• The Plant Pathology Journal
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• 제22권1호
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• pp.21-27
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• 2006

We immunized BALB/c mice with purified Cucumber green mottle mosaic virus isolate HY1 (CGMMV-HY1). Through the selection of positive clones that were grown on the HAT medium, four sensitive monoclonal clones (CG99-01, CG99-02, CG99-03, and CG99-04) were selected from 500 Hypoxanthine-guanine phosphoribosyltransferase positive hybridoma cells. Four sensitive clones of CGMMV-HYI were determined as IgM type of the subclass of mouse immunoglobulins Ig group. The titer of monoclonal antiserum against CGMMVHY1 was estimated 1:12,800 by the indirect ELISA. Although monoclonal antibodies (MAbs) from CG99-01 and from CG99-04 cross-reacted with Zucchini green mottle mosaic virus and Kyuri green mottle mosaic virus, MAb from the cell line CG99-03 was highly specific to CGMMV. No MAbs cross-reacted with Cucumber mosaic virus-Fny. Only CG99-04 reacted with Pepper mild mottle virus weakly and CG99-02 reacted with both CGMMV and KGMMV. CGMMV was detected from the rind of watermelon fruit by DAS-ELISA of CGMMV-HY1, but not from the flesh of watermelon. Average seed transmission rate of CGMMV in watermelon was $24\%$ from symptomatic watermelon collected from 5 regions of Gyeongnam province. CGMMV was detected by DAS-ELISA with specific MAb of CGMMVHY1 periodically from root stock, during the sequential process for nursery seedling in Haman. Necrotic spots on cotyledons of root stock seedling progressed to reveal the typical symptomatology on the primary leaves of scion upon grafting. Here, we have established MAb based ELISA system, which could accurately detect CGMMV from watermelon seeds, nursery seedlings, transplants and field samples from greenhouse or open out door field as well.

• 한국약용작물학회지
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• 제3권3호
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• pp.259-264
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• 1995

This study was carried out to find cultivation condition of peony in Euiseong district from 1994 to 1995. 1. Age distribution in cultivation of peony was from 30's to 50's and cultivated years was mainly from 5 years to 10 years, but there was more than 15 years 2. Cultivating area per house hold were 1300 pyeong average, there were moderate $400{\sim]1000$ pyeong and also those are occupied 22.6% more than 2000 pyeong. 3. The mothods of transplanting in Euiseong peony which Euiseong district was mostly divided peony but Yeongchen district used seedling stock. No. of sprout was mostly $3{\sim}4$ and planting time was mainly from Mid to Late of October. 4. The percentage of sterilization of soil and seedling stock was 77. 4%, in mulching by vinyl in tran­splanting 64. 5% in black vinyl and 25. 8% in white one. 5. Number of plant in peony planting was $3000{\sim}4000$ plant per l0a even more there was more than 8000 plant. 6. Chemical spraying time in peony field was 2 time in $1{\sim}2$ years cultivating, and $3{\sim}4$ time in $3{\sim}4$ years one. 7. Condition of fertilizer application in peony field was 40% in non-application, but $56.7{\sim}76.7%$ in 2 year to 3 years, and the time of fertilizer was mainly 3 time. 8. Drying time peony after havesting was from 12 to 24 hours by briquet stove and 24 hours by ma­chine of hot wind, also dry method of peony was mixed briquet stove and hot wind machine.

• The Plant Pathology Journal
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• 제21권4호
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• pp.369-376
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• 2005

Turnip mosaic virus (TuMV) is an infectious viral pathogen on the cruciferous crops, predominantly Chinese cabbage (Brassica campestris subsp. pekinensis) and radish (Raphanus sativus). On the basis of the symptom development in selective differential hosts from indicator host species, Chinese cabbage and Korean radish inbred lines, the representative eight isolates of TuMV were divided into two major groups/or six types. Group I includes Th 1, Ca-ad7, and Cj-ca2-1 isolates, while group II includes the other isolates (rg-pfl, r 9-10, Rhcql-2, Stock and Mustard). According to the molecular phylogenetic analysis, these isolates, however, divided into two groups and two independent isolates. Phylogenetic analysis indicated that four isolates (Tu 1, r9-10, Stock and Rh-cql-2) formed a distinct phylogenetic group, and the other two isolates (Ca-ad7 and Cj-ca2-1) also formed another group. Mustard and rg-pfl isolates did not seem to have any relationship with these two groups. Taken together, these results indicated that virulence differentiation on host plants, molecular phylogenetic analysis of the nucleotide and the deduced amino acid of TuMV coat proteins did not show any relationship. The multi-resistant lines, Wonyae 20026 and BP058 in Chinese cabbage represent valuable genetic materials that can be used for crucifer breeding programs on TuMV resistance, but not in Korean radish.

