• 제목/요약/키워드: Stem Cuttings

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Migrations and Multiplications of Bursaphelenchus xylophilus and B. mucronatus in Pinus thumbergii in Relation to Their Pathogenicity

  • Son, Joung A;Moon, Yil-Sung
    • The Plant Pathology Journal
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    • 제29권1호
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    • pp.116-122
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    • 2013
  • To evaluate the mechanisms of pathogenicity and nonpathogenicity of Bursaphelenchus xylophilus and B. mucronatus isolated in South Korea, we used 4-year-old P. thunbergii seedlings and 20-cm long one-year-old stem cuttings of 5-year-old Pinus thunbergii, and studied distributions and multiplications of pine wood nematodes after inoculation. The distributions of B. xylophilus in the 20-cm pine stem cuttings were not significantly different from that of B. mucronatus. Conversely, the proliferation rate of B. xylophilus on mycelial mats of Botrytis cinerea was significantly different from that of B. mucronatus. The study using 4-year-old P. thunbergii seedlings also showed that B. mucronatus can migrate to distal portions of the pine seedlings the same as B. xylophilus, but the populations of B. xylophilus remaining in the pine seedlings were relatively larger than those of B. mucronatus. Therefore, we concluded that the pathogenicity of B. xylophilus could be strongly influenced by its ability to multiply.

Mass Propagation of Plug Seedling using Stem Cutting and Their Tuber Yield in Potato

  • Park, Yang-Mun;Song, Chang-Khil;Kang, Bong-Kyoon;Kim, Dong-Woo;Ko, Dong-Hwan
    • 한국작물학회지
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    • 제44권3호
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    • pp.201-206
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    • 1999
  • For the mass production of plug seedlings in cultivar ‘Dejima’ potato (Solanum tuberosum L.) the optimal apical cutting diameter for rooting and rapid multiplication of stem cuttings in hydroponics were determined. In addition, the best planting date was predicted to increase tuber yield of plug seedlings at fall cropping in Cheju-Do, Korea. Days to initial rooting decreased as the cutting diameter was reduced. Plant height, leaf number, root length and root weight per plant were favorable as the cutting diameter was small. The ideal cutting diameter was 1-2 mm in this experiment. In the hydroponic cultures, the Japanese standard (JS) nutrient solution was the most effective for multiplication of stem cuttings. It was able to propagate more than 20 times a month from a single mother plant. Viability of plants, which were derived from plug seedlings using stem cuttings, was excellent when transplanted to the field. The number of tubers and tuber yield in both of the plug seedlings and seed potato planting plots were high when planted on 25 August. The number and yield were reduced when planted on 15 August, 5 September and 15 September. The degree of decrease of tuber yield in the plug seedling planting plot however, was lower than that of seed potatoes when the planting date was late. In the case of small tubers (under 30 g), the number of tubers and tuber yield were evidently increased in the seed potato tuber planting plot; the yield of large tuber (over 80g) in the plug seedling planting plot was higher than that of the seed potato. The total tuber yield per plant in the plug seedling planting plot was less than that of the seed potato; therefore, in order to increase tuber yield it was necessary to increase field plant density.

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한국 자생 때죽나무의 조경수 이용을 위한 번식방법에 관한 연구 (Seed propagation and softwood cutting of native styrax japonicus for landscape tree uses)

  • 권오준;심경구;하유미
    • 한국조경학회지
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    • 제25권2호
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    • pp.9-19
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    • 1997
  • These studies were carried out to investigate seed propagation method and conducted using stem cuttings of Styrax japonicus for using as a woody landscape plant. The results are obtained as follows. After the H\sub2\SO\sub 4\ and GA\sub 3\ treatment to the seeds of Styrax japonicus, three months of warm treatment followed by cold stratification for four months increased seed germination rate. The rooting rate of Styrax japonicus was increased by the treatment with concentrated IBA, especially highest at 7,000ppm. Rooting percentages were highest for cuttings taken on July 15, 1995. Therefore, the most effective method for rooting of Styrax japonicus was treatment with 7,000ppm IBA on July 15 cuttings, which showed rooting rate of over 90%. Half-leaf-remained cuttings were more effective than those with two leaves on rooting of Styrax japonicus. Dipping for 10 seconds in IBA solution resulted in rooting rate of over 70%. The longer the treatment time the worse the rooting.

