• Title/Summary/Keyword: Streptozotocin-diabetic

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Effects of Coated Liposome from Discorea rhizoma Extract (DRE) -on Hypoglycemic, Serum Insulin, and Lipid Levels in Streptozotocin-Induced (산약 추출물의 리포좀 처리가 Streptozotocin으로 유발된 당뇨 마우스의 혈당 강하 효과와 혈장 인슐린 및 지질에 미치는 영향)

  • Choi, Kyung-Soon
    • The Korean Journal of Food And Nutrition
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    • v.26 no.2
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    • pp.310-317
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    • 2013
  • To investigate the effects of coated liposome from Discorea rhizoma extract (DRE) in streptozotocin (STZ)-induced, we evaluated changes in body weight, fasting blood glucose, blood insulin and blood lipid concentrations in mice. Mice were divided into four groups: (DC), diabetic DRE at 10 mg/kg (DRE-1), diabetic DRE at 50 mg/kg (DRE-2), and diabetic DRE at 250 mg/kg (DRE-3). Mice had free access to water and diet (10 weeks). The DC group showed higher blood cholesterol than the DRE-1, DRE-2, DRE-3 groups. In glucose tolerance test, the DRE-1, DRE-2, and DRE-3 groups increased after 30 minutes in decremental glycemic response area under the curve. Fasting blood glucose levels in the DRE groups significantly decreased through 4 weeks. Plasma total cholesterol, triglyceride and LDL-cholesterol concentrations were also lower in the DRE groups. On the other hand, the DRE-1, DRE-2 and DRE-3 groups showed higher HDL-cholesterol and insulin levels than the DC group. Moreover, blood glucose and lipid levels significantly decreased in streptozotocin (STZ)-induced diabetic mice treated with DRE. These results indicate that DRE may reduce elevated blood glucose levels and serum lipid concentrations in hypoglycemic and diabetic mice, suggesting its usefulness as a functional food.

Effect of Coicis Semen Extract on Streptozotocin-Induced Diabetic Nephrophthy Rats (의이인(薏苡仁) 추출물의 경구투여가 Streptozotocin에 의한 흰쥐의 당뇨병성 신증에 미치는 영향)

  • Kim, Hyung-Woo;Kim, Jung-Sang;Cho, Su-In
    • The Korea Journal of Herbology
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    • v.21 no.3
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    • pp.75-81
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    • 2006
  • Objectives : The present study was carried out to investigate the beneficial effect of Coicis Semen extract(CSe) on streptozotocin(STZ)-induced diabetic nephropathy rats. CSe was given to rats with oral administration. Methods : The experimental animals were divided into 3 groups : normal group of rats, control group of STZ-induced diabetic rats, sample group with CSe treatment. Experimental diabetes was induced by the injection of STZ(60 mg/kg) to the rat via the peritoneum. The effects of CSe on STZ-induced diabetic nephropathy were observed by measuring the serum level of creatinine, BUN and uric level of glucose. Kidney level of lipid peroxidation and the activities of reduced glutathione(GSH) were also examined Results : STZ-induced increase of serum creatinine was lowered by CSe treatment, but BUN and uric level of glucose did not show significant changes. CSe oral administration showed statistical decrease of lipid peroxidation in renal cortical tissues, but it has no effect on the activities of GSH. Conclusion : CSe treatment showed protective effect on rat diabetic nephropathy model, but action mechanism of the effect was still not dear. We thought to be concerned with anti-oxidative stress.

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Hypoglycemic Activity of Opuntia ficus-indica var.sabotan on Alloxan- or Streptozotocin-Induced Diabetic Mice (Alloxan 및 Streptozotocin 유도 당뇨모델 동물에서 손바닥 선인장의 혈당강하 효과)

  • Shin, Ji-Eun;Han, Myung-Joo;Lee, In-Kyung;Moon, Young-In;Kim, Dong-Hyun
    • Korean Journal of Pharmacognosy
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    • v.34 no.1 s.132
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    • pp.75-79
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    • 2003
  • Hypoglycemic activity of Opuntia ficus-indica var. sabotan on alloxan or streptozotocin-induced diabetic mice was investigated. Fructus and folium of Opuntia ficus-indica vars. sabotan inhibited intestinal ${\alpha}-glucosidase$ of rats as well as glucose elevation in blood of normal mice, Opuntia ficus-indica var. sabotan exhibited inhibitory activities on streptozotocin-induced diabetic mice rather than on alloxan-induced diabetic mice loaded with maltose and sucrose. Its folium was more effective than its fructus. These results suggest that Opuntia ficus-indica var. sabotan might be effective on diabetic mellitus.

