• Title/Summary/Keyword: Staphylococcus sp

Search Result 208, Processing Time 0.024 seconds

미이용(未利用) 식물자원(植物資源)의 연구(硏究)(I) -식용(食用) 야초(野草)의 성분(成分)에 관하여- (Studies on Unutilized Plant Resources(I) -Components of Wild Edible Plants-)

  • 김태희
    • 생약학회지
    • /
    • 제6권1호
    • /
    • pp.23-27
    • /
    • 1975
  • To evaluate nutritional values and to detect pharmacologically active and antibacterial components of wild edible plants in Korea, 20 species were examined. 1) The essential free amino acid compositions of these plants were determined by TLC. The plants contained threonine, leucine, valine and methionine. 2) In antibacterial tests of 17 species the plants Hemerocallis sp. and Plantago asiatica showed an antibacterial activity against Staphylococcus, Bacillus, Eschericia, and Salmonella species. 4) Rutin. quercitrin, $k\ddot{a}mpferol$, chlorogenic acid and caffeic acid were also identified.

  • PDF

12,13-Dihydroxyfumitremorgin C, Fumitremorgin C, and Brevianamide F, Antibacterial Diketopiperazine Alkaloids from the Marine-Derived Fungus Pseudallescheria sp.

  • Zhang, Dahai;Noviendri, Dedi;Nursid, Muhammad;Yang, Xiu-Dong;Son, Byeng-Wha
    • Natural Product Sciences
    • /
    • 제13권3호
    • /
    • pp.251-254
    • /
    • 2007
  • Dioxopiperazine alkaloids, 12R,13S-dihydroxyfumitremorgin C (1), fumitremorgin C (2), and brevianamide F (3), were isolated from the marine-derived fungus Pseudallescheria, and the absolute stereostructures of compounds 1 - 3 were elucidated on the basis of chemical and physicochemical evidence. Compounds 1 - 3 showed an antibacterial activity against Staphylococcus aureus, methicillin-resistant S. aureus, and multidrug-resistant S. aureus. The MIC (minimum inhibitory concentration) values of compounds 1 - 3 were 125 ${\mu}g/mL$ for all strains.

소 자궁내막염에 대한 미생물학적 분포 (Microbiological studies on endometritis in cattle and dairy cows)

  • 강신석;최필규;한성태;이용희;유정희
    • 한국동물위생학회지
    • /
    • 제21권1호
    • /
    • pp.21-27
    • /
    • 1998
  • In order to investigate reproductive disorder in dairy cows, 312 uterine from abattroirs and 56 cows from dairy farms were examined. The ovary and uterine were collected from abattroirs and rectal palpation was carried out in the case of reproductive disorder at dairy farm. Microbiological examination was also carried out from the collected samples. 1. Out of 312 ovaria from slaughter-house, cystic ovary and endometritis were classified as 51(16%) and 134(43%), respectively. And of 56 dairy cow from farms, 43(77%) were identifies as endometritis. 2. The main cause of reproductive disorder was classified as hormonal imbalances, endometritis and cystic ovary, singly or in combination. 3. The main causative agents of endometritis was encountered as E coli, Staphylococcus aureus, Bacillus sp and Corynebacterium sp. 4. The highest susceptible antibiotics to the isolates was cephalosporin.

  • PDF

The Synergistic Antibacterial Activity of 1-Acetyl-$\beta$-Carboline and $\beta$-Lactams Against Methicillin-Resistant Staphylococcus aureus (MRSA)

  • Shin, Hee-Jae;Lee, Hyi-Seung;Lee, Dae-Sung
    • Journal of Microbiology and Biotechnology
    • /
    • 제20권3호
    • /
    • pp.501-505
    • /
    • 2010
  • 1-Acetyl-$\beta$-carboline was isolated as an anti-MRSA agent from the fermentation broth of a marine actinomycete isolated from marine sediment. The producing strain was identified to be Streptomyces sp. by phylogenetic analysis of the 16S rRNA gene sequence. The anti-MRSA agent was isolated by bioactivity-guided fractionation of the culture extract by solvent partitioning, ODS open flash chromatography, and purification with a reversed-phase HPLC. Its structure was elucidated by extensive 2D NMR and mass spectral analyses. Combination of 1-acetyl-$\beta$-carboline with ampicillin exhibited synergistic antibacterial activity against MRSA.

