• Biomolecules & Therapeutics
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• v.9 no.1
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• pp.40-45
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• 2001

The activity of taurine transporter is affected by various extracellular stimuli such as ion, hormone and stress. To assess effects of steroid hormones antral cyclosporin A (CsA) on the taurine transporter activity, murine monocytic RAW264.7 cell line was stimulated with dexamethasone (DM), triamcinolone (TA), cortisone (CS), hydrocortisone (HCS), prednisone (PSN), prednisolone (PSL) and methylprednisolone (MPSL) in the presence of 12-0-tetradecanoylphorbol-13-acetate(TPA). Treatment of TPA on the cell line led to significant reduction of taurine transporter activity. However, in case of stimulation of the cells with steroid hormones in the presence of TPA, all of them recovered TPA-induced reduction of the taurine transporter activity. Treatment of the cells with CsA led to significant reduction of the taurine transporter activity. Ionomycin (IM) recovered the reduced taurine transporter activity by CsA, but failed in the presence of EDTA, a calcium chelating agent. These results showed that glucocorticoid hormone recovered TPA-induced reduction of taurine transporter activity and that IM recovered CsA-induced reduction of the transporter activity by increasing intracellular free $Ca^{++}$ concentration.n.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.7
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• pp.1031-1034
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• 1999

Circulating lymphocytes collected from control and heat-stressed buffaloes were subjected to in vitro culture with glucocorticoids, epinephrine or serotonin and their effect, if any, on the release of intracellular prolactin (PRL) was studied using ELISA and C-ELISA techniques. It was noted from the study that PRL level was higher in lymphocytes than in plasma of the control and heat-stressed animals, and that the PRL levels increased in the plasma of heat-stressed animals compared to that of non stressed animals with a significant decrease in lymphocytic PRL content by heat stress. Epinephrine and serotonin significantly increased the release of intracellular PRL from the lymphocytes of both in the control and the heat-stressed buffaloes but release of PRL from lymphocyte was not significantly changed by cortisol treatment in both control and heat-stressed buffaloes as compared to epinephrine and serotonin in vitro. When lympocytes were incubated with serotonin, it caused drastic lysis of the lymphocytes but epinephirine and cortisol did not show any lysis. It may be concluded from this study that hormones like epinephrine or serotonin known to increase during stress, release intracellular PRL from lymphocytes, the satellite PRL storage/synthesizing organ of blood, although the mechanism of the release is different.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.6
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• pp.702-707
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• 2013

Sleep is an essential biological process of which the underlying regulatory mechanisms involve numerous anatomical structures and biochemical substances that can be compromised by stress and the immune system. Gamma aminobutyric acid (GABA) is the main inhibitory neurotransmitter of the central nervous system (CNS). It is well established that activation of $GABA_A$ receptors promotes sleep. L. brevis BJ20 fermentation of sea tangle and oysters resulted in stress reduction and sleep inducing effects. This is the first study to report that GABA has the ability to induce sleep related hormones in mice; therefore, it has potential use as a natural sleep aid. These results suggested that sea tangle and oysters fermented by L. brevis BJ20 can be used as potential agents for stress reduction and sleep promotion.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.11 no.2
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• pp.203-212
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• 2004

Purpose: This study was done to determine whether providing auditory and vestibular stimulation to preform infants would have an effect on stress hormones. Methods: The design was a nonequivalent control group protest-posttest design in a quasi-experimental study Seventy-nine preform infants were assigned either one of two experimental groups or to a control group: 27 in the auditory stimulation group, 25 in the vestibular stimulation group and 27 in the control group. The criteria for inclusion in this study were 1) gestational age of less than 37 weeks, 2) birth weight of less than 2,500g, 3) the absence of congenital anomalies or specific diseases, 4) recovering physiological weight loss, and 5) weaned from ventilatory assistance or oxygen. The data were collected from March 2002 to May 2003. The auditory stimulation, a music audiotape, was provided 20 minutes twice a day for 10 days and the vestibular stimulation, an infant waterbed, was provided for 10 days. On day 1 and day 10 of the study, 24 hour urine sample was collected for norepinephrine, epinephrine, and cortisol assays. In the data analysis SPSSWIN 10.0 program was utilized for descriptive statistics, ANOVA and t-test. Results: General characteristics of the three groups showed no significant differences, thus three groups were found to be homogenous. The 24 hour urine cortisol for the auditory (t=3.489, p=.001) and for the vestibular (t=2.638, p=.013) stimulation group were significantly reduced compared to the control group after 10 days. Conclusions: The results suggest that auditory and vestibular stimulation can be used to reduce 24 hour urine cortisol in preform infants. Therefore, music audiotapes and waterbeds provided in incubator are be recommended for reduction of the stress in preform infants who are hospitalized in neonatal intensive care units.

