• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.31-32
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• 2005

Neoagaro-oligosaccharides are produced only by enzymatic degradation of agarose by ${\beta}-agarase.^{1)}$ Neoagaro-oligosaccharides inhibit the growth of bacteria, slow the rate of degradation of starch, are used as low-calorie additives to improve food quality, and have macrophage-stimulating activity. Furthermore, neoagarobiose is a rare reagent that has both moisturizing effect on skin and whitening effect on melanoma $cells.^{2)}$ An agar-degrading marine bacterium was isolated from the sea water at the northeast coast in Cheju island, Korea. The strain was gram negative, aerobic, and motile rod. The 16S rRNA of the strain had the closest match of 98% homology, with that from Agarivorans albus. On the basis of several phenotypic characters and a phylogenetic analysis, this strain was designated Agarivorans sp. JA-1. In solid agar plate, Agarivorans sp. JA-1 produced a diffusible agarase that caused agar softening around the colonies. Agarivorans sp. JA-1 was cultured for 36 hr in marine broth 2216 (Difco, USA) and the supernatant that containing an extracellular ${\beta}-agarase$ was prepared by centrifugation of culture media. The enzyme exhibited relatively strong activity at $40^{\circ}C$ and was stable up to $60^{\circ}C$. Using PCR primers derived from the ${\beta}-agarase$ gene of Vibrio sp., the gene encoding ${\beta}-agarase$ from Agarivorans sp. JA-1 was cloned and sequenced. The structural gene consists of 2931 bp encoding 976 amino acids with a predicted molecular weight of 107,360 Da. The deduced amino acid sequence showed 99% and 34% homology to $agaA^{2)}$ and $agaB^{2)}$ genes for ${\beta}-agarase$ from Vibrio sp., respectively. The expression plasmid for ${\beta}-agarase$ gene of Agarivorans sp. JA-1 is being constructed and the recombinant enzyme will be biochemically characterized.

• 열처리공학회지
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• 제4권4호
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• pp.24-33
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• 1991

The effects of thermomechanical treatments on microstructure and fatigue properties of 7050 Al alloy were investigated. The precipitation kinetics changed to a faster rate due to cold deformation employed in this special TAHA thermomechanical treatments including pre-aging, plastic deformation and two step final-aging. The G.P. zones in the under-aged condition were cut by dislocations and dissolved during the plastic deformation. During the low cycle fatigue, the T6' condition showed cyclic hardening behavior whereas the TMT5, TMT27 and T76 conditions showed cyclic softening at above 0.7% total strain amplitudes. The ${\Delta}K_{th}$ value of TMT27 was improved more than two times, compared with that of T76 condition. The T6' with small shearable precipitates resulted in the markedly high ${\Delta}K_{th}$ value. This is thought to be resulted from dislocation reversibility and roughness-induced crack closure due to planarity of slip.

• Geomechanics and Engineering
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• 제34권4호
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• pp.409-424
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• 2023

Response of the pipeline crossing fault is considered as the large strain problem. Proper estimation of the pipeline response plays important role in mitigation studies. In this study, an advanced continuum modeling including material non-linearity in large strain deformations, hardening/softening soil behavior and soil-pipeline interaction is applied. Through the application of a fully nonlinear analysis based on an explicit finite difference method, the mechanics of the pipeline behavior and its interaction with soil under large strains is presented in more detail. To make the results useful in oil and gas engineering works, a continuous pipeline of two steel grades buried in two clayey soil types with four different crossing angles of 30°, 45°, 70° and 90° with respect to the pipeline axis have been considered. The results are presented as the fault movement corresponding to different damage limit states. It was seen that the maximum affected pipeline length is about 20 meters for the studied conditions. Also, the affected length around the fault cutting plane is asymmetric with about 35% and 65% at the fault moving and stationary block, respectively. Local buckling is the dominant damage state for greater crossing angle of 90° with the fault displacement varying from 0.4 m to 0.55 m. While the tensile strain limit is the main damage state at the crossing angles of 70° and 45°, the cross-sectional flattening limit becomes the main damage state at the smaller 30° crossing angles. Compared to the stiff clayey soil, the fault movement resulting 3% tensile strain limit reach up to 40% in soft clayey soil. Also, it was seen that the effect of the pipeline internal pressure reaches up to about 40% compared to non-pressurized condition for some cases.

