• Korean Journal of Agricultural Science
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• v.47 no.3
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• pp.673-681
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• 2020

Human enteric Adenovirus 41 (HueAdV-41) is a major waterborne virus that causes human gastroenteritis and is classified as a viral group I double-strand DNA virus, Adenoviridae. HueAdV-41 has been detected with the polymerase chain reaction (PCR) in various samples such as ground water. However, the PCR-based diagnostic method has problems such as reaction time, sensitivity, and specificity. Thus, the loop-mediated isothermal amplification (LAMP) assay has emerged as an excellent method for field applications. In this study, we developed a LAMP system that can rapidly detect HueAdV-41 with high specificity and sensitivity. HueAdV-41 specific LAMP primer sets were tested through a specific, non-specific selection and sensitivity test for three prepared LAMP primer sets, of which only one primer set and optimum reaction temperature were selected. The developed LAMP primer set condition was confirmed as 63℃, and the sensitivity was 1 copy. In addition, to confirm the system, a LAMP positive reaction was developed with the restriction enzyme Taq I (T/GCC). The developed method in this study was more specific, rapid (typically within 2 - 3 hours), and highly sensitive than that of the conventional PCR method. To evaluate and verify the developed LAMP assay, an artificial infection test was done with five cDNAs from groundwater samples, and the results were compared to those of the conventional PCR method. We expect the developed LAMP primer set will be used to diagnose HueAdV-41 from various samples.

• Journal of the Korean Geotechnical Society
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• v.33 no.1
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• pp.39-56
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• 2017

Distribution of both axial force and skin friction should be investigated in order to estimate pull-out capacity of ground anchors. Numerical method of computing load-transfer characteristics of the ground anchors, however, has not been specified and studies on this area are not sufficient. This study suggested the numerical method of simulating the characteristics of axial force and skin friction distribution against the tension type ground anchors. Also, debonding behaviors of skin friction and axial force were calculated by the suggested numerical method as a function of load levels. As a result of the review, it is confirmed that the distributions of axial force and skin friction by the suggested numerical method are relatively similar to those of field test results.

• The Journal of the Korean bone and joint tumor society
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• v.3 no.2
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• pp.69-79
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• 1997

Objective : The objective of this study was to compare expression of various proto-oncogenes and rates of apoptosis in osteosarcoma patients. Modulation of apoptosis may influence resistance to chemotherapy and therefore affect the outcome of cancer treatment. Osteosarcoma is one of the most fatal malignancies in young adolescents and investigation of the role of apoptotic cell death is warranted in relation to chemotherapy and tumor outcome. Design : The terminal deoxynucleotidyl transferase to exposed 3'-hydroxyl termini of DNA (TUNEL method) staining method has been applied for the in situ detection of DNA double strand breaks. Patients : Thirty-three osteosarcomas in various stages of differentiation from twenty-nine patients were investigated immunohistochemically for p53, Bcl-2 and TUNEL method for apoptosis. Results and conclusion; We have found that higher level of wild type p53 were correlated with enhanced expression of apoptosis. Increased apoptosis rates were found in cases of negative Bcl-2 expression. In the present study, we have concluded that a significant proportion of osteosarcoma, a tumor in which resistance to chemotherapy often occurs, express high levels of p53 and low levels of Bcl-2. Our data provide further evidence for cross-talk between Bcl-2 and p53 and suggests that these genes are important determinants of drug-induced apoptosis.

• Journal of Life Science
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• v.22 no.3
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• pp.293-297
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• 2012

The movement of vesicles from the neuronal cell body to specific destinations requires molecular motors. The squid giant axon represents a powerful model for studies of the axonal transport mechanism because the axoplasm can readily be separated from the sheath by simple extrusion. In a previous study, vesicular movements in the axoplasm of the squid giant axon were inhibited by the kinesin antibody. In the present study, we cloned and sequenced the cDNAs for squid brain KIFs. Amplification of the conserved nucleotide sequences of the motor domain by polymerase chain reaction (PCR) using first-strand cDNAs of the squid optic lobe identified six new KIF proteins. Motif analysis of the motor domains revealed that the squid KIFs are homologous to the consensus sequences of the mouse KIFs. The phylogenetic tree generated by using the maximum parsimony (MP) method, the neighbor-joining (NJ) method, the minimum evolution (ME) method, and the maximum likelihood (ML) method showed that squid KIFs are closest to mouse KIFs. These data prove the phylogenetic relationships between squid KIFs and mouse ones.

• Journal of Microbiology and Biotechnology
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• v.33 no.9
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• pp.1228-1237
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• 2023

The CRISPR-Cas system has emerged as the most efficient genome editing technique for a wide range of cells. Delivery of the Cas9-sgRNA ribonucleoprotein complex (Cas9 RNP) has gained popularity. The objective of this study was to develop a quantitative polymerase chain reaction (qPCR)-based assay to quantify the double-strand break reaction mediated by Cas9 RNP. To accomplish this, the dextransucrase gene (dsr) from Leuconostoc citreum was selected as the target DNA. The Cas9 protein was produced using recombinant Escherichia coli BL21, and two sgRNAs were synthesized through in vitro transcription to facilitate binding with the dsr gene. Under optimized in vitro conditions, the 2.6 kb dsr DNA was specifically cleaved into 1.1 and 1.5 kb fragments by both Cas9-sgRNA365 and Cas9-sgRNA433. By monitoring changes in dsr concentration using qPCR, the endonuclease activities of the two Cas9 RNPs were measured, and their efficiencies were compared. Specifically, the specific activities of dsr365RNP and dsr433RNP were 28.74 and 34.48 (unit/㎍ RNP), respectively. The versatility of this method was also verified using different target genes, uracil phosphoribosyl transferase (upp) gene, of Bifidobacterium bifidum and specific sgRNAs. The assay method was also utilized to determine the impact of high electrical field on Cas9 RNP activity during an efficient electroporation process. Overall, the results demonstrated that the qPCR-based method is an effective tool for measuring the endonuclease activity of Cas9 RNP.

