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Esterification Reaction of Animal Fat for Bio-diesel Production (바이오디젤 생산을 위한 동물성 오일의 에스테르화 반응)

  • Kim, Sung-Min;Kim, Deog-Keun;Lee, Jin-Suk;Park, Soon-Chul;Rhee, Young-Woo
    • Clean Technology
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    • v.18 no.1
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    • pp.102-110
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    • 2012
  • In this study, the production of bio-diesel from animal oil by esterification and trans-esterification was investigated. There were three different extraction methods for oil extraction from raw animal fat. Heterogeneous catalysts such as Amberlyst-15 and Amberlyst BD-20 and a homogeneous catalyst such as sulfuric acid were used for esterification. Among three catalysts, the removal efficiency of Free Fatty Acid (FFA) was the highest in sulfuric acid. Response surface method was carried out to find the optimal esterification condition of sulfuric acid and methanol. After the esterification under the optimal condition, this animal fat was used for the trans-esterification. Animal oil used for trans-esterification was below 1% of FFA content and 0.09% of water content. The catalysts for trans-esterification were KOH, NaOH and $NaOCH_3$. To investigate the effects of catalyst type and amount on trans-esterification, The amount of catalyst were changed with 0.3, 0.6 and 0.9 wt%. The molar ratio of methanol/oil was changed with 4, 6, 9 and 12. The amount of catalyst was fixed to 0.8 wt%. The KOH catalyst showed the highest FAME conversion for trans- esterification, and the optimal methanol/oil weight ratio was 6. In the experiments of various catalysts and methanol molar ratios, the highest content of FAME is 96%. However, this FAME content was below Korean bio-diesel standard which is 96.5% of FAME content. After distillation, FAME content increased to 98%.

Effect of Artificial Light Sources on the Growth of Apple Rootstock M.9 Seedling (인공광원이 사과 대목 M.9 묘 생육에 미치는 영향)

  • So, Eun Hee;An, Jin Hee;Kim, Jae Kyung;Yoon, Yeo Joong;Park, So Jeong;Na, Jong-Kuk;Choi, Ki Yong
    • Journal of Bio-Environment Control
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    • v.27 no.4
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    • pp.341-348
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    • 2018
  • To investigate the effect of light sources on the growth and photosynthesis of the dwarf apple rootstock M.9 for the production of standard seedlings, the plants were cultivated in a controlled environment for 6 weeks. The sources of light are six treatments [Red (R), Blue (B), White (W), RBUV (R7B3 containing UV-A), RBW (R3B1W1), SMF (high pressure sodium + metal halide + fluorescent lamp) under $154{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Growth characteristics of apple seedlings varied depending on artificial light source at 3 weeks and 6 weeks. The plant height of apple seedling was high in the R, RBUV, RBW, and SMF light sources at 3 weeks, and in the R light at 6 weeks. There was no significant difference on stem diameter among the treatments at 3 weeks, but showed high in RBUV and RBW light at 6 weeks. Leaf number was the highest in RBUV light at 3 and 6 weeks. The chlorophyll content (SPAD value) was high in the B and RBUV light at 3 weeks, but it was not significant at 6 weeks. The growth rate to height of the R light (1.12mm/day) was the highest among the treatments, followed by RBUV, RBW, SMF, W and then B. Leaf area was the highest in RBUV and RBW lowest in B. Specific leaf area was high in W and fresh and dry weight were high in RBUV. The photosynthetic rate at 6 weeks was highest in the B and lowest in the R. Stomatal conductivity and transpiration rate were higher in the B and W compared to the other light sources. Therefore, we are considered that light sources for growth of dwarf apple rootstock M.9 seedlings are suitable the R, RBUV, and RBW light sources with a high mixing ratio of Red and Red +Blue.

Effect of Nitrogen fertilizers on Soil pH, EC, NO3-N and Lettuce(Lactuca Sativa. L.) Growth (질소비종이 토양의 pH, EC, NO3-N 함량 및 상추 생육에 미치는 영향)

