• Parasites, Hosts and Diseases
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• 제25권1호
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• pp.24-36
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• 1987

Praziquantel이 폐흡충에 미치는 영향을 광학 및 주사전자현미경으로 관찰하기 위하여 본 연구를 수행하였다. 가재에서 분리수집한 폐흡충의 피낭유충을 2마리의 개에 경구감염시키고 11주후에 도살하여 폐로부터 128마리의 성충을 회수하였다. Tyrode 용액에 prasiquantel을 0.01, 0.1, 1, 10, 1000mg/m1 농도로 희석하여 배양액으로 사용하였다. 배양액에 활발히 움직이는 충체를 8마리씩 넣고 $37^{\circ}C$에서 배양하면서 15분, 30분, 1시간, 6시간, 12시간, 26시간에 운동성 및 형태학적 변화를 해부현미경으로 관찰하였다. 또한 각 실험군의 충체를 광학 및 주사전자현미경 표본으로 제작하여 관찰하였다. Praziquantel에 작용된 충체는 즉시 수축하고 점차 이완되었으며 표피와 표피하층의 공포화, 소화관의 수축과 내강의 팍장, 소화관벽의 비후, 난소와 고환의 공포화 및 말단의 구형화, Mehlis선의 공 포화등이 관찰되었다. 주사전자현미경적 소견으로 소수포의 형성은 구흡반과 복흡반 사이의 표면에서 가장 먼저 관찰되었고 배양시간과 praziquantel의 농도가 증가합에 따라 전체표면으로 확산되었다. 고농도의 배양액에 배양한 충체의 측후방 표피에는 분화구모양의 손상이 관찰되었으며 점차 전체표피로 확산되었다. Praziquantel의 작용을 받은 폐흡충의 형태학적 변화는 표피뿐만 아니라 소화기관, 생식기관 기타 조직등 전부위에서도 일어나는 것이 관찰되었다. 특히 충체가 살아있는 농도에서도 생식기관이 파괴되어 산란에 지장을 초래할 것으로 예상된다. 이와같은 변화는 pragiquantel의 농도보다 배양시간에 보다 더 영향을 받는 것으로 보인다.

• Parasites, Hosts and Diseases
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• 제29권3호
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• pp.311-313
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• 1991

1990년 1월에 낙동강 하류의 선암천에서 채집한 참붕어에서 Metorchis orientalis의 피낭유충 감염상을 조사하였다. 조사한 참붕어 60마리에시 총 3,885개 (평균 65개)의 피낭유충이 검출되었으며, 어체 크기 별로는 80∼90mm 군에서 어체담 평균 143yf, 70∼79mm 군에서는 평균 54개, 60∼69mm 군에서는 평균 23개, 50∼59mm 군에서는 평균 40개의 피낭유충이 검출되었다 피낭유충은 타원형 또는 인형에 가까운 타원형이었으며 (평균 175.3×155.4㎛), 두꺼운 낭벽(평균 13.1㎛) 을 가지고 있었다. 이 연구 결과 낙동강 하류의 선암천에서 참붕어를 제 2 중간숙주로 하여 M. orientalis의 생활사가 잘 이루어지고 있음을 알 수 있었다.

• Parasites, Hosts and Diseases
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• 제57권6호
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• pp.647-656
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• 2019

Isthmiophora hortensis (Digenea: Echinostomatidae) is a dominant echinostome in animal reservoir hosts and humans in the Republic of Korea (Korea). We intended to investigate the infection status with this echinostome species in the several species of wild animals and describe the morphological characteristics in the faunistic view point. A total of 175 animal carcasses belonging to 3 families, i.e., Canidae, Felidae and Mustelidae, were collected from the southern regions of Korea from March 2010 to July 2017. Isthmiophora spp. worms were recovered from the small intestines of each animal under a stereomicroscope after washing of intestinal contents. Isthmiophora hortensis was recovered from 4 species of wild carnivores, i.e., Nyctereutes procyonoides (3/107: 2.8%), Mustela sibirica (11/31: 35.5%), Meles lucurus (2/3: 33.3%) and Martes flavigula (1/2: 50%). The other 3 carnivores comprising stray dogs, cat and leopard cat were negative for I. hortensis infection (0/2, 0/10 and 0/12, respectively). Specimens obtained from the Lutra lutra (6/8: 75%) were identified as a distinct species, I. inermis, by morphological comparison. Isthmiophora inermis has thinner body, elongate testes and different anterior limits of vitelline fields. Detailed morphological descriptions and comparisons with the morphological characteristics are provided. Conclusively, it was confirmed for the first time that 3 species of mustelid mammals, i.e., M. sibirica, M. lucurus, and M. flavigula, are to be the new definitive hosts of I. hortensis in Korea. Additionally, I. inermis is to be newly added in the Korean echinostome fauna.

