• The Korean Journal of Physiology
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• v.24 no.2
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• pp.331-344
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• 1990

What makes glucose transport function sensitive to insulin in one cell type such as adipocyte, and insensitive in another such as liver cells is unresolved question at this time. Recently it is known that insulin stimulates glucose transport in adipocytes largely by redistributing transporter from the storage pool that is included in a low density microsomal fraction to plasma membrane. Therefore, insulin sensitivity may depend upon the relative distribution of gluscose transporters between the plasma membrane and in an intracellular storage compartment. In hepatocytes, the subcellular distribution of glucose transporter is less well documented. It is thus possible that the apparent insensitivity of the hepatocyte system could be either due to lack of the constitutively maintained, intracellular storage pool of glucose transporter or lack of insulin-mediated transporter translocation mechanism in this cell. In this study, I examined if any intracellular glucose transporter pool exists in hepatocytes and this pool is affected by insulin. The results obtained summarized as followings: 1) Distribution of subcellular fractions of hepatocyte showed that there are $24.9{\pm}1.3%$ of plasma membrane, $36.9{\pm}1.7%$ of nucleus-mitochondria enriched fraction, $23.5{\pm}1.2%$ of lysosomal fraction, $9.6{\pm}1.0%$ of high density microsomal fraction and $4.9{\pm}0.5%$ of low density microsomal fraction. 2) In adipocyte, there were $29.9{\pm}2.6%$ of plasma membrane, $19.4{\pm}1.9%$ of nucleus-mitochondria enriched fraction, $26.7{\pm}1.8%$ of high density microsomal fraction and $23.9{\pm}2.1%$ of low density microsomal fraction. 3) Surface labelling of sodium borohydride revealed that plasma membrane contaminated to lysosomal fraction by $26.8{\pm}2.8%$, high density microsomal fraction by $8.3{\pm}1.3%$ and low density microsomal fraction by $1.7{\pm}0.4%$ respectively. 4) Cytochalasin B bound to all of subcellular fractions with a Kd of $1.0{\times}10^{-6}M$. 5) Photolabelling of cytochalasin B to subcellular fractions occurred on 45 K dalton protein band, a putative glucose transporter and D-glucose inhibited the photolabelling. 6) Insulin didn't affect on the distribution of subcellular fractions and translocation of intracellular glucose transporters of hepatocytes. 7) HEGT reconstituted into hepatocytes was largely associated with plasma membrane and very little was found in low density microsomal fraction which equals to the native glucose transporter distribution. Insulin didn't affect on the distribution of exogeneous glucose transporter in hepatocytes. From the above results it is concluded that insulin insensitivity of hepatocyte may due to lack of intracellular storage pool of glucose transporter and thus intracellular storage pool of glucose transporter is an essential feature of the insulin action.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.6
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• pp.829-834
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• 1998

It is well known that the native conformation of many proteins can be stabilized by carbohydrates or polyalcohols. However, the mechanism of the stabilization still remains unclear. In the present studies, to characterize the cryoprotective mechanism of corn starch enzyme hydrolysates on fish protin, solubility of hydrolysates, thermal behavior of hydrolysates and actomyosin solution, and enzyme kinetics in frozen system were investigated. The solubility of the hydrolysates increased with the increase in D.E. value. The $T_g^{'}$ of the hydrolysates were linearly correlated with D.E. value and the T-g value of the hydrolysates (D.E. 5,10,15,20) were reported to be $-7.2^{\circ}C\;-8.8^{\circ}C\;-11.9^{\circ}C$, and $-14.3^{\circ}C$, respectively. The results of enzyme experiments showed that the higher the D.E. value, the higher was the rate of reaction in frozen storage ($-12^{\circ}C$). It is found to support the cryostabilization mechanism that the hydrolysats act to enmesh the protein in a glass state where all deteriorative processes are greatly slowed down.