  • Lee, Gyeong-Ja;Kang, Bo-Koo;Kim, Hyun-Ju;Park, Seong-Kyu;Min, Kyeong-Beom
    • Korean Journal of Soil Science and Fertilizer
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    • v.34 no.2
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    • pp.122-128
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    • 2001
  • In order to find out the effect of nitrogen fertilizers on soil pH, EC, $NO_3-N$ and lettuce growth, this study was conducted by pot experiment in plastic film house condition. The square-pot which was $42{\times}54.5{\times}22cm$ in length, width and height, respectively, was filled with two kinds of soils in different soil EC as $0.20dS\;m^{-1}$ and $1.13dS\;m^{-1}$. Seven kinds of nitrogen fertilizers (urea, potassium nitrate, calcium nitrate, ammonium nitrate, ammonium sulphate, complex fertilizer A(11-10-10) and complex fertilizer B(12-12-12)) were treated in same standard rate of nitrogen for lettuce, transplanted the six lettuce seedlings of 10 days grown per pot, and have been grown for 38~44 days with three times harvesting. Soil pH was increased with the potassium and calcium nitrate treatments and decreased with ammonium nitrate, ammonium sulphate, complex fertilzer A and B, and the pH of urea treatments was kept the same value as the pH of before experiment. The growing status of lettuce seedling were surveyed during the early period after transplanting and withering of seedling was occured in all treatments. The withering rates were 10% in soil of EC $0.20dS\;m^{-1}$ and 44% and 42% in complex fertilizer and ammonium sulphate treaments, respectively, in soil of EC $1.13dS\;m^{-1}$. $NO{_3}^-$ contents of lettuce were about $1,000{\sim}2,000mg\;kg^{-1}$ based on fresh weight and these contents were considered to be lower to compare the $NO{_3}^-$ level of EU countries.

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Recommendation Rate of Slow Release Nitrogen Fertilizer Application for Early Dry Seeding Culture of Rice in Honam Plain Area (호남평야지에서 벼 건답직파 조기파종재배에 알맞은 완효성 질소비료 시비량)

  • Kim, Sang-Su;Choi, Weon-Yeong;Back, Nam-Hyun;Choi, Min-Gyu;Park, Hong-Kyu;Nam, Jeong-Kweon
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.5
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    • pp.292-297
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    • 2006
  • A research was carried out to investigate the release pattern of slow release nitrogen fertilizer compound(SRNC) that is latex coated urea(LCU) and to recommend the application rate of the fertilizer at dry seeding rice culture in Honam plain area. The experiment was conducted at experimental field(Jeonbug series, fine silty, mixed, nonacid, mesic family of Fluvaquentic Endoaquepts) of National Honam Agricultural Research Institute. A medium late maturing rice variety (Dongjinbyeo) was seeded at the rate of $60kg\;ha^{-1}$ by drill seeder on April 1, 1997 and March 30, 1998. Fertilizer application rate was $160-90-110kg\;ha^{-1}$ for $N-P_2O_5-K_2O$. Soil ammonium nitrate($NH_4-N$) contents remained higher in all SRNC plots than conventional ones and higher with the increase of SRNC application rate until panicle formation stage, but the contents was higher in the conventional than NRNC plots at the heading stage. The plant hight was taller in SRNC than conventional plots until maximum tiller stage, but became similar in both conventional and SRNC plots at heading stage. The culm and panicle number was greater in SRNC than conventional plots throughout all growth stage, but the effective tiller rate was higher in conventional ones. Nitrogen efficiency was higher in the SRNC than conventional plots, but the efficiency was decreasing with the higher SRNC level. The spikelet number per unit area was greater in SRNC than conventional plots, and increased with higher SRNC level. The more spikelet number with higher NRNC level resulted in lower 1,000-grain weight. The rice yield in conventional plot was similar with only 60% SRNC level, but lower than 80% and 100% SRNC levels. However, slight lodging was observed in 100% SRNC level. In conclusions, we recommend NRNC application level as 80% of standard nitrogen application for early dry seeding culture of rice Honam plain area.

Effect of Nitrate Concentration in Culture Solution on the Growth and the Uptake of Inorganic Elements of Tomato Plants(Lycopersicum esculentum Mill) (배양액(培養液)의 질산태질소농도가 토마토(Lycopersicum esculentum Mill)의 생육 및 무기원소(無機元素) 흡수에 미치는 영향)

