• 약학회지
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• 제31권2호
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• pp.116-125
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• 1987

It is still difficult and time consuming to obtain cDNA sequences that contain the entire nucleotide sequence of the corresponding mRNA. A rapid and high efficient cloning method to obtain full-length cDNA segments is thus developed. The cloning procedure described here consists of the construction of oligo(dT)-tailed vector primer using pWR34 plasmid, polyadenylation of mRNA-cDNA heteroduplex using terminal deoxytransferase, and replacement of MRNA strand with DNA by RNase H and DNA polymerase I. The restriction endonuclease analysis shows that the size of inserted-cDNA is in the range of 1.5~4.0 kb long suggesting that most of cloned cDNA are full-length or nearly full-length cDNA. The plasmid-DNA recombinants obtained were 4$\times$$10^5$~$10^{6}$ per $\mu\textrm{g}$ of rat liver poly (A$^+$)mRNA, which is 4 to 10 fold higher cloning efficiency in comparison to the presently used methods for full-length cDNA cloning. The results indicate that the described cloning system is much simpler, less time consuming, and very efficient cloning method to construct a cDNA library.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2006년도 제27회 추계학술대회논문집
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• pp.247-250
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• 2006

기존 연구를 통해 고체추진제 연소속도 측정을 위한 초음파 측정 시스템 및 연소속도 산정 기법을 개발하였으며, 개발 시스템을 적용하여 composite 추진제의 연소속도를 측정하였다. 본 연구에서는 연소 압력 증가에 따라 연소속도가 비선형적으로 변하는 double base형 고체 추진제 인 N-5와 AA-2 고체추진제의 연소속도를 개발 시스템을 이용하여 측정하였으며, 스트랜드버너법으로 측정된 값과의 비교를 통해 측정결과의 신뢰성을 평가하였다.

• Computers and Concrete
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• 제22권1호
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• pp.71-81
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• 2018

Introduction of prestressed concrete slabs based on post-tensioned (PT) method aids in constructing larger spans, more useful floor height, and reduces the total weight of the building. In the present paper, for the first time, simulation of 32 two-way PT slab-edge column connections is performed and verified by some existing experimental results which show good consistency. Finite element method is used to assess the performance of bonded and unbonded slab-column connections and the impact of different parameters on these connections. Parameters such as strand bonding conditions, presence or absence of a shear cap in the area of slab-column connection and the changes of concrete compressive strength are implied in the modeling. The results indicate that the addition of a shear cap increases the flexural capacity, further increases the shear strength and converts the failure mode of connections from shear rigidity to flexural ductility. Besides, the reduction of concrete compressive strength decreases the flexural capacity, further reduces the shear strength of connections and converts the failure mode of connections from flexural ductility to shear rigidity. Comparing the effect of high concrete compressive strengths versus the addition of a shear cap, shows that the latter increases the shear capacity more significantly.

• 대한기계학회논문집
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• 제13권3호
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• pp.472-478
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• 1989

본 연구에서는 FRP의 파괴인성평가를 위한 기초적인 자료를 얻기 위하여 FRP 중에서도 널리 사용되고 있는 SMC재를 준비하고 3점 굽힘시험을 실시하여 노치선단에서의 손상거동 및 균열진전저항유선으로부터 안정파괴개시점을 검토하였다.

• 콘크리트학회논문집
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• 제11권3호
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• pp.141-152
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• 1999

Precast prestressed double-tee slab may be designed by the PCI Design Handbook. It is based on the bridge construction and is required for reorganization for the use of buildings in the domestic construction environments. Much enhanced sections are developed from the reforming process on the determined design factors in the previous experimental works on double tees. Pre-determined shape, reinforcement detail, and 5000 psi concrete strength can not be expected as the best solution for the domestic construction requirements because large amount of use on that systems are anticipated. Flexural tests are performed on four full-scale 12.5m proto-type models, "least depth double tee", which are resulted from the optimization process. Domestic superimposed live load regulation, domestic material properties which is available to product, building design requirements and economy in construction are considered as the main factors to establish. the first two sections are double tee section for 1.2 ton/$\m^2$ market live load with straight and one-point depressed strands and the second two are for 0.6 ton/$\m^2$ parking live load with those strand types. All of the specimens tested fully comply with the flexural strength requirements as specified by ACI 318-95. However, the research has shown that following improved considerations are needed for better result in practice. The locations and method of connection for the lowest bottom mild bar, connection method between precast and cast-in-place concrete, and dap-end reinforcement are need to be improved.

• Smart Structures and Systems
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• 제18권3호
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• pp.585-599
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• 2016

Steel cables serve as the key structural components in long-span bridges, and the force state of the steel cable is deemed to be one of the most important determinant factors representing the safety condition of bridge structures. The disadvantages of traditional cable force measurement methods have been envisaged and development of an effective alternative is still desired. In the last decade, the vision-based sensing technology has been rapidly developed and broadly applied in the field of structural health monitoring (SHM). With the aid of vision-based multi-point structural displacement measurement method, monitoring of the tensile force of the steel cable can be realized. In this paper, a novel cable force monitoring system integrated with a multi-point pattern matching algorithm is developed. The feasibility and accuracy of the developed vision-based force monitoring system has been validated by conducting the uniaxial tensile tests of steel bars, steel wire ropes, and parallel strand cables on a universal testing machine (UTM) as well as a series of moving loading experiments on a scale arch bridge model. The comparative study of the experimental outcomes indicates that the results obtained by the vision-based system are consistent with those measured by the traditional method for cable force measurement.

