• Food Science of Animal Resources
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• v.24 no.2
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• pp.151-155
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• 2004

This study was performed to develop the method of differentiation fresh and frozen meat by using the measurement of mitochondrial malate dehydrogenase. The principle of this experiment is based on the fact the enzyme proteins associated with mitochondria membrane could be released by freezing. The methods were studied by measurements of protein concentration of meat press juice, WHC (water-holding capacity), drip loss and mitochondrial malate dehydrogenase enzyme activity. Samples were stored at 4$^{\circ}C$ and -18$^{\circ}C$ during storage period, respectively. Protein concentration of meat press juice was ranged from 8.5 mg/mL to 12.7 mg/mL and increased by freezing below at -18$^{\circ}C$(p<0.05). The WHC was not significantly different between fresh meat and frozen chicken meat (p＞0.05). The amount of drip loss of fresh and frozen chicken meat at 4$^{\circ}C$ and -18$^{\circ}C$ was not significantly different (p＞0.05). Mitochondrial malate dehydrogenase activity of frozen meat (-18$^{\circ}C$) was significantly higher (p＜0.05) than that of fresh meat. Also, enzyme activity of frozen meat was maintained at the same level after 3 minutes reaction. But fresh meat had not this reaction. From these results, it suggests that mitochondrial malate dehydrogenase can be used as a promising enzyme to differentiate between fresh and frozen meat.

• Korean journal of food and cookery science
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• v.23 no.4 s.100
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• pp.452-460
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• 2007

As a preliminary study for developing cooking techniques to improve cooked rice flavor, we surveyed consumer perceptions on purchasing rice and the consumption of cooked rice. The quantity of rice purchased by consumers was in the range of $1O{\sim}20$ kg. The purchase criterion for 50% of the subjects was the taste of the cooked rice, and half of the subjects bought their rice at a supermarket. A majority (90%) of the subjects consumed the rice within 1 to 4 months, storing it raw in the package (32.4%) or in a container at room temperature. The rice was cooked for 2 to 5 persons (65%), 1 to 2 times a day (70%) with a pressure cooker (76%). The rice and water volume were measured by eye. The cooked rice was eaten immediately (41.8%) or in 1 to 2 days (48.6%) by keeping it in an electric cooker (63.6%). The subjects perceived the rice cultivar (37.9%) as the major factor for the cooked rice flavor, as well as the region of origin (21.3%), and storage period (11.9%); the cultivars of Ilmi, Ilpoom and Chuchung were preferred. In conclusion, the consumers regard rice as a principal food; however, the methods to measure rice and water are not standardized. To improve the taste of cooked rice, consumers depend on cooking equipment and the rice cultivar.

• Journal of Sericultural and Entomological Science
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• v.51 no.1
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• pp.36-40
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• 2013

To promote the consumption of mulberry fruit, we manufactured mulberry fruit wine with 'simheung' by different processing methods and analyzed cyanidin-3-glucoside(C3G) stability and antioxidant capacity. In the processing of mulberry fruit alcoholic drink, 3 minutes blanching using microwave inhibited the C3G destruction by maintaining the antioxidant capacity and inactivation of enzymes related to pigment's stability. And freeze-dried mulberry fruit was the highest C3G pigment content and antioxidant capacity. Nevertheless, this is not recommended because the economic burden of the cost of freeze-drying. In conclusion, when processing mulberry fruit wine, the addition of citric acid and the enzyme's inactivation treatment were considered more effective than storage containers (transparent glass, brown glass bottles, aluminum foil, green glass, translucent glass bottles).

• Polymer(Korea)
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• v.34 no.3
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• pp.191-197
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• 2010

To improve the curing method of anisotropic conductive film (ACF) at low temperature, it was studied to replace the thermal latent curing agent of imidazole compounds by the curing agent of cationically initiating type. Thermo-mechanical properties such as glass transition temperature, storage modulus, and coefficient of thermal expansion were investigated for the analysis of curing behavior. The reliability of ACF were observed in thermal cycle and high temperature-high humidity test. ACF using cationic initiator showed faster curing, lower CTE, and higher $T_g$ than the case of using imidazole curing agent, which is important for the high temperature stability. Furthermore, ACF using cationic initiator maintained a stable contact resistance in reliability test, although it was cured at low temperature and fast rate. With these results, it was confirmed that the curing method of epoxy had great effect on thermo-mechanical properties and reliability of ACF.