• Journal of Animal Science and Technology
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• v.47 no.2
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• pp.253-260
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• 2005

This study was conducted to utilize the wastes produced from thinning the forest as a roughage source for ruminants. Four ruminally cannulated Korean steers were used to investigate the ruminal fermentation characteristics and blood concentrations of stress-related hormones. Treatments were composed of the rice straw only (Control) and the 30% pine silage plus 70% rice straw(Pine silage) as roughages. The experiment was conducted with four replicates by a double tum over design. For sampling of blood under high temperature steers were accommodated in respiratory chamber. Concentrations of ruminal pH, volatile fatty acids and ammonia were not affected by substitution of pine silage. Mean concentration of blood thyroxine was significantly(P < 0.01) lower in the pine silage than the control. However, blood cortisol concentration was not affected by substitution of pine silage, even though it was significantly(P < 0.05) decreased after exposure for 6 hrs in high temperature. It was concluded that the pine silage using the wastes produced from thinning the forest could be use as a substitute roughage for reduction of heat stress in ruminant.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.1
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• pp.65-67
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• 2003

Flounder (Paralichthys olivaceus) fish goes through metamorphosis in the larvae phase. In this phase, it secretes much thyroid hormone to control metamorphosis. Sharp change in the environment and nutrition deficiency may cause stress in the metamorphosis phase. It leads to interfering with the development of thyroid hormone and finally inhibits the normal growth of larvae. In this study, the correlation between the change in thyroid hormones and the albinism appearance was examined by growing them with low temperature ($13^{\circ}C$) stress over the premetamorphosis stages (stage $D\~F$) of flounder metamorphosis. Albinism rate was $15\%$ in the low temperature group and $25\%$ in the control goup. $T_{4}\;and\;T_{3}$ of the control group tended to increase from stage F as larvae grew. $T_{4}$ increased 2 times compared to the control group as 72 ng/g at stage H, the increasing phae of water temperature, in the low temperature group. $T_{3}$ increased 8 times compared to the control group as 2.9 ng/g at stage E, the decreasing phase of water temperature. Therefore, it is judged that the albinism appearance of flouner is caused from the sharp increase in $T_{3}$ by the inappropriate secretion of thyroid hormone.

• Journal of Animal Science and Technology
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• v.66 no.1
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• pp.79-92
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• 2024

Global warming is a key challenge subjecting animals to heat stress conditions resulting in multiple physiological alterations in tropical climate. Dietary approach seems to be the more friendly approach to curb the adverse effects of heat stress in rabbits. Some herbs have been categorized to have high potential for promotion of immune responses for amelioration of heat stress. Thus, this research aims to evaluate the potential of Mistletoe (Viscum album), Moringa (Moringa oleifera) and Phyllanthus (Phyllanthus amarus) leaf meal as herbal supplements for the alleviation of heat stress in female rabbits by measuring improvement in sex and stress hormonal responses in serum biochemistry. 80 Rabbit does were exposed to 4 dietary groups supplemented with each of Mistletoe, Moringa, Phyllanthus and a control in an 84-day trial at the summit of thermal stress in South west Nigeria. Growth indices were monitored throughout the study, blood samples were compiled at the end of the trial to assess serum biochemistry, stress and sex hormonal responses of the Does using standard protocols. The results revealed that final weight and weight gain of Does fed on Phyllanthus were significantly (p < 0.05) higher (11.46% and 14.25%, respectively) than Does on control. The herbal supplements enhance glucose, protein, albumin and globulin, reduced cholesterol, and creatinine of Does under heat stress conditions. Among the herbal treatment groups, mistletoe, moringa and phyllanthus had 12.42%, 18.39% and 16.90%, respectively, lower corticosterone than control groups which had 39.76ng/ml. Triiodothyronine of Does fed control were significantly (p < 0.05) lower than Does on Moringa oleifera and Phyllanthus amarus supplements. Estradiol and Follicle stimulating hormone of rabbit Does fed on moringa supplement were significantly (p < 0.05) higher than other treatments. In conclusion, the herbal supplements tend to mitigate the detrimental outcome of thermal stress on Does by suppressing stress hormones. Moringa oleifera and Phyllanthus amarus enhanced sex hormones while Phyllanthus amarus confered growth promoting effects on the Does.

