• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2002년도 춘계학술대회
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• pp.145-145
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• 2002

• 식물조직배양학회지
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• 제25권5호
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• pp.341-346
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• 1998

색소유전자의 전이를 통하여 새로운 색소발현체계를 가진 품종을 육종하기 위한 기초연구로 stock (Matthiola incana R. Br.)의 꽃봉오리로부터 cDNA-library를 합성하였고 screening을 통하여 anthocyanin 합성경로의 중요효소의 하나인 DFR (dihydroflavonol 4-reductase) 유전자를 분리하였다. 염기서열분석을 수행하여 분리유전자의 크기가 1450bp 이며 이중 coding region은 1029 bp 임을 확인하였다. 이미 밝혀진 다른 식물체의 DFR 유전자와 서로 염기서열의 일치성을 비교해 본 결과 외자엽식물인 옥수수와 보리와는 각각 61%를 보였으며, 쌍자엽식물인 페튜니아, 금어초, 거베라, 과꽃 그리고 카네이션 등 과는 66%-67%의 일치성을 나타내었다. 아울러 염기서열의 G/C 함량분석을 통하여 쌍자엽식물의 G/C 함량은 외자엽식물의 그것에 비해 매우 낮은 수치를 나타내었다. 분리유전자의 발현을 확인하기 위하여 인위적으로 기내에서의 전사와 해석을 수행한 결과 42-44 kd 크기의 단백질을 확인하였다. Southern blot 분석의 결과 DFR 유전자는 stock의 genome에 다른 대부분의 식물체와 유사하게 한 개가 존재하며 야생종과 돌연변이종의 stock을 분리 DFR 유전자를 probe 로 Northern blot 분석을 수행하여 돌연변이종인 lineK17b가 DFR 돌연변이임을 확인하였다.

• Plant Resources
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• 제7권1호
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• pp.1-6
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• 2004

In vitro proliferation system was achieved by using nodal segment excised from greenhouse grown juvenile stock plants of Alnus japonica. Stem explants were cultured on MS medium supplemented with different plant growth regulators of cytokinin and/or their combinations. The most effective cytokinin source was the combination of zeatin 2.0 mg/L and TDZ 0.05 mg/L producing the average number of shoots (16.8 $\pm$ 3.6). In addition, healthy roots were formed after small clumps of shoots were transferred to half strength of MS medium containing IBA 0.02 mg/L with optimal rooting capacity. Soil acclimatization was successfully conducted in cell tray containing artificially mixed soil with 92 % survival rate.

• 한국작물학회지
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• 제46권3호
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• pp.176-179
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• 2001

Source와 sink의 상대적 크기가 다른 고구마 품종간 이면접목을 통하여 생육에 미치는 고구마의 source와 sink의 상호관계를 분석함으로써, 고구마의 품종육성 및 재배법개선에 필요한 기초자료를 얻고자 수행한 결과를 요약하면 다음과 같다. 1 만장, 괴근수는 접목에 의하여 감소되었으며, 접목조합에 따라서 차이가 있었다. 2. 지상부의 생육은 접수, 지하부의 생육은 대목의 영향을 많이 받았다 3. 주만장과 괴근수에 대한 접목효과, 접수와 대목의 효과와 이들 간의 상호작용은 모두 유의성이 있었다. 4. 상저비율은 보통삽식＞자가접목＞이면접목 순으로 높았다.

• Journal of Plant Biotechnology
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• 제3권1호
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• pp.27-31
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• 2001

New types of cytoplasmic male sterility in Brassica species would be very useful for the production of F$_1$, hybrid seeds. Leaves and stems of rapid cycling stock of B.juncea (CrGC4-3) containing Anand CMS were used as experimental materials for plant regeneration from protoplast culture. Very high plant regeneration rate (85%) was found in the Kao & Michayluk medium supplemented with 2 mg/L zeatin, 0.5 mg/L BAP, and 1 mg/L NAA when only leaf, not stem, segments were cultured. Protoplasts were isolated from leaves using mixtures of enzymes (1% Cellulycin, 0.5% Macerozyme) in 0.4 M mannitol and 50 mM $CaCl_2$.$2H_2$O. Mcrocalli induced from protoplasts were transferred to the shoot regeneration medium containing 2 mg/L BAP, 2 mg/L zeatin, and 0.5 mg/L NAA. After 60 days of initial protoplast culture, regenerated plantlets were obtained, acclimatized, transplanted into the pots, and grown up to the flowering stage.

• Journal of Forest and Environmental Science
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• 제36권2호
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• pp.91-112
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• 2020

In Ethiopia, dry land vegetation including the fairly intact lowland and western escarpment woodlands occupy the largest vegetation resource of the country. These forests play a central role in environmental regulation and socio-economic assets, yet they received less scientific attention than the moist forests. This study evaluated the woody plant species composition, population structure and carbon sequestration potential of the A. senegal woodland across three distance gradients from the settlements. A total of 45 sample quadrants were laid along a systematically established nine parallel transect lines to collect vegetation and soil data across distance gradients from settlement. Mature tree dry biomass with DBH>2.5 cm was estimated using allometric equations. A total of 41 woody plant species that belong to 20 families were recorded and A. senegal was the dominant species with 56.4 IVI value. Woody plant species diversity, density and richness were significantly higher in the distant plots compared to the nearest plots to settlement (p<0.05). The cumulative DBH class distribution of all individuals had showed an interrupted inverted J-shape population pattern. There were 19 species without seedlings, 15 species without saplings and 14 species without both seedlings and saplings. A significant above ground carbon (5.3 to 12.7 ton ha^-1), root carbon (1.6 to 3.6 ton ha^-1), soil organic carbon (35.6 to 44.5 ton ha^-1), total carbon stock (42.5 to 60.7 ton ha^-1) and total carbon dioxide equivalent (157.7 to 222.8 ton ha^-1) was observed consistently with an increasing of distance from settlement (p<0.05). Distance from settlement had significant and positive correlation with species diversity and carbon stock at 0.64^⁎⁎ and 0.78^⁎⁎. Disturbance intensity may directly influence the variation of species composition, richness and density along the A. senegal woodland. The sustainability of the A. senegal woodland needs urgent protection, conservation and restoration.