Antidiabetic Effect of Ethanol Extract of Lacca Sinica Exsiccata on Streptozotocin-induced Diabetic Rats (건칠 추출물이 Streptozotocin으로 당뇨를 유도한 흰쥐에 미치는 영향)

  • Oh, Hyun-Joo;Ko, Seong-Gyu;Shin, Yong-Cheol
    • Journal of Society of Preventive Korean Medicine
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    • v.10 no.1
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    • pp.75-93
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    • 2006
  • Rhus verniciflua Stokes(RVS) has been widely used as a food and traditional herbal medicine in Korea. RVS has been reported that the extract from its wood and fruit has strong antioxidant activity and anticancer effect but there is little information on Lacca Sinica Exsiccata(LSE), the resin of RVS, as a medicinal use. The aim of this study was to evaluate the antidiabetic effect of ethanol-eluted extract of LSE on streptozotocin(STZ) - induced diabetic rats. Diabetes was induced in male Sprague-Dawley rats with STZ injection. Oral administration of LSE extract(50mg or 100mg/kg of body weight/day) was given to diabetic group. During 4 weeks of experiment, diabetic rats showed significant weight loss and decreasing feed efficiency ratios(FER) compared with normal rats, while the diabetic group orally fed with LSE extract showed a trend of decreasing weight loss and a significant increase of FER(p<0.05). In 4 weeks after induction of diabetes, diabetic rats showed an increase in weight of liver, kidney and heart, whereas the diabetic rats administered with LSE extract showed a reduction in the weight of heart. Blood glucose level was decreased in diabetic rats treated with LSE extract, but it was not statistically significant. Glutamic oxaloacetic transaminase, Glutamic pyruvate transaminase and total cholesterol levels were lower in the diabetic group treated with LSE extract than in untreated diabetic group, but not significant. These results present that LSE may partly have antidiabetic effect and may protect against the development of diabetic heart complications resulting from impaired glucose metabolism.

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Effects of Iksujisundan on Renal Function, Peroxynitrite Scavenging Activity and Polyol Pathway in Streptozotocin-induced Diabetic Rats (익수지선단(益壽地仙丹)이 Streptozotocin으로 유발된 당뇨병 흰쥐의 신기능, 활성산소, 활성질소 및 Polyol Pathway에 미치는 영향)

  • Jeon, Chang-Min;Jeong, Ji-Cheon
    • The Journal of Korean Medicine
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    • v.28 no.1 s.69
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    • pp.237-248
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    • 2007
  • Objectives : Diabetes is a disease in which the body does not produce or properly use insulin. Etiological studies of diabetes and its complications showed that oxidative stress might play a major role. Therefore, many efforts have been tried to regulate free oxygen radicals for treating diabetes and its complications. Iksujisundan has been known to be effective for the treatment of diabetes. The present study was carried out to investigate the effect of Iksujisundan on renal function, peroxynitrite(ONOO-) scavenging activity and polyol pathway in streptozotocin-induced diabetic rats. Methods : The crushed Iksujisundan was extracted 3 times, each time with 3 volumes of methyl alcohol at 60$^{\circ}C$ for 24 h. The extract was filtered and evaporated under a reduced pressure using a rotary evaporator to yield 87.8g. Iksujisundan extract was orally administreted at 100 mg per 1 kg of body weight for 20 days to the diabetic rats induced by streptozotocin(60mg/kg). The effects of Iksujisundan extract on the streptozotocin-induced diabetic rats were observed by measuring the serum level of glucose, insulin, lipid components, creatinine and BUN, and also the kidney levels of superoxide anion radical(${\cdot}$O2-), nitric oxide(NO) and ONOO-, and also the enzyme activities involved in the polyol pathway. Results : The effects of Iksujisundan on the streptozotocin-induced diabetic rats with regards to body weight, blood glucose and indulin levels, creatinine and BUN levels, total cholesterol and triglyceride lavels, and HDL-cholesterol levels were all shown to be good enough to prevent and cure the diabetes and its complications. Iksujisundan inhibited the generation of ${\cdot}$O2-,NO and ONOO- in the kidney of streptozotocin-induced diabetic rats. Renal aldose reductase and sorbitol dehydrogenase activities were increased in the streptozotocin-induced diabetic rats were reversed toward natural activities. Conclusions : Iksujisundan might inhibit the development of diabetes and its complications by scavenging reactive oxygen and nitrogen species, thereby by reducing oxidative stresses and also by regulating the activities of polyol pathway enzymes, all of which could help to recover kidney function.

