• 한국식품과학회지
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• 제28권6호
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• pp.1142-1145
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• 1996

수확 후 감귤의 장기저장은 주로 곰팡이에 의한 부패로 어려움을 겪어왔다. 본 연구에서는 감귤부패 곰팡이를 수집, 동정함으로써 수확후 저장성을 증대하는데 기여하고자 하였다. 1994년 가을부터 1995년 봄에 걸쳐 대기중에 노출된 채로 상온에 저장 중 부패된 온주밀감으로부터 14개의 상이한 균주를 분리하였다. 저장 중 각 곰팡이에의한 병해의 발생빈도는 Penicillium italicum 25.8%, Monila candida 19.8%, Alternaria citri 18.1%, Mucor hiemalis 11.0%, Phomopsis citri 6.6%, Botrytis cinerea 5.5%, Phoma citricarpa 3.8%, Glomerella cingulata 3.8% 그리고 P. digitatum 1.1% 순이었고, 기타 곰팡이가 4.5%를 차지 하였다. 한편 생리적 특성상 동정된 모든 균주가 펙틴 분해효소를 생산하였다. 이러한 연구결과는 감귤의 부패와 곰팡이의 펙틴분해능간에는 연관성이 있음을 시사한다. 수집된 곰팡이 균주는 감귤의 부패를 방지하는 연구에 이용될 것이다.

• 한국응용곤충학회지
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• 제42권4호
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• pp.329-334
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• 2003

쌀바구미와 저장 곡물 곰팡이간의 상호작용 분석의 일환으로 곰팡이 종에 대한 쌀바구미의 분포 선호성을 조사하였다. 쌀바구미는 비교 대상 4종 곰팡이(Aspergillus candidus, A. niger, A. flavus와 Penicillium spp.)에 오염된 현미를 살균된 현미보다 선호하였으며, 선호도는 A. candidus와 Penicillium spp.에 대해서 가장 높았다. 차대 쌀바구미 우화수는 A. candidus와 Penicillium 오염미에서 가장 많았으며 A. flavus 오염미에서 가장 적었다. 쌀바구미의 곰팡이 오염미에 대한 선호와 쌀바구미 적합도간 불일치 현상은 쌀바구미의 분포 및 곡물 곰팡이 선호도가 알려진바 유사 니체에서의 장기적인 공진화 과정을 거쳐 이루어진 종간 상호작용의 조정과 아울러 영양적 특성 등 단기적 서식환경의 적절성의 두 가지 측면에서 설명될 수 있음을 암시하는 것으로 생각된다.

• 한국식품영양과학회지
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• 제28권1호
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• pp.40-46
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• 1999

Microbiological quality of commercial white ginseng was examined, together with investigation on its water absorption pattern and radiosensitivity of molds isolated from the samples. Comparative effects of phosphine fumigation and gamma irradiation on microbial control and disinfestation of the samples packed in a commercial laminated film and stored for six months at ambient(20oC, 70% RH) and accelerated(40oC, 90% RH) conditions were studied. Commercial white ginseng was contaminated with coliforms by 103~104CFU/g and molds by 102~104CFU/g and thus decontamination process was required for hygienic quality and storage stability. Phosphine fumigation showed no sterilizing effects on microbes contaminated, while gamma irradiation at around 5kGy was effective for decontaminating microorganisms, showing D10 values of 0.48~0.60kGy for isolated molds. Even though the storage insects, Plodia interpuctella Hubner and Lasioderma serricorne(cigarette beetle) were easily destroyed by phosphine fumigation, gamma irradiation less than 5kGy was found effective for both purposes to improve biological quality of stored white ginseng, thereby extending the storage life of packed samples resulting from increased critical moisture content by about 1%(Aw 0.76).

• Journal of Applied Biological Chemistry
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• 제50권4호
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• pp.239-243
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• 2007

Effects of aqueous chlorine dioxide ($ClO_2$) treatment on the microbial growth and the quality of iceberg lettuce during storage were examined. Lettuce samples were treated with 0, 5, 10, and 50 ppm of $ClO_2$ solution and stored at $4^{\circ}C$. Aqueous $ClO_2$ treatment significantly decreased the populations of total aerobic bacteria, yeasts and molds, and coliforms on the shredded lettuce. Fifty ppm $ClO_2$ treatment reduced the initial populations of total aerobic bacteria, yeasts and molds, and coliforms by 1.77, 1.34, 1.10 log CFU/g, respectively. Aqueous $ClO_2$ treatment caused negligible changes in the Hunter color L, a, and b values during storage. Sensory evaluations exhibited that there were no significant changes among treatments. These results indicate that the aqueous $ClO_2$ treatment can be useful in improving the microbial safety of the iceberg lettuce during storage and extending the shelf life.

