• Research in Plant Disease
• /
• v.29 no.4
• /
• pp.445-451
• /
• 2023

Zamioculcas zamiifolia is a popular indoor ornamental plant in Korea. In August 2021, a severe outbreak of soft rot disease affected Z. zamiifolia in Emseong, Chungcheongbuk-do, Korea. Infected plants displayed wilting, water-soaked lesions, stem collapse, and green-brown discoloration. The bacterial strain KNUB-05-21 was isolated from infected stems and identified as Pectobacterium aroidearum using 16S rRNA nucleotide sequencing and multilocus sequence analysis based on partial sequences of dnaX, leuS, and recA genes. Confirmation of its affiliation with P. aroidearum was also obtained through biochemical and morphological characterization. To confirm the pathogenicity of strain KNUB-05-21, its suspension was injected into Z. zamiifolia stems. Within a week, soft rot developed on the stems, exhibiting symptoms similar to those observed in field-infected plants. The reisolated strain was identical to those of P. aroidearum. Before this study, P. aroidearum was not reported as a causative pathogen of Z. zamiifolia soft rot in Korea.

• Journal of Mechanical Science and Technology
• /
• v.20 no.1
• /
• pp.42-50
• /
• 2006

To evaluate the fatigue lifetime of structures, it is necessary to identify the values of parameters through tests. From the viewpoint of time and cost it is difficult for engineers to get the necessary data through tests. In this study, we surveyed literature and proposed a procedure to identify the fatigue parameters expressed with the Brinell hardness and elastic modulus. After obtaining stress concentration factors by finite element analysis, we calculated fatigue notch factors using Peterson's formula. Taking into account the welding residual stress, which was also obtained by finite element analysis, we evaluated the fatigue lifetime of four kinds of welded joints using the proposed approach. The estimated results are in a good agreement with the experimental results.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.3 no.2
• /
• pp.112-121
• /
• 1995

Various CAE technologies are used in automobile industries for the purpose of design and analysis. In this paper, a fatigue life evaluation system FLEVA based on the local strain approach is developed and the system is applied for the fatigue strength design of the suspension knuckle, an automobile component. Various steps such as material test, finite element analysis and cumulative fatigue damage analysis of the suspension knuckle were taken. The usefulness of the approach was verified by the fatigue test on the suspension knuckle.

• Mycobiology
• /
• v.42 no.1
• /
• pp.46-51
• /
• 2014

A degenerated strain of Pleurotus eryngii KNR2312 was isolated from a commercial farm. Random amplified polymorphic DNA analysis performed on the genomic DNA of the normal and degenerated strains of this species revealed differences in the DNA banding pattern. A unique DNA fragment (1.7 kbp), which appeared only in the degenerated strain, was isolated and sequenced. Comparing this sequence with the KNR2312 genomic sequence showed that the sequence of the degenerated strain comprised three DNA regions that originated from nine distinct scaffolds of the genomic sequence, suggesting that chromosome-level changes had occurred in the degenerated strain. Using the unique sequence, three sets of PCR primers were designed that targeted the full length, the 5' half, and the 3' half of the DNA. The primer sets P2-1 and P2-2 yielded 1.76 and 0.97 kbp PCR products, respectively, only in the case of the degenerated strain, whereas P2-3 generated a 0.8 kbp product in both the normal and the degenerated strains because its target region was intact in the normal strain as well. In the case of the P2-1 and P2-2 sets, the priming regions of the forward and reverse primers were located at distinct genomic scaffolds in the normal strain. These two primer sets specifically detected the degenerate strain of KNR2312 isolated from various mushrooms including 10 different strains of P. eryngii, four strains of P. ostreatus, and 11 other wild mushrooms.

• Mycobiology
• /
• v.44 no.4
• /
• pp.283-290
• /
• 2016

A double-stranded RNA (dsRNA) mycovirus was detected in malformed fruiting bodies of Pleurotus ostreatus strain ASI2792, one of bottle cultivated commercial strains of the edible oyster mushroom. The partial RNA-dependent RNA polymerase (RdRp) gene of the P. ostreatus ASI2792 mycovirus (PoV-ASI2792) was cloned, and a cDNA sequences alignment revealed that the sequence was identical to the RdRp gene of a known PoSV found in the P. ostreatus strain. To investigate the symptoms of PoV-ASI2792 infection by comparing the isogenic virus-free P. ostreatus strains with a virus-infected strain, isogenic virus-cured P. ostreatus strains were obtained by the mycelial fragmentation method for virus curing. The absence of virus was verified with gel electrophoresis after dsRNA-specific virus purification and Northern blot analysis using a partial RdRp cDNA of PoV-ASI2792. The growth rate and mycelial dry weight of virus-infected P. ostreatus strain with PoV-ASI2792 mycovirus were compared to those of three virus-free isogenic strains on 10 different media. The virus-cured strains showed distinctly higher mycelial growth rates and dry weights on all kinds of experimental culture media, with at least a 2.2-fold higher mycelial growth rate on mushroom complete media (MCM) and Hamada media, and a 2.7-fold higher mycelial dry weight on MCM and yeast-malt-glucose agar media than those of the virus-infected strain. These results suggest that the infection of PoV mycovirus has a deleterious effect on the vegetative growth of P. ostreatus.

