• Journal of Pharmaceutical Investigation
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• v.35 no.3
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• pp.137-142
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• 2005

A rapid, selective and sensitive reversed-phase HPLC method for the determination of glipizide in human serum was validated and applied to the pharmacokinetic study of glipizide. Glipizide and internal standard, tolbutamide, were extracted from human serum by liquid-liquid extraction with benzene and analyzed on a Nova Pak $C_{18}\;60{\AA}$ column with the mobile phase of acetonitrile-potassium dihydrogen phosphate (10 mM, pH 3.5) (4:6, v/v). Detection wavelength of 275 nm and flow rate of 0.7 ml/min were fixed for the study. The assay robustness for the changes of mobile phase pH, organic solvent content, and flow rate was confirmed by $3^3$ factorial design using a fixed glipizide concentration (500 ng/ ml) with respect to its peak area and retention time. And also, the ruggedness of this method was investigated at three different laboratories using same quality control (QC) samples. This method showed linear response over the concentration range of 10-1000 ng/ml with correlation coefficient greater than 0.999. The lower limit of quantitation using 0.5 ml of serum was 10.0 ng/ml, which was sensitive enough for pharmacokinetic studies. The overall accuracy of the quality control samples ranged from 82.6 to 105.0% for glipizide with overall precision (% C.V.) being 1.13-13.20%. The percent recovery for human serum was in the range of 85.2 93.5%. Stability studies showed that glipizide was stable during storage, or during the assay procedure in human serum. The peak area and retention time of glipizide were not significantly affected by the changes of mobile phase pH, organic solvent content, and flow rate under the conditions studied. This method showed good ruggedness (within 15% C.V.) and was successfully used for the analysis of glipizide in human serum samples for the pharmacokinetic studies at three different laboratories, demonstrating the suitability of the method.

• Magazine of the Korean Society of Agricultural Engineers
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• v.32 no.3
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• pp.87-101
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• 1990

The purpose of this study is to estimate the flood discharge and runoff volume at a stream by using geomorphologic parameters obtained from the topographic maps following the law of stream classification and ordering by Horton and Strahier. The present model is modified from Cheng' s model which derives the geomorphologic instantaneous unit hydrograph. The present model uses the results of Laplace transformation and convolution intergral of probability density function of the travel time at each state. The stream flow velocity parameters are determined as a function of the rainfall intensity, and the effective rainfall is calculated by the SCS method. The total direct runoff volume until the time to peak is estimated by assuming a triangular hydrograph. The model is used to estimate the time to peak, the flood discharge, and the direct runoff at Andong, Imha. Geomchon, and Sunsan basin in the Nakdong River system. The results of the model application are as follows : 1.For each basin, as the rainfall intensity doubles form 1 mm/h to 2 mm/h with the same rainfall duration of 1 hour, the hydrographs show that the runoff volume doubles while the duration of the base flow and the time to peak are the same. This aggrees with the theory of the unit hydrograph. 2.Comparisions of the model predicted and observed values show that small relative errors of 0.44-7.4% of the flood discharge, and 1 hour difference in time to peak except the Geomchon basin which shows 10.32% and 2 hours respectively. 3.When the rainfall intensity is small, the error of flood discharge estimated by using this model is relatively large. The reason of this might be because of introducing the flood velocity concept in the stream flow velocity. 4.Total direct runoff volume until the time to peak estimated by using this model has small relative error comparing with the observed data. 5.The sensitivity analysis of velocity parameters to flood discharge shows that the flood discharge is sensitive to the velocity coefficient while it is insensitive to the ratio of arrival time of moving portion to that of storage portion of a stream and to the ratio of arrival time of stream to that of overland flow.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.1
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• pp.194-201
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• 2017

This study was conducted to determine the valid time for accurate detection of complete blood cell count (CBC) in pig blood using an automatic blood corpuscle analyzer (Hemavet 950FS). Blood samples were collected from 34 pigs (Duroc) with a 60 kg (${\pm}3.5$) body weight. Ten specimens with CBC parameters in normal range and with no hemolysis were selected among 34 samples and used in this study. Regarding leukocytes parameters, white blood cell (WBC), neutrophil (NE), and lymphocyte (LY) counts showed a low daily variation (coefficient of variation, CV), whereas monocyte (MO), eosinophil (EO), and basophil (BA) CVs were significantly high (19.7, 56.9, and 53.3%, respectively). On the other hand, all parameters of erythrocytes and thrombocytes showed stable daily variation. All parameters of leukocytes and thrombocytes were significantly reduced as storage time passed (P<0.01 or 0.001), except for lymphocytes (P=0.535). However, no significant differences were observed in parameters of erythrocytes from blood up to 120 hours. From above results, we assert that Hemavet 950FS is useful in analyzing CBC, except for MO, EO, and BA. For accurate detection of leukocyte and thrombocyte parameters, analysis should be performed within 4 hours after blood collection when using Hemavet 950FS. On the other hand, parameters of erythrocytes could be stably detected for at least 120 hours after blood collection.