• Proceedings of the IEEK Conference
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• 2000.06c
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• pp.97-100
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• 2000

As network resources have become faster and demands for multimedia service through network have increased, the demand for Media server system has increased. These kinds of media server solve their bottle neck problem of internal storage device by using parallel system which takes advantage of fast network resource. Many vendors have suggested each of their media server system to solve these problem radically, but most of them require major modification of infra component and additional drawback has added. For example, storage mechanism for specific media requires new file system which is totally different from traditional one, and algorithm for enhancing performance may not suit for traditional operating system environment. In this paper, we designed a parallel media server based on web interface of traditional system and implemented a program for media server. Implemented server system performs parallel processing through web interface without any modification of traditional system, and controls which is related to merging load by distributed data is charged only to client and control server and consequently load of storage server can be minimized. And also, data transfer protocol for streaming media includes Retransfer algorithm and client Admission control policy relevant to performance of whole system.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2002.05a
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• pp.357-360
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• 2002

Nowadays, the improvement and development of Multi-media, information and communication technology are rapidly processed. And many products, for example, digital camera, digital camcorder, and PDA, are used for them. They need large data storage capacity and small size, light storage system. Due to that, many studies and researches in data storage system have been carried out. Especially, micro drive system was presented by IBM.(1) However, its system is expensive and uneasy to be portable. In ODD technologies, 1 inch drive system is not yet or in processing status.(2) If to be possible and to be come up, it is cheap than HDD system and easy to transfer information. In this paper, a miniaturized actuator(about linch) is designed and tested for ODD system. Specially, it is adapted for NFR(Near-field Recoding) system using SIL(Solid Immersion Lens). It is the dual-servo actuator which consists of a coarse actuator and fine actuator. Its actuating force generation method is VCM(Voice Ceil Motor). The fine actuator has 4-wire suspensions and bobbin wrapped by coil and includes focusing motion as well as tracking motion. The coarse actuator has an actuating coil and V-grooved guide mechanism. Also, the characteristics of the designed actuator is estimated by sine-swept mode and LDV(Laser Doppler Vibro-meter).

• Journal of the Korean Society of Industry Convergence
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• v.3 no.2
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• pp.167-178
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• 2000

Software RAID(Redundant Arrays of Inexpensive Disks) is defined as a storage system that provides capabilities of hardware RAID, and guarantees high reliability as well as high performance. In this paper, we propose an enhanced disk scheduling algorithm and a scheme to guarantee reliability of data. We also design and implement software RAID by utilizing these mechanism to develop a storage system for multimedia applications. Because the proposed algorithm improves a defect of traditional GSS algorithm that disk I/O requests arc served in a fixed order, it minimizes buffer consumption and reduces the number of deadline miss through service group exchange. Software RAID also alleviates data copy overhead during disk services by sharing kernel memory. Even though the implemented software RAID uses the parity approach to guarantee reliability of data, it adopts different data allocation scheme. Therefore, we reduce disk accesses in logical XOR operations to compute the new parity data on all write operations. In the performance evaluation experiments, we found that if we apply the proposed schemes to implement the Software RAID, it can be used as a storage system for small-sized video-on-demand servers.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.11
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• pp.5653-5672
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• 2019

In cloud computing era, an increasing number of resource-constrained users outsource their data to cloud servers. Due to the untrustworthiness of cloud servers, it is important to ensure the integrity of outsourced data. However, most of existing solutions still have challenging issues needing to be addressed, such as the identity privacy protection of users, the traceability of users, the supporting of dynamic user operations, and the publicity of auditing. In order to tackle these issues simultaneously, in this paper, we propose a traceable dynamic public auditing scheme with identity privacy preserving for cloud storage. In the proposed scheme, a single user, including a group manager, is unable to know the signer's identity. Furthermore, our scheme realizes traceability based on a secret sharing mechanism and supports dynamic user operations. Based on the security and efficiency analysis, it is shown that our scheme is secure and efficient.

