• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.76-76
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• 2003

Pluripotent embryonic stem cells can differentiate into beating cardiomyocytes with proper culture conditions and stimulants via embryo-like aggregates. We describe here the use of mouse embryonic stem (mES03) cells as a reproducible differentiation system for cardiomyocyte. mES03 cells growing in colonies were dissociated and allowed to re-aggregated in suspension [embryoid body (EB) formation〕. To induce cardiomyocytic differentiation, cells were exposed to 0.75% dimethyl sulfoxide (DMSO) during EB formation for 4 days and then another 4 days without DMSO (4+/4-). Thus treated EB was plated onto gelatin-coated dishes for differentiation. Spontaneously contracting colonies which appeared in approximately 4~5 days upon differentiation were mechanically dissected, enzymatically dispersed, plated onto coverslips, and then incubated for another 48~72 hrs. By RT-PCR, robust expression of cardiac myosin heavy chain $\alpha$, cardiac muscle heavy polypeptide 7 $\beta$($\beta$-MHC), cardiac transcription factor GATA4, and skeletal muscle-specific $\alpha$$_1$-subunit of the L-type calcium channel ($\alpha$$_1$CaC $h_{sm}$ ) were detected as early as 8 days after EB formation, but message of cardiac muscle-specific $\alpha$$_1$-subunit of the L-type calcium channel ($\alpha$$_1$CaCh) were reveled at a low level. In contrast, expression of myosin light chain (MLC-2V) and atrial natriuretic factor (ANF) were not detected during EB formation for 8 days. However, a strong expression of the atrial-specific ANF gene was expressed from day 8 onward, which were remained constant in EB. (cardiac specialization and terminal differentiation stage). Electrophysiological examination of spontaneously contracting cells showed ventricle-like action potential 17 days after the EB formation. This study indicates that mES03 cell-derived cardiomyocytes via 4+/4- protocol displayed biochemical and electrophysiological properties of subpopulation of cardiomyocytes.

• 디자인학연구
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• 제18권1호
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• pp.215-222
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• 2005

지식과 정보가 중심이 되고 있는 현재에 인터넷을 이용한 정보획득은 매우 중요한 일이라고 할 수 있다. 고령화가 진행이 되고 있는 우리나라에서 노인들이 보다 쉽고 편하게 인터넷을 이용할 수 있다면 정보를 공유, 이용하여 노년 이후에 새로운 사회 참여의 가능성과 함께 자아 개발, 여가 선용의 기회를 가질 수 있다. 본 연구에서는 현황조사와 문헌연구를 통하여 노인을 대상으로 실험을 실시할 프로토타입을 개발하여 노인들을 대상으로 실험을 하고 심층면접을 통하여 쇼핑페이지에 있어서 노인 계층을 위한 웹 인터페이스디자인의 가이드라인을 제시하였다. 노인 계층의 인터넷 사용 활성화를 위해서는 보다 커다란 시각적 요소의 사용과 단순화된 정보의 구조, 쉬운 마우스의 조작에 의한 네비게이션이 요구된다고 할 수 있다. 인터넷 사용인구의 증가와 함께 높은 연령층의 사용자도 증가하는 현 추세에서 노인들을 위한 웹 인터페이스디자인의 가이드라인이 적용이 된다면 노인 계층의 사용자는 보다 편의성이 강화된 웹사이트에서 쉽게 정보를 획득할 수 있을 것이다.

• 한국수산과학회지
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• 제26권2호
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• pp.198-203
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• 1993

춘계 마산만에서 적조현상을 일으키는 해산 와편모조류인 S. trochoidea의 cyst를 저니에서 채취, 분리, 발아시켰다. 환경요인에 대한 군증식속도의 실험 결과 수온 $20^{\circ}C$, 염분 $30\%0$, 조도 4,000lux에서 군증식 속도가 빨랐다. 그리고 증식 촉진 유기물질에 대한 실험으로서 규조류(Skeletorema costatum) 배양배지 여과액과 저질추출물질에 대한 실험 결과 각각 0.25ml/ml를 첨가하였을 때 각각 군증식속도가 빠르게 나타났다. 동종의 지방산을 분석한 결과 $C_{16:0}$이 가장 많고 $C_{18:0},\;C_{18:1},\;C_{22:0}$ 그리고 $C_{22:1}$ 등의 순으로 분리되었다.

• 한국의상디자인학회지
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• 제17권3호
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• pp.159-168
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• 2015

In this study, visual evaluation was wide pants with changes in length of pants and width of hem line of wide pants design to provide data which can enhance wearing image effects at the production of wide pants. According to the length of pants and width of hem line of wide pants, a total of 9 stimulants were chosen. Then, they were evaluated using a seven-point rating scale against 40 fashion students. The data has been analyzed by Factor Analysis, ANOVA, $Scheff{\acute{e}}$'s Test and the MCA method. The results of the study are as follows : 1. According to factor analysis, the components of visual evaluation depending on the length of pants and width of hem line of wide pants were divided into five factors: individuality, body correction, modesty, body length and cute. 2. According to visual evaluation depending on changes in the length of pants, no significant difference was found in all five positions. 3. According to visual evaluation depending on changes in the length of pants and width of hem line of wide pants, 'width of hem line 60, 100' revealed a significant difference in body correction. 'width of hem line 80' revealed a significant difference in body correction and body length. 4. In terms of interactions over changes in the length of pants and width of hem line of wide pants, no interaction effects were found in all five factors. According to multiple classification analysis(MCA) on the factors without interaction effects, length of pants had more effect on visual image in body correction, body length and cute. In other factors, more influence was observed depending on the width of hem line.

