• Title/Summary/Keyword: Stationary Phase

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Dispersal Polymorphisms in Insects-its Diversity and Ecological Significance (곤충의 분산다형성-그의 다양성과 생태학적 의의)

  • 현재선
    • Korean journal of applied entomology
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    • v.42 no.4
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    • pp.367-381
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    • 2003
  • Dispersal polymorphism in insects Is a kind of adaptive strategy of the life history together with the diapause, consisting of the “long-winged or alate forms” of migratory phase and the “short-winged or apterous forms” of stationary phase. Dispersal polymorphism is a polymorphism related with the flight capability, and has three categories ; the wing polymorphisms, flight muscle polymorphisms, and flight behavior variations. Phase variation is another type of dispersal polymorphism varying in morphology, physiology and wing forms in response to the density of the population. The dispersal migration is a very adaptive trait that enables a species to keep pace with the changing mosaic of its habitat, but requires some costs. In general, wing reduction has a positive effect on the reproductive potential such as earlier reproduction and larger fecundity The dispersal polymorphism is a kind of optimization in the evolutionary strategies of the life history in insects; a trade-off between the advantages and disadvantages of migration. Wing polymorphism is a phenotypically plastic trait. Wing form changes with the environmental conditions even though the species is the same. Various environmental factors have an effect on the dispersal polymorphisms. Density dependent dispersal polymorphism plays an important role In population dynamics, but it is not a simple function of the density; the individuals of a population may be different in response to the density resulting different outcomes in the population biology, and the detailed information on the genotypic variation of the individuals in the population is the fundamental importance in the prediction of the population performances in a given environment. In conclusion, the studies on the dispersal polymorphisms are a complicated field in relation with both physiology and ecology, and studies on the ecological and quantitative genetics have indeed contributed to understanding of its important nature. But the final factors of evolution; the mechanisms of natural selections, might be revealed through the studies on the population biology.

A Study on the Development of Analytical Methods and Behaviors of Environmental Pollutants ( I ) : Elution Behavior of Monosubstituted Phenols and Benzenes by Micellar Reversed-Phase Liquid Chromatography (환경 오염물질의 정량법 개발과 거동에 관한 연구 ( I ) : 미셀 역상 액체 크로마토그래피에서 페놀과 벤젠 일치환체들의 용리거동)

  • Lee, Dai Woon;Bang, Eun Jung;Cho, Byung Yun
    • Analytical Science and Technology
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    • v.6 no.1
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    • pp.1-8
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    • 1993
  • The purpose of this study is to investigate the elution behavior of monosubstituted phenols and benzenes in micellar liquid chromatographic system, $C_{18}$ column-anionic surfactant, sodium dodecyl sulfate(SDS). The partition coefficients between the micellar pseudophase-water and modified stationary phase-water are calculated by the relationship between solute retention and micellar mobile phase(SDS) composition. The free energy of transfer of solute from water to micelle is also calculated from these values. There is a direct correlation between the hydrophobicity parameters in MLC and corresponding partition data for 1-octanol-water, which indicates that the hydrophobicity of molecules plays an important role in the partition for both systems and that quantitative structure activity relationships(QSAR) are available from studies on micellar partition. The other purpose of this study is to investigate methylene selectivity of alkyl homologous series through correlation between retention and the number of carbons. The correlation between hydrophobicity parameters in MLC and 1-octanol-water partition data was also observed when n-propanol was as a modifier in the mobile phase.

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A Study of the Retention Mechanism of the Monosubstituted Benzenes in Reversed-phase Liquid Chromatography (II) (역상 액체크로마토그래피에서 벤젠 일치환체들의 머무름 메카니즘에 관한 연구 (제 2 보))

