• ALGAE
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• v.35 no.2
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• pp.177-187
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• 2020

Cyanobacteria have been widely reported to produce a variety of UV-absorbing mycosporine-like amino acids (MAAs). Herein, we reported production of the unusual MAA, mycosporine-glycine-alanine (MGA) in the cyanobacterium Sphaerospermopsis torques-reginae ITEP-024 using a newly developed UHPLC-DAD-MS/HRMS (ultra-high performance liquid chromatography-diode array detection-high resolution tandem mass spectrometry) method. MGA had previously been first identified in a red-algae, but S. torques-reginae strain ITEP-024 is the first cyanobacteria to be reported as an MGA producer. Herein, the chemical structure of MGA is fully elucidated from one-dimensional / two-dimensional nuclear magnetic resonance and HRMS data analyses. MAAs are unusually produced constitutively in S. torques-reginae ITEP-024, and this production was further enhanced following UV-irradiance. It has been proposed that MAA biosynthesis proceeds in cyanobacteria from the pentose phosphate pathway intermediate sedoheptulose 7-phosphate. Annotation of a gene cluster encoded in the genome sequence of S. torques-reginae ITEP-024 supports these gene products could catalyse the biosynthesis of MAAs. However, addition of glyphosate to cultures of S. torques-reginae ITEP-024 abolished constitutive and ultra-violet radiation induced production of MGA, shinorine and porphyra-334. This finding supports involvement of the shikimic acid pathway in the biosynthesis of MAAs by this species.

• Transactions of Materials Processing
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• v.17 no.2
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• pp.124-129
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• 2008

Net shape forging technologies give many effects into the costs and qualities for the finished products. So, the studies to reduce the additional machining amount are very important in forging industry. Specially, there are two main topics in cold forging industry, such as, tool life and precision forging. In this study, new forging technique was proposed to eliminate the machining process for fixing up the length and improve the lead accuracy of gear. The luck-up hub is manufactured through many processes, such as upsetting, piercing and direct extrusion. The gear is formed in direct extrusion process; however, lead accuracy of the gear is over allowance limit. Therefore, the additional sizing process must be added. In this study, process design for closed-die forging of a lock-up hub used for a component of automobile transmission was made using three-dimensional finite element simulations, and the strain distributions and velocity distributions are investigated through the post processor. The rigid-plastic finite-element method for back pressure forging has been used in order to reduce development time and die cost. Using the FEM simulation, we found the optimum value of back pressure. The prototypes of lock-up hub parts were forged into the net-shape. In the experiment, lead precision of tooth are measured by the CCMM(Contact Coordinate Measuring Machine). The dimensional accuracy of forged part was improved up to the 40% when back press was applied.

• Computers and Concrete
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• v.25 no.1
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• pp.67-73
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• 2020

Traditionally used analytical approach to predict the fatigue failure of reinforced concrete (RC) structure is generally conservative and has certain limitations. The nonlinear finite element method (FEM) offers less expensive solution for fatigue analysis with sufficient accuracy. However, the conventional implicit dynamic analysis is very expensive for high level computation. Whereas, an explicit dynamic analysis approach offers a computationally operative modelling to predict true responses of a structural element under periodic loading and might be perfectly matched to accomplish long life fatigue computations. Hence, this study simulates the fatigue behaviour of RC beams with finite element (FE) assemblage presenting a simplified explicit dynamic numerical solution to show computer aided fatigue behaviour of RC beam. A commercial FEM package, ABAQUS has been chosen for this complex modelling. The concrete has been modelled as a 8-node solid element providing competent compression hardening and tension stiffening. The steel reinforcements are simulated as two-node truss elements comprising elasto-plastic stress-strain behaviour. All the possible nonlinearities are duly incorporated. Time domain analysis has been adopted through an automatic Newmark-β time incremental technique. The program consists of twelve RC beams to visualize the real behaviour during fatigue process and to obtain the reliability of the study. Both the numerical and experimental results indicate a redistribution of stresses along the time and damage accumulation of beam which severely affect the serviceability and ultimate capacity of RC beam. The output of the FEM analysis demonstrates good match with the experimental consequences which affirm the efficacy of the computer aided model. The controlled fatigue damage evolution at service fatigue load limits makes the FE model an efficient tool in predicting high cycle fatigue behaviour of RC structures.

