• Korean Journal of Microbiology
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• v.46 no.1
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• pp.86-92
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• 2010

Thirty-one chicken feather-degrading bacteria were isolated from wasted feather, compost and wastewater in a chicken farm. These isolates were categorized as Firmicutes (21 strains), ${\gamma}$-proteobacteria (4 strains), Actinobacteria (4 strains), and Bacteroidetes (2 strains) by 16S rRNA gene sequence analysis. We examined the feather-degrading isolates for degradation in the 2% of chicken feather meal. The strain Chryseobacterium sp. FBF-7, Stenotrophomonas maltophilia FBS-4, and Lysinibacillus sp. FBW-3 were selected as a keratinolytic protein degrading bacteria which showed the highest feather degradation of 75-90%. The characteristics of amino acids extracted from chicken feather meal by using keratinolytic protein degrading isolates and chemical method with $Ca(OH)_2$ were analyzed. Total amino acid content of strain Chryseobacterium sp. FBF-7 was 1,661.6 ${\mu}mol$/ml, which was the highest and it was similar with chemical method. And essential amino acid content of total amino acid was thirty-seven percent (619.3 ${\mu}mol$/ml) and 596.9 ${\mu}mol$/ml for keratinolytic protein degrading isolates and chemical method, respectively. The major amino acids were valine, glutamic acid, aspartic acid, glycine, and proline by the strain Chryseobacterium sp. FBF-7 and especially, higher contents of aspartic acid, threonine, serine, cysteine, and tyrosine were detected compared with chemical method.

• Microbiology and Biotechnology Letters
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• v.34 no.2
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• pp.180-184
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• 2006

Nineteen nitrogen-fixing bacteria were isolated from rice roots cultivated in the southern part of the Korean peninsula. Among them, three isolates - Stenotrophomonas sp. KNUC89, Pseudomonas sp. KNUC116, and Delftia sp. KNUC133 - showed antifungal activities against phytopathogenic fungi Rhizoctonia solani and Fusarium oxysporum. Besides they could produce auxin and siderophores.

• 한국약용작물학회:학술대회논문집
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• 2008.11a
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• pp.217-218
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• 2008

• The Plant Pathology Journal
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• v.34 no.3
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• pp.208-217
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• 2018

In this study, samples were collected from the leaves and stems of healthy wild Pistachio trees (Pistacia atlantica L.) from various locations of Baneh and Marivan regions, Iran. In total, 61 endophytic bacteria were isolated and grouped according to phenotypic properties. Ten selected isolates from each group were further identified by partial sequencing of the 16S rRNA gene. Based on the results, isolates were identified as bacteria belonging to Pseudomonas, Stenotrophomonas, Bacillus, Pantoea and Serratia genus. The ability of these isolates was evaluated to phytohormone production such as auxin and gibberellin, siderophore production, phosphate solubilization, atmospheric nitrogen fixation, protease and hydrogen cyanide production. All strains were able to produce the plant growth hormone auxin and gibberellin in different amounts. The majority of strains were able to solubilize phosphate. The results of atmospheric nitrogen fixation ability, protease and siderophore production were varied among strains. Only Ba66 could produce a low amount of hydrogen cyanide. The results of biocontrol assay showed that Pb78 and Sp15 strains had the highest and lowest inhibition effects on bacterial plant pathogens, Pseudomonas syringae pv. syringae Pss20 and Pseudomonas tolaasii Pt18 under in vitro condition. Pb3, Pb24 and Pb71 strains significantly promote root formation on carrot slices. To our knowledge this is the first report of the isolation of endophytic bacterial strains belonging to Pantoea, Bacillus, Pseudomonas, Serratia and Stenotrophomonas genus from wild pistachio trees with plant growth promoting potential and biocontrol activity.

• Journal of Microbiology and Biotechnology
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• v.13 no.1
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• pp.70-76
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• 2003

The benzene removal characteritics of the polyurethane (PU) biofilter immobilized with S. maltophilia T3-c, that could efficiently degrade benzene, was investigated. Maximum capacity to eliminate benzene was maintained at $100-110g{\cdot}m^-3{\cdot}h^-1$ when space velocity (SV) ranged from 100 to $300 h^-1$ -1/, however, it decreased sharply to $55 g{\cdot}m^-3{\cdot}h^-1^$ as SV increased to $400 h^-1$. The critical elimination capacities that guaranteed $90\%$ removal of inlet loading of the PU biofilter were determined to be 70,30, and $15 g{\cdot}m^-3{\cdot}h^-1$ at SV 100,200, and $300 h^-1$, respectively. Based on the result of a kinetic analysis of the PU biofilter, maximum benzene elimination velocity ($V_m$) was $125 g{\cdot}m^-3^\;of\;PU{\cdot}h^-1$ and saturation constant ($K_m$) was $0.22 g{\cdot}m^-3^$ of benzene ($65{\mu}{\cdot}I^-1$). This study suggests that the biofilter utilizing S. maltophilia T3-c and polyurethane is a very promising technology for effectively degrading benzene.