• Restorative Dentistry and Endodontics
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• 제23권1호
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• pp.487-501
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• 1998

In the case of CAD/CAM ceramic inlay restorations, if isthmus width is widened too much, it may cause fracture of remaining tooth structure or loss of bonding at the luting interface because of excessive displacement of buccal or lingual cusps under occlusal loads. So to clarify the criterior of widening isthmus width, this study was designed to test the tensile bond strength and bond failure mode between dentin and ceramic cemented with luting composite resin cements. Cylindrical ceramic blocks(Vita Cerec Mark II, d=4mm) were bonded to buccal dentin of 40 freshly extracted third molars with 4 luting composite resin cements(group1 : Scotchbond Resin Cement/Scotchbond Multi-Purpose, group2 : Duolink Resin Cement/ All-Bond 2, group3: Bistite Resin Cement/Ceramics Primer, and group4:Superbond C&B). Tensile bond test was done under universal testing machine using bonding and measuring alignment blocks(${\phi}ilo$ & Urn, 1992). After immersion of fractured samples into 1 % methylene blue for 24 hours, failure mode was analysed under stereomicroscope and SEM. Results: The tensile bond strength of goup 1, 2 & 4 was $13.97{\pm}2.90$ MPa, $16.49{\pm}3.90$ MPa and $16.l7{\pm}4.32$ MPa, respectively. There was no statistical differences(p>0.05). But, group 3 showed significantly lower bond stregnth($5.98{\pm}1.l7$ MPa, p<0.05). In almost all samples, adhesive fractures between dentin and resin cements were observed. But, in group 1, 2 & 4, as bond strength increased, cohesive fracture within resin cement was observed simultaneously. And, in group 3, as bond strength decreased, cohesive fracture between hybrid layer and composite resin cement was also observed. Cohesive fracture within dentin and porcelain adhesive fracture were not observed. In conclusion, although adhesive cements were used in CAD/CAM -fabricated ceramic inlay restorations, the conservative priciples of cavity preparation must be obligated.

• Restorative Dentistry and Endodontics
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• 제23권1호
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• pp.236-246
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• 1998

The purpose of this study was to compare the degree of micro- leakage of new glass ionomer root canal sealer, Ketac-Endo(ESPE Co., Seefeld, Germany) with that of AH-26(De Trey Co., Ltd., U.S.A.). Root canal treatment using K -file, H -file, Gate Glidden drill was conducted on 49 extracted single-rooted teeth. 45 teeth were randomly divided into 3 experimental groups(15 teeth per group) and 4 teeth were used as the control group. Group 1 was used AH -26 sealer with the lateral condensation method for canal filling, group 2 was used Ketac-Endo with the single cone method and group 3 was used Ketac-Endo with the lateral condensation method. The control group was obturated with the single cone method without sealer. The teeth were covered with two coats of nail varnish after 48 hours of obturation. The teeth were immersed in India ink for 1 week and cleaned with methyl salicylate and then the degree of dye penetration were measured with stereomicroscope. The data were analyzed statistically by one-way ANOVA. The results were as follows: 1. 7 teeth in group 1, 5 in group 2, and 3 in group 3, were showed evidence of microleakage implying appropriate canal filling. 2, The mean average of microleakage was $0.17{\pm}0.32mm$ in group 1, $0.30{\pm}0.37mm$ in group 2. $0.10{\pm}0.21mm$ in group 3, showing that canal filling using the lateral condensation canal filling method with Ketac-Endo showed the least microleakage and using the single cone method with Ketac-Endo showed the largest amount of microleakage, 3. There were no statistically significant difference in the variation of microleakage among groups. From the results above, Ketac-Endo which has the advantage of glass ionomer, whether using the single cone method or the lateral condensation method, showed similar results as AH-26, but for clinical application it is thought that were studies on the properties of Ketac-Endo should be followed.