• 초등상담연구
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• 제5권1호
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• pp.205-226
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• 2006

The purpose of this study was to find out the relations between 6th graders' negative cognitive process and anger experience and aggressiveness. To achieve the goal, it conducted a test to examine children's negative cognitive process, anger experience, aggressiveness targeting 100 children of 6th grade in C elementary school, Gyeonggj province. Then it conducted SPSS 12.0 statistical program to get the results of correlation analysis and regression analysis. The outcomes were as follows. First, there was a meaningfully positive relation between a negative cognitive process and anger experience. In other words, children having more negative cognitive process seemed to experience the feelings of anger more often, this presented the important role of cognition while getting into a temper Second, it reported a positive relation between anger experience and aggressiveness. Children who have experienced anger more often showed more violent behaviors, especially there were more significant positive relations between trait-anger and aggressiveness compared to state-anger and aggressiveness. This could explain some possibilities that children with high level of trait-anger might outrage more often than others by recognizing the situations as anger stimulants. Third, when conducting a regression analysis, a negative cognitive process made an effect on anger experience which affected aggressiveness. However, it did not show a negative cognitive process making a direct effect on aggressiveness. This is considered that children could experience an anger while evaluating an event or object in a negative way based on individual belief, and emotional linguistic behavioral aggressiveness would be formalized as they express the sparked fury either internally or externally. In conclusion, this study proved that there were close relations between children's negative cognitive process and anger experience and aggressiveness. A negative cognitive process affects anger experience, and anger experience affects aggressiveness afterwards. A negative cognitive process affects aggressiveness through anger experience indirectly, and especially trait-anger among anger experience is the main factor to influence on aggressiveness. With consideration of these results, it is believed that mediation is important key to moderate the negative cognition and trait-anger in order to diminish children's aggressive behaviors. This study has a meaning to provide searching for manifold mediating methods between negative cognition and trait anger, with a fundamental resource.

• 한국의류산업학회지
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• 제8권5호
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• pp.496-504
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• 2006

This study was conducted for silver generation women, age 60 and more. Questionnaire was answered by the total 291 of women. Frequency analysis, t-test, ANOVA and Duncon-Test have been completed by using SPSS 12.0 tool. The conclusion of the study is below. First, silver generation women live in Seoul and South Gyeongsang Province. The data shows huge academic background gab among regions. Second, the following is the preferences found by 15 stimulants which expert groups identified based on demography. People in South Gyeongsang Province like default, tailored, three-button jacket more. People in South Gyeongsang Province prefer to Chanel jackets and people in their 70s prefer to it than in 60s, stand collar casual jacket for color and material, and the less they are educated, the more they like the jackets. And those who have less personal expenses tend to prefer to it. South Gyeongsang Province shows preference for semi polo-neck sweater. Highly educated did not show any preference for it. Women in their 70s tend to like blouses with round neckline. The data shows there is significant difference of preference for design, color and material for coloration vest between education levels. The less educated tends to like it. People in South Gyeongsang Province and those who live with their children are in favor with half sleeve jackets for colors and materials. All in Seoul and South Gyeongsang Province do not like three-quarter-length sleeve jackets because those jacket have wide and deep plunging neckline. The study showed people living in Seoul, in their 60s, highly-educated tend to favor polo shirts significantly. Seoul favor basic straight pants and people with any level of education excluding elementary prefer to it. The highly-educated and those who have a bigger allowance tend not to prefer to baggy trousers. In conclusion, Fifteen incentives (clothing design) for semi polo-neck sweaters, polo t-shirts, basic straight pants are more proper to silver generation women in their 60s, living in Seoul. Other designs are desirably applicable to customers on a national scale at middle prices.

• Journal of Yeungnam Medical Science
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• 제17권2호
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• pp.108-122
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• 2000