  • Lee, Dai-Woon;Choi, Yong-Wook;Lee, Won
    • Journal of the Korean Chemical Society
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    • v.32 no.2
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    • pp.135-143
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    • 1988
  • The correlations between chromatographic parameters of monosubstituted benzenes and several physical parameters in reversed phase liquid chromatography were studied. The relationships between retention data and polarity index were investigated by plotting $log(log k'_S/k'_B)$ vs. $P'_S/P'_B$ which were relative retention and relative polarity index of monosubstituted benzenes with respect to benzene, respectively. The linear relationship between relative retention and polarity index was observed for the monosubstituted benzenes having polar group, while in case of those having nonpolar group, the good linearity was observed by combination with relative molecular weight i.e. $(P'_S/P'_B)/(MW_S/MW_B)$. Multivariant regression analysis, $a(P'_S/P'_B)+b(MW_S/MW_B)$+c did not give significantly better correlations compared to single variant analysis, $a[(P'_S/P'_B)/(MW_S/MW_B)]$+c, but multiple stepwise regression analysis was recommended when several physical parameters simultaneously were chosen. The best correlation between retention data for monosubstituted benzenes taken from the literature and substituent constant(${\pi}$), derived from hydrophobic parameter and the first order molecular connectivity index$(^1{\chi}^{\nu})$, was established for methanol/water mobile phase system. The larger the surface coverage of the stationary phase, the higher was the correlation coefficient between these two parameters and retention data.

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Optical Resolution of Free Amino Acids with Addition of Copper(II) Chelates in a Reversed-Phase Liquid Chromatography (구리(II) 킬레이트의 첨가에 의한 자유아미노산 광학이성질체의 역상 액체크로마토그래피적 분리)

  • Sun Haing Lee;Tae Sub Oh;Hong Yeup An;Kyung Sug Park;Sang Oh OH
    • Journal of the Korean Chemical Society
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    • v.36 no.6
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    • pp.879-888
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    • 1992
  • Separation of the optical isomers of free amino acids by a reversed phase high performance liquid chromatography has been studied by adding a copper(II) complex of L-proline or L-proline derivatives (hydroxy-L-proline, N-benzyl-L-proline, p-xylenyl-L-proline, p-xylenyl-hydroxy-L-proline) in the mobile phase. An OPA postcolumn detection system was used for the detection of amino acids. The chromatographic properties for the free amino acids were discussed in terms of the pH, the kinds and concentration of chelate or organic modifier. The retention behaviors of the free amino acids were considerably different from, those of DNS-amino acids or DABS-amino acids. The enantioselectivity of the free amino acids was better than that of derivatized amino acids. The enantioselectivity between the optical isomers observed by use of the Cu(II)-p-xylenyl-L-proline chiral cheleate was the best among the several copper(II) chelate. A separation mechanism could be illustrated not only by the hydrophobic interaction of the diastereomer with stationary phase but also by the steric effect of the ligand exchange reaction between the free-amino acids and copper chelate.

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Antimicrobial Activities of Scutellariae Radix Extract against Vibrio parahaemolyticus (Vibrio parahaemolyticus에 대한 황금추출물의 항균활성)

  • 조성환;김영록
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.3
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    • pp.534-538
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    • 2002
  • To investigate the antimicrobial effects of Scutellariae Radix extract against Vibrio parahaemolyticus from food samples, Vibrio parahaemolyticus strains isolated from Tapes philippinarum were examined for their sensitivity to Scutellariae Radix extract. Total 66 Vibrio parahaemolyticus strains were isolated from Tapes philippinarm 72 samples (91.7%). The serotypes of isolated Vibrio parahemolytics were K-I group 7 strains (10.6%), K-IV group 5 strains (7.6%), K-II group 2 strains (3.0%), K-V group 2 strains (3.0%), K-VII group 2 strains (3.0%), K-VI group 1 strains (1.5%), K-VIII group 1 strains (1.5%) and antisera UT K-group 46 strains (69.7%) on antisera agglutination test, but K-III group and K-IX group strains were not found. The growth curves of isolates showed lag phase, logarithmic phase, stationary phase and death phase as typical sigmoid curve on the shellfish samples. After 6 hours, the group containing Scutellariae Radix extract differs from the control on shellfish samples in the growth inhibition curves, and Vibrio parahaemolyticus were inhibited in more than 1000 ppm Scutellariae Radix extract. The morphological changes were observed by transmission electron microscope and the microbial cells membrane was destroyed by Scutellariae Radix extract.

