• Title/Summary/Keyword: Streptococcus

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Antimicrobial Resistance Patterns of Vancomycin-Resistant Streptococcus equinus Isolated from Animal Foods and Epidemiological Typing of Resistant S. equinus by Microbial Uniprimer Kit

  • Choi, Sung-Sook;Lee, Jin-Woo;Kang, Byoung-Yong;Ha, Nam-Joo
    • Archives of Pharmacal Research
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    • v.26 no.8
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    • pp.638-643
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    • 2003
  • Raw milk samples, and cow and chicken intestines were tested to isolate vancomycin-resistant, gram-positive bacteria. From these samples, we isolated seven vancomycin-resistant Streptococcus equinus, two vancomycin-resistant viridans Streptococcus and two vancomycin-resistant Enterococcus faecium. The MICs of several antibiotics, including vancomycin, against these strains were tested. Seven isolates of S. equinus showed high level resistance to vancomycin and teicoplanin (>100 $\mu$ g/mL). The cell wall thickness of these strains was compared with that of the sensitive strain by TEM and no differences were obserbed between these strains. We compared the strains of vancomycin-resistant Streptococcus equinus using PCR with Microbial Uniprimer Kit. We concluded that it is necessary to combine other methods in order to cluster and identify all isolates of S. equinus.

Effects of Zinc Containing Solution on Oral Microorganisms (Zinc 수용액이 구강 미생물에 미치는 영향)

  • 이상구;김은숙;이승우
    • Journal of Oral Medicine and Pain
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    • v.23 no.4
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    • pp.343-352
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    • 1998
  • This experiment was performed to investigate effects of zinc containing solution on the major normal flora Staphylococccus aureus, Streptococus mutans and Candida albicans and to observe the variation according to anionic change and concentration difference. Zinc chloride, zinc iodide and zinc acetate solution were added to werially diluted broth culture so that each final concentration might be 0.25%, 0.5%. 1%. After that, 100ul of each aliquot was spreaded on each selective media plate( Mannitol Salts Agar plate for Staphylococcus aureus, Mitis Salivarius Agar plate for Streptococcus mutans and Sabouraud Destrose Agar plate for Candida albicans). The % killing was calculated bu CFU count after incubation under the appropriate condition. 1. zinc iodide, zinc chloride, and zinc acetate solutions showed inhibitory effects on Staphylococcus aureus, Streptococcus mutans and Candida albicans. 2. The inhibitory effects on Staphylococcus aureus were ranked in order of ainc iodide, zinc chloride and zinc actate. 3. The inhibitory effects on Streptococcus mutans were ranked in orfer of zinc iodide, zinc chloride and zinc acetate. 4. the inhibitory effects on Candida albicans showed no difference among zinc iodide, zinc chloride and zinc acetate. 5. The inhibitory effects of zinc chloride and zinc acetate on Staphylococcus aureus and Streptococcus mutnas showed increasing pattern as the concentration increase. But the inhibitory effects of zinc iodide on Staphylococcus aureus and Streptococcus mutans showed no apparent difference according to concentrations and it was the case with the inhibitory effects of zinc iodide, zinc chloride and zinc acetate on Candida albicans.

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Phylogenetic Diversity and Comparison of RAPD Fingerprint Profile of Streptococcus iniae (Streptococcus iniae의 유전학적 다양성과 RAPD fingerprint profile의 비교)

  • Jeong Yong-Uk;Heo Moon-Soo
    • Journal of Life Science
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    • v.16 no.2 s.75
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    • pp.345-351
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    • 2006
  • Streptococcus sp. were isolated from cultured flounder (Paralichthys olivaceus) having Streptococcosis during 2004 to 2005 in Jeju Island. Ninety four Streptococcus iniae strains were isolated using biochemical test and multiplex PCR assay. Three genotypes (A, B, C-type) of S. iniae were appeared in the RAPD analysis and they showed international or local genetic polymorphism. Presently, S. iniae having A-type is a dominant S. iniae genotype in Jeju and showed band patterns at about 550, 850, 1000, 1300 and 2000 base pares. In this study, the reported P14 random primer, that used to distinguish serotypes of S. iniae could not be applied to distinguish Jeju island S. iniae's genetic polymorphism.