• 식물조직배양학회지
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• 제28권2호
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• pp.81-85
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• 2001

포도의 기내 삽목방법을 이용하였을 때 액아의 신장에 미치는 생장조절제의 영향과 이들 유묘의 virus 이병 정도에 관해 검정한 결과를 요약하면 다음과 같다. 3/4 MS 기본배지에 BA 0.1 또는 0.5 mg/L와 NAA 0.05 mg/L를 혼용한 것이 액아로부터 신초의 신장에 적합하였다. 기외이식용 상토는 vermiculite와 peat moss를 3: 1 등량용적 비율로 혼합한 경우 활착률이 가장 높았다. 포장에서 재배되고 있던 'Schuyler'외 2품종을 ELISA 검정한 결과 3품종 모두 GLRV Ⅰ, GFLV, ArMV에는 음성반응을 나타내었으나 GLRV Ⅲ에는 감염되 어 있었으며, 이병률은 30∼75%로 품종 간 차이가 현저하였다. 경정배양에서 얻은 유묘의 virus 검정 결과, GLRV Ⅰ, GFLV, ArMV는 3품종 모두 음성반응을 나타내었으나 'Schuyler'와 'Muscat of Alexandria'는 GLRV Ⅲ에 양성반응을 나타내어 이병률은 각각 37.5 %와 12.5%였다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.120-120
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• 2018

Intestinal microbiota is strongly connected to health of host. It has been reported that not only metabolic disease like diabetes and obesity, but psychological diseases are affected by composition of intestinal microbiota. To figure it out the importance of the composition and relationship between disease and microbiota, intensive researches have done with human and experimental animals. But, the composition of the intestinal microbiota could be affected by several factors such as experimental environments, feeding, water, and bedding. As a result, the data from each experimental group might be diverse. It also affects experiments about biological activities of plant materials. In this study, mouse intestinal bacteria were isolated from fresh feces and identified by 16S rRNA gene to use in biological activities of natural medicines. The fecal supernatant was anaerobically incubated at $37^{\circ}C$ for 48 hours. Colonies were picked up separately and incubated again in same condition to increase quantity to analyze and stock. The bacteria strains were listed up and could be used for many researches including biological activities of plant materials and change in composition of intestinal bacteria itself.

• The Plant Pathology Journal
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• 제18권5호
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• pp.284-287
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• 2002

The antifungal activity of super reductive water (SRW) against plant pathogenic fungi was examined to extend its application to integrated pest management (IPM) for plant diseases. Diluted solutions ($\times$1/10, $\times$1/25, and $\times$1/50) of SRW inhibited fungal growth of kiwifruit soft rot pathogen, Diaporthe actinidiae, in a concentration dependent manner, When kiwifruits were inoculated on wounds with mycelium blocks, stock and diluted solutions successfully inhibited the disease development. In addition to the high pH of the SRW, fungistatic activity was also considered as the cause of the antifungal effect against the pathogen. Whereas conidial germination of Magnaporthe grisea was not affected by the diluted SRW solutions, appressorium formation was significantly inhibited in a concentration dependent manner, With little harmfulness to human health and environment SRW could be used to control plant pathogenic fungi, particularly appressorium-forming fungal pathogens.

• Journal of Plant Biotechnology
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• 제5권2호
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• pp.101-105
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• 2003

To eradicate several viruses such as PVX, PVY, and PLRV which often cause considerable damages to the growth and yields of potatoes, several stems including shoot tips were excised from the potato plants grown for 50 days and electric shock was treated. Shoot tips excised from electric-shocked stems were transferred into the medium supplemented with antiviral compound, ribavirin to examine the combinatorial effect. When treated only with 20 mg/L ribavirin, PVX concentration in the regenerated plant-lets was slowly decreased as repeating sub-culture and finally, it took 32 weeks to reach completely PVX-free stock. With an electric shock treatment (10 mA electric current), all the replicates became free from PVY. However, PLRV was not completely eradicated from 94P70-4 and 93P29-3 lines even by treating with 10 mA electric shock. In this case, both electric shock and antiviral compound treatments in axillary buds from the stem segment were successful in eradicating viral contamination.