Antimicrobial Effects of Scutellariae Radix Extract against Listeria monocytogenes (Listeria monocytogenes에 대한 황금추출물의 항균효과)

  • 조성환;김영록
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.5
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    • pp.959-963
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    • 2001
  • To investigate the antimicrobial effects of Scutellariae Radix extract against L.monocytogenes from foods, L. monocytogenes strains isolated from livestock, processed food from meat and milk, and frozen foods, were examined for their sensitivity to Scutellariae Radix extract. 30 L. monocytogenes strains were isolated from total 178 samples(16.9%); 13(14.0%) strains from beef 6(20.7%) strains from pork, 9(39.2%) strains from chicken and 2 (16.7%) strains from frozen foods but was not found from processed products, The serotypes of isolated L.monocytogenes were serotype O-1 strains (23, 76.7%) and serotype O-4 strains(7, 23.3%) on antisera agglutination test. The growth curves of isolates were shown lag phase, logarithmic phase, stationary phase and death phase as typical sigmoid curve on the preservative-free hams. After 6 hours. Scutellariae Radix extract contain group differ from control group on preservative-free ham samples, and the isolates were inhibited in more than 1000 ppm Scutellariae Radix extract on the inhibitory growth curve of L.monocytogenes. The mor-phological changes were observed by transmission electron microscope and the microbial cells membrane was destroyed by Scutellariae Radix extract.

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Highband Coding Method Using Matching Pusuit Estimation and CELP Coding for Wideband Speech Coder (광대역 음성부호화기를 위한 매칭퍼슈잇 알고리즘과 CELP 방법을 이용한 고대역 부호화 방법)

  • Jeong Gyu-Hyeok;Ahn Yeong-Uk;Kim Jong-Hark;Shin Jae-Hyun;Seo Sang-Won;Hwang In-Kwan;Lee In-Sung
    • The Journal of the Acoustical Society of Korea
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    • v.25 no.1
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    • pp.21-29
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    • 2006
  • In this Paper a split bandwidth wideband speech coder and its highband coding method are Proposed. The coder uses a split-band approach. where the wideband input speech signal is split into two equal frequency bands from 0-4kHz and 4-8kHz. The lowband and the highband are coded respectively by the 11.8kb/s G.729 Annex E and the proposed coding method. After the LPC analysis, the highband is divided by two modes according to the properties of signals. In stationary mode. the highband signals are compressed by the mixture excitation model; CELP algorithm and W (Matching Pursuit) algorithm. The others are coded by the only CELP algorithm. We compare the performance of the new wideband speech coder with that of G.722 48kbps SB-ADPCM and G.722.2 12.85kbps in a subjective method. The simulation results show that the Performance of the proposed wideband speech coder has better than that of 48kbps G.722 and no better than that of 12.85kbps G.722.2.

Catalases in Acinetobacter sp. Strain JC1 DSM 3803 Growing on Glucose (포도당을 이용하여 성장하는 Acinetobacter sp. Strain JC1 DSM 3809에 존재하는 Catalase)