• 식물병연구
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• 제13권2호
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• pp.98-103
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• 2007

포도나무의 추위피해는 여러 형태로 나타난다. 월동피해 중에는 기계적 피해, 동해 또는 수세의 약화 원인이 된다. 본 연구는 '거봉'의 뿌리혹병의 피해를 억제하기 위하여 저항성 대목의 선발과 월동법 개선을 통한 방제법을 개발하기 위하여 실시하였다. SO4, 5BB, 3306대목에 $10^4cfu/ml$의 뿌리혹병균(YK2823, YK3312, LMG259, HKA234)을 접종했을 때는 혹이 발병하지 않았으나 $10^6cfu/ml$의 높은 농도에서는 모든 대목에서 혹이 형성된다. 그러나 혹의 크기는 거봉에서보다 매우 작았다. 인공접종에 의하여 3품종의 뿌리혹병 저항성 대목을 선발하였다. 볏짚과 보온덮개로 줄기와 가지에 피복하는 월동법은 뿌리 혹병을 예방하는데 가장 효과적이었다. 이러한 처리방법은 포도나무가 월동하는 동안 수체의 온도 차이가 $9.6^{\circ}C$로 온도변화가 최소화되었고 동해발생이 없어 정상적으로 생육하였다.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2001년도 전력전자학술대회 논문집
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• pp.50-53
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• 2001

In this paper, we applied the Utility Interactive Photovoltaic-Wind Hybrid Generation System for stock raising waste disposal system. For the power consumption reduction of proposed system, we used the electromotive control system. So as to verify the propriety of proposed system, we made a Pilot Plant and examined the system.

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 2011년도 추계학술발표회 논문집
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• pp.39-41
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• 2011

The recycling rate of recovered paper in Korea is the highest in the world, 92%, but remanufacturing yield is low due to the extremely poor quality of the paper. The poor quality, in turn, influences to the reject amount in deinking process. To increase the yield of old newspaper recycling process, hydrophobic degree of inorganic pigments of deinking stock must be reduced. To determine the hydrophobicity, Pitch Potential Deposit Tester (PDT) was newly designed and applied with respect to the SB latex property of various quality used in Korea; its hydrophobic degree according to Tg, gel content, charge and particle size of latex and optimum designing condition of SB latex. And below are the conclusions: 1. The reason of excessive reject from old newspaper deinking process for total amount of printed ink is loss of inorganic pigments. When lipase, a biochemical catalyst, was applied with the purpose of preventing inorganic pigments loss about more than 70% of total reject weight and promoting hydration of pulp for deinking, deinking process yield of pre flotation secondary stage increased remarkably without any changes of deinking efficiency. 2. Lipase improved deinking stock by cutting ester linkage on surface of hydrophobic materials to promote its hydration. From this, it reached the conclusion that hydration degree of stock exercises significant effect on flotation deinking process yield. 3. Inorganic alkali promotes hydration of deinking stock. But there have been needs for more fundamental measures other than inorganic alkali of promoting hydration for yield improvement. For this, this study intended to find out reasons of chemical properties change on surface of hydrophobic material by change of pH. 4. Pitch Deposit Test (PDT) was performed for understanding principle of why surface of coating flake from OMG is hydrophobic and why it becomes hydrophilic when pH of stock is alkaline. As a result of this test, it is determined that swelling property by change of pH of latex film, which were used as coating adhesive is the reason for hydrophobic change. 5. Hydrophilicity of coating flake increased with hydrophilic pigments. And as more of SB Latex adhesive was used and higher of calcium hardness of stock became, its hydrophilicity decreased. SB Latex adhesive film is reformed by mechanical friction. For having hydrophilicity under neutral pH, strong bruising action such as kneading is required. 6. Because swelling of adhesive film decreases as Tg of SB latex gets lower and mean diameter gets smaller, it shows hydrophobicity under neutral pH. This lowers hydrophilicity of coating flake, which leads to easy elimination with flotation reject on DIP process. Therefore, for improving future flotation yield, it is necessary to develop to use eco-friendly clean SB latex by raising Tg and increasing mean diameter for recycling, and as a result, to reduce excessive loss of coating flake as a reject from deinking process.

• 생명과학회지
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• 제22권6호
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• pp.794-798
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• 2012

요네병은 Mycobacterium avium subspecies paratuberculosis에 의해 발생하며 반추동물에서 만성 쇠약을 일으켜 전세계적으로 양축산업에 커다란 경제적 손실을 일으키는 질병이다. 본 연구에서는 2008년부터 2009년까지 2년동안 의뢰된 총 3,927건의 국가 종축우의 혈청에 대한 요네병의 항체 양성률에 대해 조사한 결과 21두(0.5%)에서 항체 양성을 나타내었으며 이는 국내 일반 농가의 양성률(1.2-16.4%) 보다 현저히 낮은 수준이었다. 또한 연령별 양성률은 2세 미만에서 0.2% (3두), 2-3세에서 0.4% (2두), 3-4세에서 0.7% (3두), 4세 초과에서 0.9% (13두)로 전 연령에서 양성이 나타났으나 연령이 높아짐에 따라 양성률이 증가하는 경향을 보였다. 품종 별로는 젖소는 모두 음성이었으며 한우에서만 양성률 0.6%를 나타내었다.