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Effect of Rootstock on Rooting and Early Yield of Stenting-propagated Cut Roses

  • Park, Yoo Gyeong;Jeong, Byoung Ryong
    • 원예과학기술지
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    • 제33권1호
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    • pp.11-17
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    • 2015
  • The study was conducted to investigate the effect of rootstock on growth and early yield of stenting-propagated cut roses (Rosa hybrida Hort.) 'Pink Aurora' and 'Yellow King'. The scions, prepared as single-node cuttings, each with a five-leaflet leaf, were grafted onto cuttings of Rosa indica 'Major', Rosa multiflora 'Chille Wonye No. 1', Rosa multiflora 'K-1', or Rosa multiflora 'Burr' as the rootstock. The rootstock cuttings were removed of all leaves and buds before grafting. The base of scion and the top of rootstock were held together and simultaneously cut at a $45^{\circ}$ angle for ease of grafting. Scion-rootstock unions were stuck in rockwool cubes and placed on a misted glasshouse bench for rooting before being transplanted into a rockwool slabs for cultivation. Rooting was the greatest in the 'Pink Aurora' and 'Yellow King' grafted on the rootstock Rosa indica 'Major'. In 'Pink Aurora', stem length, stem diameter, five-leaflet leaves per stem, and stem fresh weight of the harvested cut flowers were not affected by the rootstock. The greatest total yield of 'Pink Aurora' was obtained in plants grafted onto the Rosa indica 'Major' rootstock. Overall growth of 'Yellow King' was the greatest in plants grafted onto Rosa multiflora 'Burr' rootstock, although total yield was not affected by the rootstock. These results suggest that Rosa indica 'Major' is the most effective rootstock not only for rooting, but also for early yield and growth for stenting propagation of these cut roses.

시기별 적엽이 저농약 '원황'배의 과실품질과 삽수의 기내 재생장에 미치는 영향 (Fruit Quality of 'Wonhwang' Pear Trees with Low-pesticides and In Vitro Regrowth of Stem Cuttings as Affected by Time of Defoliation)

  • 김병삼;조경철;마경철;윤봉기;정석규;한점화;최현석
    • 한국유기농업학회지
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    • 제23권3호
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    • pp.469-480
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    • 2015
  • 본 연구는 저농약 수준으로 관리된 '원황'배(Pyrus pyrifolia Nakai)나무의 시기별 적엽처리가 과실품질과 기내조건에서 삽수의 재생장에 어떠한 영향을 미치는 지를 조사하기 위하여 실시되었다. 시기별 적엽처리는 8월 상순, 8월 하순, 9월 상순 처리와 대조구(무처리)를 포함하였고, 전체 잎에서 40%를 균일하게 적엽처리 하였다. 9월 상순 적엽처리와 대조구에서 도장지 생장이 증가되었고 1년생 가지의 탄수화물과 전질소 그리고 유리당 함량이 가장 높게 관찰되었다. 위의 두 가지 처리에서 과실수량과 평균과중이 가장 높았고, 과실당도와 과피색 $a^*$는 8월 하순과 9월 상순 적엽처리에서 가장 높았다. 8월 상순 적엽처리는 $-18^{\circ}C$의 기내조건에서 삽수의 전해질 누출률이 가장 높았다. 삽수의 발아율은 $-18^{\circ}C$$-21^{\circ}C$의 기내조건에서 적엽처리 간에 차이가 없었고, $-27^{\circ}C$에서는 9월 상순 적엽처리와 대조구에서 삽수의 발아율이 가장 높았다. 이에 따라 강우와 병충해에 의한 과총엽의 낙엽이 8월 하순까지는 최소화 되도록 과원 관리가 이루어져야 할 것으로 판단된다.

