• 대한수의학회지
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• 제26권2호
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• pp.245-249
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• 1986

The present study has been carried out to investigate the optimal condition on the blastogenesis of guinea pig lymphocytes. A microculture system in conjunction with a semiautomatic multiple sample harvester(SAMSH) was used to study the in vito optimal condition of guinea pig lymphocytes. Data were presented to show many variables that are Involved in studying the responses of guinea pig lymphocyte in a microculture system to the stimulation of Concanavalin A(Con A) and lipopolysaccharide (LPS). Analysis indicated that the conditions for optimal Con A as measured by incorporation of $^3H$-TdR include : (1) use of RPMI-1640 as culture medium, (2) use of $6{\mu}g$ of Con A, per culture, (3) use of $1{\times}10^6$ cells per culture. Conditions for optimal stimulation with LPS mitogen were similar to those used for Con A.

• 대한의용생체공학회:의공학회지
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• 제22권2호
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• pp.151-156
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• 2001

기능적 전기자극(FES)에 의한 사지운동의 효과적인 제어를 위해서는, 전기자극을 입력으로 하여 근력 및 운동을 정확히 출력하는 근골격모델이 요망된다. 이 연구에서는 FES에 의한 근력 및 운동을 보다 정확히 예측할 수 있는 모델을 작성하기 위하여, 기존의 근육모델에서는 포함되지 않았던 근력의 점진적 강화현상에 대한 기초적 성질을 조사하는 것을 목적으로 한다. 구체적으로는, 일정강도의 표면자극에 대한 근력의 강화현상이 주파수, 자극이력, 근육길이에 어떻게 의존하는지를 조사하였다. 실험결과로부터, 자극의 주파수가 높을수록 초기근력에 대한 자극중의 근력의 증가도는 작아지고 근력의 피크에 도달하는 시간이 짧아지는 것을 알 수 있었다. 선행 자극에 의해 근육의 내부적인 강화상태가 포화되면 근력은 추가적인 자극에 대해서도 더 이상 증가하지 않았다. 자극시의 근육의 길이는 근력강화에 큰 영향을 미쳤으며, 근육의 길이가 짧을수록 증가도가 컸다. 장래에는 이러한 결과를 토대로 한 새로운 근력강화의 모델이 요망된다.

• 대한수의학회지
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• 제38권1호
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• pp.183-199
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• 1998

The effects of electroacupuncture(EA) on gastrointestinal motility were investigated in 6 horses. Three acupuncture points ; Guan Yuan Shu(BL-26), Wei Shu(BL-21) and Da Chang Shu(BL-25) were stimulated for 20 minutes by EA at separate occasions under varying condition ; 2V-1Hz, 2V-5Hz, 2V-30Hz, 4V-1Hz, 4V-5Hz and 4V-30Hz. Myoelectric activity of stomach and cecum was monitored to investigate the gastrointestinal motility. Electromyogram(EMG) recordings were carried out before, 0, 20 minutes after and 40 minutes after the EA stimulation. EMG bipolar electrode was surgically implanted in seromuscular layer of greater curvature in the stomach and between medial band and ventral band in the cecum. The EA stimulation and monitoring were not commenced until 15 days after electrode implantation. The EA stimulation of Wei Shu influenced on stomach motility and that of Da Chang Shu on, cecum motility. However, the EA stimulation of Guan Yuan Shu influenced on both the stomach and the cecum motility. The myoelectrical spike burst amplitude of the stomach and the cecum was significantly(p<0.05) increased by 2V-1Hz stimulation, but the myoelectrical spike burst frequence of the stomach and the cecum was significantly decreased by 2V-30Hz or 4V-30Hz stimulation. The myoelectrical spike burst duration of the stomach and the cecum was significantly lengthened by 4V-30Hz and 2V or 4V-30Hz stimulation, respectively.

