• Asian-Australasian Journal of Animal Sciences
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• 제24권7호
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• pp.957-964
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• 2011

The present study was conducted to evaluate the effects of fermented soybean meal (FSBM) on the level of cortisol hormone and immune-related serum proteins in weaned calves after experimentally induced lipopolysaccharide (LPS) challenge. Holstein neonatal calves (n = 21; 8 males and 13 females, BW = $42.2{\pm}6.15$ kg) were randomly allocated to one of two dietary treatments: SBM (control calf starter having soybean meal (SBM) as a main protein source) and FSBM (substitute SBM in control diet with FSBM) groups. All calves were fed milk replacer using an automatic milk-feeder according to step-down milking method and weaned at 7 weeks old. Experimental diets were given to calves ad libitum throughout the experimental period. For LPS challenge, all calves except negative control animals given phosphate buffered saline (PBS), were injected subcutaneously with Salmonella typhimurium LPS on day 7 (D7) after weaning (D0). No significant difference in growth performance and milk intake was observed between SBM and FSBM calves. Feeding FSBM diet resulted in significantly (p<0.05) higher LPS-specific IgG at D12 and D19 and LPS-specific IgA at D19 in peripheral blood. Calves fed with FSBM diet also had significantly (p<0.05) higher concentration of serum haptoglobin (Hp) at D8. Overall concentration of cortisol in FSBM group was considerably lower than that of SBM group. Results from current study indicate that FSBM may provide beneficial effects in alleviating weaning stress and enhance immune status of weaned calves.

• 한국초등수학교육학회지
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• 제15권2호
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• pp.317-332
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• 2011

이 연구에서는 각기둥의 정의 만들기 활동을 위한 학생용 활동지를 개발하여 초등학교 5학년 학생들에게 실행하고 그 결과를 분석하였다. 정의 만들기 수업에서 핵심은, 학습지에 차례로 제시되는 비(非) 예들이 포함되지 않도록 기존의 정의를 수정하여 새로운 정의를 만드는 것이었다. 연구 결과로, 학생들이 각기둥의 성질을 어떻게 인식하며, 비(非) 예를 제거하는 과정에서 각기둥의 정의에 사용되는 성질들로 무엇이 부각되는지를 알 수 있었다. 더불어 정의 만들기 활동을 통한 학생들의 수학적 정의에 대한 인식의 변화를 확인할 수 있었다.

• 한국식품과학회지
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• 제26권1호
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• pp.37-43
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• 1994

비압착 SPI두부의 제조조건의 결정을 위하여 수분첨가량, 2차 가열온도, 기름 및 덱스트린 첨가량에 따른 물리적 특성을 조사하였고, 각 조건에서 얻어진 견고성을 기준으로 다중회귀분석과 반응표면분석을 통하여 최적화하였다. 그 결과 덱스트린 첨가량 $10{\sim}15%$ 범위에서 두부의 견고성이 증가하였고, 2차 가열온도 $85^{\circ}C$, 수분은 8배 첨가하였을 때 최고의 견고성을 보였다. 종속변수를 견고성으로 하여 RSM 도시한 결과 덱스트린 첨가가 견고성에 미치는 영향은 가열온도의 영향보다 비교적 적었다. 기름첨가의 영향은 2차 가열온도 $85^{\circ}C$, 8배 수분, 25% 이상 기름 첨가구를 제외하고 제조 조건에 큰 영향을 받지 않았고 혼합첨가의 경우 25% 기름첨가구가 비교적 높은 값을 보였고, 가열시간 15분 이상인 경우 덱스트린 첨가량이 많을수록 견고성의 감소를 보였다. 비교적 견고하고 응집성있는 제조조건은 기름 25%, 덱스트린 $15{\sim}20%$ 첨가와 2차 가열온도 $90^{\circ}C$에서 $45{\sim}60$분이었다. 물리적 특성과 관능검사에 의해 결정된 비압착 SPI 두부제조를 위한 최적 조건은 균질화한 SPI-oil-dex-trin(100 : 25 : 15)에 SPI무게의 8배 수분을 첨가한 다음 $100^{\circ}C$에서 6분간 1차 가열을 하고 $40^{\circ}C$로 냉각시킨 용액에 혼합응고제(SPI : $CaSO_4$ : GDL=1g : 0.07g : 0.0075g)를 첨가하여 균질화한 다음 $90^{\circ}C$에서 45분간 2차 가열하여 상온으로 냉각시키는 것임을 알 수 있었다.

