• Journal of Astronomy and Space Sciences
• /
• v.11 no.1
• /
• pp.21-30
• /
• 1994

VV Cep-type long period spectroscopic-eclipsing binary AZ Cas has been observed for five years, 1985 Oct~1990 Feb, UBV at the Ilsan Station of Yonsei University Observatory. A total of 431 observations (U=129, B=142 and V=160) are made for 86 nights. Instrumental differential UBV and B-V light curves made with these observations cover phases nearly a half of one period. There is no appreciable light variation in V but in other two passbands a gradual decrease of the brightness is clearly noticed. The loss of light in B resulted in a reddening in $\Delta(B-V)$ by + 0.06 at phases between 0.4~0.5 as compared with that of at phase ~0.1. This intrinsic reddening arouses a question why at the orbital phase of the transit of a hot star in front of a cool M supergiant the heating of the facing hemisphere of M supergiant by the strong radiation from the B stat is absent. With regard to this unusual situation we propose a hypothesis that a large amount of gas stream of low temperature ejected from the surface of M supergiant component towards the B star dominates the brightness of B star and the reflection effect.

• Structural Engineering and Mechanics
• /
• v.2 no.3
• /
• pp.257-267
• /
• 1994

Motions of an unsymmetric rigid body on a rigid floor subjected to earthquake excitations with special attention to coefficient of friction are investigated. Motions of a body in a plane are classified (Ishiyama 1980) into six types, i.e. (1) rest, (2) slide, (3) rotation, (4) slide rotation, (5) translation jump, (6) rotation jump. Based upon the theoretical and experimental research work special attention is paid to the sliding of a body. The equations of motions and the behavior of coefficient of friction in the time of floor excitation are studied. One of the features of this investigation is the introduction and estimation of the "time dependent" coefficient of friction. It has been established that the constant kinetic coefficient of friction $${\mu}(kin){\sim_\sim}0.8{\mu}(stat)$$ does not give the appropriate results. The method for the estimation of the friction coefficient variation during the time is given.

• Molecular & Cellular Toxicology
• /
• v.5 no.2
• /
• pp.147-152
• /
• 2009

This study was conducted to evaluate the inflammatory mechanisms of LPS-stimulated BV-2 microglial cells. The inflammation mechanism was evaluated in BV-2 cells with or without LPS treated using the Affymetrix microarray analysis system. The microarray analysis revealed that B cell receptor signaling pathway, cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, MAPK signaling pathway, Neuro-active ligand-receptor interaction, TLR signaling path-way, and T cell receptor signaling pathway-related genes were up-regulated in LPS stimulated BV-2 cells. Selected genes were validated using real time RTPCR. These results can help an effective therapeutic approach to alleviating the progression of neuro-in-flammatory diseases.

• Journal of Microbiology and Biotechnology
• /
• v.9 no.5
• /
• pp.554-561
• /
• 1999

A novel estimation method was investigated for determining the concentrations of residual biomass, poly-3-hydroxybutyrate (PHB), and main nutrients including carbon and nitrogen sources, phosphate, and mineral ions from the supplied amount of ammonia solution used for a pH-control solution and nitrogen source in a PHB fermentation. The estimation equations for a batch culture and a fed-batch culture were derived from the relationship between the growth rate of residual biomass and the feed rate of the pH-control solution, and then were applied to the batch culture and the fed-batch cultures of Alcaligenes latus. This method was successfully applied to estimate the concentrations of residual biomass, PHB, and nutrients.

• Journal of Microbiology and Biotechnology
• /
• v.9 no.6
• /
• pp.722-725
• /
• 1999

Filamentation-suppressed recombinant Escherichia coli strain harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes and the E. coli ftsZ gene was constructed and cultivated for the production of poly(3-hydroxybutyrate) [P(3HB)] with high concentration and high content. By the pH-stat fed-batch culture of this recombinant E. coli strain XL1-Blue(pJC5), the final cell concentration and P(3HB) concentration obtained in 44.25h were 172.2g cell dry weight/l and 141.9g P(3HB)/l, respectively, resulting in productivity of 3.21g P(3HB)/l-h. More importantly, the P(3HB) content obtained was 82.4 wt %, which was significantly higher than that obtained with the recombinant E. coli harboring only the PHA biosynthesis genes.