• JSTS:Journal of Semiconductor Technology and Science
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• 제17권1호
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• pp.35-41
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• 2017

We propose a novel multi-photodiode array (MPDA) based retinal implant IC with on/off stimulation strategy for a visual prosthesis with improved spatial resolution. An active pixel sensor combined with a comparator enables generation of biphasic current pulses when light intensity meets a threshold condition. The threshold is tuned by changing the discharging time of the active pixel sensor for various light intensity environments. A prototype of the 30-channel retinal implant IC was fabricated with a unit pixel area of $0.021mm^2$, and the stimulus level up to $354{\mu}A$ was measured with the threshold ranging from 400 lx to 13120 lx.

• 반도체디스플레이기술학회지
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• 제18권2호
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• pp.53-57
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• 2019

In this study, we prepared sputtering cathode for large sputtering thin film in the facing targets sputtering(FTS) system. Before fabrication of cathode equipment, we investigated optimal magnetic flux in the sputtering cathode by using magnetic field stimulation(Comsol). According to the result of magnetic field stimulation, we manufactured the cathode. After we mounted laboratory-designed cathode on FTS system, the discharge properties were observed in vacuum condition. In addition, ITO films were deposited on glass substrate and their electrical and optical properties were investigated by various measurements (four-point probe, UV-VIS spectrometer, field emission scanning electron microscopy(FE-SEM), Hall-effect measurement).

• 한국의학물리학회지:의학물리
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• 제30권1호
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• pp.22-31
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• 2019

Purpose: To develop a new diffusion-based functional MRI (fMRI) sequence to generate apparent diffusion coefficient (ADC) maps in single excitation and evaluate the contribution of b0 signal on neuronal changes. Materials and Methods: A diffusion-based fMRI sequence was designed with single measurement that can acquire images of three directions at a time, obtaining $b=0s/mm^2$ during the first baseline condition (b0_b), followed by 107 diffusion-weighted imaging (DWI) with $b=600s/mm^2$ during the baseline and visual stimulation conditions, and another $b=0s/mm^2$ during the last activation condition (b0_a). ADC was mapped in three different ways: 1) using b0_b (ADC_b) for all time points, 2) using b0_a (ADC_a) for all time points, and 3) using b0_b and b0_a (ADC_ba) for baseline and stimulation scans, respectively. The fMRI studies were conducted on the brains of 16 young healthy volunteers using visual stimulations in a 3T MRI system. In addition, the blood oxygen level dependent (BOLD) fMRI was also acquired to compare it with diffusion-based fMRI. A sample t-test was used to investigate the voxel-wise average between the subjects. Results: The BOLD data consisted of only activated voxels. However, ADC_ba data was observed in both deactivated and activated voxels. There were no statistically significant activated or deactivated voxels for DWI, ADC_b, and ADC_a. Conclusions: With the new sequence, neuronal activations can be mapped with visual stimulation as compared to the baseline condition in several areas in the brain. We showed that ADC should be mapped using both DWI and b0 images acquired with the same conditions.

• Korean Journal of Acupuncture
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• 제23권4호
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• pp.135-145
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• 2006

Objectives and Methods : Using the 2-channel DROS SQUID (Korea Research Institute of Standards of Science, 1999), the present study was carried out to record changes elicited in the auditory cortex by acupuncture stimulus on right TE5 (Waiguan) and GB43 (Xiaxi). Needle-retention stimulation of TE5 and GB43 were done for acquiring the brain activities changed by acupuncture. Acupoint TE5 and GB43 is known to be effective for the treatment of ear-related disease, such as deafness and tinnitus, and to be suspected to be related to the auditory cortex. Auditory evoked magnetic fields were recorded hem the left hemisphere of five subjects, in response to contralateral ear stimulation by irregularly spaced 170 msec long 1kHz tone busts (Korea Research Institute of Standards of Science) Results and Conclusions : The result as follows. The latency and amplitude of SQUID MEG responses at the human auditory cortex changed by needle-retention condition on TE5 were 4msec and 9.2 fT, respectively, which were slower and smaller than those of no-acupuncture condition. The latency and amplitude of SQUID MEG responses at the human auditory cortex changed by needle-retention condition on GB43 were 7.2 msec and 1.6 fT, respectively, which were slower and larger than those of no-acupuncture condition. The latency of SQUID MEG responses at the human auditory cortex changed by needle-retention condition on GB43 condition was slower than that of TE5 acupuncture condition.