• Applied Biological Chemistry
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• v.32 no.1
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• pp.64-72
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• 1989

Near full length cDNA clones encoding the rice seed storage protein, prolamine, were isolated and divided into two homology classes based on cross-hybridization and DNA sequencing analysis. These cDNA clones contain a single open reading frame encoding a putative rice prolamine precursor(M.W.=17,200) possessing atypical 14 amino acid signal peptide. Clones of these two homology classes diverge mainly by insertions/deletions of short nucleotide stretches and point mutations. The deduced primary structures of both types of prolamine polypeptides are devoid of any major tandem repetitive sequences, a feature prevalent in other cereal prolamines. No significant homology teas detected between the rice prolamine and other cereal prolamines, indicating that the rice gene evolved from a different ancestor that gave rise to other cereal prolamine genes. Developing wheat and rice endosperms were examined using ultrathin sections prepared from tissues harvested at various days after flowering. By immunocytochemical localization techniques, wheat prolamines are localized within vesicles from Golgi apparatus and in homogeneous regions of protein bodies. The involvement of the goli apparatus in the packaging of wheat prolamines into protein bodies indicates a pathway which differs from the mode of other cereal prolamines and resembles the mechanism employed for the storage of rice glutelin and legume globulins.

• Journal of Biosystems Engineering
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• v.26 no.4
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• pp.385-390
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• 2001

The Ondol system using both air-to-water heat pump and PCM(Phase Change Material) was constructed, and the effects of ambient air temperature on COP(Coefficient of Performance) of heat pump, the amount of heat supplied to the Ondol in the heating process, the heat storage in the PCM and the variation of Ondol room temperature were analyzed. The results from this study could be summarized as follows: 1. The COP of the heat pump (3 PS) was in proportion to the ambient air temperature. 2. When the ambient air temperature was varied between -10$^{\circ}C$ and -7$^{\circ}C$, the air temperature in the Ondol room was maintained between 16$^{\circ}C$ and 22$^{\circ}C$. As the results, it was certified that the heat pump-latent heat storage type Ondol system could be a comfortable residential heating system in the winter. 3. The maximum radiation and convection heat transfer from Ondol surface was 206.2 kJ/㎥hr and 82.6 kJ/㎥hr respectively. As the results, it could be confirmed that the radiation was major heat transfer mechanism for the Ondol room heating.

• Journal of the Korea Society for Simulation
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• v.11 no.3
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• pp.23-34
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• 2002

Dynamic storage allocation (DSA) is a field fairly well studied for a long time as a basic problem of system software area. Due to memory fragmentation problem of DSA and its unpredictable worst case execution time, real-time system designers have believed that DSA may not be promising for real-time application service. Recently, the need for an efficient DSA algorithm is widely discussed and the algorithm is considered to be very important in the real-time system. This paper proposes an efficient DSA algorithm called QSB (quick semi-buddy) which is designed to be suitable for real-time environment. QSB scheme effectively maintains free lists based on quick-fit approach to quickly accommodate small and frequent memory requests, and the other free lists devised with adaptation upon a typical binary buddy mechanism for bigger requests in harmony for the .improved performance. Comprehensive simulation results show that the proposed scheme outperforms QHF which is known to be effective in terms of memory fragmentation up to about 16%. Furthermore, the memory allocation failure ratio is significantly decreased and the worst case execution time is predictable.

• Journal of the Korea Society of Computer and Information
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• v.14 no.1
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• pp.35-45
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• 2009

According to the widespread use of ontology documents, a management system which store ontology data and process queries is needed for retrieving semantic information efficiently. In this paper I propose a storage schema that stores and retrieves semantic web documents based on RDF/RDFS ontology language developed by W3C in a relational databases. Specially, the proposed storage schema is designed to retrieve efficiently hierarchy information and to increase efficiency of query processing. Also, I describe a mechanism to transform RQL semantic queries to SQL relational queries and build up database using MS-ACCESS and implement in this paper. According to the result of implementation, we can blow that not only data query based on triple model but also query for schema and hierarchy information are transformed simply to SQL.