• Reproductive and Developmental Biology
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• 제34권3호
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• pp.143-151
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• 2010

Pluripotency of human embryonic stem cell (hESC) is one of the most valuable ability of hESCs for applying cell therapy field, but also showing side effect, for example teratoma formation. When transplant multipotent stem cell, such as mesnchymal stem cell (MSC) which retains similar differentiation ability, they do not form teratoma in vivo, but there exist limitation of cellular source supply. Accordingly, differentiation of hESC into MSC will be promising cellular source with strong points of both hESC and MSC line. In this study, we described the derivation of MSC like cell population from feeder free cultured hESC (hESC-MSC) using direct differentiation system. Cells population, hESC-MSC and bone marrow derived MSC (BM-MSC) retained similar characteristics in vitro, such as morphology, MSC specific marker expression and differentiation capacity. At the point of differentiation of both cell populations, differentiation rate was slower in hESC-MSC than BM-MSC. As these reason, to verify differentially expressed molecular condition of both cell population which bring out different differentiation rate, we compare the molecular condition of hESC-MSC and BM-MSC using 2-D proteomic analysis tool. In the proteomic analysis, we identified 49 differentially expressed proteins in hESC-MSC and BM-MSC, and they involved in different biological process such as positive regulation of molecular function, biological process, cellular metabolic process, nitrogen compound metabolic process, macromolecule metabolic process, metabolic process, molecular function, and positive regulation of molecular function and regulation of ubiquitin protein ligase activity during mitotic cell cycle, cellular response to stress, and RNA localization. As the related function of differentially expressed proteins, we sought to these proteins were key regulators which contribute to their differentiation rate, developmental process and cell proliferation. Our results suggest that the expressions of these proteins between the hESC-MSC and BM-MSC, could give to us further evidence for hESC differentiation into the mesenchymal stem cell is associated with a differentiation factor. As the initial step to understand fundamental difference of hESC-MSC and BM-MSC, we sought to investigate different protein expression profile. And the grafting of hESC differentiation into MSC and their comparative proteomic analysis will be positively contribute to cell therapy without cellular source limitation, also with exact background of their molecular condition.

• 한국경제지리학회지
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• 제14권2호
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• pp.116-128
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• 2011

서울 수도권 지하철 승객이 인터넷으로 구매한 채소 등의 신선 식품을 퇴근하는 도중에 이동 경로상의 지하철역 구내의 서비스 시설물에서 배달 받는다고 가정하면, 제기되는 문제는 어떤 지하철역에 서비스 시설물을 설치하면 얼마나 많은 승객들이 이용하는 가를 파악하는 것이다. 이러한 문제는 픽업 문제로 알려져 있고, 픽업 문제를 풀려면 교통 흐름을 설명하는 교통 네트워크가 있어야 되고 각 교통 흐름의 이동 경로도 알 수 있어야 된다. 서울 수도권 교통카드 시스템의 교통카드 트랜잭션 데이터베이스로부터 지하철 승객의 출발역에서 도착역까지 움직이는 이동 경로인 승객 흐름을 구할 수 있으므로, 서울 수도권 지하철 교통 시스템에서 픽업 문제는 승객 흐름을 최대한 많이 포착하는 서비스 시설물들을 설치할 지하철역들을 선정하는 것이다. 본 논문에서 서울 수도권 지하철 시스템과 지하철 승객들의 흐름을 픽업 문제의 모델에 맞추어 수식화하였고, 이를 기반으로 하여 지하철 승객들의 흐름을 기종점 통행 행렬을 만들어 단계별로 가장 많은 통과 승객수를 갖는 픽업 역을 계산해내는 빠른 휴리스틱 알고리즘을 제안하였다. 대용량 교통 네트워크와 교통 흐름에 해당되는 약 400여 개의 지하철역을 가진 서울 수도권 지하철 네트워크와 약 500만 명의 지하철 교통카드 트랜잭션들을 입력으로 하여 제안된 알고리즘으로 선정된 픽업 역들을 빠른 시간 내에 얻었고, 그 중에서 상위 10개 픽업 역들을 지하철 안내 지도에 표시하여 설명하였다. 그리고, 그 결과가 거의 최적해와 같음을 추가 실험을 통하여 검증하였다.

