• 한국발생생물학회지:발생과생식
• /
• 제17권4호
• /
• pp.329-335
• /
• 2013

TCR subunits are members of membrane-bound receptors which allow the fast and efficient elimination of the specific fish pathogens have regulated function in adaptive immunity. Sequence structure of TCR subunits have been reported for various teleosts, but the information of each TCR subunit functional characterization through expression analysis in fish was unknown. In this study, we examined the gene expression of TCR subunits in the early developmental stages and observed transcript levels in various tissues from healthy adult olive flounder by RT-PCR. The mRNA expression of alpha subunit was already detected in the previous hatching step. But the transcripts of another TCR subunit were not observed during embryo development and increased after hatching and maintained until metamorphosis at the same level. It was found that all TCR subunits mRNAs are commonly expressed in the immune-related organ such as spleen, kidney and gill, also weak expressed in fin and eye. TCR alpha and beta subunit were expressed in brain, whereas gamma and delta were not expressed same tissue. The sequence alignment analysis shows that there are more than 80% sequence homology between TCR subunits. Because it has a high similarity of amino acid sequence to expect similar in function, but expression analysis show that will have may functional diversity due to different time and place of expression.

• 전자공학회논문지SC
• /
• 제40권3호
• /
• pp.208-214
• /
• 2003

기존의 아날로그 제어방식과는 달리 디지털 제어 방식은 기본적으로 마이크로프로세서를 포함하고 있기 때문에 아날로그 제어방식에서는 할 수 없었던 DC-DC 컨버터 내부 파라미터에 대한 모니터링이 가능하며, 아날로그 제어방식에서는 처음의 사양에 의해 고정된 출력전압을 얻었지만 디지털 제어 방식에서는 PC와 DC-DC 컨버터의 통신을 통하여 사용자가 원하는 임의의 전압을 얻어낼 수 있고 원격제어가 가능하다. 또 한 PC와의 통신을 통해 원거리에 있는 DC-DC 컨버터에 정확한 전압이 출력되고 있는지를 알 수 있는 원격 감시도 할 수 있다. 본 논문에서는 DSP(TMS320C31)을 이용한 DC-DC 컨버터를 설계하기 위해 간단한 Buck 컨버터를 구성하였으며, Buck 컨버터는 15V 입력으로부터 DSP의 에뮬레이터를 이용하여 PC상에서 원격으로 0V에서 5V까지 다양한 출력 전압을 만들 수 있다. PWM의 듀티(Duty) 제어를 위한 제어기로써는 DSP를 이용하여 간단하면서도 가장 많이 사용되는 PID제어기 중에서 PD제어기를 사용하였으며, 이 디지털 제어방식 컨버터의 실용성을 검토하기 위해 과도상태의 특성과 정상 상태의 특성을 비교, 분석하여 본 논문에서 설계된 DC-DC 컨버터의 우수성을 확인하였다.

• IMMUNE NETWORK
• /
• 제11권5호
• /
• pp.268-280
• /
• 2011

Background: Dengue virus, which belongs to the Flavivirus genus of the Flaviviridae family, causes fatal dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) with infection risk of 2.5 billion people worldwide. However, approved vaccines are still not available. Here, we explored the immune responses induced by alternating prime-boost vaccination using DNA vaccine, adenovirus, and vaccinia virus expressing E protein of dengue virus type 2 (DenV2). Methods: Following immunization with DNA vaccine (pDE), adenovirus (rAd-E), and/or vaccinia virus (VV-E) expressing E protein, E protein-specific IgG and its isotypes were determined by conventional ELISA. Intracellular CD154 and cytokine staining was used for enumerating CD4+ T cells specific for E protein. E protein-specific CD8+ T cell responses were evaluated by in vivo CTL killing activity and intracellular IFN-${\gamma}$ staining. Results: Among three constructs, VV-E induced the most potent IgG responses, Th1-type cytokine production by stimulated CD4+ T cells, and the CD8+ T cell response. Furthermore, when the three constructs were used for alternating prime-boost vaccination, the results revealed a different pattern of CD4+ and CD8+ T cell responses. i) Priming with VV-E induced higher E-specific IgG level but it was decreased rapidly. ii) Strong CD8+ T cell responses specific for E protein were induced when VV-E was used for the priming step, and such CD8+ T cell responses were significantly boosted with pDE. iii) Priming with rAd-E induced stronger CD4+ T cell responses which subsequently boosted with pDE to a greater extent than VV-E and rAd-E. Conclusion: These results indicate that priming with live viral vector vaccines could induce different patterns of E protein-specific CD4+ and CD8+ T cell responses which were significantly enhanced by booster vaccination with the DNA vaccine. Therefore, our observation will provide valuable information for the establishment of optimal prime-boost vaccination against DenV.

