• 대한본초학회지
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• 제23권3호
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• pp.85-91
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• 2008

Objectives : This study aimed to evaluate the anti-diabetic effect of Wen-Pi-Tang-Hab-Wu-Ling-San (WHW) extract in streptozotocin(STZ)-induced type-1 diabetic rats. Methods : Experimental diabetes were induced by intraperitoneal injection of streptozotocin (60 mg/kg). Two groups of STZ-induced diabetic rats were given the following treatments for 2 weeks by oral Administrations : (1) WHW 10 mg/kg, (2) WHW 100 mg/kg. In addition, vehicle-treated diabetic and nondiabetic controls were used in the experiment. The effects of WHW extract on STZ-induced diabetes were observed by measuring the changes of body weights and the levels of fasting blood glucose, insulin, urea nitrogen (BUN) and creatinine level in sera of rats, respectively. Results : In comparison control group, WHW-treated groups (100 mg/kg) were significantly decreased fasting blood glucose levels and increased serum insulin levels in STZ-induced diabetic rats. Moreover, WHW-treated groups (100 mg/kg) were reduced s-creatinie levels in STZ-induced diabetic rats. In addition, the changes related to diabetic nephropathy with body weight were significantly lower in WHW extract-dosing groups than in the diabetic control. Conclusions : The study thus showed that WHW extract enhanced the anti-diabetic effect in STZ-induced diabetic rats by improving the hypoglycemia. It also increased pancreatic insulin content in these rats.

• Journal of Nutrition and Health
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• 제36권10호
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• pp.981-989
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• 2003

The hypoglycemic effects of four edible plants (Angelicae tenuissimae (A. ten.), Pleurospermum kamtschaticum (P. kam.), Adenophora remotiflora (A. rem.) and Zanthoxylum schinifolium (Z. sch.)) in streptozotocin (STZ) -induced diabetic rats were investigated. Sprague-Dawley male rats weighing 190-230 g were induced diabetes mellitus by the STZ injection (45 mg/kg) into the tail vein and were divided into six groups ; normal, STZ-control and four edible plant groups (A. ten., P kam., A. rem. and Z. sch. groups). Normal and STZ-control groups were fed a AIN-93 diet and four groups of STZ-induced diabetic rats were fed one of each experimental diets containing 10％ of the edible plant powder for 4 weeks. Diabetic rats showed the lower weight gain compared to the normal rats. In experimental groups except P. kam., AST activities were close to normal. A. ten. group were lowered ALT activities slightly. The plasma glucose levels of the diabetic experimental groups were significantly decreased at 4th week. The plasma insulin levels in diabetic experimental groups were not significantly different compared to the STZ-control group. The liver glycogen levels in STZ injected rats were significantly lower in compared to the normal rats. However no significant differences were found in response experimental plants intake in diabetic rats. The muscle glycogen were not significantly different among all the groups.

• 한국식품영양과학회지
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• 제20권4호
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• pp.306-311
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• 1991

Streptozotocin(STZ)에 의해서 유도된 당뇨성 쥐에서 nicotinamide의 영향을 알아보기 위하여 혈중 당의농도, 지질함량 변동과 동맥경화 지표와의 상관관계를 관찰하였다. STZ 투여로서 혈중당의 농도가 증가되던 것이 nicotinamide의 전처리로 정상상태로 억제되었고 total lipid 및 triglyceride 함량에서도 STZ 투여로 증가되던 것이 nicotinamide 전처리로 억제되었으나 phospholipid 함량에는 별다른 영향이 없었다. 혈중 total cholesterol. VLDL, LDL-cholesterol 함량 및 동맥경화 지표는 STZ에 의해 증가되었으나 nicotinamide 전처리로 저지되었으며 HDL-cholesterol 함량에서는 STZ 투여에 의해 감소되던 것이 nicotinamide의 전처리로 회복되었다. 혈중 lipase의 활성은 STZ 투여로서 억제되던 것이 nicotinamide의 전처리로 회복되었다. s-ALT 및 s-AST의 활성은 STZ 투여군이나 nicotinamide 전처리 군에서나 별다른 영향이 없었다. 이상의 실험 결과에서 nicotinamide의 전처리는 STZ에 의하여 유도되는 당뇨병을 예방할 것이라는 사실은 다른 측면에서 뒷받침하는 것으로 본 연구에서는 당뇨성 고지혈증의 예방에 nicotinamide가 유용하게 응용될 수 있는 가능성을 제시하고 있다.