• Journal of Navigation and Port Research
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• v.29 no.10 s.106
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• pp.853-858
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• 2005

Recently, various novel numerical models based on Navier-Stokes equation have been developed for calculating wave motions in the sea with coastal or ocean structures. Among those models, Volume Of Fluid (VOF) method might be the most popular one, and it has been used for numerical simulations of wave motions including complicated phenomena of wave breakings. VOF method, however, needs enormous computation time and large computational storage memories in general, thus it is practically difficult to use this method for calculations in the case of random waves because long and stable computation (e.g for more than 100 significant wave periods) is required to obtain statistically meaningful results. On the other hand if the wave motion is potential motion, Boundary Element Method (BEM), which is a much faster and more accurate method than VOF method, can be effectively used. The aim of this study is to develop a new efficient model applicable to calculations of wave motion and/or wave-structure interactions under random waves. To achieve this, a strictly combined BEM-VOF model has been developed by making the best use of both methods' merits; VOF method is used in a restricted fluid domain around a structure where complicated phenomena of wave breakings may exist, and BEM is used in the other domains far from the disturbance where the wave motion may be assumed to be potential. The verification of the model was performed with numerical results for Stokes' 5th order wave propagation and a random wave propagation.

• Journal of KIISE
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• v.42 no.3
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• pp.307-319
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• 2015

Current ontology studies use the Hadoop distributed storage framework to perform map-reduce algorithm-based reasoning for scalable ontologies. In this paper, however, we propose a novel approach for scalable Web Ontology Language (OWL) Horst Lite ontology reasoning, based on distributed cluster memories. Rule-based reasoning, which is frequently used for scalable ontologies, iteratively executes triple-format ontology rules, until the inferred data no longer exists. Therefore, when the scalable ontology reasoning is performed on computer hard drives, the ontology reasoner suffers from performance limitations. In order to overcome this drawback, we propose an approach that loads the ontologies into distributed cluster memories, using Spark (a memory-based distributed computing framework), which executes the ontology reasoning. In order to implement an appropriate OWL Horst Lite ontology reasoning system on Spark, our method divides the scalable ontologies into blocks, loads each block into the cluster nodes, and subsequently handles the data in the distributed memories. We used the Lehigh University Benchmark, which is used to evaluate ontology inference and search speed, to experimentally evaluate the methods suggested in this paper, which we applied to LUBM8000 (1.1 billion triples, 155 gigabytes). When compared with WebPIE, a representative mapreduce algorithm-based scalable ontology reasoner, the proposed approach showed a throughput improvement of 320% (62k/s) over WebPIE (19k/s).

• Herbal Formula Science
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• v.22 no.1
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• pp.151-165
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• 2014

Objectives : The aim of this study was to evaluate the efficacy of Aconiti Lateralis Preparata Radix (AP) and Acanthopanacis Cortex (AT) extracts in bone-derived adipocyte OP9 cell, osteoclast and osteoblast-like MG63 cells. Methods : MTT assay was used to evaluate the cytotoxicity of AP and AT extracts on OP9, osteoclast and MG63 cells. OP9 cells were treated with AP and AT, and the alterations in fat storage in the cells were determined by the Oil red O. To explain effects of RANKL-induced osteoclast differentiation in bone marrow macrophages, we performed the TRAP staining. The protein level of CAAAT/enhancer binding protein alpha ($C/EBP{\alpha}$) and peroxisome proliferator-activated receptor ${\gamma}$ ($PPAR{\gamma}$) as a adipocyte differentiation marker, and adiponectin was examined using western blot in differentiated OP9 cells. Effects of related genes were confirmed by luciferase assay using reporter assay. Results : AP and AT was not toxic on OP9 and MG63 cells, but AT was a little cytotoxic to osteoclast at the dose of $100{\mu}g/m{\ell}$. They could inhibit differentiation of OP9 cells and osteoclast with results of oil red O staining and TRAP staining. By western blot, AP and AT decreased the expression of $PPAR{\gamma}$ and $C/EBP{\alpha}$ which is the key transcription factor in adipogenesis and adiponectin secretion. AT also inhibited the BMP-4 activity in luciferase assay. AP also inhibited BMP-4 and Wnt3a activity, stimulated ER-${\beta}$ activity but inhibited androgen receptor activity. Conclusions : These results show AP and AT can be useful in osteoporosis and obesity via inhibition of osteoclast and adipocyte differentiation.

