• Title/Summary/Keyword: Storage Conditions

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Fractionation and Electrophoretic Patterns of Rice Proteins (쌀단백질의 분획 및 전기영동)

  • Kim, Su-Il;Jo, Do-Hyun
    • Applied Biological Chemistry
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    • v.26 no.1
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    • pp.65-72
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    • 1983
  • The composition of four rice protein groups is greatly affected by the extraction conditions. The extraction amounts of albumins and glubulines primarily depended on the temperature rather than the method of extraction. The total amount of glutelins, the major components of rice storage proteins, could be extracted by a successive extraction processes, extraction with 0.5% SDS-0.1M borate buffer(pH 8.3) followed by extraction with 0.5% SDS-0.6% ${\beta}-mercaptoethanol-0.1M$ borate buffer(pH 8.3). The extracted amounts of glutelin with these solvents were 54.1 and 45% respectively. The further purification of SDS soluble glutelins was achieved by Sephadex G-150 gel column chromatography. The molecular weight of the components in four protein groups has been estimated by SDS-polyacrylamide gel electrophoresis with or without ${\beta}-mercaptoethanol.$ The comparison of albumins and globulins by starch gel electrophoresis at pH 3.1 permitted us to identify seven rice varieties. However, at pH 8.95, the specific bands for Japonica type rice varieties were observed.

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Cooking Conditions and Textural Changes of Cooked Rice Added with Black Rice (흑미 혼용밥의 취반조건과 텍스쳐의 변화)

  • Kim, Du-Woon;Eun, Jong-Bang;Rhee, Chong-Ouk
    • Korean Journal of Food Science and Technology
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    • v.30 no.3
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    • pp.562-568
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    • 1998
  • Rice (Dongjin-byeo) added with black rices (Chindo and Suwon-415) was cooked and the changes of texture and color of this cooked rice were investigated by texture analyser and color & color difference meter. The hydration time to come to the equilibrium condition was at least 11 hr in black rices. The optimum ratio of water to cooked rice added with black rice was 1.6 (ratio of water to rice) and the hardness of cooked rice added with 5% Chindo black rice was $5.66\;kg_f$. Regardless of ratio of water to rice, Hunter a value increased as the ratio of black rice addition to rice increased, while L value decreased. The color elution rate of Suwon 415 was 4 times greater than that of Chindo black rice. The pH of the steep water of Suwon 415 at $20^{\circ}C$ during 120 min decreased from pH 6.40 to pH 6.16 as the steeping time increased. The optimum heating time by microwave oven of cooked rice added with black rice was between $90{\sim}120\;sec$ to recover the original texture after cold storage treatment of 7 days.

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Characteristics of film-type crystal growth mechanism of CO2 hydrate (CO2 하이드레이트의 film형 결정성장 거동에 관한 연구)

  • Lee, Hyunju;Kim, Soomin;Lee, Ju-Dong;Kim, Yangdo
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.23 no.2
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    • pp.93-100
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    • 2013
  • Many researches have been carried out to reduce and/or to capture the major global warming gases. Especially, the hydrate formation mechanisms were intensively investigated for carbon dioxide sequestration and storage process applications. In this study, the characteristics of film-type crystal growth mechanism of carbon dioxide hydrate were comprehensively examined. Carbon dioxide hydrate crystal was formed in semi-batch type stir reactor at various pressure conditions while the temperature was fixed to be constant to reduce and minimize the guest gas solubility effects. A supply gas composition was 99.999 % of Carbon dioxide, the observation data was collected by optical microscope adopted CCD camera (Nikon DS-5M/Fi1/2M-U2). This study revealed that the guest gas pressure changes significantly altered the crystal growth mechanism and film growth rate of carbon dioxide hydrate crystal. The critical pressure of the carbon dioxide hydrate of crystal growth mechanism change was found to be 2.0 MPa. The capillary force and gas concentration gradient also significantly changed the film-type crystal growth mechanism of carbon dioxide hydrate crystal.

Research for Calibration and Correction of Multi-Spectral Aerial Photographing System(PKNU 3) (다중분광 항공촬영 시스템(PKNU 3) 검정 및 보정에 관한 연구)