• Journal of the Korean GEO-environmental Society
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• v.7 no.6
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• pp.89-100
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• 2006

Recently, lots of lives and properties have been lost because comparatively large magnitude earthquakes were occurred in relatively safe regions and nations. It has been reported that number of earthquakes was increased rapidly in Korea. Hence, recently civil constructions were ensured against risks about earthquake not only large-scale structures but also comparative small-scale structures such as reservoir dams and life line by systematic aseismic design. Therefore, in this study, the seismic stability was ensured to evaluate aseismic performance for major planned reservoir dams in Korea. The seismic response analyses were conducted using SHAKE program on new reservoir dams under short-period, long-period and artificial seismic wave. The liquefaction potential for reservoir dams was assessed by using results from seismic response analysis (simplified assessment method for liquefaction potential). Also, fully coupled analysis--interaction of pore-pressure and soil--was performed to investigate both the development of excess pore water pressure and the characteristic of dynamic shear strain.

• Korean Journal of Food Science and Technology
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• v.26 no.5
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• pp.479-483
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• 1994

The effects of the different protein source on serum cholesterol levels were studied in SD strain male rats. Fish protein prepared by the method of SUZUKI from Alaska Pollack (Theragra chalcogramma) was compared with casein and soybean protein isolate. Each protein source was incoporated into a cholesterol-free diet in order to provide a protein level of 20% for 2 weeks. The result obtained are as followed: Concentration of total-cholesterol, LDL-cholesterol, total-cholesterol/HDL-cholesterol and triglyceride in rats fed with fish protein group were significantly lower than those of rats fed with casein and similar to those of rats fed with soybean protein. In addition, it was shown that the ratio of Lys/Arg and Gly/Met+Cys of fish protein was close to that of soybean protein.

• YAKHAK HOEJI
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• v.56 no.4
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• pp.222-229
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• 2012

In order to determine acetylcholine and choline in the biological samples, the specific enzymes of acetylcholinesterase (AChE) and choline oxidase (ChO), which utilize acetylcholine and choline as substrates, were employed to convert substrates to $H_2O_2$. The produced $H_2O_2$ was coupled to 4-aminoantipyrine/phenol with peroxidase (PO) yielding quinoneimine dye which was measured at 508 nm. In the present enzymatic spectrophotometric analysis the product at the equilibrium state was measured considering accuracy, precision, time and cost of the analysis. The developed analytical method yielded good linearity (calibration curve; $A_{508}$=9534[acetylcholine]+0.009, correlation coefficient ($R^2$); 0.999) with detection limit of $1.11{\times}10^{-7}M$, reasonable precision (relative standard deviation; 0.10~1.62% at $2.5{\times}10^{-6}M{\sim}1.25{\times}10^{-4}M$) and accuracy (relative error; -0.24~0.97% at $4.13{\times}10^{-6}M{\sim}1.01{\times}10^{-4}M$) for acetylcholine chloride standard solution. The concentrations of acetylcholine and choline in human serum were found as $3.20{\times}10^{-5}M$ and $1.14{\times}10^{-4}M$, respectively. The brain tissues of Sprague-Dawley strain rat contained 9.82${\mu}g/g$ of acetylcholine and 6.53 ${\mu}g/g$ of choline in the cerebrum, while 7.37 ${\mu}g/g$ of acetylcholine and 5.34 ${\mu}g/g$ of choline in the cerebellum.

• Transactions of the Korean Society of Mechanical Engineers
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• v.13 no.3
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• pp.461-471
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• 1989

For the mixed-mode crack problems the direction of crack growth, the crack path and the rational representation of fatigue crack growth rates should be studied to predict fatigue life and safety of structures. In this study, a round specimen which produce nearly identical effects in all loading directions is proposed to make an easy measurement of initial direction of crack growth. The mode I and mode II stress intensity factors of the specimen were calculated using finite element method, in which the square root singular stresses at the crack tip are modeled by means of four rectangular quarter-point eight-noded elements surrounding the crack tip. Experimental results for high strength aluminum alloy showed that the direction of mixed-mode crack growth agree well with maximum principal stress criterion as well as minimum strain energy density criterion, but not with maximum shear stress criterion. From data of fatigue crack growth rates using crack geometry projected on the line perpendicular to the loading direction it is easily established that mixed-mode fatigue crack growth in 5083-H115 aluminum alloy goes predominantly with mode I crack growth behaviors.

