• Journal of Plant Biotechnology
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• 제46권3호
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• pp.165-171
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• 2019

Marker-assisted backcrossing (MABC)은 marker-assisted selection (MAS)와 함께 다양한 작물에서 여교배 초기세대에서 반복친 게놈의 회복률이 높은 개체선발을 위한 분자육종 기술로 매우 유용하게 사용하고 있다. 본 연구에서는 토마토 MABC 육종 프로그램의 일환으로 저장성이 강한 rin유전자를 주)토마토연구소에서 육성한 핑크계 엘리트 토마토계통에 도입하고자 수행하였다. foreground 선발은 RIN SCAR 분자 마커를 이용하여 100개 $BC_1F_1$ 식물체에서 Rr 유전자형 가진 42개체를 선발하였다. 그리고 이를 이용하여 GBS 분석을 이용하여 background 선발을 하였다. 총 3,086개 SNP를 대상으로 반복친 HK13-1151과 게놈 회복률을 조사한 결과, 56.7%에서 84.5%를 보여 평균 70.5%로 나타났다. 이 중 87.2%을 보인 $BC_1F_1$개체를 이용하여 192개 $BC_2F_1$ 식물체를 육성하여 foreground 선발을 하였다. 선발된 102개 중 88개 식물체를 이용하여 GBS 분석을 수행한 결과 4,868개의 다형 SNP 마커를 얻었으며, 이를 이용하여 RPG 회복률을 조사하였다. $BC_2F_1$ 식물체들에서 HK13-1151 반복친 게놈과 87.8%에서 97.8% 유사하였다. 본 연구에서 $BC_2F_1$ 식물 중 RPG 회복률이 97.8%인 5-1 개체는 반복친인 HK13-1151과 과일특성에서 매우 유사하였다. 따라서 선발된 5-1 개체는 $BC_2F_2$ 세대를 육성하여 계통화 하고자 한다. 본 연구를 통해 MABC는 전통 여교배 육종에 비해 육종연한을 획기적으로 줄일 수 있으며, 원하는 육종모델을 완수할 수 있는 첨단육종 기술로 평가 할 수 있다.

• Steel and Composite Structures
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• 제35권6호
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• pp.799-813
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• 2020

Fatigue cracks of rib-to-deck (RD) joints have been frequently observed in the orthotropic steel decks (OSD) using conventional U-ribs (CU). Thickened edge U-rib (TEU) is proposed to enhance the fatigue strength of RD joints, and its effectiveness has been proved through fatigue tests. In-depth full-scale tests are further carried out to investigate both the fatigue strength and fractography of RD joints. Based on the test result, the mean fatigue strength of TEU specimens is 21% and 17% higher than that of CU specimens in terms of nominal and hot spot stress, respectively. Meanwhile, the development of fatigue cracks has been measured using the strain gauges installed along the welded joint. It is found that such the crack remains almost in semi-elliptical shape during the initiation and propagation. For the further application of TEUs, the design curve under the specific survival rate is required for the RD joints using TEUs. Since the fatigue strength of welded joints is highly scattered, the design curves derived by using the limited test data only are not reliable enough to be used as the reference. On this ground, an experiment-numerical hybrid approach is employed. Basing on the fatigue test, a probabilistic assessment model has been established to predict the fatigue strength of RD joints. In the model, the randomness in material properties, initial flaws and local geometries has been taken into consideration. The multiple-site initiation and coalescence of fatigue cracks are also considered to improve the accuracy. Validation of the model has been rigorously conducted using the test data. By extending the validated model, large-scale databases of fatigue life could be generated in a short period. Through the regression analysis on the generated database, design curves of the RD joint have been derived under the 95% survival rate. As the result, FAT 85 and FAT 110 curves with the power index m of 2.89 are recommended in the fatigue evaluation on the RD joint using TEUs in terms of nominal stress and hot spot stress respectively. Meanwhile, FAT 70 and FAT 90 curves with m of 2.92 are suggested in the evaluation on the RD joint using CUs in terms of nominal stress and hot spot stress, respectively.