• Restorative Dentistry and Endodontics
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• 제25권2호
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• pp.202-211
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• 2000

The aim of this study was to compare the apical sealing ability of a new thermoplasticized gutta-percha filling technique, the Easy Filling and the Quick Obturation system with lateral condensation technique and Thermafil system to evaluate their clinical acceptabilities. Fifty-two extracted single-rooted teeth were instrumented to #35 using the .04 taper ProFile system. Four groups of 12 teeth were obturated by lateral condensation technique. Thermafil system and two new thermoplasticized gutta-percha techniques, the Easy Filling system and Quick Obturation system (Meta Dental co. Ltd. Korea), respectively. Four teeth served as controls. After the teeth were immersed in 2% methylene blue dye for 48 hours, they were resected horizontally at 1mm to 5mm level from the anatomical apex using a low-speed microtome. Each section was examined under a stereomicroscope at ${\times40}$ magnification and photographed. After each image was scanned, the leakage area was measured at each level using Brain 3 (Nosdia Tech., Korea) software. Leakage ratio was calculated for each group and was analyzed statistically to come up with the following results: 1. At 1mm level, the Quick Obturation system had the largest amount of apical leakage and it was statistically significant when compared with the lateral condensation group and the Thermafil group (p<0.05). 2. At 2mm and 3mm level, there were no significant difference of apical leakage among all four groups (p>0.05), and from 4mm level, no apical dye penetration was observed in all the groups. In conclusion, the apical seal produced by Easy Filling system and the Quick Obturation system was comparable to lateral condensation technique and Thermafil system except for the 1mm level. More improvement of the apical seal can be expected as the operator becomes skillful with the new techniques.

• Restorative Dentistry and Endodontics
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• 제23권1호
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• pp.346-356
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• 1998

The purpose of this study was to evaluate the sealing ability of various canal filling methods. Palatal roots of ninety extracted human maxillary molar teeth were resected at cementoenamel junction. Eighty of them were randomly assigned to four experimental groups, ten were served as positive and negative controls. All canals were prepared to # 40 using Profile. Experimental groups were obturated by lateral condensation technique, Thermafil technique, Continuous Wave of Condensation Technique, and down-pack & back-fill technique of Obtura-II, each with root canal sealer. Control groups were not obturated. Teeth were immersed in resorcinol-formaldehyde resin for 5 days at $4^{\circ}C$, and the resin was allowed to polymerize completely for 4 days at room temperature. Teeth were then ground horizontally at 1.5mm(level 1), 2.5mm(level 2), 3.5 mm(level 3) from the anatomical apex and examined with a stereomicroscope at ${\times}40$ magnification. The gap between the canal wall and the filling material, which was filled with the resin, was measured at each of the three levels. Each ratio of leakage was obtained by calculating the ratio of the area of the resin to the total area of the canal and was analyzed statistically (Rank-sum test). The results were as follows : 1. At the level 1, there was the greatest leakage in the Thermafil group and Obtura-II group, and the difference between the Obtura-II group and Continuous Wave of Condensation Technique group was statistically significant(p<0.05). 2. At the level 2, there was the least leakage in the Continuous Wave of Condensation Technique group, but there was no statistically significant difference between each group (p>0.05). 3. At the level 3, there was no statistically significant difference between each group(p>0.05).

• Restorative Dentistry and Endodontics
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• 제23권1호
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• pp.304-315
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• 1998