• Journal of the Korean Society of Safety
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• v.10 no.3
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• pp.96-105
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• 1995

The better industry develops, the more spaces need but in the limited area. Most building become larger and more complicated if the more spaces need in the constant area. And this leads to do underground work in long period generally six(6) months for 6 basement stories due to the selection of TOP DOWN technique. Working environment in this underground area can be problems and should not be overlooked, because air quality in underground spaces become quickly worse. Recently, department name to control construction safety has been changed to ENVIRONMENT & SAFETY TEAM from SAFETY TEAM. This means that it is very important to control against environmental condition at site so much. Overall construction work as well as underground work should conform to the requirement of working environment, particularly against inhabitants around the construction area. Strut protection, one of earth protection method, in case to 40m long strut may become weaker due to thermal stress or its longitudinally compressive strain and the another one, earth anchor protection may not be applied to the site In case of encroaching on vertical underground borderline because of regulation to prohibit it. It is necessary that TOP DOWN technique should be introduced in order to solve the external and internal problem of the site such as difficulty level of the work, potential danger with excavating depth, and shortening workperiod. It is needed that improving way of working condition should be shown and simplified computer simulation program should be also provided for checking pollution level & ventilation, excluding of lighting problem here. Results measured with conformance to the Regulation for Working Environment Measurement, enforced by Ministry of Labor have been applied to the computer program developed here. Sample air taken at unit workplace which was considered as exposing condition of pollutant at breathing point and within a range of behavior of the workers, Identified exposing group in underground work, using Moded Flow Life Finally, three types of ventilation system, type I with blower & ventilator, type II natural supply with mechanical ventilation system, and type I mechanical ventilation with Drivent Fan Unit System are selected for this study.

• Structural Monitoring and Maintenance
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• v.2 no.3
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• pp.283-300
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• 2015

Visual condition inspections remain paramount to assessing the current deterioration status of a bridge and assigning remediation or maintenance tasks so as to ensure the ongoing serviceability of the structure. However, in recent years, there has been an increasing backlog of maintenance activities. Existing research reveals that this is attributable to the labour-intensive, subjective and disruptive nature of the current bridge inspection method. Current processes ultimately require lane closures, traffic guidance schemes and inspection equipment. This not only increases the whole-of-life costs of the bridge, but also increases the risk to the travelling public as issues affecting the structural integrity may go unaddressed. As a tool for bridge condition inspections, Unmanned Aerial Vehicles (UAVs) or, drones, offer considerable potential, allowing a bridge to be visually assessed without the need for inspectors to walk across the deck or utilise under-bridge inspection units. With current inspection processes placing additional strain on the existing bridge maintenance resources, the technology has the potential to significantly reduce the overall inspection costs and disruption caused to the travelling public. In addition to this, the use of automated aerial image capture enables engineers to better understand a situation through the 3D spatial context offered by UAV systems. However, the use of UAV for bridge inspection involves a number of critical issues to be resolved, including stability and accuracy of control, and safety to people. SLAM (Simultaneous Localisation and Mapping) is a technique that could be used by a UAV to build a map of the bridge underneath, while simultaneously determining its location on the constructed map. While there are considerable economic and risk-related benefits created through introducing entirely new ways of inspecting bridges and visualising information, there also remain hindrances to the wider deployment of UAVs. This study is to provide a context for use of UAVs for conducting visual bridge inspections, in addition to addressing the obstacles that are required to be overcome in order for the technology to be integrated into current practice.

• Journal of the Korea institute for structural maintenance and inspection
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• v.12 no.1
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• pp.101-108
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• 2008

This study is to examine bond strength of beam reinforced with GFRP rebar under 4-point fatigue bending test by adopting BRITISH STANDARD. The variables were made to have bonding length of 5times(5db), and 15times(15db) of the nominal diameter of GFRP rebar and were done to analyze the relationship between the bonding strength and the slip. In the result of the test, pull-out failure was dominant in the 5db specimen, patterns of the pull-out failure and concrete shear failure appeared in the 15db specimen showed only concrete shear failure at the end of bonding length. Therefore, The strain development consist of three different stage : A rapid increases form 0 to about 10% of total fatigue life. A uniform increases form 10% to about 70%~90%. Then a rapid increases until failure, if failure takes place. It seems that stress level has not influence on the secant modules of elasticity. And also according to the outcome the existing strengthening method came out to be the most superiority in S-N graphs.