• 한국소음진동공학회논문집
• /
• 제26권2호
• /
• pp.157-164
• /
• 2016

이 논문은 MR 댐퍼를 적용한 맥퍼슨 타입의 반 능동 현가장치의 진동제어에 관한 연구를 보여준다. 맥퍼슨 스트럿의 기하학적 분석을 바탕으로 동역학 지배 방정식이 설립되었으며, 제어기 설계를 위해 평형 점 근처의 비선형적 운동이 선형화하였다. 이어서 시스템의 향상된 반응시간을 위해 새로운 적응 움직임 슬라이딩모드 제어기를 제안하였으며, 시뮬레이션을 통해 범프와 랜덤도로에서 차량으로 가해지는 진동에 따른 제어 성능을 기존슬라이딩모드 제어기 및 스카이훅 제어기와의 비교를 통해 우수성을 평가하였다.

• 정보보호학회논문지
• /
• 제27권1호
• /
• pp.147-162
• /
• 2017

IT 환경의 변화 속에서 다양한 서비스와 기기의 출현으로 인하여, IT 규모의 증가와 더불어 데이터의 복잡도가 높아지면서 많은 조직에서 보안 상황 인지를 위한 대량의 데이터 분석 및 처리에 어려움을 겪고 있다. 이에, 조직의 위험관리에 있어서 보안 상황의 인지와 대응이 늦어지는 문제의 해결을 위해, 시각화를 통한 보안 상황 인지 효과의 향상을 제안한다. 이를 위해, 본 연구에서는 시각화와 관련한 다양한 관점에서의 선행 연구를 통해 사용자 유형, 상황 인지 단계, 정보 시각화의 속성 등을 고려하여 효과적 시각화를 위한 평가 요인과 대안을 선정하고 AHP 계층 모델을 수립하였다. 이를 토대로, 다 기준 의사결정 문제의 해결을 위한 AHP 기법을 활용하여 효과적 시각화를 위한 요인과 요인별 대안의 중요도를 산정함으로써, 시각화의 목적 및 사용자 유형에 따라 보안 상황의 인지 효과를 향상할 수 있는 시각화 방안을 제시하고자 한다.

• Journal of Microbiology and Biotechnology
• /
• 제27권4호
• /
• pp.694-700
• /
• 2017

Clostridium difficile, which causes pseudomembranous colitis, releases toxin A and toxin B. These toxins are considered to be the main causative agents for the disease pathogenesis, and their expression is associated with a marked increase of apoptosis in mucosal epithelial cells. Colonic epithelial cells are believed to form a physical barrier between the lumen and the submucosa, and abnormally increased mucosal epithelial cell apoptosis is considered to be an initial step in gut inflammation responses. Therefore, one approach to treating pseudomembranous colitis would be to develop agents that block the mucosal epithelial cell apoptosis caused by toxin A, thus restoring barrier function and curing inflammatory responses in the gut. We recently isolated an antimicrobial peptide, Periplanetasin-2 (Peri-2, YPCKLNLKLGKVPFH) from the American cockroach, whose extracts have shown great potential for clinical use. Here, we assessed whether Peri-2 could inhibit the cell toxicity and inflammation caused by C. difficile toxin A. Indeed, in human colonocyte HT29 cells, Peri-2 inhibited the toxin A-induced decrease in cell proliferation and ameliorated the cell apoptosis induced by this toxin. Moreover, in the toxin A-induced mouse enteritis model, Peri-2 blocked the mucosal disruption and inflammatory response caused by toxin A. These results suggest that the American cockroach peptide Peri-2 could be a possible drug candidate for addressing the pseudomembranous colitis caused by C. difficile toxin A.

• 한국산업보건학회지
• /
• 제25권4호
• /
• pp.516-524
• /
• 2015

Objectives: This study aims to investigate the current status of knowledge, awareness and health risk concerns on occupational radiation exposures among firefighters in Korea. The results will provide basic information for developing a prevention program to minimize adverse health effects relating to radiation exposure among firefighters. Methods: A questionnaire was composed of general characteristics of participants, and their knowledge, awareness, health risk concerns relating to occupational radiation exposure. It was distributed by email to all of 307 firefighters in Jeollabukdo in April 2014 and 259 of them (response rate 84.3%) were responded. Answers were analyzed for descriptive statistics including frequencies and percentages. SPSS/WIN 18.0 program was utilized for statistical analysis of t-test, ANOVA and Pearson's correlation. Results: The average score of radiation protection knowledge was $5.83{\pm}1.77$ ($average{\pm}SD$) out of 10. The score of awareness and health risk concerns on radiation exposure were 4.27, 3.94 out of 5, respectively. The results indicated that the knowledge on the characteristics of radiation was marginal among the firefighters, while the awareness and health risk concerns relating to radiation exposures were relatively higher comparing to other professions. Conclusions: Knowing the characteristics of potential risks is the first step for minimizing the adverse health effects relating to the risks. Therefore, it is necessary to provide adequate training and information on radiation and exposure protection methods for firefighters.

