• Journal of Bio-Environment Control
• /
• v.6 no.1
• /
• pp.15-25
• /
• 1997

Plug seedlings of bell pepper(Capsicum annuum L.) were grown for 50 days in controlled environment chambers under 12 hrs per day photoperiodic condition with sixteen different day and night temperature regimes to investigate the possibility of height control. The seedlings were then transplanted to greenhouse to investigate the growth, flowering, and yield afterward. Plant height and stem length of seedlings were mainly affected by day temperature rather than night temperature. Internode elongation was suppressed by a negative DIF and was enhanced by a positive DIF even with the same average daily temperature (ADT). Leaf unfolding rate was influenced more by ADT than by DIF. Fresh and dry weights increased as ADT increased. Leaf area and stem diameter increased until temperature increased up to 24$^{\circ}C$ day and night temperature and decreased above 24$^{\circ}C$, The position at which the first flower was initiated was lowered as ADT increased. The first flower degeneration was not obvious up to 24$^{\circ}C$ ADT but increased rapidly above 24$^{\circ}C$ ADT. Seedling compactness(Dry weight per plant height :mg.mm$^{-1}$ ) was greater under -DIF than +DIF condition. In conclusion, DIF treatment was an applicable technique to control stem elongation and growth rate such as leaf unfolding rate and position at which first flower was initiated could be controlled by ADT.

• Journal of agriculture & life science
• /
• v.43 no.1
• /
• pp.1-8
• /
• 2009

This study was conducted to examine the biomass, allometric equations, net primary production, above and total biomass expansion factors and stem density values for 27 years old Korean pine(Pinus koraiensis Siebold et Zuccarini) plantation at the Gangneung National Forest. After considering of the diameter distributions in the $20m{\times}20m$ plot measurement, a total of 5 representative sample trees were destructively sampled to measure green weights and dry weights of the four(root, stem, branch and foliage) protions of Korean pine trees. According to the results of this study, total dry weights were 117.6 kg/tree and 59.9 ton/ha. Aboveground biomass and total (above and belowground) biomass for this species were 59.9 and 82.4 ton/ha, respectively. Ratios of root to aboveground biomass were 0.38. Net primary production of aboveground biomass and belowground biomass were 9.4 and 11.3 ton/ha, respectively. Stem density was $0.49g/cm^{3}$. Above and total biomass expansion factors were 1.78 and 2.19, repectively. This information could be very useful to calculate carbon sequestrations by applying stem desity values and biomass expansion factors for Korean pine species.

• Tissue Engineering and Regenerative Medicine
• /
• v.15 no.6
• /
• pp.735-750
• /
• 2018

BACKGROUND: The major challenge of tissue engineering is to develop constructions with suitable properties which would mimic the natural extracellular matrix to induce the proliferation and differentiation of cells. Poly(${\varepsilon}$-caprolactone)-poly(ethylene glycol)-poly(${\varepsilon}$-caprolactone) (PCL-PEG-PCL, PCEC), chitosan (CS), nano-silica ($n-SiO_2$) and nano-hydroxyapatite (n-HA) are biomaterials successfully applied for the preparation of 3D structures appropriate for tissue engineering. METHODS: We evaluated the effect of n-HA and $n-SiO_2$ incorporated PCEC-CS nanofibers on physical properties and osteogenic differentiation of human dental pulp stem cells (hDPSCs). Fourier transform infrared spectroscopy, field emission scanning electron microscope, transmission electron microscope, thermogravimetric analysis, contact angle and mechanical test were applied to evaluate the physicochemical properties of nanofibers. Cell adhesion and proliferation of hDPSCs and their osteoblastic differentiation on nanofibers were assessed using MTT assay, DAPI staining, alizarin red S staining, and QRT-PCR assay. RESULTS: All the samples demonstrated bead-less morphologies with an average diameter in the range of 190-260 nm. The mechanical test studies showed that scaffolds incorporated with n-HA had a higher tensile strength than ones incorporated with $n-SiO_2$. While the hydrophilicity of $n-SiO_2$ incorporated PCEC-CS nanofibers was higher than that of samples enriched with n-HA. Cell adhesion and proliferation studies showed that n-HA incorporated nanofibers were slightly superior to $n-SiO_2$ incorporated ones. Alizarin red S staining and QRT-PCR analysis confirmed the osteogenic differentiation of hDPSCs on PCEC-CS nanofibers incorporated with n-HA and $n-SiO_2$. CONCLUSION: Compared to other groups, PCEC-CS nanofibers incorporated with 15 wt% n-HA were able to support more cell adhesion and differentiation, thus are better candidates for bone tissue engineering applications.

