• Applied Microscopy
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• v.18 no.2
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• pp.102-118
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• 1988

This study was carried out to examine in vitro first whether the storage proteins, which the fat bodies of last larvae from Hyphantria cunea secrete into haemolymph, can be uptaked by the fat body cells of prepupa and then how the uptaked storage proteins can be accumulated in the fat body cells, if uptaken. The fat bodies which had been isolated from last instar larvae were cultured in 1 ml of Grace's insect medium containing $50{\mu}l$ of $^{3}H$-leucine (5.0 mCi/mol, Dupont) at $28{\pm}2^{\circ}C$ for 6 hrs. After the homogenates of the cultured fat bodies were centrifuged at 10,000 rpm for 10 minutes, the proteins included in the supernatant were separated by polyacrylamide gel electrophoreses (non-SDS, 6%). The next treatment of the electrophoresed gel was followed by rinsing. A storage protein band of several bands in the rinsed gel was sliced off. With elution of sliced storage protein bands in Tris-glycine buffer, the purification of radioactive storage proteins from fat bodies was finished. After the purified radioactive storage proteins were added in Grace's insect midis containing fat bodies of the prepupae, they were cultured for the randomly following minutes given as 3, 5, 7, 10, 15, 20 and 30 and for the randomly following hours given as 1, 2, 3 and 4 respectively. The double fixations of the cultured fat bodies in aldehyde and $OsO_4$, were followed by preparation of ultrathin sections from Epon-Araldite blocks through dehydration and embedding. The electron microscope autoradiographic treatment of all prepared sections were performed by the dipping method (Kim et al., 1987). The finally prepared specimens were examined with electron microscope. The fat body cells of the prepupa could be found to uptake the storage preteins of the last instar larvae, which were included in the culture medium, mostly by formation of coated vesicles. The in vitro uptake of the storage proteins actively occurred by 30 minutes after the addition of purified storage proteins in the culture medium. After culture for 7 minutes with the storage proteins, the uptaked radioactive storage proteins labelled a number of lysosomal granules. After culture for 20 minutes with the storage proteins, the radioactive storage proteins were finally incorporated and accumulated in lipid droplets and protein granules. The frequency in the fat body cell of radiolabelled lipid droplets occurs approximately 60%, while the frequency, in which the radiolabelled protein granules occurs in a fat body cell, is approximately 40%.

• Microbiology and Biotechnology Letters
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• v.9 no.1
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• pp.7-19
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• 1981

The production of gluconic acid from glucose by the resting cell system of Aspergillus niger was studied. It was found that the conversion products from glucose by the resting cell system were markedly influenced by the pH, temperature, substrate concentration, aeration, metal ions, cultivation time and storage conditions of the resting cells. Conversion products were identified as gluconic acid by the thin layer chromatography and infrared spectrophotometry. These conversions were greatly stimulated by addition of $Mg^{++}$, and S $n^{++}$, but showed inhibitory effects by C $u^{++}$, H $g^{++}$, C $d^{++}$, A $g^{+}$ and cyanide. For the optimum cell storage, it was effective to be kept at -$25^{\circ}C$ in 0.05M phosphate buffer solution of pH 7.0. The gluconic acid production by the resting cell system was more effective than those of the fermentation with respect to cultivation time, yield, recovery and re-use of the cell.l.l.l.l.l.l.

• Journal of Korea Society of Industrial Information Systems
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• v.15 no.2
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• pp.31-39
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• 2010

VoD(Video-on-Demand) servers have to provide timely processing guarantees for continuous media and reduce the storage and bandwidth requirements for continuous media. The compression techniques make the bit rates of compressed video data significantly variable from frame to frame. A VoD system should be able to provide the client with interactive operations such as fast forward and fast rewind in addition to normal playback of movie. However, interactive operations require additional resources such as storage space, disk bandwidth, memory and network bandwidth. In a stored video application such as VoD system, it is possible that a priori disk access patterns can be used to reserve the system resources in advance. In addition, clients of VoD server spend most of their time in playback mode and the period of time spent in interactive mode is relatively small. In this paper, I present the new buffer management scheme that provides efficient support for interactive operations in a VoD server using variable bit rate continuous media. Simulation results show that our strategy achieves 34% increase of the number of accepted clients over the LRU strategy.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.46 no.7
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• pp.29-38
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• 2009

In this paper, a high performance pipelined computing design of parallel multiplier-temporal buffer-parallel accumulator is present for the convolution-based non-cascaded architecture aiming at the real time Discrete Wavelet Transform(DWT) processing. The convolved multiplication of DWT would be reduced upto 1/4 by utilizing the filter coefficients symmetry and the up/down sampling; and it could be dealt with 3-5 times faster computation by LUT-based DA multiplication of multiple filter coefficients parallelized for product terms with an image data. Further, the reutilization of computed product terms could be achieved by storing in the temporal buffer, which yields the saving of computation as well as dynamic power by 50%. The convolved product terms of image data and filter coefficients are realigned and stored in the temporal buffer for the accumulated addition. Then, the buffer management of parallel aligned storage is carried out for the high speed sequential retrieval of parallel accumulations. The convolved computation is pipelined with parallel multiplier-temporal buffer-parallel accumulation in which the parallelization of temporal buffer and accumulator is optimize, with respect to the performance of parallel DA multiplier, to improve the pipelining performance. The proposed architecture is back-end designed with 0.18um library, which verifies the 30fps throughput of SVGA(800$\times$600) images at 90MHz.