• Asian-Australasian Journal of Animal Sciences
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• 제32권7호
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• pp.949-955
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• 2019

Objective: The present study was to investigate the association of polymorphisms in exon-9 of the bone morphogenetic protein receptor-1B (BMPR-1B) gene (C864T) with litter size in 240 Dorset, 232 Mongolian, and 124 Small Tail Han ewes. Methods: Blood samples were collected from 596 ewes and genomic DNA was extracted using the phenol: chloroform extraction method. The 304-bp amplified polymerase chain reaction product was analyzed for polymorphism by single-strand conformation polymorphism method. The genotypic frequency and allele frequency of BMPR-1B gene exon-9 were computed after sequence alignment. The ${\chi}^2$ independence test was used to analyze the association of genotypic frequency and litter size traits with in each ewe breed, where the phenotype was directly treated as category. Results: The results indicated two different banding patterns AA and AB for this fragment, with the most frequent genotype and allele of AA and A. Calculated Chi-square test for BMPR-1B gene exon-9 was found to be more than that of p value at the 5% level of significance, indicating that the population under study was in Hardy-Weinberg equilibrium for all ewes. The ${\chi}^2$ independence test analyses indicated litter size differences between genotypes was not the same for each breed. The 304-bp nucleotide sequence was subjected to BLAST analysis, and the C864T mutation significantly affected litter size in singletons, twins and multiples. The heterozygosity in exon-9 of BMPR-1B gene could increase litter size for all the studied ewes. Conclusion: Consequently, it appears that the polymorphism BMPR-1B gene exon-9 detected in this study may have potential use in marker assisted selection for litter size in Dorset, Mongolian, and Small Tail Han ewes.

• 한국암반공학회:학술대회논문집
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• 한국암반공학회 2006년도 춘계학술발표회 논문집
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• pp.195-203
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• 2006

철도지하횡단공사는 횡단구조물 축조시 개착식과 비개착식 공법으로 구별되는데 개착식 공법은 열차의 비운행시간을 고려하여 시공하는 극히 제한적인 공법인데 반하여 비개착식은 열차의 속도 및 운행시간에 제약을 받지 않는 특징을 갖고 있으며 안전하고 신속한 시공의 장점을 갖고 있다. 비개착식 공법 중 Front Jacking 공법은 소구경의 강관을 압입하여 구조물과의 마찰력과 토압을 최소화시킨 후 현장에서 제작한 전단면 Precast Box 선단부에 PC강연선과 유압Jack을 이용하여 박스를 지중에 견인하는 공법으로 시공시 안정성과 열차의 정시성 확보가 유리하여 많이 적용되고 있다. 이 공법은 박스단면의 크기 및 연장, 지반조건에 관계없이 시공가능하며 친환경적인 시공을 할 수 있다. 본 고에서는 Front Jacking 공법이 적용된 대전동서관통대로의 철도횡단 시공사례를 소개하고자 한다.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2005년도 춘계학술대회 논문집
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• pp.646-654
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• 2005

철도지하횡단공사는 횡단구조물 축조시 개착식과 비개착식 공법으로 구별되는데 개착식 공법은 열차의 비운행시간을 고려하여 시공하는 극히 제한적인 공법인데 반하여 비개착식은 열차의 속도 및 운행시간에 제약을 받지 않는 특징을 갖고 있으며 안전하고 신속한 시공의 장점을 갖고 있다. 비개착식 공법 중 Front Jacking 공법은 소구경의 강관을 압입하여 구조물과의 마찰력과 토압을 최소화 시킨 후 현장에서 제작한 전단면 Precast Box 선단부에 PC강연선과 유압Jack을 이용하여 박스를 지중에 견인하는 공법으로 시공시 안정성과 열차의 정시성 확보가 유리하여 많이 적용되고 있다. 이 공법은 박스단면의 크기 및 연장, 토질조건에 관계없이 시공가능하며 친환경적인 시공을 할 수 있다. 본 고에서는 Front Jacking 공법이 적용된 대전동서관통대로의 철도횡단 시공사례를 소개하고자 한다.

• Structural Engineering and Mechanics
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• 제54권1호
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• pp.105-119
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• 2015

Structural damage detection using modal strain energy (MSE) is one of the most efficient and reliable structural health monitoring techniques. However, some of the existing MSE methods have been validated for special types of structures such as beams or steel truss bridges which demands improving the available methods. The purpose of this study is to improve an efficient modal strain energy method to detect and quantify the damage in complex structures at early stage of formation. In this paper, a modal strain energy method was mathematically developed and then numerically applied to a fixed-end beam and a three-story frame including single and multiple damage scenarios in absence and presence of up to five per cent noise. For each damage scenario, all mode shapes and natural frequencies of intact structures and the first five mode shapes of assumed damaged structures were obtained using STRAND7. The derived mode shapes of each intact and damaged structure at any damage scenario were then separately used in the improved formulation using MATLAB to detect the location and quantify the severity of damage as compared to those obtained from previous method. It was found that the improved method is more accurate, efficient and convergent than its predecessors. The outcomes of this study can be safely and inexpensively used for structural health monitoring to minimize the loss of lives and property by identifying the unforeseen structural damages.