• 대한기계학회논문집A
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• 제26권5호
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• pp.896-903
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• 2002

The susceptibility of SCC for the weldment and PWHT specimens of HT-60 steel was evaluated using a slow strain rate method under applied potential by means of the potentiostat in synthetic seawater. In case of the parent, anodic polarization voltage was inappropriate in elongating the time to failure(TTF). -0.8V corresponding to cathodic protection range is most effective in improving the SCC resistance against corrosive environment. In case of the weldment, the values of reduction of area(ROA) and TTF at -0.68V corresponding to cathodic polarization value were 45.2% and 715,809sec which were the largest and longest life among other applied potentials. Those were vise versa at -1.1V. In case of the PWHT specimens, TTF and ROA at -0.68V was longest and largest like the weldment. Besides, PWHT is effective in prolonging the time to failure of the welded off-shore structure due to softening of effect. Regardless of the weldment and PWHT specimen, as corrosion rate gets higher, TTF becomes shorter and deformation behaviour for the weldment and PWHT specimen at -1.1V was shown to be irregular. Finally, it was found that specimens showed brittle fracture at -1.1V, but more ductile fracture accompanying the micro-cracks at applied potential of -0.68V.

• Structural Engineering and Mechanics
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• 제68권6호
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• pp.747-760
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• 2018

In the present study, a methodology for developing fragilities of arch concrete dams to assess their performance against seismic hazards is introduced. Firstly, the probability risk and fragility curves are presented, followed by implementation and representation of the way this method is used. Amirkabir arch concrete dam was subjected to non-linear dynamic analyses. A modified three dimensional rotating smeared crack model was used to take the nonlinear behavior of mass concrete into account. The proposed model considers major characteristics of mass concrete. These characteristics are pre-softening behavior, softening initiation criteria, fracture energy conservation, suitable damping mechanism and strain rate effect. In the present analysis, complete fluid-structure interaction is included to account for appropriate fluid compressibility and absorptive reservoir boundary conditions. In this study, the Amirkabir arch concrete dam is subjected to a set of 8 three-component earthquakes each scaled to 10 increasing intensity levels. Using proposed nonlinear smeared crack model, nonlinear analysis is performed where the structure is subjected to a large set of scaled and un-scaled ground motions and the maximum responses are extracted for each one and plotted. Based on the results, fragility curves were plotted according to various and possible damages indexes. Discrete damage probabilities were calculated using statistical methods for each considered performance level and incremental nonlinear analysis. Then, fragility curves were constructed based on the lognormal distribution assumption. Two damage indexes were introduced and compared to one another. The results indicate that the dam has a proper stability under earthquake conditions at MCE level. Moreover, displacement damages index is more conservative and impractical in the fragility analysis than tensional damage index.

• 한국지반공학회지:지반
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• 제12권6호
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• pp.115-126
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• 1996

삼축압축시험의 결과를 이용해 2차광물성분을 많이 포함하고 있는 모앙이 편마암인 풍화잔적토의 압축 및 강도특성을 분석하여, 모암 및 광물성분비가 다른 기존의 연구결과와 비교하여 풍화잔적토의 모암과 광물성부네 따른 강도정수의 차이 및 거동특성을 파악하기 위한 근간을 제시하고자 본 연구를 수행하였으며, 치를 위하여 CIV, CID, CKoU, CKoD 등의 삼축압축시험을 실시하였다. 기원 및 광물성분이 다른 풍화잔적토라도 정규압축곡선(NCL)의 기울기는 유사한 양상을 보이며, 압축시 입자파쇄효과로 인하여 약 85%의 소성변형량을 나타냈다. 전단시 또이동 시료는 김천 및 안동 시료와는 다르게 응력연화현상을 나타내 기원 및 구성광물에 따라 다른 거동을 보여줌을 알 수 있었다. 펀단저항각은 시료가 석영과 장석성분 등의 1차 광물과 2차광물의 구성성 분에 따라 달라짐을 알 수 있었으며, 반면에 간극수압계수는 기원 및 광물성분을 막론하고 1 이상의 큰 값을 나타냈다.

• 대한토목학회논문집
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• 제4권4호
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• pp.1-11
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• 1984