  • Lee, Eun Kyung;Choi, Chul Uong
    • Journal of the Korean Association of Geographic Information Studies
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    • v.7 no.4
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    • pp.143-154
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    • 2004
  • The researchers, who seek geological and environmental information, depend on the remote sensing and aerial photographic datum from various commercial satellites and aircraft. However, the adverse weather conditions and the expensive equipment can restrict that the researcher can collect their data anywhere and any time. To allow for better flexibility, we have developed a compact, a multi-spectral automatic Aerial photographic system(PKNU 2). This system's Multi-spectral camera can catch the visible(RGB) and infrared(NIR) bands($3032{\times}2008$ pixels) image. Visible and infrared bands images were obtained from each camera respectively and produced Color-infrared composite images to be analyzed in the purpose of the environment monitor but that was not very good data. Moreover, it has a demerit that the stereoscopic overlap area is not satisfied with 60% due to the 12s storage time of each data, while it was possible that PKNU 2 system photographed photos of great capacity. Therefore, we have been developing the advanced PKNU 2(PKNU 3) that consists of color-infrared spectral camera can photograph the visible and near infrared bands data using one sensor at once, thermal infrared camera, two of 40 G computers to store images, and MPEG board to compress and transfer data to the computer at the real time and can attach and detach itself to a helicopter. Verification and calibration of each sensor(REDLAKE MS 4000, Raytheon IRPro) were conducted before we took the aerial photographs for obtaining more valuable data. Corrections for the spectral characteristics and radial lens distortions of sensor were carried out.

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A Study on the Thermal Crack Control of the In-Ground LNG Storage Tank as Super Massive Structures (지하식 LNG 저장탱크 구조물의 온도균열 제어에 관한 연구)

  • Kwon, Yeong-Ho
    • Journal of the Korea Concrete Institute
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    • v.23 no.6
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    • pp.773-780
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    • 2011
  • In this study, thermal stress analysis are carried out considering material properties, curing condition, ambient temperature, and casting date of the mass concrete placed in bottom slab and side wall of the in-ground type LNG tank as a super massive structure. Also, based on the numerical results, cracking possibility is predicted and counter measures to prevent the cracking are proposed. For the tasks, two optimum mix proportions were selected. From the results of the thermal stress analysis, the through crack index of 1.2 was satisfied for separately caste concrete lots except for the bottom slab caste in 2 separate sequences. For the double caste bottom slab, it is necessary introduce counter measures such as pre-cooling prior to the site construction. Also, another crack preventive measure is to lower the initial casting temperature by $25^{\circ}C$ or less to satisfy 1.2 through crack index criterion. In the $1^{st}$ and $2^{nd}$ caste bottom slab, the surface crack index was over 1.2. Therefore, the surface cracks can be controlled by implementing the curing conditions proposed in this study. Since the side wall's surface crack index was over 1.0, it is safe to assume that the counter preventive measures can control width and number of cracks.

Internet-of-Things Based Approach for Monitoring Pharmaceutical Cold Chain (사물인터넷을 이용한 의약품 콜드체인 관리 시스템)

  • Chandra, Abel Avitesh;Back, Jong Sang;Lee, Seong Ro
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.39C no.9
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    • pp.828-840
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    • 2014
  • There is a new evolution in technological advancement taking place called the Internet of Things (IoT). The IoT enables physical world objects in our surroundings to be connected to the Internet. For this idea to come to life, two architectures are required: the Sensing Entity in the environment which collects data and connects to the cloud and the Cloud Service that hosts the data. In particular, the combination of wireless sensor network for sensing and cloud computing for managing sensor data is becoming a popular intervention for the IoT era. The pharmaceutical cold chain requires controlled environmental conditions for the sensitive products in order for them to maintain their potency and fit for consumption. The monitoring of distribution process is the only assurance that a process has been successfully validated. The distribution process is so critical that anomaly at any point will result in the process being no longer valid. Taking the cold chain monitoring to IoT and using its benefits and power will result in better management and product handling in the cold chain. In this paper, Arduino based wireless sensor network for storage and logistics (land and sea) is presented and integrated with Xively cloud service to offer a real-time and innovative solution for pharmaceutical cold chain monitoring.

Assessment of Effects of Groundwater Pumping from Deep Aquifer on Streamflow Depletion (죽산천 주변 암반층 지하수 양수로 인한 하천수 감소 영향 분석)

  • Lee, Jeongwoo;Kim, Nam Won;Chung, Il Moon;Cha, Joon Ho
    • Journal of Korea Water Resources Association
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    • v.48 no.9
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    • pp.769-779
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    • 2015
  • The streamflow depletion due to groundwater pumping from deep aquifer near the Juksan stream has been simulated, in this study, by using the surface water and groundwater integrated model, SWAT-ODFLOW in order to analyze the relationship between the stream depletion and hydraulic properties of aquifer and streambed, and to spatially assess the streamflow depletion. The simulated results showed that the streamflow depletion rate divided by the pumping rate for each well location ranges from 10% to 90% with reflecting the various well-stream distance, transmissivity, storativity, and streambed hydraulic conductance. In particular, the streamflow depletion exceeds about 50% of pumping rate for conditions with transmissivity higher than $10m^2/day$ or storage coefficient lower than 0.1. The simulated results in the form of spatial maps indicated that the spatially averaged percent depletion of streamflow is about 53.6% for five years of pumping which is lower than that for shallow aquifer pumping by 12.9%. From the spatially distributed stream depletion, it was found that higher and more rapid stream depletion to pumping occurs near middle-downstream reach.