• 한국유기농업학회지
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• 제21권2호
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• pp.271-282
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• 2013

본 실험은 중부지방에서 벼 재배 시 유기 우분의 연용과 화학비료 시용이 유기 벼의 생산성, 볏짚의 사료가치 및 가축사육능력에 미치는 영향을 조사하였고, 결과를 요약하면 다음과 같다. 유기 벼의 생초수량, 건물수량, 식물 1개체 당 낱알의 건물 중 및 개수는 1, 2년차에는 화학비료 시용구가 유의하게 높았으나(p<0.05), 3년차에는 각 시험구 간 유의차는 없었다. 또한 수확지수는 3년 모두 화학비료 시용구가 유의하게 높았으며(p<0.05), 해가 거듭할수록 각 시험구 간의 차이가 약간 줄어드는 경향을 보였다. 또한 단위면적당 가축사육능력은 $K_{CP}$와 $K_{TDN}$의 평균값으로 비교해 보았을 때, 화학비료 시용구가 높은 값을 보였고, 각 시험구 간의 차이는 점점 줄어드는 경향을 보였다. 특히 $K_{ME}$의 경우 3년차에 우분 시용구가 유의한 차이는 없었지만 높은 값을 보이기도 하였다. 이상의 결과로 볼 때 중부 지방에서 유기 벼 재배 시 우분을 연용 했을 경우, 처음 1, 2년은 화학비료를 시용했을 때 보다는 수량이 낮았지만 3년차부터는 화학비료를 시용했을 때와의 격차가 줄어들기 시작하였으며, 추후 우분을 연용한다면 그 격차는 더욱 줄어들 것으로 판단된다.

• 한국환경생태학회지
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• 제22권4호
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• pp.396-408
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• 2008

국립산림과학원에서 발간된 임상도에 따르면 파주시 광탄면에 위치한 본 연구대상지는 활엽수 Ⅳ영급으로 분류되었으나 식생조사 결과 영급판정이 어려운 활엽수 천연림으로 구성되어 정밀 임상도 작성의 필요성이 제기되었다. 이에 정밀 현존식생 조사와 식생구조 및 연륜 분석 자료를 기초로 한 정밀 임상도 작성방안을 제안하고자 하였다. 정밀 현존식생유형 분석결과 22개 유형이었으며 자연림은 신갈나무림, 굴참나무림 등 11개 유형, 인공림은 밤나무림 등 6개 유형으로 구분되었다. 영급 판정을 위해 정밀 현존식생도를 바탕으로 방형구 42개와 89개 표본목의 목편을 채취하여 수령을 측정한 결과, 저지대에 입지한 인공림, 참나무류 소경목 지역은 II영급(29.8%), 나머지 지역은 III영급(57.6%)으로 토지이용이 가능하였고 Ⅳ영급 이상(8.8%)은 경계부의 급경사 능선부에 분포하여 토지이용은 불가능하였다. 이상과 같이 소규모 개발 예정지가 활엽수 자연림으로 판정된 경우 보전과 이용 판정에 있어 정밀 현존식생 조사, 식물군집구조 및 표본목 분석을 통한 정밀 임상도를 작성하여야 할 것이다. 정밀 임상도에는 식생의 자연성, 희소성, 다양성 등을 종합적으로 판단할 수 있는 자료가 포함되어 소규모 부지의 개발적정성 판단에 유용할 것으로 사료된다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2005년도 ICCAS
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• pp.1304-1308
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• 2005

This paper presents the case that the existing analog control systems were retrofitted with digital DCS(Distributed Control System)s for boiler unit in thermal power plant. Replacement of existing worn-out or obsolete analog control systems has been considered. Replacement of existing analog control systems with another analog control systems has lots of difficulties in maintaining the systems due to being out of stock. Due to those difficulties, existing analog control systems have been retrofitted with digital DCSs in many industrial sites. KEPRI(Korea Electric Power Research Institute) accomplished the project that retrofitted analog control systems with the developed DCSs for boiler unit in middle-scale coal-fired thermal power plant. The benefits of an upgrade to digital control include a increase of reliability due to system redundancy, ease of modifying the control logic and the parameters of function block, ease of maintenance due to available spare parts, improvement of information display, ease of modifying MMI(Man Machine Interface) displays, a increase in system availability, and improvement of control performance. This paper describes how to use the parameters of existing analog controllers, the implementation of digital PID controller, control system configuration for boiler unit in thermal power plant, some boiler control loops, control result during commissioning, and the comparison of boiler characteristic test data after retrofitting with the existing test data.