단기순환림 생산을 위한 삽목 이식기 개발 (Development of Stem-cutting Transplanter for Short-term Rotation Coppice)

  • 김동화;김대철;김상헌;신범수
    • Journal of Biosystems Engineering
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    • 제35권1호
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    • pp.37-45
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    • 2010
  • Since SRC (Short-term Rotation Coppice) such as poplar and willow can be harvested in three years, they are known to be a potential forest biomass as fuel for a power plant. The production system including transplanting and harvesting is, however, necessary to be mechanized because such a biomass should be handled in a massive volumetric size. A pull-type stem-cutting transplanter was developed in the research as the first step to realize the production of SRC. A needle-like transplanting device pushes a stem-cutting into the prepared soil bed by a pneumatic cylinder, and another device firms soil around a stem-cutting transplanted. Since this is an intermittent operation, it was necessary to develop a zero horizontal velocity mechanism which enabled only the transplanting needle part to continue a zero horizontal movement relative to the ground during the transplanting operation even when the tractor kept moving forward. The 2-row transplanter can transplant stem-cuttings at the rate of 6.5 seconds per row without missing a single attempt. The planting depth and distance were well maintained and controlled. Their CVs were between 2.1~3.4% and 0.87~1.7% for the depth and the distance, respectively. Although, the transplanted stem-cuttings tended to lean outward from the back-view and forward from the side view, they were planted within the range of $3^{\circ}$ from the upright position.

온도(溫度)와 생장조절물질(生長調節物質)이 무궁화(Hibiscus syriacus ''Honghwarang') 삽수의 맹아(萌芽)에 미치는 영향 (Effects of Temperature and Plant Growth Regulators on Bud Sprouting of Stem Cutting of Hibiscus syriacus 'Honghwarang')

  • 백이화;조근호;박천호;허무룡;곽병화
    • 원예과학기술지
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    • 제16권3호
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    • pp.355-357
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    • 1998
  • 무궁화 (Hibiscus syriacus 'Honghwarang')의 휴면특성을 구명하고자 온도와 생장조절물질을 처리하여 맹아(萌芽) 차이를 비교하였다. 맹아는 온도가 높을수록 빨랐으며($10{\sim}30^{\circ}C$), $30^{\circ}C$ 무처리구는 삽목 후 4일만에 맹아하기 시작하여 6일경에는 100% 맹아했다. GA와 BA처리도 대조구인 무처리와 큰 차이 없이 $20^{\circ}C$ 이상의 온도에서는 6일만에 맹아하여 62.5%의 높은 맹아율을 나타내었다. ABA 침지처리는 $25^{\circ}C$이하의 온도에서는 맹아율이 저조하였으나, $25^{\circ}C$이상의 온도에서는 삽목 6일 후에 맹아하기 시작하여 12일 후에 93% 맹아되어, 고온 조건에서는 생장억제제인 ABA처리가 맹아억제에 전혀 효과가 없었다.

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A Simple and Rapid Method for Functional Analysis of Plant Growth-promoting Rhizobacteria Using the Development of Cucumber Adventitious Root System

  • Bae, Yeoung-Seuk;Park, Kyung-Seok;Lee, Young-Gee;Choi, Ok-Hee
    • The Plant Pathology Journal
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    • 제23권3호
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    • pp.223-225
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    • 2007
  • Many plant growth-promoting rhizobacteria (PGPRs) have been known for beneficial effects on plants including biological control of soilborne pathogens, induced systemic resistance to plant pathogens, phytohormone production, and improvement of nutrient and water uptake of plants. We developed a simple and rapid method for screening potential PGPR, especially phytohormone producing rhizobacteria, or for analyzing their functions in plant growth using cucumber seedling cuttings. Surface-sterilized cucumber seeds were grown in a plastic pot containing steamed vermiculite. After 7 days of cultivation, the upper part 2 cm in length of cucumber seedling, was cut and used as cucumber cuttings. The base of cutting stem was then dipped in a microcentrifuge tube containing 1.5ml of a bacterial suspension and incubated at $25^{\circ}C$ with a fluorescent light for 10 days. Number and length of developed adventitious roots from cucumber cuttings were examined. The seedling cuttings showed various responses to the isolates tested. Some isolates resulted in withering at the day of examination or in reduced number of roots developed. Several isolates stimulated initial development of adventitious roots showing more adventitious root hair number than that of untreated cuttings, while some isolate had more adventitious root hair number and longer adventitious roots than that of untreated control. Similar results were obtained from the trial with rose cuttings. Our results suggest that this bioassay method may provide a useful way for differentiating PGPR's functions involved in the development of root system.

