• Journal of Applied Biological Chemistry
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• 제66권
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• pp.128-137
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• 2023

Transcriptome analysis revealed that the sinR gene encoding a transition-state regulator of Bacillus pumilus, genetically close to B. subtilis, was expressed at high levels during growth. The sinR gene is the second gene of the sinIR operon consisting of three promoters and two structural genes in B. subtilis. This study used the sinIR promoter of B. subtilis DB104 to construct a recombinant protein expression system. First, the expression ability depending on the number of sinIR promoter was investigated using enhanced green fluorescent protein (eGFP). The expression level of eGFP was slightly higher when using two promoters (Psin2) than using original promoters. The Psin2 promoter was further engineered by modifying the repressor binding site and -35 and -10 regions. Shine-Dalgarno (SD) sequence of the sinI gene was modified to the consensus sequence. Finally, combining the engineered Psin2 promoter with the modified SD sequence increased the expression level of eGFP by about 13.4-fold over the original promoter. Our results suggest that the optimized sinIR promoter could be used as a novel tool for recombinant protein expression in B. subtilis.

• Journal of Acupuncture Research
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• 제24권3호
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• pp.9-18
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• 2007

Objectives: Bloodletting therapeutics is one of the most popular oriental medical treatments in Korea. In this study, we operate the Interview Survey for grasping clinical actual state bloodletting therapeutics in Korea. Methods : Survey questions were developed based on consensus of acupuncture professors. The list of the Korean medical doctors with experiences more than 10 years is provided by the Association of the Korean Oriental Medicine. The interviews were conducted to 39 members of the Korean medical doctors who answered to used bloodletting therapeutics over 30 percentage a day at previous telephone survey. Interview survey with them were conducted by the well-trained interviewers of College of Korean Medicine student from 29th May 2006 to 3rd June 2006. Results : Korean medical doctors prefer to use the bloodletting cupping treatment(89.5%) on the bloodletting therapeutics. Musculo-skeletal disorder was as frequent as 89.5% of treatment disease. The most common treatment area was back(57.9%), extremity(l5.8%), pain area(l0.5%). The most common instrument for treating was disposable lancet(57.9%), three-edged needle(26.3%). Most Korean medical doctors(60.5%) took up the position that bring symptom relief following good treatment area. 'Recover quickly from illness'(50%) was one of bloodletting good points but 'Sever pain'(34.2%) was a weak points. Conclusion : This survey provides unique insight into the perception of the Korean medical doctors at bloodletting therapeutics. Most doctors experienced symptom relief, received positive benefits from the treatment. Future research needs to provide more in-depth insight into doctor views of the experience.

• 한국치위생학회지
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• 제19권6호
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• pp.891-905
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• 2019

Objectives: The objective of this study was to examine the current state of dementia-related education for dental hygiene degree programs in Korea, as well as to develop a dementia-related curriculum for dental hygiene programs suitable for the social characteristics of Korea. Methods: The study was conducted between April and November 2018. First, the current state of dementia-related education for dental hygiene programs in colleges throughout Korea and dementia-related education for other health care-related fields were investigated. Based on the initial findings, the basic content of the preliminary curriculum was constructed. Second, based on the opinion of the dementia-related curriculum development committee comprising 10 professors of dental hygiene, the operations of dementia-related courses and relevant details were constructed. Third, these operations and relevant details were assessed and revised based on focus-group interviews. Fourth, the dementia-related curriculum was developed based on the study findings and literature review. Results: The name of the course in the developed curriculum was set as "Elderly Dental Hygiene and Practicum." The course was established as a "major elective," and was offered as two units with two instructional sessions of two hours each. The learning goal for this course was acquiring the methods for understanding and managing the characteristics of the elderly. A consensus was reached regarding conducting the theoretical and practical lessons on some of the dementia-related content. The dementia-related curriculum comprised 10 "required contents" which is to be conducted over three weeks of theoretical lessons and two weeks of practical lessons. Conclusions: With the growing elderly population, the development and operation of the curriculum for geriatric oral health interventions are warranted in dental hygiene education. However, continued discussions and improvements are needed on the extent of educational content, considering various dementia-related symptoms and general geriatric systemic diseases.

