide dismutase (SOD), caltalase (CAT), ascorbate peroxidase (APX), and glutation reductase (GR). We observed that chilling-tolerance was due to a result of the induced or higher antioxidant enzyme system, CAT and APX in leaves and SOD, CAT, APX, and GR in roots. Especially, we observed the most significant differences between the chilling-tolerant cultivar and -susceptible cultivar in CAT and APX activity. Also in isozyme profiles, CAT and APX band intensity in the chilling-tolerant cultivar was distinctively higher than in the chilling-susceptible cultivars during chilling and recovery. Thus, the cold stability of CAT and APX are expected to contribute to a tolerance mechanism of chilling in rice plants. In addition, the antioxidative enzymes activity in roots may be more important than in that of leaves to protect chilling damage on rice plants.