  • Shin, Kyoung-Ju;Ro, Young-Tae;Kim, Young-Min
    • Korean Journal of Microbiology
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    • v.32 no.2
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    • pp.155-162
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    • 1994
  • Cells of Acinetobacter sp. strain JC1 DSM 3803, an aerobic monoxide-oxidizing bacterium, growing on glucose exhibited high catalase activity at the mid-exponential growth phase. The enzyme activity decreased gradually after then until the early stationary phase, increased again at the mid-stationary phase, and then decreased again thereafter. Cells growing on glucose was found to contain three kinds of catalses. Cat1, Cat2 and Cat3. The activities of Cat1 and Cat3 did change significantly during growth, but that of Cat2 exhibited significant variation. Cat3 was found to present only in cells growing on glucose, but not in cells growing on carbon monoxide of methanol. The activities of call and Cat3 in cell-free extracts were stable upon treatment with ethanol and chloroform, but decreased to some extent when the enzymewere treated with 2mM $H_2O_2$ and/or 3-amino-1,2,4-triazole (AT). Cat2 was found to be extremely sensitive to the ethanol-chloroform and $H_2O_2$ treatments, but was insensitive to the AT treatment. Cat1 exhibited enzyme activity after incubation for 1 min at 80$^{\circ}C$. Cat2 and Cat3 did not show enzyme activity after incubation for 1 min at 60$^{\circ}C$ and 70$^{\circ}C$, respectively. Cat2 was found to have peroxidase activity. Cat3 was purified to homogenity in seven steps. The molecular weight of the native enzyme was estimated to be 150,000. Sodium dodecyl sulfate-gel electrophoresis revealed two identical subunits of molecular weight 65,000. The enzyme was found to show two $K_m$ values of 39 mM and 58mM. The optimal pH for the enzyme activity was 7.0, but the activities at pH 6.0, 8.0, and 9.0, were found to be comparable to that at the optimal pH. The optimal temperature for the enzyme activity was found to be 40$^{\circ}C$. The enzyme also exhibited strong activity at 20$^{\circ}C$, 30$^{\circ}C$, and 50$^{\circ}C$. The purified enzyme was not affected by the ethanol-chloroform treatment. The enzyme, howerver, showed less than 10% of the original activity when it was treated with 12 mN AT, 0.1 mM $NaN_3$ of 1mM KCN.

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Numerical Investigation of Urea Freezing and Melting Characteristics Using Coolant Heater (냉각수 순환 가열 방식을 이용한 요소수의 동결 및 해동 특성에 관한 수치적 연구)

  • Lee, Seung Yeop;Kim, Nam Il;Park, Yun Beom;Kim, Man Young
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.37 no.8
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    • pp.717-724
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    • 2013
  • Urea-SCR technology is known as one of the powerful NOx reduction systems for vehicles as well as stationary applications. For its consistent and reliable operation in vehicle applications, however, the freezing and melting of the urea solution in cold environments have to be resolved. In this study, therefore, a numerical study of three-dimensional unsteady problems was analyzed to understand the urea freezing and heating phenomena and heat transfer characteristics in terms of urea liquid volume fraction, temperature profiles, and phase change behavior in urea solutions with time by using the commercial software Fluent 6.3. As a result, it was found that the freezing phenomenon proceeds with a phase change from the tank wall to the center, whereas the melting phenomenon occurs faster in the upper part of the storage tank by natural convection and in the adjacent part of the coolant pipe than in other parts. Furthermore, approximately 190 s were required to obtain 1L of urea solution using a 4-coiled coolant heater under conditions of $70^{\circ}C$ and 200 L/h.

Change of Refractive Index of Air in X-band due to Atmospheric Humidity, Temperature and Pressure measured by GB-SAR Interferometry (GB-SAR 간섭기법으로 측정된 X-밴드 대기 굴절률의 상대습도, 기온 및 기압에 따른 변화)

  • Lee, Jae-Hee;Lee, Hoon-Yol;Cho, Seong-Jun;Sung, Nak-Hoon;Kim, Kwang-Eun
    • Korean Journal of Remote Sensing
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    • v.27 no.2
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    • pp.163-170
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    • 2011
  • In this paper, we analyzed the phase change of 5-triangular trihedral comer reflectors by using X-band Ground-Based Synthetic Aperture Radar (GB-SAR) system. Each reflector was set as a stationary target at a different distance from the system. We obtained total 123 full-polarization images during 40 hours continuously at 20 minute interval. Results of SAR interferometric analysis showed phase changes of maximum 2 radians and followed similar pattern with atmospheric data. Through a GB-SAR phase formula that includes refractive index in the air, we performed regression analysis for refractive index as a function of atmospheric humidity, temperature and pressure. As a result, refractive index of air in X-band showed relatively high coefficient of determination with humidity and temperature (0.72 and 0.76 on average, respectively) but not so with pressure (0.34). The refractive index of air in X -band changed by 3.14\;{\times}\;10^{-5}$ during the measuring time with a humidity range of 50% ~ 90% and a temperature range of $-1^{\circ}C$ ~ $9^{\circ}C$. We expect that a total expression of refractive index of air including humidity, temperature and pressure can be calculated when more extensive data would be collected at various atmospheric conditions.