• Journal of Microbiology and Biotechnology
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• 제17권4호
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• pp.604-610
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• 2007

A psychrotrophic strain 7195 showing extracellular lipolytic activity towards tributyrin was isolated from deep-sea sediment of Prydz Bay and identified as a Psychrobacter species. By screening a genomic DNA library of Psychrobacter sp. 7195, an open reading frame of 954 bp coding for a lipase gene, lipA1, was identified, cloned, and sequenced. The deduced LipA1 consisted of 317 amino acids with a molecular mass of 35,210 kDa. It had one consensus motif, G-N-S-M-G (GXSXG), containing the putative active-site serine, which was conserved in other cold-adapted lipolytic enzymes. The recombinant LipA1 was purified by column chromatography with DEAE Sepharose CL-4B, and Sephadex G-75, and preparative polyacrylamide gel electrophoresis, in sequence. The purified enzyme showed highest activity at $30^{\circ}C$, and was unstable at temperatures higher than $30^{\circ}C$, indicating that it was a typical cold-adapted enzyme. The optimal pH for activity was 9.0, and the enzyme was stable between pH 7.0-10.0 after 24h incubation at $4^{\circ}C$. The addition of $Ca^{2+}\;and\;Mg^{2+}$ enhanced the enzyme activity of LipA1, whereas the $Cd^{2+},\;Zn^{2+},\;CO^{2+},\;Fe^{3+},\;Hg^{2+},\;Fe^{2+},\;Rb^{2+}$, and EDTA strongly inhibited the activity. The LipA1 was activated by various detergents, such as Triton X-100, Tween 80, Tween 40, Span 60, Span 40, CHAPS, and SDS, and showed better resistance towards them. Substrate specificity analysis showed that there was a preference for trimyristin and p-nitrophenyl myristate $(C_{14}\;acyl\; groups)$.

• Archives of Plastic Surgery
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• 제49권2호
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• pp.184-194
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• 2022

The face and the external nose define an individual's physical appearance. Nasal deformities can cause facial disfigurement along with unwanted psychological repercussions. Nasal deformities range in severity, with the most severe cases being indications for a rhinectomy, due to the complexity of the nasal defect. According to published literature, there is no consensus among otolaryngologists and plastic surgeons on which technique or flap use is preferred in terms of complications, aesthetic outcome, or patient satisfaction. The goal of this study is to provide a comprehensive analysis of published studies on nasal reconstruction following rhinectomy. Using the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols guidelines for writing systematic reviews, a systematic review was conducted. Four databases were searched using a search strategy. These articles were then imported into the COVIDENCE software and went screening and thorough article review. After screening 2,237 articles, 23 studies were then extracted for data collection analysis. We collected data from 12 case series, 4 case studies, 1 prospective case series, and 4 retrospective chart review studies. The most commonly reported flaps were forehead flaps, superior extended nasal myocutaneous island, forearm free flaps, anterolateral thigh (ALT) free flap, medial femoral condyle free flap (n = 8), and zygomaticus implants (n = 6), and retained nasal prosthesis. Although not specifically indicated by a certain number, the most common indication for the rhinectomy was malignancy, followed by traumas, postsurgical complications, radionecrosis, and congenital nasal malformations.

• 항공우주정책ㆍ법학회지
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• 제31권2호
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• pp.237-269
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• 2016