Antimicrobial activity of Artemisia princeps var. orientalis essential oil against fish pathogenic bacteria (어병(魚病) 세균(細菌)에 대한 쑥 정유(精油)의 항균력(抗菌力) 검색)

  • Kim, Young-Gill;Rho, Bum-Jin;Lee, Keun-Kwang
    • Journal of fish pathology
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    • v.7 no.2
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    • pp.113-117
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    • 1994
  • The antimicrobial activity of Artemisia princeps var. orientalis essential oil against a partial fish pathogenic bacteria was examined. The growth of Aeromonas hydrophila, Aeromonas salomonicida, Aeromonas sorbia, Edwardsiella tarda and Streptococcus sp. (yellowtail) were inhibited at concentrations of 1,000 to 2,000 ppm. The A. salmonicida was inhibited at 1,000 ppm, A. hydrophila, A. sorbia, E, tarda and Streptococcus sp. (yellowtail) at 1,500 ppm, but Vibrio anguillarum, Vibrio ordalii, Edwardsiella ictaluri and Streptococcus sp. (SF 1) were grown on 100-2,000 ppm.

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AN EXPERIMENTAL STUDY OF THE EFFECT OF FLUORIDE COMPOUNDS ON THE GROWTH OF STREPTOCOCCUS MUTANS (불소화합물이 Streptococcus mutans의 증식에 미치는 영향에 관한 실험적 연구)

  • In, In -Sook;Lee, Jong-Gap
    • Journal of the korean academy of Pediatric Dentistry
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    • v.11 no.1
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    • pp.151-157
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    • 1984
  • The purpose of this study was to compare the growth-inhibiting effect of sodium fluoride and stannous fluoride on a cariogenic strain of Streptococcus mutans exposed to the different concentration of $SnF_2$ and NaF The result were as follows: 1. The growth rate of the Streptococcus mutans was unaffected by 75 ppmF, slowed by 150 ppmF, and immediately arrested by 300 or 600 ppmF where increments of NaF were added to actively growing 12-hour broth culture. 2. $SnF_2$ slowed the growth rate at 75 ppmF, was to bactericidal initially at 150 and 300 ppmF, and was to totally bactercidal at 600 ppmF. 3. $SnF_2$ has been shown to be more effective than NaF in inhibiting the growth activity of Streptococcus mutans. 4. The inhibitory effect of fluoride compounds on the growth of Streptococcus mutans was increased in the decreased pH at concentration of 600 ppmF.

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Antibacterial effect of tea tree on Streptococcus mutans (Streptococcus mutans에 대한 티트리의 항균 효과)

  • Choi, Yu-Ri;Kang, Min-Kyung
    • Journal of Korean society of Dental Hygiene
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    • v.17 no.4
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    • pp.613-620
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    • 2017
  • Objectives: The purpose of this study is to investigate the antibacterial effect of Streptococcus mutans of tea tree ingredient. Methods: The experimental groups were each given with different concentrations (30 or 50 vol%) of tea tree prepared in saline solution. The control group applied only saline solution. The tea tree coating of the specimen were examined under a scanning electron microscope. For the antibacterial activity test of the tea tree, the contact angle of the tea tree- coated specimen's surface was analyzed. The antibacterial effect against Streptococcus mutans was determined by counting the colony forming units (CFU). The statical statics were evaluated by using one-way ANOVA and paired t-test. Results: The tea tree treated group of hydrophilic more than non treated group. Antibacterial experiments demonstrated that tee tree solution was effective against Streptococcus mutans. However there was no significant difference in depending solution concentration groups. Conclusions: The antimicrobial activity of the tea tree containing solution showed its potential for use as coating for denture and medical materials.

Studies on the mixed culture of Lactobacillus helveticus and Streptococcus thermophilus in milk - Growth characteristics and cultural conditions for the mixed culture - (Lactobacillus helveticus와 Streptococcus thermophilus의 혼합배양에 관한 연구 -생육특성 및 배양조건-)

  • 윤성식;박정길;유주현
    • Microbiology and Biotechnology Letters
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    • v.13 no.2
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    • pp.151-156
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    • 1985
  • To investigate the new Yoghurt starter, Lactobacillus helveticus YM-1, which was selected among 14 Lactobacillus strains, and Streptococcus thermophilus CH-1 were inoculated together in reconstituted non-fat skim milk medium and their growth characteristics and cultural conditions for the mixed culture were examined. The main results of this study were obtained as follows. The typical symbiotic growth was shown between the two strains and pH and temperature for optimal growth were 6.5 and 4$0^{\circ}C$, respectively. Heat treatment of milk was most effective at 10$0^{\circ}C$ for 30 min. The cell-free filtrate of Lactobacillus helveticus YM-1 had stimulatory effect on Streptococcus thermophilus CH-1 but the reverse case was slightly observed. Significant difference was observed in the proteolytic activities between Lactobacillus helveticus YM-1 and Streptococcus thermophilus CH-1. The former liberated 135$\mu\textrm{g}$ free amino acid per $m\ell$ of cultured milk, the latter 35$\mu\textrm{g}$ per $m\ell$.