• Journal of Radiation Protection and Research
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• v.24 no.2
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• pp.93-99
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• 1999

The alkaline single-cell gel electrophoresis (SCGE) assay, called the comet assay, has been applied to the detection of DNA damage from a number of chemical and biological factors in vivo and in vitro. The comet assay is a novel method to assess DNA single-strand breaks, alkali-labile sites in individual cells. The effect of peach kernel extracts on radiation-induced DNA damage in human blood lymphocytes was evaluated by the SCGE assay. The lymphocytes, with or without pretreatment of the extracts, were exposed to 0, 0.1, 0.3, 0.5, 1.0 and 2.0 Gy of $^{60}Co$ gamma ray. Significantly increased tail moment, which was a marker of DNA strand breaks in the comet assay, showed an excellent dose-response relationship. The treatment of the peach kernel extracts reduced the DNA damage by 30 % in irradiated groups as compared to that in non-treated control groups. The result indicates that the extracts shows radioprotective effect on lymphocyte DNA when assessed by the comet assay.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.5
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• pp.569-576
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• 2019

PSC-Edge Rahmen Bridge makes low thickness and long span by introducing prestressed force to the edge girder and reducing positive moment. In the bridge, diagonal tension cracks occurred in the direction of $45^{\circ}$ to outer side of the girder after the temporary bent supported on the lower part of the upper slab and the secondary strand is tensioned on the girder. Researches on stress distribution and burst crack behavior of pre-stress anchorage has been conducted, it is difficult to analyze an obvious cause due to difference between actual shape and boundary condition. This study performed 3D frame analysis with additional boundary condition of temporary bent, the maximum compression stress occurred in the girder and there was a limit to identify the cause. It performed 3D Solid analysis with LUSAS 16.1 and the maximum principal tensile stress occurred at the boundary between the girder and the slab. As analyzing required reinforcement quantity at obtuse angle of the girder with the maximum principal tensile stress and directional cosine, reinforcement quantity was insufficient. Additional bridges have increased reinforcement quantity and extended area and crack was not occurred. It is expected that cracks on the girder during construction could be controlled by applying the proposed method to PSC-Edge Rahmen Bridge.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.16 no.12 s.117
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• pp.1279-1285
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• 2006

In this study, structural deformation estimation using displacement-strain relationship is investigated. When displacements of a structure cannot be measured directly, estimation of displacements using strain data can be an alternative solution. Additionally, the deformation of the whole structure as well as the displacement at the point of interest can be estimated. Strain signals are obtained front Fiber Bragg Grating(FBG) sensors that have an excellent multiplexing ability. Some experiments were performed on two beams and a plate to which FBG sensors were attached in the laboratory. Strain signals from FBG sensors along a single strand of optical fiber were obtained through wavelength division multiplexing(WDM) method. The beams and the plate structures were subjected to various loading conditions, and deformed shapes were reconstructed from the displacement-strain transformation relationship. The results show good agreements with those measured directly from laser sensors. Moreover, the whole structural shapes of the beams and the plate were estimated using only some strain sensors.

• Korean Institute of Interior Design Journal
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• v.14 no.1
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• pp.37-44
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• 2005

Currently, the space design has been expressed the space in kinetic design by digital technology. To look into the concept of digital design, there is the tendency to pursue the harmony of the nature. The digital space designer, Greg Lynn who has been paid attention by international researchers. To compared with the reputation of his works, the information about him has been limited to us. The purpose of this study was to investigate the Greg Lynn's digital design matrix toward the design process in his representative 11 works in his website; www.glform.com. The contents analyses methods were used in this study. Greg Lynn's internet website survey was carried out in the respects of thinking method, space formative language and animate form. The major results of this study are as follows: \circled1 Lynn's design concept and digital methodology were affected by Paolo Soleri and Peter Eisenman: natural architectural concept and digital animate form \circled2 Lynn's space formative languages were 10 items; blob, blob, fold, strand, shred, flower, skin, teeth, branch and lattice \circled3 Lynn's digital design matrix was divided into 3 types; MS(Mass + Structure), PC(Path + Circulation) and FD(Form + Detail) \circled4 According to the analysis of longitudinal, his works have been changed from the MS and PC to FD. This research will be a basic reference to understand digital space design.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.20 no.11
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• pp.3433-3440
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• 1996

This paper was investigation of the stres corrosion cracking(SCC) mechanism and the properties of corrosion fracture surface of glass fiber reinforced plastics(GFRP) produced by hand lay up(HLU) method in synthetic sea water. Test material is GFRP, that was used vinylester type epoxy acrylate resin and an unsaturated polyester as the matrix and the chopped strand mat(CSM) type E-glss fiber as the reinforcement. The slow strain rate test(SSRT) was performed on dry, wet and saturated wet specimens in sea water. Here the pH concentration of synthetic sea water was 8.2 and the strain rate is 1 x $10^{-6}$($sec^{-1}$) and test temperature ranges varied from $-60^{\circ}C$ to $80^{\circ}C$. It could be confirmed the fact that wet specimens tested at a particular test temperature ranges were appeared the eviences of SCC such as con-planar, mirror and hackle zone. Moreover, SCC of GFRP in sea water was characterised by falt fracture surfaces with only small amounts of fiber pull-out, in partial.