• Solar Energy
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• v.10 no.1
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• pp.5-13
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• 1990

In the present investigation, experiments and numerical analysis during melting process of a phase change material were performed to research heat transfer phenomena generated by means of conduction and natural convection in the vertical tube at inward melting. The phase change material used in the experiments is 99 percent pure n-Docosane paraffin($C_{22}H_{46}$). In the results, it is found that the velocity of phase change interface at the top of tube is faster than at the bottom of tube because of the effect of natural convection, and the distribution of velocity at the liquid region is little affected by the initial temperature of phase change material. The velocity of phase change interface is slower as the initial temperature of phase change material is lower, and the effect of natural convection is larger as the aspect ratio of tube is larger. In tendency of heat transfer phenomena, the experimental results were well corresponded with numerical results. But there were a little disagrements between the results of experiment and numerical analysis because of the assumption of the constant volumetric expansion coefficient in numerical analysis.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.36 no.5
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• pp.769-777
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• 2016

The objective of this study was to evaluate the stream depletion from groundwater pumping in deep aquifer using the Ward and Lough's analytical solution (2011) which considers a two-layer leaky aquifer system. The calculated results for each pumping from the 110 wells beside streams showed a wide range of non-dimensional stream depletion, that is the streamflow depletion rate divided by the groundwater pumping rate, from lower than 0.1 to more than 0.9 on average for 5 years depending on the specific properties of well location. From the comparison with Hunt's solution (1999) of a single layer aquifer, the Ward and Lough's solution showed about 50% lower than the Hunt's solution due to the difference of hydraulic properties between the first and second layers as well as the lagged effect of vertical leakance. It was also found that the groundwater pumping has a minor effect on the stream depletion if the stream depletion factor (SDF) of the 1st layer is higher than about 1,000 or the SDF of the 2nd layer is higher than about 100, or the vertical leakance is smaller than $10^{-5}s^{-1}$. Furthermore, in the present study, the variations of the stream depletion were assessed according to the magnitude of unmeasured hydraulic properties such as transmissivity and storage coefficient of the 1st layer, vertical hydraulic conductivity of the 2nd layer, the streambed hydraulic conductance.

• Journal of Korean Society of Forest Science
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• v.99 no.1
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• pp.62-67
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• 2010

This study was carried out to develop local allometric biomass regression equations and to estimate aboveground biomass of red pine (Pinus densiflora S. et Z.) stands among three regions (Hadong, Hamyang, Sancheong) from the western regions of Gyeongnam province. We selected three natural red pine stands with similar stand ages (about 40-year-old) from each region. The allometric regression equations were significant in all tree components (P<0.05) and the determination of coefficient ($R^2$) ranged 0.87 from 0.99. There was a significant difference (P<0.05) in the biomass of tree components among three regions. The biomass was 173.3 Mg/ha in Hadong, 131.0 Mg/ha in Sancheong, and 66.5 Mg/ha in Hamyang. The proportion of biomass was 70.4-77.1% in stemwood, 10.9-15.2% in branch, 8.9-10.4% in stembark, and 3.1-4.4% in needle. The results indicated that red pine stands in the western Gyeongnam regions showed the significant difference of aboveground biomass which was attributed to site quality and stand density.

• Korean Journal of Agricultural Science
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• v.17 no.2
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• pp.102-114
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• 1990

This study was carried out to analyze the operation of the sluice gates by taking Sabkyo Reservoir as the model, and to examine the formulae of the design criteria for the Agricultural Land Improvement Project by hydraulic model experiments. The results were summarized as follows ; 1. According to the records of gate operation for 9 years, the mean height of the opened gates was 4.13 m, the mean number of operated gates were 4.04, the average annual number of operation were 67 times, the average annual operating time were 192.5 hours, and the average operating time were 2.88 hours. 2. The water supplied through Sabkyo Reservoir was 88.15 megatons per year, which was about 1.4 times the effective storage capacity. And the annual volume of pumping in May, which is the most water demanding season, was 29.56 megatons in average. 3. As the submerged orifice was transformed into the surface orifice, the suggested formulae for the orifice flow on the design criteria for the Agricultural Land Improvement Project showed a discontinuous line on the transition zone. It should be improved, because it is different from the real hydraulic phenomena. 4. The formulae for the orifice flow which are divided into the submerged and surface orifices are being used. However, these formulae could be substituted for the formular, $q=C{\cdot}W\sqrt{2gH_1}$, if the discharge coefficient considering the reservoir water level, the sea water level, and the gate opening height is used.