Graphiumins I and J, New Thiodiketopiperazines from the Marine-derived Fungus Graphium sp. OPMF00224

  • Fukuda, Takashi;Nagai, Kenichiro;Kurihara, Yuko;Kanamoto, Akihiko;Tomoda, Hiroshi
    • Natural Product Sciences
    • /
    • 제21권4호
    • /
    • pp.255-260
    • /
    • 2015
  • Two new thiodiketopiperazines (TDKPs), designated graphiumins I (1) and J (2), were isolated from the culture broth of the marine-derived fungus Graphium sp. OPMF00224 by solvent extraction, silica gel column chromatography, and HPLC. Their absolute structures were elucidated by spectroscopic analyses (1D and 2D NMR data, ROESY correlations, and CD data) and chemical methods. They were found to be structurally rare TDKPs with a phenylalanine-derived indolin substructure. Compounds 1 and 2 inhibited yellow pigment production by methicillin-resistant Staphylococcus aureus (MRSA) with $IC_{50}$ values of 63.5 and $76.5{\mu}g/ml$, respectively, without inhibiting its growth, even at $250{\mu}g/ml$.

게 가공폐기물을 이용한 식품보존료의 개발에 관한 연구 (A Development of Food Preservative with the Waste of Crab Processing)

  • 장동석;조학내;구효영;최위경
    • 한국수산과학회지
    • /
    • 제22권2호
    • /
    • pp.70-78
    • /
    • 1989
  • 게 껍질 속에 함유된 chitin으로 부터 높은 항균력을 나타내는 chitosan의 제조 방법과 특성을 구명하였으며, 이 chitosan을 이용하여 식품의 변질에 관련된 미생물을 중심으로 항균력을 조사하여 천연 식품 보존료로 이용 가능성을 검토하였다. 1. 상법에 따라 조제한 분자량 2,800,000의 고분자 chitosan은 2,000ppm의 농도로 대장균의 증식을 억제시킬 수 있는 등, 항균력을 보유하고 있기는 하였으나 미약하였다. 2. Deacetylation 처리 시 $50\%$ 수산화나트륨 용액에서 8시간 boiling시켜 얻은 분자량 35,000의 저분자 chitosan은 75ppm으로 대장균의 중식을 억제시킬 수 있어서 고분자 chitosan에 비해 20배 가량 항균력이 증대되었다. 3. 세균에 대한 chitosan의 항균력에 있어서 Bacillus sp. 나 구균류 등의 Gram 양성균은 50ppm 첨가로 균의 증식이 억제되었으나, Gram 음성균에 대해서는 균종에 따라 차이가 커 Pseudomonas sp. 와 Vibrio sp. 는 100ppm으로, Salmonella는 2,000ppm으로 증식을 억제시킬 수 있었다. 4. 진균류에 있어서 Saccharomyces sp.는 100ppm으로 억제되었으나, Hansenula sp.는 5,000ppm에서도 증식이 일어나 효모류는 균종간에 차이가 켰으며, 곰팡이류에 대해서는 별 효과가 없는 것으로 나타났다. 5. 숙성시킨 물김치에 chitosan을 첨가하여 저장수명을 연장시킬 수 있었는데, chitosan $0.2\%$ 첨가후 $5^{\circ}C$에 저장함으로서 무첨가구에 비해 10일 가량 품질을 안정하게 유지시킬 수 있었다. 따라서 이 chitosan은 천연 식품 보존료로 이용 가능하다고 하겠다.

  • PDF

오미자 효소가수분해물에 의한 장내 세균총 개선 및 장관 염증 억제 효과의 효소학적 평가 (Enzymological Evaluation of Bowel Inflammation Inhibitory Activity and Intestinal Microbial Flora Improvement by Enzymatic Hydrolysate of Schizandrae Fructus)

  • 류일환;권태오;이강수;윤용갑
    • 생약학회지
    • /
    • 제38권4호
    • /
    • pp.363-371
    • /
    • 2007
  • Schizandrae Fruits has been used as a traditional Oriental medicine for treatment of many stress-induced diseases. In the present study, we investigated inhibitory activity of enzymatic hydrolysate of Schizandrae fructus (SC-EX) in growth of tested intestinal microorganism and activity of bowel inflammation related enzyme. SC-EX was added to the proteose peptone-yeast extract-fildes (PYF) media to investigation the effect on the growth of type culture of intestinal microorganism. The growth of lactic acid bacteria such as Bifidobacterium species and Lactobacillus species was accelerated by more than 3% concentration of SC-EX. But, growth of harmfulness bacteria such as E.coli, Clostridium sp. Staphylococcus sp. Streptococcus sp. was inhibited by more than 3% concentration of SC-EX. Also, SC-EX was exhibited a concentration-dependent inhibitory activity of the bowel inflammation related enzymes. The SC-EX was showed 76% and 92% inhibitory activity of 5-lipoxygenase and cyclooygenase at 5% additional concentration respectively. Our results indicated that SC-EX may possess improvement effect on the intestinal flora and Anti-inflammatory effect on the bowel.