• The Journal of Internal Korean Medicine
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• v.28 no.4
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• pp.671-680
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• 2007

Objectives : The purpose of this study was to examine the effect of Haeyeol-tang on infectious disease of respiratory system. Methods : Haeyeol-tang was used by cold storage after dilution with 1, 10 and $100{\mu}g/ml$ of PBS, from dried material as extracted material with extraction with 50% ethanol. Statistical significance was examined through measuring TNF-$\alpha$, RT-PCR, DPPH, nitric oxide(NO), and MTT of Raw264.7 cells activated with LPS. Results : For the toxicity experiment of Haeyeol-tang, almost no toxicity was shown on cells in the concentration ranges of 1, 10 and $100{\mu}g/ml$. For the effect of NO production, Haeyeol-tang showed significant (p<0.001) decrease of NO production, according to its concentration. For the effect on the eliminating activity of DPPH radical, Haeyeol-tang showed increasing tendency of eliminating activity of DPPH radical, according to its concentration. For the effect on revelation of NOS2 enzyme, Haeyeol-tang showed inhibiting tendency against the revelation of NOS2, according to its concentration. For the effect on the TNF-$\alpha$ production, Haeyeol-tang showed significant (p<0.001) decrease of TNF-$\alpha$ production. Conclusions : This result means that Haeyeol-tang is effective for anti-inflammation, as well as excellent effect for anti-oxidation and anti-aging.

• Journal of Soil and Groundwater Environment
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• v.10 no.1
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• pp.6-12
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• 2005

Contamination of soils and groundwater by leakage of petroleum compounds from underground storage tanks (USTs) has become great environmental issues. Conventional methods such as soil vapor extraction (SVE) used for the remediation of unsaturated soils contaminated with volatile organic compounds might not be applied for the removal of semi-volatile organic compounds such as diesel fuels and PCBs, which have low volatility and high hydrophobicity. The objective of this study is to develop a hot air injection method to remove semi-volatile compounds. Additionally, operation parameters such as temperature, air flow rate, and water content are evaluated. Experimental results show that diesel ranged organics (DROs) are removed in the order of volatility of organic compounds. As expected, removal efficiency of organics is highly dependent on the temperature. It is considered that more than $90\%$ of organic contaminants whose carbon numbers range between 17 and 22 can be removed efficiently by the hot air injection-extraction method (modified SVE) over the $100^{\circ}C$. It is also found that increased air flow rate resulted in high removal rate of contaminants. However, air flow rate over 40 cc/min is not effective for the operation aspects, due to mass transfer limitation on the volatilization rate of the contaminants. The effect of the water content on the decane removal is minimal, but some components show large dependence on the removal efficiency with increasing water content.

• Radiation Oncology Journal
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• v.14 no.3
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• pp.247-253
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• 1996

Purpose : The dose calculation program for the Buckler type remote after-loading system was developed. This program also can be used to calculate dose for various sealed sources. Materials and Methods : We determined the source length and distribution by dividing the program disk to 72 points. The dose rate for the each program disk and source was calculated. The dose rate table for the xy coordinate was established. The dose rate for the interesting points of the patient were calculated by using this table, We also made isodose curve from this calculations. Results : The storage size for the dose rate table were increased. But the calculation of the dose rate for the patient were carried out rapidly. So we could get real time calculation. Conclusion : By using this program, we could calculate the dose rate for the various points of the patient quickly and accurately. This program will be useful for the treatment with various linear sources.