콘크리트의 복잡한 파괴거동(破壞擧動)을 모델할 수 있는 비선형파괴이론(非線型破壞理論)이 본(本) 논문(論文)에서 유도되었다. 본(本) 이론(理論)은 콘크리트균열끝의 파괴진행대(破壞進行帶)에서의 미세균열현상(微細龜裂現象)과 변형연화현상(變形軟化現象)으로 인한 비선형거동(非線型擧動)에 그 근거(根據)를 두고 있다. 이로부터 실제적이고도 단순화(單純化)된 파괴해석(破壞解析)모델이 유도되었으며, 이 단순화된 모델은 파괴진행대(破壞進行帶)의 변화에도 콘크리트의 파괴(破壞)에너지를 일정하게 유지할 수 있는 특정을 가지고 있다. 파괴해석(破壞解析)에 비선형응력(非線型應力)-변형거동(變形擧動)을 이용함으로써 균열면에 평행하게 작용하는 압축응력에 대한 영향이 쉽게 고려될 수 있다. 본 모델을 콘크리트의 많은 파괴실험자료와 비교한 결과 만족스러운 결과를 얻었으며, 종래의 선형파괴이론에 의한 결과는 실제 콘크리트 실험결과와 많은 차이를 보이고 있음을 알 수 있다. 콘크리트 구조의 균열 및 파괴해석에 필요한 콘크리트 파괴에너지의 근사공식이 본 이론으로부터 유도되었다. 끝으로, 철근콘크리트에 대한 파괴이론의 응용 및 확장연구 문제가 토의되었다.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2004년도 추계학술대회논문집
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• pp.211-214
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• 2004

적절한 가공조건을 통하여, 중 탄소강에서 SIDT를 적용하여 페라이트-시멘타이트 미세조직이 압연 직후에 얻어졌다. 또한 이와 같은 조건은 압하온도 $690^{\circ}C$, 높이 감소율 $70\%$ (변형량 1.2) 및 변형율 속도 1/s에서 가공하였을 때 얻어졌으며, 가공 직후 $730^{\circ}C$로 승온하여 약 30분간 유지 후 서냉한 조건에서 매우 연한 미세조직이 얻어짐을 알 수 있었다. 이는 기존 20 시간 이상의 구상화 열처리에 비해 매우 짧은 시간이며, 구상화 열처리를 통한 경험한 미세조직과 유사한 특성을 나타낼 것으로 예상된다.

• Geomechanics and Engineering
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• 제18권4호
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• pp.353-362
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• 2019

Soil-rock mixture (S-RM) is an inhomogeneous geomaterial that is widely encountered in nature. The mechanical and physical properties of S-RM are important factors contributing towards different deformation characteristics and unstable modes of the talus slope. In this paper, the equivalent substitution method was employed for the preparation of S-RM test samples, and large-scale triaxial laboratory tests were conducted to investigate their mechanical parameters by varying the water content and confining pressure. Additionally, a simplified geological model based on the finite element method was established to compare the stability of talus slopes with different strength parameters and in different excavation and support processes. The results showed that the S-RM samples exhibit slight strain softening and strain hardening under low and high water content, respectively. The water content of S-RM also had an effect on decreasing strength parameters, with the decrease in magnitude of the cohesive force and internal friction angle being mainly influenced by the low and high water content, respectively. The stability of talus slope decreased with a decrease in the cohesion force and internal friction angle, thereby creating a new shallow slip surface. Since the excavation of toe of the slope for road construction can easily cause a landslide, anti-slide piles can be used to effectively improve the slope stability, especially for shallow excavations. But the efficacy of anti-slide piles gradually decreases with increasing water content. This paper can act as a reference for the selection of strength parameters of S-RM and provide an analysis of the instability of the talus slope.

• Structural Engineering and Mechanics
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• 제75권6호
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• pp.643-657
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• 2020

This study presents experimental and numerical investigations on circular steel tube confined ultra high performance concrete (UHPC) columns under axial compression. The plain UHPC without fibers was designed to achieve a compressive strength ranged between 150 MPa and 200 MPa. Test results revealed that loading on only the UHPC core can generate a significant confinement effect for the UHPC core, thus leading to an increase in both strength and ductility of columns, and restricting the inherent brittleness of unconfined UHPC. All tested columns failed by shear plane failure of the UHPC core, this causes a softening stage in the axial load versus axial strain curves. In addition, an increase in the steel tube thickness or the confinement index was found to increase the strength and ductility enhancement and to reduce the magnitude of the loss of load capacity. Besides, steel tube with higher yield strength can improve the post-peak behavior. Based on the test results, the load contribution of the steel tube and the concrete core to the total load was examined. It was found that no significant confinement effect can be developed before the peak load, while the ductility of post-peak stage is mainly affected by the degree of the confinement effect. A finite element model (FEM) was also constructed in ABAQUS software to validate the test results. The effect of bond strength between the steel tube and the UHPC core was also investigated through the change of friction coefficient in FEM. Furthermore, the mechanism of circular steel tube confined UHPC columns was examined using the established FEM. Based on the results of FEM, the confining pressures along the height of each modeled column were shown. Furthermore, the interaction between the steel tube and the UHPC core was displayed through the slip length and shear stresses between two surfaces of two materials.