• Analytical Science and Technology
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• v.9 no.4
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• pp.382-391
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• 1996

In this study, 12 different chemical species of fine ceramic($BaTiO_3$) were synthesized as the standard materials for the fast and accurate measurements of X-ray fluorescence spectrometry. Samples were diluted to sixteen times with the filling compound ($Li_2B_4O_7+LiBO_2$) in order to remove the matrix effect, and to get the convenient storage and homogeneity of ingredients. The matrix effects among the ingredients were corrected by the empirical coefficient method based on the Lucas-Tooth and Price model. The standard curve on 12 standard materials containing 15 elements were obtained by using X-ray fluorescence spectrometry at three different laboratories. The correlation factors of BaO, PbO, SrO, $Fe_2O_3$, $La_2O_3$, $SnO_2$, ZnO, $ZrO_2$, CaO indicated the relati vely good agreement over 0.995 among the three different laboratories. $SiO_2$ and $Al_2O_3$ showed the poor linearity because of their low fluorescence intensities.

• Korean Journal of Food Science and Technology
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• v.42 no.4
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• pp.390-397
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• 2010

Residual solvents in foods are defined as organic volatile chemicals used or produced in manufacturing of extracts or additives, or functional foods. The solvents are not completely eliminated by practical manufacturing techniques and they also may become contaminated by solvents from packing, transportation or storage in warehouses. Because residual solvents have no nutritional value but may be hazardous to human health, there is a need to remove them from the final products or reduce their amounts to below acceptable levels. The purpose of this study was to develop and evaluate an analytical method for the screening of residual solvents in health functional foods. Furthermore, the aim of this study was to constitute a reasonable management system based on the current state of the market and case studies of foreign countries. Eleven volatile solvents such as MeOH, EtOH, trichloroethylene and hexane were separated depending on their column properties, temp. and time using Gas Chromatography (GC). After determining the GC conditions, a sample preparation method using HSS (Head Space Sampling) was developed. From the results, a method for analyzing residual solvents in health functional foods was developed considering matrix effect and interference from the sample obtained from the solution of solvents-free health functional foods spiked with 11 standards solutions. Validation test using the developed GC/HSS/MS (Mass Spectrometry) method was followed by tests for precision, accuracy, recovery, linearity and adequate sensitivity. Finally, examination of 104 samples grouped in suits was performed by the developed HSS/GC/MS for screening the solvents. The 11 solvents were isolated from health functional foods based on vapor pressure difference, and followed by separation within 15 minutes in a single run. The limt of detection (LOD), limit of quantification (LOQ), recovery and coefficient of variation (C.V.) of these compounds determined by the HSS/GC/MS were found to be 0.1 pg/mL, 0.1-125 pg/g, 51.0-104.6%, and less than 15%, respectively. Using the developed HSS/GC/MS method, residual solvent from 16 out of 104 health functional products were detected as a EtOH. This method therefore seems t o be a valuable extension ofanalytical method for the identification of residual solvents in health functional food.

• Korean Journal of Food Science and Technology
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• v.45 no.3
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• pp.325-332
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• 2013

We aimed to determine the quality indicators for quality prediction of frozen green pumpkin in distribution. Freshly harvested green pumpkin slices were blanched under optimal conditions (confirmed in a preliminary experiment), quick-frozen at $-40^{\circ}C$ for 24 h, and stored at 0, -5, -15, and $-25^{\circ}C$. The correlation between each quality characteristic and the sensory preference rate was analyzed. There was a significant correlation between storage temperature-related drip rate and preference rate across all temperatures, except at$-25^{\circ}C$. Hunter values, a and b, showed relatively high correlation coefficient values at -5, -15, and $-25^{\circ}C$. Therefore, a change in the Hunter values should be considered when storing green pumpkin, because of their significant correlation with changes in the sensory preference and drip rates. Furthermore, they should be applied as quality indicators for the quality prediction of frozen green pumpkin in distribution.