• Food Science and Biotechnology
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• 제18권3호
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• pp.636-640
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• 2009

The effect of post-cut vanillin treatment at high concentrations on changes of quality and microorganism in fresh-cut apples was studied. Apples (Malus domestica Borkh. cv. Fuji) were sliced, treated by dipping in different vanillin solution, 0, 40, 80, and 120 mM, packed in polyethylene bag, and then stored for up to 3 weeks at $4^{\circ}C$. Changes in total aerobic bacteria, yeast and molds, browning, soluble solids, and titratable acidity during storage were investigated. Growth of total aerobic bacteria throughout storage was strongly inhibited by vanillin regardless of treatment concentrations. Growth of yeast and molds was inhibited by vanillin of all concentrations until 2 weeks of storage. Levels of browning index, soluble solids, and titratable acidity were not significant difference among the treatment conditions until 2 weeks of storage. However, when stored for 3 weeks, browning index increased more at 80 or 120 mM vanillin, while soluble solids and titratable acidity more be decreased by 120 mM vanillin as compared with other treatment conditions. These results show that the usage of vanillin in processing of fresh-cut apples had a limitation for maintaining quality.

• Design & Manufacturing
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• 제14권2호
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• pp.1-6
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• 2020

Recently, research and commercialization related to the field of cell-based therapeutic drug development has been actively conducted. In order to maintain cell viability and prevent contamination, refrigeration preservation devices, such as CRF (controlled rate freezer) or vapor type LN2 tanks have been developed. On the other hand, the storage container for liquid nitrogen tanks currently on sale minimizes the flow structure to prevent structural defects when stored in a liquid nitrogen tank having a high thermal conductivity than vapor nitrogen. If the cell-based treatment drug is stored in the gaseous LN2 tank as it is, the cell survival after thawing is greatly reduced. It was estimated that the existing storage container structure was a factor that prevented the rapid entry and circulation of gaseous nitrogen into the container. Therefore, this study intends to propose a new supercellular storage container model that can maintain the mechanical strength while maximizing the fluid flow structure. To this end, we estimated that the structural change of the storage container effects on the equivalent stress formed around the through-holes of them when exposed to a cryogenic environment using thermal-structural coupled field analysis. As a result of storage experiments in the gas phase tank of the cell-based therapeutic agent using the developed storage container, it was confirmed that the cell growth rate was improved from 66% to 77%, which satisfied the transportation standards of the FDA(Food and Drug Administration) cell-based therapeutic agent.

• 한국응용곤충학회지
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• 제13권2호
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• pp.71-75
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• 1974

1. 쌀바구미류는 성충>유충>용의 순으로 체내에 곰팡이를 많이 갖고 있었다. 2. 무균소맥에서 사육하면서 체내 균류의 잔존상황을 조사한 바 10일후에는 완전히 제거할 수 있었고, 가장 오래 잔류하는 것은 A. candidus였다. 3. 곰팡이류의 산난수에 미치는 영향은 A. candidus 구가 가장 많았고 A. ruber 그리고 대조구의 순이고 A. niger구가 가장 적었다. 4. 유충의 생육은 A. candidus>A. ruber>대조구의 순으로 양호하였고 A. niger구에서는 2-3령까지 밖에 발육을 하지 못하였다. 5. 생육이 가장 빠른 것은 A. candidus구로 제3주에용화개체를 볼 수 있었고 제4편에 성충 우화를 볼 수 있었다. 대조구에서도 동일한 경향을 볼 수 있었으나 생육은 약간 늦었다. 6. A. niger구에서 생육이 불량한 것은 왕성한 균생장으로 쌀바구미의 활동이 저해되는 것과 동시에 불명의 원인이 있는 듯 하였다. 7. 저곡중의 쌀바구미와 곰팡이류간에는 진화과정을 통하여 원시적 협동작용이 있었다.