• Journal of the Korea Society of Computer and Information
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• v.14 no.3
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• pp.41-49
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• 2009

Flash memory has become increasingly requestion for the importance and the demand as a storage due to its low power consumption, cheap prices and large capacity medium. This research is to design a high performance flash memory structure for the substitution of a hard-disk by dynamic prefetching of aggressive spatial locality from the spatial smart buffer system. The proposed buffer system in a NAND flash memory consists of three parts, i.e., a fully associative victim buffer for temporal locality, a fully associative spatial buffer for spatial locality, and a dynamic fetching unit. We proposed new dynamic prefetching algorithm for aggressive spatial locality. That is to use the flash memory instead of the hard disk, the proposed flash system can achieve better performance gain by overcoming many drawbacks of the flash memory by the new structure and the new algorithm. According to the simulation results, compared with the smart buffer system, the average miss ratio is reduced about 26% for Mediabench applications. The average memory access times are improved about 35% for Mediabench applications, over 30% for Spec2000 applications.

• Nuclear Engineering and Technology
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• v.52 no.2
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• pp.248-257
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• 2020

This study presents an initial design for a novel system consisting in a coupled nuclear reactor and a phase change material-based thermal energy storage (TES) component, which acts as a buffer and regulator of heat transfer between the primary and secondary loops. The goal of this concept is to enhance the capacity factor of nuclear power plants (NPPs) in the case of high integration of renewable energy sources into the electric grid. Hence, this system could support in elevating the economics of NPPs in current competitive markets, especially with subsidized solar and wind energy sources, and relatively low oil and gas prices. Furthermore, utilizing a prismatic-core advanced high temperature reactor (PAHTR) cooled by a molten salt with a high melting point, have the potential in increasing the system efficiency due to its high operating temperature, and providing the baseline requirements for coupling other process heat applications. The present research studies the neutronics and thermal hydraulics (TH) of the PAHTR as well as TH calculations for the TES which consists of 300 blocks with a total heat storage capacity of 150 MWd. SERPENT Monte Carlo and MCNP5 codes carried out the neutronics analysis of the PAHTR which is sized to have a 5-year refueling cycle and rated power of 300 MW_th. The PAHTR has 10 metric tons of heavy metal with 19.75 wt% enriched UO₂ TRISO fuel, a hot clean excess reactivity and shutdown margin of $33.70 and -$115.68; respectively, negative temperature feedback coefficients, and an axial flux peaking factor of 1.68. Star-CCM + code predicted the correct convective heat transfer coefficient variations for both the reactor and the storage. TH analysis results show that the flow in the primary loop (in the reactor and TES) remains in the developing mixed convection regime while it reaches a fully developed flow in the secondary loop.

• Journal of Korea Society of Industrial Information Systems
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• v.12 no.4
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• pp.32-40
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• 2007

A mobile device accesses large-sized data in object-based IP storage as an object unit over IP network. It relies heavily on a WLAN device, which has been known as one of the major power consumers. This paper designs and implements low-power object-based IP storage for mobile devices using an efficient WLAN power control. The proposed WLAN power control exploits prefetch buffer to maximize the idleness for incoming network traffic and controls available WLAN power modes to minimize the power consumption. Our experimental results reveal that the proposed WLAN control can save the total power consumption in a PXA270-based mobile device about 9% while playing the multimedia contents through an object-based IP storage device

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.203-206
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• 2000

Calcium carbonate $(CaCO_3)$ bead immobilized with alginate were developed as buffer system to enhance the cultivation efficiency of bifidobacteria. When Bifidobacteriuim longum KCTC 3128 and HLC 3742 were independently cultivated in 2.5-liter fermenter buffered the $CaCO_3$ bead, NaOH, $Na_2CO_3$, and $NH_4OH$. The proliferation of bifidobacteria and their storage stability were higher in culture broth buffered $CaCO_3$ beads than in culture broth buffered with NaOH, $Na_2CO_3$, and $NH_4OH$. Therefore, $CaCO_3$ bead may be useful as a buffer to enhance of the cultivation efficiency and viability of bifidobacteria.

• Journal of the Korean Institute of Gas
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• v.1 no.1
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• pp.73-80
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• 1997

This study was performed to develop the dispersion modeling methodology for quantitative prediction of the hazard distance or toxic buffer distance by comparing 10-min average, 30-min average, and 1-hr average maximum ground-level concentration with $Cl_2$ regultaion concentration, IDLH and ERPG-3 concentration for hazardous toxic gas, $Cl_2$ releases from the storage tank of the chemical plant facilities. For this dispersion modeling, the source term model, dispersion model, meteorological and topographical data are incorporated into the SuperChems model, and then the effects of the atmospheric stability, wind speed, and surface roughness length changes on the maxum ground-level concentration were estimated.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.17 no.4
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• pp.143-148
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• 2017

Flash Memory which is light and strong external shock as storage of small electronics like smartphone, digital camera, car black box has been widely used. Since the operation speed of the read operation and the write operation are different from each other, and the flash memory has the feature that it is not possible to overwrite, the delete operation is added to solve these problems. Wear-leveling must also be considered, since the number of erase times of the flash memory is limited. Many studies have been conducted on the substitutional algorithms of flash memory based on these characteristics of recent flash memories. So, to solve the problem that has existing buffer replacement algorithm this thesis divide page into 6 groups and when proposed algorithm select victim page, it consider reference page frequency and page recency.