• Journal of Life Science
• /
• v.22 no.12
• /
• pp.1718-1723
• /
• 2012

Ten endophytic fungi with different colony morphologies were isolated from the roots of Suaeda japonica Makino growing naturally in Suncheon Bay. Plant growth promotion was verified by treating waito-c rice seedlings with culture filtrates from the endophytic fungi. The bioassays showed that the Sj/7/4 fungal strain induced effective growth promotion in the seedlings. The gibberellins (GA) produced by fungal strain Sj/7/4 were analyzed by gas chromatography /mass spectroscopy with selected ion monitoring (GC/MS SIM). The culture filtrate of Sj/7/4 fungal strain was confirmed to contain $GA_4$ through quantitative analysis. The Sj/7/4 fungal strain was identified to determine the internal transcribed spacer (ITS) regions with universal primers ITS-1 and ITS-4 by using polymerase chain reactions (PCR). Molecular analysis of the Sj/7/4 fungal strain showed high similarity to Fusarium solani. The Sj/7/4 fungal strain isolated from the root of S. japonica was therefore designated as F. solani Sj/7/4.

• Journal of the Korean Society for Precision Engineering
• /
• v.14 no.12
• /
• pp.73-85
• /
• 1997

The rolling force and roll deformation behavior in the twin roll type strip continuous casting process has been computed to estimate the thermal charcteristics of a caster roll. To calculation of rolling force, the relationship between flow stress and strain for a roll material and casting alloy are assumed as a function of strain-rate and temperature because mechanical properties of a casting materials depends on tempera- ture. The three dimensional thermal dlastic-plastic analysis of a cooling roll has also been carried out to obtain a roll stress and plastic strain distributions with the commercial finite element analysis package of ANSYS. Temperature fields data of caster roll which are provided by authors were used to estimated of roll deformation. Roll life considering thermal cycle is calculated by using thermal elastic-plastic analysis results. Roll life is proposed as a terms of a roll revolution in the caster roll with and without fine failure model on the roll surface. To obtain of plastic strain distributions of caster roll, thermomechan- ical properties of roll sleeve with a copper alloy is obtained by uniaxial tensile test for variation of temperature.

• Advances in aircraft and spacecraft science
• /
• v.4 no.1
• /
• pp.1-19
• /
• 2017

Fatigue life prediction of a multi-row countersunk riveted lap joint was performed numerically. The stress and strain conditions in a highly stressed substructure of the joint were analysed using a global/local finite element (FE) model coupling approach. After validation of the FE models using experimental strain measurements, the stress/strain condition in the local three-dimensional (3D) FE model was simulated under a fatigue loading condition. This local model involved multiple load cases with nonlinearity in material properties, geometric deformation, and contact boundary conditions. The resulting stresses and strains were used in the Smith-Watson-Topper (SWT) strain life equation to assess the fatigue "initiation life", defined as the life to a 0.5 mm deep crack. Effects of the rivet-hole clearance and rivet head deformation on the predicted fatigue life were identified, and good agreement in the fatigue life was obtained between the experimental and the numerical results. Further crack growth from a 0.5 mm crack to the first linkup of two adjacent cracks was evaluated using the NRC in-house tool, CanGROW. Good correlation in the fatigue life was also obtained between the experimental result and the crack growth analysis. The study shows that the selected methodology is promising for assessing the fatigue life for the lap joint, which is expected to improve research efficiency by reducing test quantity and cost.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.40 no.12
• /
• pp.1027-1035
• /
• 2016

In this study, a strain-controlled fatigue test of widely-used 316L stainless steel with excellent corrosion resistance and mechanical properties was conducted, in order to assess its fatigue life. Low cycle fatigue behaviors were analyzed at room temperature, as a function of the strain amplitude and strain ratio. The material was hardened during the initial few cycles, and then was softened during the long post period, until failure occurred. The fatigue life decreased with increasing strain amplitude. Masing behavior in the hysteresis loop was shown under the low strain amplitude, whereas the high strain amplitude caused non-Masing behavior and reduced the mean stress. Low cycle fatigue life prediction based on the cyclic plastic energy dissipation theory, considering Masing and non-Masing effects, showed a good correlation with the experimental results.

• ALGAE
• /
• v.37 no.4
• /
• pp.317-329
• /
• 2022

Carotenoids are effective antioxidants that are found in various photosynthetic organisms. Marine microalgae are an advantageous bioresource for carotenoid production because they do not compete with other crops for freshwater and arable land. This study reports a newly isolated Dunaliella strain from the Geumhong Saltpan on Yeongjong Island, West Sea, Korea. The new strain was isolated and classified as Dunaliella salina through phylogenetic analysis and was named the OH214 strain (Deposit ID: KCTC14434BP). The newly isolated strain can survive in a wide range of NaCl concentrations (0.3-5.0 M NaCl), but grows well in 0.6 to 1.5 M NaCl culture medium. Under high-light conditions (500 ± 10 μmol photons m^-2 s^-1), the cells accumulated three times more β-carotene than under low-light conditions (50 ± 5 μmol photons m^-2 s^-1). The cells accumulated 2.5-fold more β-carotene under nitrogen-deficient (1 mM KNO₃) conditions (3.24 ± 0.36 ㎍ 10⁶ cells^-1) than in nitrogen-sufficient conditions (>5 mM KNO₃). The lutein content under nitrogen-deficient conditions (1.73 ± 0.09 ㎍ 10⁶ cells^-1) was more than 24% higher than that under nitrogen-sufficient conditions. Under the optimized culture condition for carotenoid induction using natural seawater, D. salina OH214 strain produced 7.97 ± 0.09 mg g DCW^-1 of β-carotene and 4.65 ± 0.18 mg g DCW^-1 of lutein, respectively. We propose that this new microalga is a promising strain for the simultaneous production of β-carotene and lutein.