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Complete genome sequence of Streptococcus intermedius ChDC B718 isolated from a human pulpitis lesion (사람 치수염 병소에서 분리된 Streptococcus intermedius ChDC B718의 유전체 염기서열 해독)

  • Park, Soon-Nang;Roh, Hanseong;Lim, Yun Kyong;Kook, Joong-Ki
    • Korean Journal of Microbiology
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    • v.53 no.2
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    • pp.131-133
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    • 2017
  • Streptococcus intermedius is a Gram-positive, obligately anaerobic, nonsporeforming, and nonmotile cocci. S. intermedius is a member of oral flora and is endodontic infection, respiratory infections, infective endocarditis, brain abscess, and liver abscess. Streptococcus intermedius ChDC B718 (= KCOM 1545) was isolated from a human pulpitis lesion. Here, we present the complete genome sequence of S. intermedius ChDC B718.

Effect of Single or Mixed Culture of Lactobacillus bulgaricus and Streptococcus thermophilus on Fermentation Characteristics of Buckwheat Sprout-added Yoghurt (Lactobacillus bulgaricus 와 Streptococcus thermophilus 의 단독 또는 혼합배양한 메밀싹 첨가 요구르트의 발효 특성)

  • Kang, Ha-Ni;Kim, Chul-Jai
    • Journal of the Korean Society of Food Culture
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    • v.25 no.1
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    • pp.76-81
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    • 2010
  • This study was conducted to evaluate the influence of Lactobacillus bulgaricus and/or Sterptococcus thermophilus on the fermentation of yoghurt containing 5% (w/v) buckwheat sprouts. The results revealed that after 12 hours of fermentation the appropriate pH, titratable acidity and number of viable cells were attained. At that time, the rutin content of the buckwheat sprout-added yoghurt prepared by the mixed culture method was not changed, but the quercetin content increased greatly. Conversely, the rutin content of yoghurt that only contained Streptococcus thermophilus was decreased while the quercetin content was increased. The total phenol contents as well as the DPPH radical scavenging activities of both the mixed culture and Streptococcus thermophilus yoghurt did not differ significantly. Taken together, the results revealed that the use of a mixed culture of Lactobacillus bulgaricus and Streptococcus thermophilus during the preparation of buchwheat sprout-added yoghurt was desirable due to the decrease in pH and increase in titratable acidity and viable cells that occurred after 12 hr of fermentation. Moreover, phytochemicals in buckwheat sprouts such as rutin, quercetin and phenol compounds were comparatively increased during fermentation and influenced the antioxidant activity in buckwheat sprout-added yoghurt.

Effect of Green Tea Powder on the Growth Inhibition of Oral Bacteria in Yoghurt (가루녹차 첨가 요구르트에 의한 충치 원인균 증식 억제 효과)

  • Jung Da-Wa;Park Shin-In
    • Food Science of Animal Resources
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    • v.25 no.4
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    • pp.500-506
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    • 2005
  • This study was carried out to obtain knowledges on the survival of Streptococcus mutans in the yoghurt added with green tea powder. The growth inhibition of green tea powder on the food borne pathogens and oral bacteria was measured by total microbial count, Among the tested food borne pathogens, the growth of Staphylococcus aureus and Salmonella enteritidis were not significantly affected by the addition of green tea powder, but green tea powder showed the growth inhibition effect on Escherichia coli O157:H7. The number of surviving Streptococcus mutans cell was decreased by $0.56\~0.99log$ cycle after 24 hem incubation by the addition of $0.5\~2.5\%$ green tea powder in the medium. And also the viable cell count of surviving Streptococcus mutans cells (initial inoculum $3.4\times10^7CFU/mL$) were decreased to $1.4\times10^4\~7.2\times10^4 CFU/mL$ after 48 hours incubation when $0.5\~2.5\%$ green tea powder were added to the drinkable yoghurt, Growth of Streptococcus mutans was strongly inhibited by the addition and incubation of green tea powder for 48 hum in the yoghurt.