The purpose of this study was to evaluate the microleakage of light cured glass ionomer restorative materials in class 5 cavities. In this in vitro study, class 5 cavities were prepared on buccal and lingual surfaces of forty extracted human premolars and molars on cementum margin. These specimen were randomly divided into four groups of 10 each : Group 1 was Fugi II (control), Group 2 was Fugi II LC, Group 3 was Vitremer, and Group 4 was Dyract. Group 2 was also divided once more into 2 groups of 5 each : Group 2-1 was pretreated with dentin conditioner and Group 2-2 was not. All teeth were restored according to the manufacturer's instructions. After 500 thermocycling between $5^{\circ}C$ and $55^{\circ}C$, the 40 teeth were placed in 2 % Methylene blue dye for 24hr, then rinsed with tab water. The specimen were embedded in clear resin, then sectioned buccolingually through the center of restoration with a low speed diamond saw. The dye penetration on each of the specimen were then observed with a stereomicroscope at 20. The results of the study were statistically analyzed using the Student-Newman-Keuls Methods and the Mann-Whitney Rank Sum Test. Tooth restorative interfaces were evaluated using SEM analysis. Results were as follows, 1. Compared to conventional glass ionomer restoratioqs, all light cured glass ionomer restorations were fairly resistant to microleakage (P<0.05). 2. Groups 3 (Vitremer) and Group 4 (Dyract) were found to be the most resistant, Group 2 (Fugi II LC) fairly resistant, and Group 1 (Fugi II) least resistant to microleakage(P<0.05). 3. No significant differences were found between Group 2-1 and Group 2-2 (P>0.08). 4. With the backscattered SEM analysis, the degrees to which tight bonding occurred were also observed in all the groups except for Group 1. Group 4 showed the highest degree of tight bonding than any other materials used in this study.

• Restorative Dentistry and Endodontics
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• 제21권2호
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• pp.628-634
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• 1996

The purpose of this study was to investigate the effect of moisture on apical sealing properties of root canal. Fifty five single rooted human teeth were selected from maxillary and mandibular teeth. After removing crown portion at the cemento-enamel junction, all teeth were routinely prepared with step-back method. And then, the canals were dried with paper point and the teeth were randomly divided into 3 groups of 15 teeth each, and remaining 10 teeth were used as positive and negative control teeth : Group 1 were irrigated with 1ml of 95% alcohol and dried with air and paper point. Group 2 and 3 were intentionally contaminated with 0.05ml of 3.5% NaOCl or saliva, respectively. All the teeth were obturated with sealapex and gutta percha cone by lateral condensation technique, and covered with two coat of nail varnish after 48 hours of obturation. The teeth were immersed in india ink for 1 week and cleaned with methyl salicylate and then the degree of dye penetration were measured with stereomicroscope. The data were analyzed statistically by one-way ANOVA. The results were as follows : 1. All experimental groups showed varying degrees of dye penetration, and the mean degree of dye penetration was 0.1mm to 0.7mm. 2. Saliva contamination group(group 3) showed the highest amount of dye penetration, followed by NaOCl contamination group, then alcohol dried group, but there was no significant difference among three experimental groups. * This results suggest that there was no significant differences of apical leakage after canal obturation between alcohol dried canal and moisture present canals and the use of alcohol instead of paper point is unnecessary to dry the canals prior to canal filling. But other factors such as bacterial contamination and sealer discoloration by moisture must be considered in application of this results to clinical practice.

• Restorative Dentistry and Endodontics
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• 제21권2호
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• pp.517-529
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• 1996

The purpose of this study was to evaluate the apical sealing ability of the Thermafil endodontic obturation technique and to compare it with lateral condensation technique. 42 straight canals from extracted human anterior teeth and 42 curved canals(> $25^{\circ}$) from maxillary and mandibular molar teeth were selected. And 80 of them were divided into four groups, 20 canals respectively. The teeth in prior two groups had straight canals and the other two groups had curved canals. The rest of four canals served as positive and negative controls. After resecting anatomical crowns, all canals were prepared using a standard step-back technique. Lateral condensation was used to obturate two groups, one group of straight ones the other curved. And Thermafil obturators were also used in the same two groups. Obturated teeth were infiltrated by India ink for a week, decalcified and cleared with 5% nitric acid and methyl salicylate. The apical leakage and the frequency of filled lateral and accessory canals were measured with stereomicroscope and also apical extrusion of sealer and gutta-percha and obturation time were checked and the data were analyzed statistically(one-way ANOVA, t-test, Chi-square test). The results were as follows : 1. There was no significant difference in the degree of dye penetration between Thermafil and lateral condensation groups(p>0.05). 2. Apical extrusion of sealer and gutta-percha occurred significantly more often with Thermafil obturators in straight canals(p<0.05), but not significantly different in curved canals(p>0.05). 3. Canal obturation time with Thermafil obturators was significantly faster than lateral condensation (p<0.05). 4. The Thermafil groups showed a higher frequency of filled lateral and accessory canals than in the lateral condensation groups. But the difference was not statistically significant (p>0.05).