Asia-Pacific Space Cooperation Organization (APSCO) is the only intergovernmental space cooperation organization in Asia. Since its establishment to date, eight countries have signed the convention and become member states. South Korea participated actively in the preparatory phase of creating the organization, and one conference organized by AP-MCSTA which is the predecessor of APSCO was held in South Korea. However, after the APSCO Convention was opened for signature in 2005 to date, South Korea does not ratify the Convention and become a member. The rapid development of space commercialization and privatization, as well as the fastest growing commercial space market in Asia, provides opportunities for Asian countries to cooperate with each other in relevant space fields. And to participate in the existing cooperation framework (e.g., the APSCO) by the Asian space countries (e.g., South Korea) could be a proper choice. Even if the essential cooperation in particular space fields is challenging, joint space programs among different Asian countries for dealing with the common events can be initiated at the first steps. Since APSCO has learned the successful legal arrangements from ESA, the legal measures established by its Convention are believed to be qualified to ensure the achievement of benefits of different member states. For example, the regulation of the "fair return" principle confirms that the return of interests from the relevant programs is in proportion to the member's investment in the programs. Moreover, the distinguish of basic and optional activities intends to authorize the freedom of the members to choose programs to participate. And for the voting procedure, the acceptance of the "consensus" by the Council is in favor of protecting the member's interest when making decisions. However, political factors that are potential to block the participation of South Korea in APSCO are difficult to be ignored. A recent event is an announcement of deploying THAAD by South Korea, which causes tension between South Korea and China. The cooperation between these two states in space activities will be influenced. A long-standing barrier is that China acts as a non-member of the main international export control mechanism, i.e., the MTCR. The U.S takes this fact as the main reason to prevent South Korea to cooperate with China in developing space programs. Although the political factors that will block the participation of South Korea in APSCO are not easy to removed shortly, legal measures can be taken to reduce the political influence. More specifically, APSCO is recommended to ensure the achievement of commercial interests of different cooperation programs by regulating precisely the implementation of the "fair return" principle. Furthermore, APSCO is also suggested to contribute to managing the common regional events by sharing satellite data. And it is anticipated that these measures can effectively response the requirements of the rapid development of space commercialization and the increasing common needs of Asia, thereby to provide a platform for the further cooperation. In addition, in order to directly reduce the political influence, two legal measures are necessary to be taken: Firstly, to clarify the rights and responsibilities of the host state (i.e., China) as providing assistance, coordination and services to the management of the Organization to release the worries of the other member states that the host state will control the Organization's activities. And secondly, to illustrate that the cooperation in APSCO is for the non-military purpose (a narrow sense of "peaceful purpose") to reduce the political concerns. Regional cooperation in Asia regarding space affairs is considered to be a general trend in the future, so if the participation of South Korea in APSCO can be finally proved to be feasible, there will be an opportunity to discuss the creation of a comprehensive institutionalized framework for space cooperation in Asia.

• 분석과 대안
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• 제2권1호
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• pp.31-69
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• 2018

This study examines the role of science in resolving trade disputes. After the Great East Japan Earthquake of 11 March 2011 that not only jeopardized the people of Japan, but also put the safety of an entire region at risk, the Republic of Korea (Korea) has imposed import bans as well as increased testing and certification requirements for radioactive material on Japanese food products. Japan has challenged these restrictions at the World Trade Organizations Dispute Settlement Body (DSB). This study aims to explain how international trade agreements and previous DSB rulings have dealt with different scientific viewpoints provided by confronting parties. In doing so, it will contrast the viewpoints espoused by Korean and Japanese representatives, and then analyzes the most similar case studies previously ruled on by the DSB, including the case of beef hormones and the case of genetically modified crops including biotech corn, both between the United States and the European Communities (EC). This study finds that science is largely subordinate to national interests in the case of state decision-making within the dispute settlement processes, and science has largely been relegated to a supportive role. Due to the ambiguity and lack of truly decisive decisions in the Appellate Body in science-based trade disputes, this study concludes that the Appellate Body avoids taking a firm scientific position in cases where science is still inconclusive in any capacity. Due to the panel's unwillingness to establish expert review boards as it has the power to do, instead favoring an individual-based system so that all viewpoints can be heard, it has also developed a system with its own unique weaknesses. Similar to any court of law in which each opposing party defends its own interests, each side brings whatever scientific evidence it can to defend its position, incentivizing them to disregard scientific conclusions unfavorable to their position. With so many questions that can arise, combined with the problems of evolving science, questions of risk, and social concerns in democratic society, it is no wonder that the panel views scientific information provided by the experts as secondary to the legal and procedural issues. Despite being ruled against the EC on legal issues in two previous cases, the EC essentially won both times because the panel did not address whether its science was correct or not. This failure to conclusively resolve a debate over whose science is more scientific enabled the EC to simply fix the procedural issues, while continuing to enforce trade restrictions based on their scientific evidence. Based on the analysis of the two cases of disputes, Korea may also find itself guilty of imposing an unwarranted moratorium on Japan's fish exports, only to subsequently pass new restrictions on labelling and certification requirements because Japan may have much scientific evidence at its disposal. However, Korea might be able to create enough uncertainty in the panel to force them to rule exclusively on the legal issues of the case. This will then equip Korea, like the EC in the past, with a way of working around the ruling, by changing whatever legal procedure they need to while maintaining some, if not most, of its restrictions when the panel fails to address its case on scientific grounds.