• Microbiology and Biotechnology Letters
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• v.34 no.1
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• pp.78-82
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• 2006

Since there could be more and rather various diazotrophs in rhizosphere of wild crops than those in rhizosphere of cultivars, some wild gramineous crops grown in Korea were collected for isolating nitrogen-fixing bacteria. Six diazotrophs were purified from their roots using nitrogen-free media. The isolated bacteria were partially identified as 4 genera by 16S rDNA sequence analysis: Stenotrophomonas sp., Bosea sp., Klebsiella sp., and Azorhizobium sp. By PCR amplification and sequence analysis, DNA fragments extracted from all isolates turned out to have an individual nifH homologous gene. Five isolates (KNUC163, KNUC165, KNUC169, KNUC170, and KNUC171) showed auxin activity and four isolates (KNUC163, KNUC166, KNUC170, and KNUC171) produced siderophores. Especially,3 strains of S. maltophilia showed both auxin and siderophore activities. In conclusion, the isolated nitrogen-fixing bacteria might have capabilities for plant growth promotion.

• Journal of Environmental Science International
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• v.23 no.1
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• pp.129-142
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• 2014

To treat chromaticity contained in effluents of dyeing wastewater efficiently, potent dye-degrading microorganisms were isolated from influent water, aeration- tank sludge, recycle water and settling-tank sludge located in leather and dyeing treatment plant. Six potent strains were finally isolated and identified as Comamonas testosteroni, Methylobacteriaceae bacterium, Stenotrophomonas sp., Kluyveromyces fragilis, Ascomycetes sp. and Basidiomycetes sp. When Basidiomycetes sp. was inoculated into ME medium containing basal mixed-dyes, 93% of color was removed after 8 days incubation. In the same experiment, the 1:1 mixed culture of Basidiomycetes sp. and photosynthetic bacterium exhibited 88% of color removal; however, it showed better color removal for single-color dyes. The aeration-tank and settling-tank samples revealed higher color removal (95-96%) for black dyes. The settling-tank sample also revealed higher color removal on basal mixed-dyes, which resulted in 90% color removal after 6-h incubation. From the above results, it is expected to achieve a higher color removal using the mixed microorganisms that were isolated from aeration-tank and settling-tank samples.

• Microbiology and Biotechnology Letters
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• v.33 no.1
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• pp.70-73
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• 2005

A soil bacterium was isolated from maize roots cultivated in Korea (KNUC153). The isolate was partially classified on basis of l6S rDNA sequence analysis as Stenotrophomonas maltophilia. By the acetylene reduction assay (ARA), the strain KNUC153 contained nitrogen-fixing abilities. The amount of auxin produced by the strain KNUC153 was $77.6\;{\mu}g/ml$. The strain KNUC153 produced 4 times higher amount of l-amino-cyclopropane-l-carboxy­lic acid deaminase than that of the other known strain Azospirillum sp. KNUC82. Inoculation treatment with the strain KNUC153 for maize seeds showed positive effect on early growth of the plants.

• Korean Journal of Veterinary Research
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• v.51 no.3
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• pp.243-247
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• 2011

It is important to identify the bacteria in snakes because they can cause disease; importantly, bacteria such as Stenotrophomonas maltophilia, Escherichia coli, Proteus vulgaris etc. could be pathogens especially in hospitalized, debilitated hosts, and immunocompromised patients. To analyze the distribution of snakes' bacteria in petting zoo, samples from 20 snakes were collected from 2002 to 2008. Nine bacteria species were isolated from both oral and cloaca while four and six species were identified only from oral and cloaca, respectively. Except for Actinobacter sp., all of the identified strains are opportunistic pathogens, and most of them can cause nosocomial infections in humans. Present results indicate that prevalence of various zoonotic bacterial strains in snakes could be involved in potential transfer of these bacteria into caretakers and other animals. Therefore, it needs to examine the antibiotic resistance of these pathogens to prevent outbreaks.

• Journal of Food Hygiene and Safety
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• v.28 no.4
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• pp.306-311
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• 2013

This study was conducted to investigate spoilage bacteria on organic and conventional fresh produce in Korea. Three samples (perilla leaf, cabbage, and romaine lettuce) of organic and conventional fresh produce were stored at $4^{\circ}C$ for 14 days and examined for spoilage bacteria on TSA. Isolated bacteria from organic and conventional fresh produces were identified using 16S rRNA sequencing method. Population of total aerobic bacteria on conventional perilla leaf, cabbage, and romaine lettuce were 7.59, 7.01, and $5.84{\log}_{10}CFU/g$, and populations of total aerobic bacteria were 6.72, 6.15, and $5.85{\log}_{10}CFU/g$, for organic perilla leaf, cabbage, and romaine lettuce, respectively. Major spoilage bacteria of organic and conventional fresh produces were similar however their levels were little different. For example, a major spoilage bacterium resulting the highest level on conventional perilla leaf was Stenotrophomonas maltophilia whereas that was Microbacterium sp. for organic produce. From these results, microflora or spoilage microorganism could be different depending on their cultivation types as conventional or organic produces and this information might be used for developing effective preservation method for different types of fresh produce.