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Antidiabetic Effect of Glechoma longituba (Nakai) Kupr in Diabetic Rats induced by Streptozotocin (연전초(連錢草) 추출물이 Streptozotocin으로 유발시킨 흰쥐의 당뇨에 미치는 영향)

  • Lee, Kyung-Jin;Ham, In-Hye;Bu, Young-Min;Kim, Ho-Cheol;Choi, Ho-Young
    • The Korea Journal of Herbology
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    • v.23 no.3
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    • pp.175-180
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    • 2008
  • Objecitivies : While Glechoma longituba (Nakai) Kupr(GL) has been known as a antiinflammatory, antidiabetic and antihypertension medicine in korea traditional medicine, there has been only a few researches about it in Korea. Thus, we examined antidiabetic effect of GL in diabetic rats induced by streptozotocin Methods : To verify the effects of GL on serum glucose, creatinine, BUN, total cholesterol and triglyceride through streptozotocin-induced diabetic rats, some rats were injected 1 time with 55mg/kg streptozotocin intraperitoneally to induce diabetes, and other rats were injected with buffer I.P. as control group. Results : GL extracts significantly reduced the amount of serum glucose, BUN, creatinine, and triglyceride level in diabetics rats induced by streptozotocin. Conclusions : According to above result, Glechoma longituba was expected to be applied to the treatment of diabetes mellitus.

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Effect of the Lycii Fructus on multiple low-dose streptozotocin-induced diabetic rats (Streptozotocin 저용량 반복투여로 유도한 당뇨 흰쥐에서 구기자 추출물의 효능연구)

  • Oh, Tae Woo;Park, Yong-Ki
    • The Korea Journal of Herbology
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    • v.30 no.6
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    • pp.47-53
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    • 2015
  • Objectives : This study was performed to investigate the anti-diabetic effect of the fruit extract of Lycium chinense Mill(Lycii Fructus, LF) on multiple low-dose streptozotocin-induced diabetic rats.Methods : Male Sprague-Dawley rats were divided into three groups; normal, STZ-control, Lycii Fructus extractorally administrated 300 ㎎/㎏ group (STZ-LF). Diabetes was induced in rats by consecutive injection of streptozotocin (STZ) at doses of 30 ㎎/㎏ for 5 days. After 4 weeks, all rats were sacrificed, Fasting blood glucose, total cholesterol (CHO), triglyceride (TG) and HDL-Cholesterol were measured in sera of rats. Histopathological changes of pancreas, kidney, liver and lung tissues were observed by microscope after H&E, Periodic acid-Schiff (PAS) and Masson's trichrome staining. The changes of body weight, blood glucose, and food and water intake were also measured.Results : There were no differences in body, food intake and water intake in LF-administrated groups compared with STZ control group. However, LF extract significantly decreased the levels of serum glucose, CHO, TG and HDL-Cholesterol in diabetic rats. In histopathological analysis of kidney, liver and lung, LF-administrated groups showed the inhibition of morphological damage.Conclusions : These results suggest that LF have a biological action on multi low-dose STZ-induced diabetes in rats via decreasing the serum glucose, TG and TG levels and may protect the morphological changes of kidney, liver and lung.

Effects of Indongdeung Jigolpi-tang applied to Soyangin on Hyperglycemic Rats induced by Streptozotocin (소양인(少陽人)에 응용(應用)되는 인동등지골피탕(認冬藤地骨皮湯)이 Streptozotocin 투여(投與) 고혈당(高血糖) 백서(白鼠)에 미치는 영향(影響))