Comparison of enzyme activities of the native and N-terminal 6xHis-tagged Fe supreoxide dismutase from Streptomyces subrutilus P5 (Streptomyces subrutilus P5의 천연 Fe superoxide dismutase와 N-말단 6xHis-태그가 결합된 Fe superoxide dismutase의 활성비교)

  • Park, Joong-ho;Kim, Jae-heon
    • Korean Journal of Microbiology
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    • v.52 no.2
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    • pp.230-235
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    • 2016
  • This study was carried out to analyze the differences in enzyme activity and stability between the native Fe superoxide dismutase (FeSOD) and the 6xHis-tagged superoxide dismutase (6xHis-FeSOD) of Streptomyces subrutilus P5. The optimum pHs for both native FeSOD and 6xHis-FeSOD were 7, while the pH range of the activity was narrower for the 6xHis-FeSOD. The native FeSOD was stable at pH 4-9, but the 6xHis-FeSOD lost its stability at pH > 9. The temperatures of the optimum activities were same for both types of enzymes. However, the heat stability of the 6xHis-FeSOD was clearly decreased; even at $20^{\circ}C$ the enzyme lost the activity after 360 min. In contrast, the native FeSOD was stable after 720 min at below $40^{\circ}C$. $H_2O_2$ inhibition was occurred already at 0.5 mM for the 6xHis-tagged enzyme. Therefore, from the results that the 6xHis-FeSOD retained the enzyme activity at pH 6-7 and $20-40^{\circ}C$, it can be assumed that the protein structure became destabilized under different storage conditions and sensitive to the enzyme inhibitor.

Effects of Nitrogen Application on the Patterns of Amino Acids, Nitrogen Contents and Growth Response of Four Legume Plants under Saline Conditions (염분 환경하에서 4종 콩과식물의 생장, 아미노산 및 질소함량에 미치는 질소원의 영향)

  • 배정진;추연식;김진아;노광수;송종석;송승달
    • The Korean Journal of Ecology
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    • v.26 no.3
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    • pp.135-142
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    • 2003
  • Four legume plants showed better growth by the external nitrogen supply rather than the symbiotic nitrogen fixation only under salt additions. In case of Glycine max and Phaseolus angularis, total nitrogen contents decreased by high salinity level but their amino acid levels significantly increased with the increase of salt treatments and indicated high soluble-/insoluble-N ratios. Cassia tora and Albizzia julibrissin contained less amino acids than G. max and P. angularis but total N (esp. insoluble N fraction) increased with higher salt levels. Asparagine occurred as a main amino acid especially in G. max and P. angularis and can be seen as potential N-storage form in these plants. It might be play an important role for the osmoregulation mechanism under the saline condition. Meanwhile, to investigate what kinds of nitrogen sources are effective for overcoming salt stress on soybean plants, various N forms and concentrations (NH₄NO₃-N, NO₃-N, NH₄NO₃-N; 2.5 and 5 mM) were additionally supplied to the salt gradient medium. Soybean plants treated with NH₄NO₃-N showed the best growth up to 40 mM NaCl and NO₃- fed plants indicated good growth even at 80 mM NaCl treatments. Contrary to NH₄NO₃- and NO₃- fed plants, NH₄/sup +/- fed plants showed remarkable growth reduction and died by 40 and 80 mM NaCl treatments after the first harvest (15th day). Consequently, these results suggest that salt excluding and resistant capacities of soybean plants under NaCl treatments are increased in order of NH₄ - N, control, NO₃- N and NH₄NO₃- N depending on N concentration except NH₄- N treatments.

Effect of Freezing Conditions on the Formation of Ice Crystals in Food during Freezing Process (식품의 동결중에 생성되는 빙결정에 미치는 동결조건의 영향)

  • 공재열;김정한;김민용;배승권
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.2
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    • pp.213-218
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    • 1992
  • The reaching time to the freezing point was to be fast in the order of 2% agar gel, 5% agar gel, 20% gelatin gel, pork, respectively. The freezing time and the passing time through the zone of the maximum ice crystal formation had linear relationship with the coolant temperature. The average diameter d$_{p}$ of ice crystal in a soybean protein gel and the moving of freezing front were represented an inverse proportion, and the moving velocity of freezing front was shown as 3.4$\times$10$^{-6}$ $\textrm{cm}^2$/sec from predicted theoretical formula. This value was very close to experimental results. The storage temperature did not give any influences for the growth of ice crystal in inside soybean protein gels during freezing conservation. The relationship between freezing condition and structure of freezing front was as follows : (moving velocity of freezing front) : (mass transfer rate of water at freezing point)$\times$(surface area of freezing front).

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