• Journal of Acupuncture Research
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• 제25권1호
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• pp.57-72
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• 2008

Objectives : The Herb-combined Remedy(HCR) for diabetes mellitus is known as an anti-hyperglycaemic agent. But its exact mechanisms are unclear. The present study was carried out to investigate its anti-hyperglycaemic and anti-oxidative effects in STZ-induced diabetic rats. Methods : Experimental diabetes was induced by injection of STZ(80mg/kg) to ratsvia the peritoneum. The experimental animals were divided into 4 groups : normal group, control group(STZ-induced diabetic rats with no treatment), HCR group(STZ-induced diabetic rats with HCR treatment), MF group(STZ-induced diabetic rats with Metformin treatment). The effects of HCR on STZ-induced diabetes was observed by measuring fasting blood glucose, changes of body weight, food uptake, and water uptake glucose levels in the normal state decline rates in blood glucose levels DPPH free-radical scavenging activity superoxide dismutase in RBC lysate catalase activity in RBC lysate and glutathione reductase activity in RBC lysate. Results : Treatment with HCR regulated blood glucose levels. Treatment with HCR also prevented weight loss in STZ-induced diabetic rats. In addition, oral glucose tolerance decreased following treatment with HCR. Direct anti-oxidative effects on DPPH free-radical scavenging were not observed, but treatment with HCR elevated SOD levels in blood cell lysates from STZ-induced diabetic rats. In addition, the HCR-treatment group showed an elevated tendency to glutathione reductase activity. Conclusions : These results demonstrate that HCR has anti-hyperglycaemic and anti-oxidative effects in STZ-induced diabetic rats.

• 한국조리학회지
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• 제13권3호
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• pp.199-206
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• 2007

Hypoglycemic efficacy of natural functional mixture(FM) and level of the diabete related hormones in streptozotocin(STZ)-induced diabetic rats were investigated. Male Sprague-Dawley rats were divided into three groups (normal, diabetic fed diets with/without FM). Supplement of FM did not affect the body weight and feed intake of STZ-induced diabetic rats. The increase in the weight of liver of STZ-induced diabetic rats was weakened by supplement of FM, whereas the weight of kidney and heart was not affected. Blood glucose level was slightly, and glucose tolerance of post-feeding was significantly improved by functional mixture. The mixture significantly reduced the elevated HbA1C level of diabetic rats by 15%, and it increased the level of insulin and C-peptide in blood and decreased glucagon level. Therefore, we conclude that FM in this study has a potency of prevention and treatment of diabetes mellitus.

• 생명과학회지
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• 제20권5호
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• pp.691-696
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• 2010

본 연구에서는 STZ로 당뇨병이 유발된 흰쥐의 간, 신장, 췌장 조직으로부터 소당탕(SDT)의 항산화 효과를 확인하여 다음과 같은 결과를 얻었다. 1. SDT의 투여는 간, 신장, 췌장 내 항산화효소인 SOD의 활성을 유의적으로 증가시켰다. 2. SDT의 투여는 간, 신장, 췌장 내 항산화물질인 GSH의 농도를 유의적으로 증가시켰다. 3. SDT의 투여는 간, 신장, 췌장 내 지질과산화 산물인 MDA의 농도를 유의적으로 감소시켰다. 이상의 결과로 볼 때 소당탕은 STZ로 유발된 당뇨병 흰쥐에서 항산화효소 활성과 항산화물질 생성을 증가시키고, 산화물질 생성을 억제함으로써 항산화 효과가 확인되었다.

• 대한본초학회지
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• 제21권3호
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• pp.75-81
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• 2006

Objectives : The present study was carried out to investigate the beneficial effect of Coicis Semen extract(CSe) on streptozotocin(STZ)-induced diabetic nephropathy rats. CSe was given to rats with oral administration. Methods : The experimental animals were divided into 3 groups : normal group of rats, control group of STZ-induced diabetic rats, sample group with CSe treatment. Experimental diabetes was induced by the injection of STZ(60 mg/kg) to the rat via the peritoneum. The effects of CSe on STZ-induced diabetic nephropathy were observed by measuring the serum level of creatinine, BUN and uric level of glucose. Kidney level of lipid peroxidation and the activities of reduced glutathione(GSH) were also examined Results : STZ-induced increase of serum creatinine was lowered by CSe treatment, but BUN and uric level of glucose did not show significant changes. CSe oral administration showed statistical decrease of lipid peroxidation in renal cortical tissues, but it has no effect on the activities of GSH. Conclusion : CSe treatment showed protective effect on rat diabetic nephropathy model, but action mechanism of the effect was still not dear. We thought to be concerned with anti-oxidative stress.