• 대한물리치료과학회지
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• 제8권2호
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• pp.977-987
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• 2001

The purpose of this study was to examine the effects of sensory integration therapy (SIT) on sensory' motor development and adaptive behavior of cerebral palsy children. The design of this study was quasi experiments with a non-equivalent pre- and post-test control design. Subjects of the study were arbitrarily chosen based on predetermined selection criteria among the cerebral palsy children who were treated as out-patients at two rehabilitation hospitals one in Seoul, and the other in Kyunggi-do. The study was conducted between early April and late July in 2000. Fifteen children were in the experimental group and eleven in the control group. The allocation was done based on ease of experimental treatment. A five-step SIT program was devised from a combination of SIT programs suggested by Ayres(1985) and Finks(1989), and an author-designed SIT program for cerebral palsy children. The experimental group was subjected to 20 to 30 minutes of SIT per session. two sessions a week for ten -week period. The effects of SIT were measured with respect to 9 sub-areas that can be administered to cerebral palsy children out of a total of 17 sub-areas in the Southern California Sensory Integration Test (SCSIT) developed by Ayres (1980). In addition. the scale developed by Russell (1993) for Gross Motor Function Measure (GMFM). and Perception Motor Development Test developed by 中司利一 et al.(1987) were also applied. Adaptive behavior was analyzed using guidelines in two unpublished documents - School-Age Checklist for Occupational Therapy by the Wakefield Occupational Therapy Associates, and the OTA-Watertown Clinical Assessment by the Watertown Occupational Therapy Associates-, and an author-developed Adaptive Behavior Checklist. Collected data were statistically analyzed by SPSS PC for chi square test, Mann-Whitney test, Wilcoxon signed rank test, and paired t-test. The results were as follows: 1. In sensory development, the experimental group exhibited a score increase compared to the control group, but the difference was not statistically significant, Although the experimental group showed improvements in all. 9 sub-areas compared to the control group, only right-left discrimination exhibited statistically significant change. 2. In gross motor development, the experimental group showed improvements in score compared to the control group, but it was not statistically significant. In fine motor development, the experimental group exhibited statistically significant improvements compared to the control group. In sub-area analysis, figure synthesis showed positive change. 3. In adaptive behavior development, post-experimental adaptive behavior scores were higher compared to pre-experimental scores with statistical significance. Furthermore, sub-areas emotional behavior, perception behavior, gross-fine motor function, oral-respiration function, motor behavior, motor planning, and adaptive response exhibited higher scores after SIT. In conclusion SIT was found to be partially effective in sensory and fine motor development, effective in all adaptive behavior areas, and not effective in gross motor development. Thus, this study has shown that SIT is an effective intervention for sensory development, fine motor development, and adaptive behavior for cerebral palsy children. But, for the effectiveness of SIT on gross motor development, further studies employing longer-time experiments are recommended.

• 한국산학기술학회논문지
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• 제19권10호
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• pp.330-338
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• 2018

비례-적분-미분(PID) 제어기는 단순한 구조와 넓은 범위의 운전영역에서 견고한 성능으로 인해 산업계에서 널리 사용되고 있다. 그러나 제어대상으로서 AVR(Automatic Voltage Regulator)은 전력 시스템의 파라미터의 변동에 강인하지 않다. 따라서 PID 제어기를 사용하여 AVR 시스템의 안정성과 성능을 향상시키는 것이 필요하다. 본 논문에서는 PSO(Partial Swarm Optimization) 알고리즘을 사용하여 AVR 시스템을 위한 최적 PID 제어기 파라미터를 결정하는 새로운 설계 방법을 제시한다. 제안하는 접근법은 쉬운 구현뿐만 아니라 안정된 수렴 특성 및 양호한 계산 효율과 우수한 특성을 갖는다. 또한, 제안 된 PSO-PID 제어기의 성능을 평가하기 위해 새로운 목적함수를 정의한다. 이 목적함수는 최대백분율 오버슈트와 정정시간이 설계사양으로 주어진 경우 이를 반영하기 위한 것이다. 이를 위해 ITAE 평가함수에 제약 조건을 위반하면 벌점을 부과하도록 하여 PSO 알고리즘이 PID 제어기 파라미터를 탐색할 때 설계사양을 만족하도록 하게 한다. 최종적으로 컴퓨터 시뮬레이션을 통해 제안한 PSO-PID 제어기는 단자전압 계단응답에 대해 주어진 설계사양을 만족할 뿐만 아니라 다른 유사한 최근의 연구보다 더 우수한 제어 성능을 보임을 확인하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권2호
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• pp.851-856
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• 2016