메치실린 내성 포도상구균에 유효한 항생물질을 생산하는 Streptomyces sp. YSK-681의 분리 및 수리 동정 (Isolation and Numerical Identification of Antibiotic-Producing streptomyces sp. for Methicillin Resistant Strphylococcus aureus)

  • 김중배;이동희;신운섭;고춘명
    • 한국식품영양학회지
    • /
    • 제11권3호
    • /
    • pp.340-346
    • /
    • 1998
  • 항생제에 대한 내성균의 출현은 매년 급격하게 증가되고 있으며 이에 대한 약제 개발이 요구되고 있다. 특히 메치실린내성 황색 포도상구균(MRSA)은 여러 항생물질에 대하여 다제내성을 나타내므로 감염증치료에 어려운 문제점으로 남아 있다. 이러한 감염증치료에 사용할 목적으로 임상 가검물에서 분리한 MRSA를 시험균으로 사용하여 항균 활성이 강한 방선균인 YSK-681을 선별하였다. 균주 동정을 위하여 선별된 YSK-681을 형태적, 배양학적, 생리적, 화학적 분석을 하였으며 41개의 단위 항목을 조사하여 TAXON program으로 수리 동정한 결과 주군집 29개에 속하는 Streptomyces lydicus로 동정하였다.

  • PDF

미세유체 칩 내에서 유전영동 집게(Dielectrophoretic Tweezers) 를 이용한 단백질A와 면역 글로불린 G의 결합에 관한 연구 (Investigation of the Binding Force between Protein A and Immunoglobulin G Using Dielectrophoretic(DEP) Tweezers Inside a Microfluidic Chip)

  • 곽태준;이재우;윤대성;이상우
    • 대한의용생체공학회:의공학회지
    • /
    • 제34권3호
    • /
    • pp.123-128
    • /
    • 2013
  • The 'Dielectrophoretic Tweezers(DEP Tweezers)' can be used as a facile, economical toolkit for quantitative measurement of chemical and biological binding forces related to many biological interactions within a microfluidic device. Our experimental setup can probe the interaction between a single receptor molecule and its specific ligand. Immunoglobulin G(IgG) functionalized on polystyrene microspheres has been used to detect individual surface linked Staphylococcus protein A(SpA) molecules and to characterize the strength of the noncovalent IgG-SpA bond. It was measured and compared with the existing measurements. Measured single binding force of between Goat, Rabbit IgG and SpA were $17{\pm}7pN$, $74{\pm}16pN$. This work can be used to investigate several different ligand-receptor interactions and antigen-antibody interactions.

Screening and Characterization of Pro biotic Lactic Acid Bacteria Isolated from Korean Fermented Foods

  • Lim, Sung-Mee;Im, Dong-Soon
    • Journal of Microbiology and Biotechnology
    • /
    • 제19권2호
    • /
    • pp.178-186
    • /
    • 2009
  • To examine their potential as probiotics, acid and bile tolerance, antibiotics resistance, adhesion capacity to Caco-2 and HT-29, and antibacterial activity, of LAB isolated from Korean fermented foods such. as dongchimi, kimchi, Meju, and doenjang were assayed against foodborne pathogenic bacteria. DC 55, DC 136, DC 222, KC 21, KC 24, KC 34, KC 43, KC 117, MJ 54, MJ 301, SP 33, and SP 170 strains were resistant to acid and bile conditions. In particular, DC 55, DC 136, KC 24, KC 43, and MJ 301 strains were highly resistant to higher than 20 ${\mu}g/ml$ concentrations of vancomycin, streptomycin sulfate, or amoxicillin, whereas, DC 222, KC 21, KC 34, KC 117, MJ 54, and SP 33 strains were susceptible to lower than 2 ${\mu}g/ml$ concentrations of those antibiotics. The adhesion to HT-29 and Caco-2 cells varied with the strains tested in a strain-dependent manner. The highest level of adhesion was observed with DC 55, KC 21, KC 24, and MJ 301 strains, having higher than 50% of adhesion to HT-29 or Caco-2 cells. In addition, Staphylococcus aureus was the most sensitive to KC 21, showing an inhibition of about 70%, and the antibacterial activity of KC 21 against S. aureus resulted most likely from both organic acids and bacteriocin. Based on its phenotypic characteristics and utilization of various sugars, the KC 21 strain was identified as Lactobacillus plantarum.