Background: Models of attention deficit hyperactivity disorder(ADHD) that have proposed a hypodopaminergic state resulting in hypofunction of the prefrontal circuitry have assumed a unitary dopamine system, which largely ignores the distinct functional differences between mesocortical dopamine system and nigrostriatal dopamine system. Purpose: The author's goal was to develop a pathophysiological model for ADHD with greater explanotory power than dopaminergic hypofunction hypothesis in prefronal circuitry. Material and Methods: Published clinical findings on ADHD were integrated with data from genetic, pharmacological, neuroimaging studies in human and animals. Results: Molecular genetic studies suggest that three genes may increase the susceptibility to ADHD. The three candidate genes associated with ADHD are each involved in dopaminergic function, and this consistent with the neurobiologic studies implicating catecholamines in the etiology of ADHD. Pharmacological data also provide compelling support for dopamine and noradrenergic hypothesis of ADHD. Neuroimaging studies lend substantial support for the hypothesis that right-sided abnormalities of prefrontal-basal ganglia circuit would be found in ADHD. Conclusions: The present hypothesis takes advantage of the major differences between the two pertinent dopamine systems. Mesocortical dopamine system, which largely lacks inhibitory autoreceptors, is ideally positioned to regulate cortical inputs, thus improving the signal-to-noise ratio for biologically valued signals. In this circuit, therapeutic doses of stimulants are hypothesized to increase postsynaptic dopamine effects and enhance executive functions. By contrast, symptoms of hyperactivity/impulsivity in ADHD are hypothesized to be associated with relative overactivity of nigrostriatal circuit. This nigrostriatal circuit is tightly regulated by inhibitory autoreceptoors as well as by long distance feedback from the cortex, and slow diffusion of therapeutic doses of stimulant via oral administration is hypothesized to produce a net inhibition of dopaminergic neurotransmission and improves hyperactivity.

• 대한한의학회지
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• 제38권2호
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• pp.53-60
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• 2017

Objectives: Beakdugu-tang (BDGT) consists of three medicinal herbs, and this prescription has long been used in treatment of various digestant problem in Korea. In this study, we designed to clarify mechanisms by which Korean traditional digestive medicine, BDGT, may exert anti-hepatic steatosis effects via improved insulin resistance cell model in human hepatocellular carcinoma (HepG2) and monocyte (THP-1). Materials and methods: The preparation of BDGT and constituents were extracted with 70% ethanol. HepG2 and THP-1 were treated with different concentrations of BDGT and constituents in the presence and absence of stimulants such as free fatty acids (FFAs) and oxidized low-density lipoprotein (ox-LDL), respectively. Results: The BDGT and its constituents inhibited the FFAs-stimulated lipid accumulation in HepG2 cells. Ethanol extracts of Amomum cardamomum (ACE) improved the ox-LDL induced insulin resistance in THP-1 cells. Also, treatment of monocytic cells with ACE increased anti-hepatic steatosis related gene levels including ABCA, ABCG and SR-B1. Conclusion: The results suggest that the ethanol extract of BDGT and its constituents potently inhibit the FFAs- and ox-LDL induced liver steatosis via improved insulin resistance.

• Journal of Korean Neurosurgical Society
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• 제37권2호
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• pp.129-136
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• 2005

Objective: Currently available therapies for human malignant gliomas have limited efficacy. Toxoplasma gondii, an obligate intracellular protozoan parasite, and Quil-A are nonspecific, potent immune stimulants. T. gondii is shown to have antitumor activity in some types of cancers. Therefore, this study is undertaken to evaluate the antitumor effect of Toxoplasma lysate antigen (TLA), alone or in combination with Quil-A, on human glioma U373MG and U87MG cells. Methods: The in vitro effects of TLA alone or in combination with Quil-A on the proliferation, invasion, and apoptosis of glioma cells were tested using MTT, Matrigel invasion, and DNA fragmentation assays, and the in vivo effects on the growth of gliomas were evaluated in athymic nude mice transplanted with glioma cells. Results: Treatment with TLA resulted in the suppressed proliferation and invasion of both U373MG and U87MG cells, in a dose-dependent manner. In addition, at high concentration, TLA induced glioma cell apoptosis. When TLA was administered in the mouse glioma model, malignant glioma growth was decreased. The combined treatment of TLA with Quil-A significantly inhibited the proliferation and invasion of cultured cells as well as tumor mass of implanted mice. Conclusion: TLA inhibits the proliferation and invasion of glioma cells in vitro and in vivo, and these antitumor effects of TLA are significantly enhanced by the addition of Quil-A.

• 한국실내디자인학회논문집
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• 제22권5호
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• pp.171-178
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• 2013

In this study, we sought to find out the effect of different emotional interior images of the community facilities in an apartment complex on a youth brain wave by analyzing an Electroencephalograph (EEG). Based on the frequency of usage, we selected learning facilities, cultural facilities, and sport facilities. For brain stimulation, the visual stimulants with three different emotional words, cheerful, gentle, and elegant, were used based on I.R.I image scale. Overall, total nine different emotional images were used. Based on our findings, we conclude that: first, in order to improve learning concentration of the youth, a learning facility for the youth needs to be designed by skillfully combining the soft and comfortable colors from the gentle image and the murky and dark colors from the elegant image. Second, when designing a cultural facility, it is preferable to consider the elegant image for a calm and comfortable space. Third, a sport facility design needs to preclude dark colors and apply light colors to create a dynamic and lively space. Furthermore, we found out that the youth has established static images of each functionally different facility through their experience and learning. Therefore, it is imperative to plan community facilities in an apartment complex in a way to connect the space function with the emotional characteristics of the youth in order to support and encourage energetic activities and learning of the community youth.