  • Ko, Ki Duk;Hong, Sun-Yong
    • Journal of Sasang Constitutional Medicine
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    • v.2 no.1
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    • pp.149-166
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    • 1990
  • In order to investigate experimented effects of Indongdeung Jigolpi-Tang, which is applied to Soyangin, to diabetes, the measurement, comparison and observation made on the contents of volumes of blood glucose, total cholesterol, triglyceride, phospholipid, total protein and electrolyte($K^+$, $Na^+$) in blood serum by giving a high blood glucose, induced by streptozotocin, to hyperglycemic rats, dosing the cooked liquid of I.J.T., and following conclusion was attained. 1. The volume of glucose urine, induced by streptozotocin to hyperglycemic rats, was shown an increase in comparision to a normal flock when $12.50m{\ell}/kg$ of I.J.T. dosed to the rats and checked after two and four weeks and thereby, a sinificant reduction in the volume of blood glucose was made. 2. The volume of total cholesterol of blood serum in the diabetic rats, induced by streptozotocin, was increased in comparision to the normal flock, and in the fouth week of dosing $1.25m{\ell}/kg$ of I.J.T., a significant reduction of total cholesterol volum was shown in the blood serum. 3. The volume of triglyceride in the blood serum of the diabetic rats, induced by the streptozotocin, was increased in comparision to the normal flock, and with dosing $1.25m{\ell}/kg$ and $12.50m{\ell}/kg$ of I.J.T., a significant reduction of triglyceride volium was shwon in the blood serum after second and fourth week. 4. The volume of phospholipid in the blood serum of the diabetic rats, induced by the streptozotocin, was somewhat reduced in comparision to the normal flock, and with dosing $1.25m{\ell}/kg$ of I.J.T., a significant reduction of phospholipid volium in the blood serum was shown after first and second week. 5. The volume of total protein in the blood serum of the diabetic rats, induced by the streptozotocin, was somewhat reduced in comparison to the normal flock however, with dosing the I.J.T., no significant change on the volium of total protein was made in the blood serum. 6. The volume of potassium in the blood serum of the diabetic rats, induced by the streptozotocin, was increased in comparision to the normal flock however, with dosing the I.J.T., no significant change of potassium content was made in the blood serum. 7. The volume of sodium in the blood serum of the diabetic rats, induced by the streptozotocin, was somewhat reduced in comparision to the normal flock, and with dosing $12.50m{\ell}/kg$ of the I.J.T., a significant increase was shown in the blood serum after first week. Soyangin, accompanying with a middle and upper heat, the I.J.T. - that has a merits of cleaning up fever, solving fever, solving poison, solving thirst and restoring exhaustion - can be proved that it has a blood sugar reduction effect and it is considered that it can be meritorious for strengthening vitality, further, many other exhausting diseases, and can actively used for the cases such as thirst causing disease and a disease that causes an abnormal food hunger, thirst causing and frequent going to pass urine, in particular.

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Anti-diabetic Effect of Indongdeungjikolpi-tang in Streptozotocin-induced Diabetic Rats (인동등지골피탕(忍冬藤地骨皮湯)이 Streptozotocin으로 유발된 흰쥐에서의 항당뇨 효과에 대한 연구)

  • Bae, Hyo-Sang;Park, Seong-Sik;Jung, Jin-Ki;Yoon, Cheol-Ho;Byun, Sang-Hyuk;Park, Yong-Ki
    • The Korea Journal of Herbology
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    • v.23 no.4
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    • pp.103-112
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    • 2008
  • Objectives: Indongdeungjikolpi-tang(IJT) is used as a traditional treatment of diabetes in oriental clinincs. This study aimed to evaluate the anti-diabetic effect of Indongdeungjikolpi-tang(IJT) in streptozotocin(STZ)-induced diabetic rats. Methods: Diabetes was induced by intraperitoneal injection of streptozotocin(STZ; 60 mg/kg BW) to Sprague-Dawley male rats. Experimental animals(six per group), were treated by oral administration of IJT(100 and 500 mg/kg BW) and glibendimide(3 mg/kg), a known antidiabetic drug for comparison, during 4 weeks. We measured the levels of glucose, insuline, triglyceride, creatinine and urea in sera of each group. An oral glucose tolerance test(OGTT) was also performed in all groups. Results: IJT(100 and 500 mg/kg) significantly reduced blood glucose levels and increased plasma insulin levels in STZ-induced diabetic rats. IJT also significantly reduced the plasma levels of tryglyceride, creatinine and urea in STZ-induced diabetic rats. The OGTT results showed a significant improvement in glucose tolerance in IJT-administrated rats. Conclusions: These data indicate that IJT may improve glocose homeostasis in STZ-induced diabetes, which could be associated with stimulation of insulin secretion.

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The Effects of Nicotinamide on the Serum Lipid Composition in Streptozotocin-Induced Diabetic Rats (Nicotinamide가 Streptozotocin 당뇨성 쥐의 혈중 지질 성분에 미치는 영향)

  • 최종원;손기호;김석환
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.4
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    • pp.306-311
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    • 1991
  • The purpose of this study was to investigate the effect of pretreatment with nicotinamide on the serum lipid composition and atherosclerotic index in streptozotocin ( STZ ) - induced diabetic rats. Nicotinamide pretreatment in STZ-induced diabetic rats inhibited the rise of serum glucose concentration. Serum total lipids and triglyceride levels in the STZ-induced diabetic rats were significantly higher than those in the control group. But in the group pretreated with nicotin-amide, triglyceride and lipid levels were significantly lower compared with those of STZ-induced diabetic rat group without nicotinamide. However, the serum phospholipid levels were not statistically different among treatment groups. In the STZ-induced diabetic group, the serum total cholesterol, VLDL, LDL-cholesterol levels and atherosclerotic index were higher and HDL-cholesterol level was lower compared to the control group. However, these changes were prevented by nicotinamide pretreatment. Pretreatment with nicotinamide significantly increased tile activities of serum lipase compared to the STZ-treated group. Aminotranferase (ALT, AST) activities were not significantly different in any of the groups.

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