• Clinical and Experimental Pediatrics
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• v.57 no.10
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• pp.425-433
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• 2014

Adrenal and thyroid hormones are essential for the regulation of intrauterine homeostasis, and for the timely differentiation and maturation of fetal organs. These hormones play complex roles during fetal life, and are believed to underlie the cellular communication that coordinates maternal-fetal interactions. They serve to modulate the functional adaptation for extrauterine life during the perinatal period. The pathophysiology of systemic vasopressor-resistant hypotension is associated with low levels of circulating cortisol, a result of immaturity of hypothalamic-pituitary-adrenal axis in preterm infants under stress. Over the past few decades, studies in preterm infants have shown abnormal clinical findings that suggest adrenal or thyroid dysfunction, yet the criteria used to diagnose adrenal insufficiency in preterm infants continue to be arbitrary. In addition, although hypothyroidism is frequently observed in extremely low gestational age infants, the benefits of thyroid hormone replacement therapy remain controversial. Screening methods for congenital hypothyroidism or congenital adrenal hyperplasia in the preterm neonate are inconclusive. Thus, further understanding of fetal and perinatal adrenal and thyroid function will provide an insight into the management of adrenal and thyroid function in the preterm infant.

• Journal of Food Hygiene and Safety
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• v.15 no.3
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• pp.199-203
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• 2000

With a rabbit model, the present study was performed to examine the effect of Escherichia coli lipopolysaccharide (E. coli LPS) on hormones. Cortisol, epinephrine, and norepinephrine concentrations in LPS-treated groups were high at all sampling periods (from 3 hrs to 72 hrs) as compared to control group (p<0.05 or p<0.01). The each peak time was at respectively 24 hrs, 3 hrs, and 12 hrs. Insulin and glucagon concentrations in LPS-treated groups elevated up to 12 hrs (p<0.01 or p<0.05) with a each peak point at 12 hrs or 6 ks, while those of the rest sampling points (from 24 hrs to 72 hrs) were lower than that of control (p<0.05). Increase of cortisol concentration was generally dose-dependent, whereas the changes of the other hormones were irregular patterns. These observations show that E. coli LPS lead to releases of stress hormones such as cortisol, epinephrine, and norepinephrine and disturbances of endocrine systems. These LPS-induced hormonal disorders may cause physiologically fatal results.

• Korean Journal of Exercise Nutrition
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• v.24 no.3
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• pp.25-31
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• 2020

[Purpose] Epidemiological evidence has shown that leisure-time physical activity and structured exercise before and after breast cancer diagnosis contribute to reducing the risk of breast cancer recurrence and mortality. Thus, in this review, we aimed to summarize the physical activity-dependent regulation of systemic factors to understand the biological and molecular mechanisms involved in the initiation, progression, and survival of breast cancer. [Methods] We systematically reviewed the studies on 1) the relationship between physical activity and the risk of breast cancer, and 2) various systemic factors induced by physical activity and exercise that are potentially linked to breast cancer outcomes. To perform this literature review, PubMed database was searched using the terms "Physical activity OR exercise" and "breast cancer", until August 5th, 2020; then, we reviewed those articles related to biological mechanisms after examining the resulting search list. [Results] There is strong evidence that physical activity reduces the risk of breast cancer, and the protective effect of physical activity on breast cancer has been achieved by long-term regulation of various circulatory factors, such as sex hormones, metabolic hormones, inflammatory factors, adipokines, and myokines. In addition, physical activity substantially alters wholebody homeostasis by affecting numerous other factors, including plasma metabolites, reactive oxygen species, and microRNAs as well as exosomes and gut microbiota profile, and thereby every cell and organ in the whole body might be ultimately affected by the biological perturbation induced by physical activity and exercise. [Conclusion] The understanding of integrative mechanisms will enhance how physical activity can ultimately influence the risk and prognosis of various cancers, including breast cancer. Furthermore, physical activity could be considered an efficacious non-pharmacological therapy, and the promotion of physical activity is probably an effective strategy in primary cancer prevention.