• Journal of Mushroom
• /
• v.18 no.4
• /
• pp.297-302
• /
• 2020

Five varieties of mushrooms, including Sae-do, were examined using farm field tests to determine their morphological characteristics and quality by performing harvest flush. Considering the cultivation characteristics of the varieties studied, the parts related to mushroom generation and production showed almost similar characteristics. Overall, the mycelial forces were similar; however, in the evaluation of plantation tests performed by farmers, "saedo" was found to be the strongest and "saehan" was considered to be the weakest. Weights of fruiting bodies were related more for compost status and regimen of use than for breeding, and it was difficult to distinguish between the weights of fruiting bodies of the varieties studied. Regardless of the variety, pileus diameter, pileus thickness, and stem length of Dalseong-gun, Yongin-si, and Boryeong-si decreased as the harvest flush progressed. However, stem thickness tended to increase in the second and third flush compared to that in the first flush, regardless of the variety. However, the Gyeongju-si farms could not identify any trends attributable to varieties and harvest flushes. The pileus size and stem length of the fruiting bodies of varieties from Buyeo farms decreased with the progression of the harvest flush. However, pileus thickness and stem thickness did not show any specific tendency of growth among the varieties studied. The differences in the morphological characteristics of mushroom varieties in each harvest cycle are expected to be caused by differences in the supply of nutrients in the medium, the quality of the compost used, and the cultivation environment rather than the variety.

• Journal of Plant Biotechnology
• /
• v.49 no.2
• /
• pp.145-149
• /
• 2022

Airborne fine dust (FD) particles smaller than 10 ㎛ in diameter (PM10) are one of the major causes of air pollution in East Asia, including Korea, and have become a major contributor to respiratory and skin problems. FD inordinately promotes the production of reactive oxygen species and inflammatory response in macrophages, leading to cell damage and death. Rosa rugosa, a deciduous shrub of the Rosa genus, has been used in traditional East Asian herbal medicine to treat various illnesses. The present study investigated the anti-inflammatory effects of R. rugosa organ extracts on PM10-stimulated RAW264.7 macrophages. Compared to non-treated RAW264.7 cells, treatment with 100 ㎍.ml^-1 PM10 resulted in increased nitric oxide (NO) production, similar to lipopolysaccharide treatment. Additionally, 100 ㎍/ml stem extract reduced NO production by more than 45% compared to mock treatment. Furthermore, PM10-induced expression of interleukin (IL)-1β, IL-6, inducible NO synthase, and cyclooxygenase-2 was significantly reduced by stem extract treatment, indicating that the anti-inflammatory effect of the stem extract is mediated by the inhibition of pro-inflammatory mediators in PM10-stimulated RAW 264.7 cells. These results indicate that the R. rugosa stem could be considered a natural remedy with a protective effect against inflammatory responses induced by harmful airborne dust.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.6 no.1
• /
• pp.41-49
• /
• 2004

This study was carried out to investigate the effects of temperature and photoperiod on the growth of Fagopyrum tataricum. It showed a tendency to promote the germination rate and plant height of tatary buckwheat under high temperature. Plant height and number of leaves were promoted in the order of 25>20>15>30℃ under the different temperature after 30 days of the planting. In the 10, 12, 14hr photoperiods at 20℃, plant height, number of leaves and dry weight were increased as much as photoperiod became long except by 16hr. Rutin contents was not regular both common and tatary buckwheat under the photoperiods. The rutin content in leaves was higher than stem. Compared to yield of tatary and common buckwheat, plant height, number of leaves, number of branch and stem diameter of tatary buckwheat increased more than common buckwheat. Especially, number of leaves was increased about 2.5 times more than common buckwheat. And there was a difference in about 2.1 times in grain weight and about 5.4 times in number of grains. Rutin content of plant parts was higher in the order of leaf>stem>grain in common buckwheat at the harvest. But it was higher with order of leaf>grain>stem in tatary buckwheat. Rutin content was 1469.8mg/100g in grain of tatary buckwheat. It was about 60 times higher than 22mg/100g in grain of common buckwheat.