• 한국해양환경ㆍ에너지학회지
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• 제12권4호
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• pp.284-288
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• 2009

선박평형수 및 침전물 관리에 관한 국제협약은 IMO에서 2004년 외교회의를 통해 채택되었다. 선박평형수 관리협약의 준비를 위해서는 처리장치, 형식승인, 위해도 평가 및 국내 법제화를 지원하기 위한 기술 자료 등의 기반기술이 개발되어야 할 것이다. 위해도 평가를 위해서는 선박평형수 배출량에 대한 샘플링 조사를 수행하여야 한다. 선박운항 및 선박평형수 배출량 정보는 선박 및 대리점 방문을 통하여 수집하였다. 97척의 화물 적하역과 선박평형수 입배출량의 관계에 대한 선종별 특성해석을 통해, 국내 각 항만에서의 선박평형수 입배출량을 추정하였다. 이로부터 부산항, 인천항, 광양항 및 울산항에서의 선박평형수 총 흡입량은 약 7천만톤으로서, 총 배출량의 3배 이상이 되는 것으로 나타났다.

• 지역사회간호학회지
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• 제11권1호
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• pp.278-288
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• 2000

본 연구의 목적은 아동성폭력의 정의에 대한 한국간호사들의 인식의 특성을 알아보는 것이다. 보다 구체적으로, 양호교사와 병원간호사 간의 인식의 일치정도를 밝히고, 간호사들이 아동성폭력의 심각도를 판단함에 있어, 아동성폭력과 관련된 다양한 변수들의 상대적 중요성을 규명하는데 초점을 두고 있다. 이를 위하여 503명의 병원 간호사와 526명의 양호교사를 대상으로 한 vignette디자인 설문조사를 실시하였다. 아동성폭력 상황을 구성하는 일곱 개의 변수 (성폭력 행위 성폭력 빈도, 피해자의 연령, 피해자의 저항, 가해자의 연령, 가해자와 피해자의 교차성별, 가해자와 피해자의 관계)의 조합을 통해 64개의 vignettes를 만들었는데, 이는 1/4 factorial 디자인의 결과이다. 각 설문 대상자에 대해 64개의 vignettes가운데 16개의 vignettes를 무작위로 추출하고, 그 vignettes에 나타난 각 성폭력상황들의 심각 정도를 표시하도록 하였다. 연구결과에서 양호교사와 병원간호사는 아동성폭력의 심각도 인식에 있어 일치를 나타냈다. 각 상황변수들이 미치는 영향에 있어서는 양호교사와 병원간호사 간에 다소 차이를 보였으나, 성폭력 상황을 정의함에 있어서는 근본적인 인식에 일치를 보였다 두 집단 모두에서 성폭력행위 피해자의 저항, 가해자와 피해자의 교차성별, 성폭력 빈도, 가해자의 연령이 아동성폭력 인식에 영향하는 주요 요인으로 나타났다 한편, 가해자와 피해자의 관계는 주요 영향요인이 아닌 것으로 나타났다. 피해자의 연령은 양호교사 집단에서는 어느 정도 영향을 미치는 변수로, 병원간호사 집단에서는 별다른 영향이 없는 변수로 분석되었다. 결론적으로, 양호교사와 병원간호사는 잠재되어 있는 아동성폭력의 심각도에 대해 동일하게 판단한다. 본 연구결과는 법적, 전문가적 의미에서 아동성폭력 신고자로서의 의무를 지닌 한국간호사들의 아동성폭력에 대한 인식일치가, 그 역할 수행에 도움이 될 수 있음을 시사한다.

• 한국항공우주학회지
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• 제41권9호
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• pp.740-746
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• 2013

항공기, 자동차를 포함하는 모든 수송수단 설계에 있어서 내추락성 설계가 강조되고 있으나, 심각한 부상 또는 사망으로 이어지는 사고는 지속적으로 발생해 왔고, 앞으로도 발생할 것이다. 심지어 생존 가능으로 분류되는 사고에서조차도 상당한 수준의 인명사고가 있어 왔음은 주지의 사실이다. 그러나 이러한 사고들이 반드시 불가피하다고만 할 수는 없다. 만약 좌석, 구속장치, 탑승공간 강도조건 등 탑승자 보호계통이 적절히 또는 바르게 설계된다면 추락상황에서의 생존성은 획기적으로 증대될 수 있다. 이를 위해서는 급격한 가속도 변화환경에서의 인체 허용한도 특성을 충분히 이해해야 하며, 이를 바탕으로 인체 허용한도 제한치 이내에서 하중조건이 유지되도록 탑승공간을 설계하여야 한다. 본 논문에서는 급격한 가속도 변화환경에서의 인체 허용한도의 중요성과 예측되는 추락환경 변화에 따른 설계요구도 변화 필요성을 강조함으로써 내추락성 설계에 대한 공감대를 넓히고자 한다.