Background: Breast cancer is a major health problem among women around the world. Recent developments in screening and treatment have greatly improved the prognosis of patients with breast cancer in developed countries. However, in developing countries breast cancer mortality remains high.Breast cancer awareness is a first and important step in reducing breast cancer mortality. The development of a validated instrument to measure breast cancer awareness is crucial for the understanding and implementation of suitable health education programs to facilitate early deletion and minimize mortality. Objective: The objective of this study was to develop an instrument for the assessment of breast cancer awareness in Thai women. Materials and Methods: This methodological study was conducted in two stages: (1) literature searches and semi-structured interviews were conducted to generate items of the breast cancer awareness scale (B-CAS) which were subsequently examined for content and face validity, and (2) an exploration of the factor structure of the resulting instrument and an examination of its reliability. Data were collected using a self-administered questionnaire in Thai women aged 20-64 in August, 2015. Results: A total of 219 women (response rate 97.4 %) participated in this validation study. The B-CAS contains five domains with 53 items on breast cancer awareness: 1) knowledge of risk factors, 2) knowledge of signs and symptoms, 3) attitude to breast cancer prevention, 4) barriers of breast screening, and 5) health behavior related to breast cancer awareness. Items with a content validity index < 0.80 were excluded, and factor structure for the remaining items reflected the hypothesized five factor model. The scales based on all retained items was shown to have strongly internal consistency reliability (Cronbach's ${\alpha}=0.86$). Conclusions: The B-CAS provides good psychometric properties to assess breast cancer awareness in women. It can be used to examine breast cancer awareness in Thai women and it could lead to the development and evaluation of suitable educational interventions for raising breast cancer awareness. Future research should focus on further validating the B-CAS including an assessment of construct and criterion-based validity.

• Journal of Microbiology and Biotechnology
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• 제18권2호
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• pp.263-269
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• 2008

Hydrazinocurcumin (HC), a synthetic derivative of curcumin, has been reported to inhibit angiogenesis via unknown mechanisms. Understanding the molecular mechanisms of the drug's action is important for the development of improved compounds with better pharmacological properties. A genome-wide drug-induced haploinsufficiency screening of fission yeast gene deletion mutants has been applied to identify drug targets of HC. As a first step, the 50% inhibition concentration $(IC_{50})$ of HC was determined to be $2.2{\mu}M$. The initial screening of 4,158 mutants in 384-well plates using robotics was performed at concentrations of 2, 3, and $4{\mu}M$. A second screening was performed to detect sensitivity to HC on the plates. The first screening revealed 178 candidates, and the second screening resulted in 13 candidates, following the elimination of 165 false positives. Final filtering of the condition-dependent haploinsufficient genes gave eight target genes. Analysis of the specific targets of HC has shown that they are related to septum formation and the general transcription processes, which may be related to histone acetyltransferase. The target mutants showed 65% growth inhibition in response to HC compared with wild-type controls, as shown by liquid culture assay.

• Nutrition Research and Practice
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• 제14권5호
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• pp.438-452
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• 2020

BACKGROUND/OBJECTIVES: Brain senescence causes cognitive impairment and neurodegeneration. It has also been demonstrated that curcumin (Cur) and hesperetin (Hes), both antioxidant polyphenolic compounds, mediate anti-aging and neuroprotective effects. Therefore, the objective of this study was to investigate whether Cur, Hes, and/or their combination exert anti-aging effects in D-galactose (Dg)-induced aged neuronal cells and rats. MATERIALS/METHODS: SH-SY5Y cells differentiated in response to retinoic acid were treated with Cur (1 μM), Hes (1 μM), or a combination of both, followed by 300 mM Dg. Neuronal loss was subsequently evaluated by measuring average neurite length and analyzing expression of β-tubulin III, phosphorylated extracellular signal-regulated kinases, and neurofilament heavy polypeptide. Cellular senescence and related proteins, p16 and p21, were also investigated, including their regulation of antioxidant enzymes. In vivo, brain aging was induced by injecting 250 mg/kg body weight (b.w.) Dg. The effects of supplementing this model with 50 mg/kg b.w. Cur, 50 mg/kg b.w. Hes, or a combination of both for 3 months were subsequently evaluated. Brain aging was examined with a step-through passive avoidance test and apoptosis markers were analyzed in brain cortex tissues. RESULTS: Cur, Hes, and their combination improved neuron length and cellular senescence by decreasing the number of β-gal stained cells, down-regulated expression of p16 and p21, and up-regulated expression of antioxidant enzymes, including superoxide dismutase 1, glutathione peroxidase 1, and catalase. Administration of Cur, Hes, or their combination also tended to ameliorate cognitive impairment and suppress apoptosis in the cerebral cortex by down-regulating Bax and poly (ADP-ribose) polymerase expression and increasing Bcl-2 expression. CONCLUSIONS: Cur and Hes appear to attenuate Dg-induced brain aging via regulation of antioxidant enzymes and apoptosis. These results suggest that Cur and Hes may mediate neuroprotective effects in the aging process, and further study of these antioxidant polyphenolic compounds is warranted.