• 디지털산업정보학회논문지
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• 제5권3호
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• pp.29-37
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• 2009

It is as result that study to apply mini magnet nerve curer in peripheral nervous system disease treatment. Simulator and embodied action power and Control Unit in cylinder form of magnet roof object firstly. Yielded service area about special quality of probe of loop object cylinder style and treatment area dimension and distance of treatment pulse secondly. Embody pulse forming course energy value by Probe's form by third, could embody treatment pulse by disease. Specially, through a special quality experiment, saved Damping pulse form and treatment digital forming etc. variously. Lately, embodied this to aid a little in disease treatment that follow that there is no invasion that there is no stimulation by medicine development. Neuralgia, muscular disease and Altzheimer, with stroke etc. is becoming the matter of concern and interest which disease of adult etc. it is same is important. And the melancholia in compliance with the modern direction of a ceremony which is complicated and garrulous, trillion it will cry and symptoms, it is a tendency where the emotional obstacle etc. nervous psychiatric disorder patient is increasing at class speed. But currently the applicator it will be able to treat like this disease almost it is a condition which is wholly lacking. Consequently like this disease it used magnetic stimulation and it diagnosed and the equipment it will be able to treat plan and it embodied.

• Journal of Pharmaceutical Investigation
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• 제38권5호
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• pp.295-302
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• 2008

In the present study, we examined the changes of uptake and efflux of taurine under various conditions inducing oxidative stress using rat conditionally immortalized syncytiotrophoblast cell line, TR-TBT cell, as blood-placental barrier in vitro model. In addition, we identified the characteristics of taurine transport in TR-TBT cells including general features, besides effect of calcium ion on taurine transport. Taurine uptake showed time, $Na^+$ and $Cl^-$ dependency, and was decreased by PKC activator in TR-TBT cells. Also, calcium free condition decreased taurine uptake and evoked taurine efflux in the cells. Oxidative stress induced the change of taurine transport in TR-TBT cells, but the changes were different depending on the types of stimulation inducing oxidative stress. The taurine uptake was increased by TNF-$\alpha$, LPS and DEM stimulation but decreased by $H_2O_2$ and NO stimulation. Also, the taurine efflux was regulated by TNF-$\alpha$ stimulation. In conclusion, the taurine transport through the blood-placental barrier was regulated in oxidative stress conditions, and these results demonstrated that oxidative stress affected the taurine supplies to fetus and taurine level of fetus.

• The Journal of Korean Physical Therapy
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• 제25권3호
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• pp.136-142
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• 2013

Purpose: The application of transcutaneous electrical nerve stimulation (TENS) is beneficial for joint movements, inhibition of spasticity, and the improvement of walking ability in patients with chronic hemiplegia. This study aimed to identify the effect of the application of TENS to the knee extensor on the affected side with respect to postural-sway distance and velocity during the sit-to stand movement. Methods: We included 19 patients with post-stroke hemiplegia in this study. They underwent measurements during the sit-to stand movement on a force plate with 5 different stimulation dosages applied over 7 s:No TENS, high-frequency and high intensity TENS, high-frequency and low intensity TENS, low-frequency and high intensity TENS, and low-frequency and low intensity TENS The 5 different condition were administered in random order. Results: The group that received TENS application exhibited a significant decrease in path length and average velocity of center of pressure (COP) displacement compared with the group that did not receive TENS application. TENS dosage at low frequency (3Hz) and high intensity yielded a significant decrease in path length, average velocity, mediolateral distance and anteroposterior distance of COP displacement (p<0.05). Conclusion: Our results demonstrated the effectiveness of the application of low-frequency TENS on STS performance. These findings provide useful information on the application of TENS for the reduction of postural sway during the sit-to-stand movement after stroke.