• 해양환경안전학회지
• /
• 제15권4호
• /
• pp.393-399
• /
• 2009

해양사고의 대부분은 해상교통량이 많은 연안해역과 해수욕장, 방파제 등 많은 사람들이 찾는 연안지역에서 발생한다. 최근 해양레포츠의 활성화로 연안에서의 안전사고위험은 더욱 증가하고 있다. 그러나 연안의 안전사고에 대한 대책이 안전사고 증가속도에 적절하게 대응하지 못하고 육지와 바다를 연계한 안전관리가 체계적이지 못하다. 최근 5년 동안 발생했던 안전사고를 분석하여 사고발생을 최소화할 수 있는 안전관리 대책방안에 대하여 연구하였다. 연안의 안전사고는 육지와 바다가 접촉되는 연안지역에서 발생하기 때문에 종합적인 안전대책이 필요하며, 종합적인 안전망 구축을 위해서 제도상 법률상으로 주무기관이 일원화되어야 한다. 또한 사고발생시 신속하게 인명과 재산을 구조할 수 있는 사고대응 체제가 체계적이고 효율적으로 작동되도록 구축되어야 한다.

• 한국광학회지
• /
• 제8권4호
• /
• pp.327-332
• /
• 1997

2단계 메사 식각 공정과 유기 금속 화학 증착방법으로 높은 비저항을 갖는 Fe-도핑된 반절연 InP층의 전류 차단층을 갖는 10 Gb/s 광통신용 초고속 1.55.mu.m 궤환형 반도체 레이저 다이오드를 제작하였다. 제작된 DFB-LD의 특성은 발진 임계전류~15 mA, slope efficiency ~0.13 mW/mA, 동 저항 ~6.0.OMEGA.이었고, 발진 파장은 1.546 .mu.m이며, 6 Ith까지의 전류에도 인접 모우드 억압비, SMSR>40dB 이상 (CW상태)으로써 안정된 단일 모우드 동작을 보였다. DFB-LD의 소신호 주파수 특성으로 27 mA의 작은 구동전류에서 이미 -3dB 대역폭이 10 GHz에 도달하였음을 보여주었고, 또한 최대 -3dB 대역폭으로 구동전류 90 mA에서 ~18 GHz까지 얻는 우수한 소신호 주파수 특성을 보여주었다. 10 Gb/s DFB-LD 모듈 전송시험에 있어서, 1.55.mu.m 파장의 레이저 다이오드 모듈로 일반 단일모우드 광섬유와 분산천이 광섬유에 대해서 전송시험한 결과 에러평탄면(error floor)없이 각각 10 km, 80 km를 전송할 수 있었다.

• 대한지역사회영양학회지
• /
• 제21권1호
• /
• pp.37-52
• /
• 2016

Objectives: This study was performed to develop an assessment tool for middle aged women's health status based on dietary patterns, which will have practical applications in the working field of health and hygiene, aiming at improving the middle aged women's quality of life through their health improvement. Methods: As a first step, a literature review was conducted and the original data of '2008~2009 Korea Health and Nutrition Examination Survey' were reanalyzed. This analysis identified 65 preliminary questions that may be relevant to the study. After verifying the content validity by experts, the 65 questions were reduced into 51 questions. In order to secure higher validity of the candidate items, verification of their clinical validity was conducted among women aged between 45 and 60 years. Finally, an assessment tool was developed by applying weight and scoring. Results: Selected 51 questions were used to verify clinical validity and the results showed that 20 questions were relevant, nine questions ('regular meal time', 'regular amount of meal', 'intake frequency of dairy products', 'intake frequency of fruits', 'intake frequency of meat products', 'intake frequency of high cholesterol foods', 'intake frequency of salty foods', 'appetite', 'eat breakfast everyday') were related to dietary life. Eleven other questions ('self-rated health status', 'deep sleep', 'smoking', 'frequency of drinking', 'stress levels', 'health-related fitness levels', 'pounding of the heart', 'strange feelings on the skin', 'interfere with daily life', 'menopause will bring you a chance to see the life in a different perspective', and 'body mass index') were selected as valid questions. For the response scale for each question, 5 point Likert scale was used to make total 100 point score. Conclusions: This study is the first attempt to develop a health status assessment tool for middle aged women based on their dietary patterns. We conclude that this tool is expected to be a useful and practical tool in the field.