• Journal of Families and Better Life
• /
• v.28 no.6
• /
• pp.157-167
• /
• 2010

This study examined the relationships among children's attachment to the mother, trait anger, anger expression style, and emotional-behavioral problems in children. The subjects were 384 5th and 6th graders at elementary schools in J and G cities of Jeonbuk-Do. The instruments were Revised Inventory of Parent and Peer Attachment (IPPA-R), Stat-Trait Anger Expression Inventory - Korean version (STAXI-K), and Strength and Difficulty Questionnaire (SDQ). The major results are as follows; The level of children's attachment to the mother was negatively related to anger and emotional-behavioral problems, and the level of trait anger was positively related to emotional-behavioral problems. Children who have high anger expression and anger suppression tended to show more externalized behavior problems. Finally, the regression analysis showed that children's attachment to the mother and anger are revealed as significant factors in emotional-behavioral problems.

• KSBB Journal
• /
• v.15 no.5
• /
• pp.423-427
• /
• 2000

Intense research on biosensors has been performed in a number of different institution over the past 15 years, but relatively few commercial products have resultingly, the blood glucose sensor is a good example of a product which penetrated the market. However recently, the development of electrochemical and optical technologies has accelerated the turnover of the research as is illustrated by a rapid increase in the number of point-of-care diagnostic systems and analytical devices. Examples of such biosensors used in the fields of medical diagnostics, bioprocess control, and environmental monitoring are described, and summarized in an introduction to their characteristics, structures, and functions, given.

• Journal of Microbiology and Biotechnology
• /
• v.10 no.5
• /
• pp.628-631
• /
• 2000

The timing of poly(3-Hydroxybutyrate) (PHB) biosynthesis was controlled by varying the agitation speed of a stirred tank fermentor during the pH-stat fed-batch culture of recombinant Escherichia coli strain GCSC 6576 harboring pSYL107. Using a concentrated whey solution containing ca. 200 g/l lactose as the nutrient feed, the PHB content was only 57% after 35h due to volumetric limitation of the fermentor. However, by limiting the oxygen by maintaining the agitation speed at 300 rpm, the final PHB content increased to 70% after 70h with a cell concentration of 15 g/l. When the agitation speed was increased up to 500 rpm, a cell concentration of 31 g/l with 80% PHB was obtained after 52h. A further increase in the maximum agitation speed increased the cell concentration, PHB concentration, and PHB productivity, however, the PHB content decreased to 56-58%.

• Bulletin of the Korean Chemical Society
• /
• v.13 no.4
• /
• pp.381-384
• /
• 1992

The hydrolytic susceptibility of large unilamellar vesicle (LUV) toward cabbage phospholipase D (PLD) was studied. The activity of PLD was determined by pH stat titration method. Using phosphatidylcholine LUV as substrate a pH optimum of 6.96 was observed. For maximal activity the optimal temperature of $31^{\circ}C$ and 10 mM of Ca2+ were required. The apparent Km value estimated was 2.5 mM. The hydrolytic activity of PLD toward PC LUV was somewhat high despite the absence of activator in assay system and this high susceptibility of PC LUV may be attributed to the structural properties of LUV. The effect of amphiphatic substances such as dicetyl phosphate and phosphatidic acid on the enzyme activity were also examined in mixed LUVs.

• Genomics & Informatics
• /
• v.17 no.1
• /
• pp.8.1-8.10
• /
• 2019

Alveolar type II cells constitute a small fraction of the total lung cell mass. However, they play an important role in many cellular processes including trans-differentiation into type I cells as well as repair of lung injury in response to toxic chemicals and respiratory pathogens. Transcription factors are the regulatory proteins dynamically modulating DNA structure and gene expression. Transcription factor profiling in microarray datasets revealed that several members of AP1, ATF, $NF-{\kappa}B$, and C/EBP families involved in diverse responses were expressed in mouse lung type II cells. A transcriptional factor signature consisting of Cebpa, Srebf1, Stat3, Klf5, and Elf3 was identified in lung type II cells, Sox9+ pluripotent lung stem cells as well as in mouse lung development. Identification of the transcription factor profile in mouse lung type II cells will serve as a useful resource and facilitate the integrated analysis of signal transduction pathways and specific gene targets in a variety of physiological conditions.