• 생약학회지
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• 제40권3호
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• pp.213-217
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• 2009

This study was done to investigate the antioxidative effect of polygoni radix in Streptozotocin (STZ)-induced diabetic rats. Diabetes was induced by intravenous injection of STZ at a dose of 45 mg/kg dissolved in citrate buffer. The ethanol extract of Polygoni radix was orally administrated once a day for 7 days. The contents of Total cholesterol, triglyceride (TG) were significantly decreased in Polygoni radix treated STZ-sample group compared with to the those of STZ-control group. The hepatic cytosolic activities of glutathione-s-transferase (GST), superoxide dismutase (SOD) were significantly increased, Also the content of glutathione (GSH) was incresaed in Polygoni radix treated STZ-sample group compared with to the those of STZ-control group. but the content of malondialdehyde (MDA) and the hepatic cytosolic activity of Catalase (CAT) were decreaed but not statistical significant. These results indicated that ethanol extract of polygoni radix was effective for the antidxidative in the STZ-induced diabetic rats.

• 대한본초학회지
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• 제30권6호
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• pp.47-53
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• 2015

Objectives : This study was performed to investigate the anti-diabetic effect of the fruit extract of Lycium chinense Mill(Lycii Fructus, LF) on multiple low-dose streptozotocin-induced diabetic rats.Methods : Male Sprague-Dawley rats were divided into three groups; normal, STZ-control, Lycii Fructus extractorally administrated 300 ㎎/㎏ group (STZ-LF). Diabetes was induced in rats by consecutive injection of streptozotocin (STZ) at doses of 30 ㎎/㎏ for 5 days. After 4 weeks, all rats were sacrificed, Fasting blood glucose, total cholesterol (CHO), triglyceride (TG) and HDL-Cholesterol were measured in sera of rats. Histopathological changes of pancreas, kidney, liver and lung tissues were observed by microscope after H&E, Periodic acid-Schiff (PAS) and Masson's trichrome staining. The changes of body weight, blood glucose, and food and water intake were also measured.Results : There were no differences in body, food intake and water intake in LF-administrated groups compared with STZ control group. However, LF extract significantly decreased the levels of serum glucose, CHO, TG and HDL-Cholesterol in diabetic rats. In histopathological analysis of kidney, liver and lung, LF-administrated groups showed the inhibition of morphological damage.Conclusions : These results suggest that LF have a biological action on multi low-dose STZ-induced diabetes in rats via decreasing the serum glucose, TG and TG levels and may protect the morphological changes of kidney, liver and lung.

• Journal of Community Nutrition
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• 제2권2호
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• pp.164-169
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• 2000

The purpose of this study was to examine the effect of taurine supplementation time on the activity of the enzymes metabolizing lipid peroxide in the liver of streptozotocin(STZ)-induced diabetic rats, Sprague-Dawley male rats were fed the purified diet for 7 weeks. They were supplemented with or without 1% taurine in drinking water before or after STZ injection or during all experimental procedure. In comparison to diabetic group without taurine, glucose-6-phosphatase(G6Pase) activity was decreased in diabetic group supplemented with taurine before STZ injection, and it was increased in diabetic group supplemented with taurine after STZ injection, but the difference was not significant. Glutathione S-transferase(GST) activity was significantly increased by the injection of STZ. However, the GST activities of diabetic groups exposed to taurine after STZ injection or during all experimental procedure were significantly decreased. Glutathione peroxidase(GPx) activities was significantly decreased by STZ injection. However, only in diabetic group supplemented with taurine before STZ injection, GPx activities was not decreased by the STZ injection. These results suggest that taurine supplementation may change the activities of GSH-related enzymes metabolizing lipid peroxide in the liver of streptozotocin(STZ)-induced diabetic rats and that may be helpful for the prevention of diabetic complication.