• The Plant Pathology Journal
• /
• v.22 no.3
• /
• pp.230-234
• /
• 2006

Tomato spotted wilt virus (TSWV) has been identified in commercial chrysanthemum cultivars in Korea. Nucleotide sequences of the N gene of TSWV-ch14 isolated from infected chrysanthemum were determined and deposited in GenBank under accession no. DQ453158. The symptoms consisted of dark colored leaf necrosis, black streaks along the stem, wilting of plant parts in 'Sinma'; and chlorotic spots, necrosis of axillary shoots and withering of leaves in 'Hwarang'. Electron micrographs of leaf preparation of Nicotiana rustica infected with TSWV-ch14 contained spherical particles around 85 nm in diameter. TSWV was identified from chrysanthemum by sequence determination of N nucleocapsid protein and virion observation by transmission electron microscope. This is the first reported observation on TSWV in chrysanthemum in Korea.

• Korean Journal of Organic Agriculture
• /
• v.14 no.3
• /
• pp.325-331
• /
• 2006

The experiment fields consisted of five plots as follows; 2, 4, and $8\;tons{\cdot}ha^{-1}$ citrus skin in combination with starch sludge and pig manure mixing compost (CSSP), $4\;ton{\cdot}ha^{-1}$ fermented pig manure compost (FPMC) treated plot, and untreated control. Plant height and stem diameter were significantly increased by CSSP. Most of all, average tuber weight and tuber yield per plant were significantly increased in 4 and $8\;tons{\cdot}ha^{-1}$ CSSP plots compared to the other plots. Marketable tuber (>50 g fresh weight) yield were superior in order of 4 and $8\;tons{\cdot}ha^{-1}$ CSSP plot, $4\;tons{\cdot}ha^{-1}$ FPMC plot, and $2\;tons{\cdot}ha^{-1}$ CSSP plot.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.15 no.1
• /
• pp.67-72
• /
• 1995

To select the highest yield soiling type forage, the yield performance of selected sorghum-sudangrass hybrids imported in 1992 have been evaluated at Chonnam region. The soiling type hybrids used in this experiment were TE-haygrazer, FP-5 Sudan, Sweet sioux V, and X -78050. Three different cuttings were done on July 6, August 6 and September 16 in 1992. This trial was arranged as a randomized complete block design with three replications under taken over a period 5 months from May to September in 1992. The results of this experiment were summarized as follows: The hybrids tested were not significantly different by plant height, leaf blade length, leaf blade width and stem diameter. In the first cutting, dry matter yield of FP-5 Sudan was the highest. In the second and third cutting, dry matter yield of Sweet sioux V was the highest among hybrids. The contents of crude protein, hemicellulose, relative feeding value(RFV), and amino acid were the highest by the first cutting, but NDF and ADF were shown the lowest.

• Journal of Sericultural and Entomological Science
• /
• v.39 no.2
• /
• pp.101-105
• /
• 1997

Most of the mulberry saplings in Korea have been produced by grafting. But the production of mulberry sapling by this method is very complex process and requires high level of technique and two years to produce saplings. Therefore, to develop the mulberry sapling production system by softwood cutting method which is suitable for mass production, several conditions on rooting and growth of cuttings were investigated. The rooting ability of cuttings varied according to mulberry varieties, showing the highest rooting ratio of 93.3% in cultivar of Shingwangppong. Of different soil texture as for rooting media, the clay was found to be the best; other media decreased in order as follows; sand, sand loam and vermiculite. The shading ratio by 70% with polyethylene film showed the highest rooting ratio of the cuttings. Optimum day of cutting was around 50th day after sprouting, whereas the ratio of stem thicker than 7mm at the base of saplings in diameter was highest at 44th day.