• Rapid Communication in Photoscience
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• v.3 no.2
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• pp.28-31
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• 2014

Loss of chlorophyll is the visible symptom of leaf senescence and staygreen refers to the delayed leaf senescence in plants. The staygreen gene (SGR) in rice (Oryza sativa L.) has been identified as its mutation maintains greenness during leaf senescence, and encodes a chloroplast protein required for the initiation of chlorophyll breakdown in plants. In this study, we isolated a rice SGR-homologous gene in creeping bentgrass (Agrostis stolonifera L.), and transgenic creeping bentgrass plants were obtained by introducing pCAMBIA3301 vector harboring antisense SGR gene under control of the senescence-specific SAG12 promoter. Transgenic plants were selected by herbicide resistance assays and genomic integration of the transgenes was confirmed by PCR analysis. Subsequent analyses demonstrated the staygreen phenotype of the transgenic creeping bentgrass plants with decreased chlorophyll loss during leaf senescence. These results suggest that the antisense SGR expression in creeping bentgrass delays leaf senescence, which provides a way to develop genetically engineered turfgrass varieties with the commercially useful staygreen trait.

• Molecules and Cells
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• v.38 no.5
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• pp.390-395
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• 2015

Degradation of chlorophyll (Chl) by Chl catabolic enzymes (CCEs) causes the loss of green color that typically occurs during senescence of leaves. In addition to CCEs, STAYGREEN1 (SGR1) functions as a key regulator of Chl degradation. Although sgr1 mutants in many plant species exhibit a staygreen phenotype, the biochemical function of the SGR1 protein remains elusive. Many recent studies have examined the physiological and molecular roles of SGR1 and its homologs (SGR2 and SGR-LIKE) in Chl metabolism, finding that these proteins have different roles in different species. In this review, we summarize the recent studies on SGR and discuss the most likely functions of SGR homologs.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.4
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• pp.323-328
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• 1997

Com(Zea mays L.) hybrid is recognized as the principal cmp in a double-cmpping system. Rye(Secale cereale L.), seeded after corn hybrid harvest, is the second crop in the system. Under this forage production system, corn planting is mostly delayed beyond mid-May due to delayed rye harvest on May, but the corn should be harvested until mid-August before the autumn rain started. It is for this reason that early maturing corn hybrids are preferred for silage crops in this system. Twenty eight cultivars of early maturing corn hybrids evaluated in this experiment at three locations (Suweon, Sunghwan and Cheju) for 3 yr(1994-1996) were compared for agronomic characteristics, forage yield and quality. Among the com hybrids tested, DUO1 and GL499 were recommended as the government superior corn hybrids due to their promising agronomic traits, such as short plant and ear heights, adquate dry matter percentage for silage on mid-August, good staygreen along with good lodging and disease resistance, better ear retention, similar yield compared to the control hybrid, Suweon 19 and above average stover quality.