• 한국식품위생안전성학회지
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• 제10권2호
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• pp.103-109
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• 1995

Microbiological and organoleptic qualities of boiled anchovies were evaluated during storage for 8 months at different temperatures and packaging methods. Microbial populations of marketing samples were 106~107 CFU/g in aerobic bacteria, 103~106 CFU/g in yeasts/molds and 103~105 CFU/g in coliforms, respectively, which were the highest in retail sample, followed by in military goods and wholesale sample. Moreover, anchovies supplied for retail sale and military goods were contaminated with sanitary indicative microbes. The samples stored at ambient condition(15~33$^{\circ}C$, RH 5$0^{\circ}C$95%) lost their marketable quaity mainly due to microbial propagation prior to 6 months, irrespective of packaging methods, corrugated-cardboard box and laminated-film(nylon 15${\mu}{\textrm}{m}$/PE 100${\mu}{\textrm}{m}$). However, cooling(5~1$0^{\circ}C$) as well as well as freezing temperatures($\leq$-18$^{\circ}C$) following laminated-film packaging were effective for keeping the organoleptic qualities of stored anchovies up to 8 months. The population of yeasts and molds was shown the quality-indicative criteria for stored anchovies and their critical levels were 5.00 log counts/g, showing a higher negative-correlationship(r=-0.901) with changes in organoleptic quality.

• 한국포장학회지
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• 제28권1호
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• pp.9-14
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• 2022

Packages of different atmospheres (air (control), 100% CO₂, vacuum, and vacuum + O₂ absorber) were prepared for 0.4 kg rice cake (ddukgukdduk) using gas-barrier plastic film and stored at 10℃ for 11 days. The stored products were evaluated in their packages atmosphere, total aerobic bacteria, yeast and molds, texture and sensory quality during storage period. In the air package, the O₂ concentration decreased from initial 21% to 16% on storage 4 days and the CO₂ concentration increased to 23% on storage of 11 days, which resulted from the growth of microorganisms. CO₂ concentration decreased from initial 98% to 36% on storage 11 days in the 100% CO₂ package. It is reasoned that CO₂ was dissolved into the product reducing the volume of the package. Vacuum and vacuum +O₂ absorber package maintained shrunk vacuum condition until 11 days of storage. Total aerobic bacteria count increased significantly in the control package (6.41 log (cfu/g) after 11 days) compared to the 100% CO₂ package (4.96 log (cfu/g) after 11 days). Yeast and molds were 6.66 in control package, 3.43 in 100% CO₂ package, 4.66 in vacuum package, and 3.78 in vacuum + O₂ absorber package after 11 days. There was no significant difference between control and the other treatments for the texture of the stored products. Sensory quality was the worst in control package on the storage of 8 days. All treatment groups except control improved the quality preservation, but vacuum and vacuum + O₂ absorber packages suffered from cracking of the product. Thus 100% CO₂ flushing is suggested as a desired packaging condition.

• Applied Biological Chemistry
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• 제42권4호
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• pp.356-360
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• 1999

감귤저장 방법의 개선과 감귤 껍질의 효율적인 활용을 위해서 시판 중인 농약제제와 감귤정유의 감귤부패 관련 사상균에 대한 살균 작용을 조사하였다. Alternaria alternata, Rhizopus sp., Botrytis cinerea, Monilia candida, Penicillium italicum, Penicillium digitatum 등 6종의 사상균에 대한 시판 합성 농약제제 11종의 생육저해 효과를 조사한 결과, mancozeb 또는 fluazinam을 함유한 제제의 활성 범위가 가장 넓었고, conazole 계통의 화합물이 중간 정도의 활성 범위를 나타내었으며, iprodione, benomyl, azoxtrobin 또는 thiophanate을 함유한 제제의 항균활성 범위가 가장 좁은 것으로 나타났다. 농약제제의 생육저해 효과는 대상 균주에 따라 달랐으며, Penicillium italicum과 Alternaria alternata는 각종 살균제의 처리에 대한 생존력이 비교적 강하였다. 감귤정유를 상기 6개 균주의 포자와 각각 접촉시켰을 때는 그 어느 균주의 생존 포자도 검출되지 않았으며, 감귤정유의 살균력은 paper disk법과 현미경 관찰로도 증명되었다. 이는 감귤정유가 광범위한 살균력을 지니고 있음을 제시한다. 따라서 감귤 정유를 감귤저장에 활용하는 방법을 검토할 필요가 있다.