뽕나무 묘목생산을 위한 신소삽목 조건 구명 (Studies on the Conditions of Softwood Cutting for Production of Mulgerry Sapling)

  • 구태원;성규병
    • 한국잠사곤충학회지
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    • 제39권2호
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    • pp.101-105
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    • 1997
  • Most of the mulberry saplings in Korea have been produced by grafting. But the production of mulberry sapling by this method is very complex process and requires high level of technique and two years to produce saplings. Therefore, to develop the mulberry sapling production system by softwood cutting method which is suitable for mass production, several conditions on rooting and growth of cuttings were investigated. The rooting ability of cuttings varied according to mulberry varieties, showing the highest rooting ratio of 93.3% in cultivar of Shingwangppong. Of different soil texture as for rooting media, the clay was found to be the best; other media decreased in order as follows; sand, sand loam and vermiculite. The shading ratio by 70% with polyethylene film showed the highest rooting ratio of the cuttings. Optimum day of cutting was around 50th day after sprouting, whereas the ratio of stem thicker than 7mm at the base of saplings in diameter was highest at 44th day.

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겹꽃산수국의 삽목시기가 개화에 미치는 영향 (Effect of Cutting Time on Growth and Flowering of Double Flowered Hydrangea serrata for. acuminata)

  • 이종석;김현진
    • 화훼연구
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    • 제18권1호
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    • pp.33-37
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    • 2010
  • 겹꽃으로 개화하는 한국자생 산수국(Hydragea serrata for. acuminata)을 분화용 식물로 개발하기 위하여 알맞은 삽목시기를 구명하고 월별 삽목시기가 이듬해의 생육과 개화에 미치는 영향을 구명하고자 하였다. 본 실험은 2008년 3월부터 9월까지 매월 10일날에 직경 15cm 크기의 화분에 직접 삽목을 하여 재배한 결과, 초장은 5월에 삽목한 것이 31.7cm로서 가장 컷고 그 다음이 4월과 7월에 삽목한 것 이었으며 8월과 9월에 삽목한 것은 각각 23.6cm와 22.0cm로서 비슷하였다. 그러나 6월에 삽목한 것은 비정상적으로 작았고 생육도 매우 부진하였다. 잎은 8월 이후에 삽목했을때에 엽폭이 좁아지고 엽장도 짧아져서 엽면적이 감소되었으며 엽병장 또한 짧아졌으나 착엽수는 변화가 없었다. 주당 꽃송이의 수는 5월에 삽목한 것이 4송이로서 가장 많았고 7월부터 9월 사이에 삽목한 것은 3송이가 피었으며 6월에 삽목한 것은 1송이가 착화 되어서 가장 적었다. 화관의 크기는 5월에 삽목한 개체가 직경 10.3cm로서 가장 컷으며 7월이후부터는 삽목시기가 늦어질수록 화관의 크기가 감소되었다. 착화수나 꽃의 크기, 소화의 수 등의 개화 상태는 5월에 삽목한 것이 가장 양호하였고 6월에 삽목한 것이 가장 저조하였다. 그러나 8월과 9월에 삽목을 실시하더라도 이듬해에 관상가치가 있는 정상적인 꽃모양을 가진 꽃이 피었으나 개화시기는 약간 늦어졌다.