  • Lee, Kwang-Seek;Yu, Jing-Quan;Matsui, Yoshihisa
    • Korean Journal of Soil Science and Fertilizer
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    • v.30 no.1
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    • pp.29-34
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    • 1997
  • This experiment was carried out to investigate the effects of nitrate concentration in culture solution on the growth and the uptake of inorganic elements in Tomato plant in the greenhouse. Tomato plants(cv. TVR-2) were grown with nitrate concentrations 8, 16, 24, 32cmol/l, based on Japan ENSI standard solution. Dry weights of lamina and petiole increased with the nitrate concentration. However, the dry weight of fruit was the highest in the treatment of nitrate concentration of 16cmol/l. The proportion of dry weights of vegitative organ to reproductive organ was the lowest in the treatments of nitrate concentrations of 16cmol/l and it increased with the nitrate concentration. The fruit yield was the highest at the treatment of nitrate concentration of 16cmol/l. With the increase of nitrate level the concentrations of N, $NO_3-N$, Ca and Na increased in lamina and petioles. The concentrations of K, P, S and Cl tended to decline in the nitrate concentration of 16 and 32cmol/l. These results indicate that optimum nitrate concentrations in a tomato grown by hydroponics change with growth stage, and the optimum concentrations for vegitative and reproductive stage were 8 and 16cmol/l, respectively. It also was proved that the nitrate concentrations in the culture solution affected antagonistically the uptake of inorganic anion in tomato : In low nitrate level $Cl^-$ uptake was affected much, while $SO_4{^{2-}}$ and $H_2PO_4{^-}$ uptake were affected in high nitrate level.

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A Study of Adjustment for Beginning & Ending Points of Climbing Lanes (오르막차로 시.종점 위치의 보정에 관한 연구)

  • 김상윤;오흥운
    • Journal of Korean Society of Transportation
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    • v.24 no.5 s.91
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    • pp.35-44
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    • 2006
  • Acceleration and deceleration curves have been used for design purposes worldwide. The curve in design level has been regarded as an single deterministic curve to be used for design of climb lanes. It should be noted that the curve was originally made using ideal driving truck and that the curve is applied during design based on the assumption of no difference between ideal and real driving conditions. However. observations show that aged vehicles and lazy behavioring drivers nay make lower performance of vehicles than the ideal performance. The present paper provides the results of truck speeds at climbing lanes then probabilistic variation of acceleration and deceleration corves. For these purposes. a study about identification of vehicle makers, and weights for trucks at freeway toll gates and then observation of vehicle-following speed were performed. The 85%ile results obtained were compared with the deterministic performance curves of 180, 200, and 220 Ib/hp. It was identified that the performance of 85%ile results obtained from vehicle-following-speed observations were lower than one from deterministic performance curves. From these results, it may be concluded that at the beginning Point of climbing lanes additional $16.19{\sim}67.94m$ is necessary and that at the end point of climbing lanes $53.12{\sim}103.24m$ of extension is necessary.

Quality changes in Agaricus bisporus varieties due to period and temperature during their storage (양송이버섯 품종별 저장기간 및 온도에 따른 품질변화)

  • Oh, Youn-Lee;Jang, Kab-Yeul;Jhune, Chang-Sung;Kong, Won-Sik;Yoo, Young-Bok;Shin, Pyung-Gyun;Seo, Jang-Sun
    • Journal of Mushroom
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    • v.11 no.3
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    • pp.137-144
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    • 2013
  • This study was carried out to investigate storage stability of harvested mushroom in developed varieties of button mushroom through identification of quality change during 35 days. The mushroom harvested up to $2^{nd}$ flush mushroom was stored on different storage temperature(4, 7, $10^{\circ}C$) and weight, length, thickness, color of pileus and stipe in fruit body was tested every 7 days. The morphological trait and color of stipe in fruit body were influenced by harvesting period and storage temperature. On the morphological traits of fruit body, mushroom harvested at $2^{nd}$ flush showed smaller difference than those at $1^{st}$ flush and mushroom stored at $4^{\circ}C$ indicated more difference than ones at the other temperature. Mostly color of pileus in brown button mushroom had lower ${\Delta}E$ (color difference) than white mushroom during storage period. The color of pileus of fruit body in white mushroom had low ${\Delta}E$ at $4^{\circ}C$ whereas that of brown mushroom was low at $10^{\circ}C$. The safe period of storage based on the L value is 7 days under a specified environmental condition. 'Seolgang' was stored for 14 days as good quality of mushroom, 'Saedo' had best quality after harvesting and 'Seojeong' was most stable during storage change in white mushroom varieties.

A Nutrition Intakes Survey of Pregnant Women in a Urban Area -Application of Convenient Method for the Study of Nutritionial Status- (일개 도시지역 임신부의 영양섭취에 관한 조사연구 -간이식 영양조사법 이용-)

  • Kim, In-Sook
    • Journal of Preventive Medicine and Public Health
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    • v.16 no.1
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    • pp.99-104
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    • 1983
  • Aiming at the total 200 pregnant women including 150 ones registered with the Health Center of Chung Ku District and 50 ones registered with the Health Center of Mapo District, we have conducted a research study of the socio-medical characteristics, maternal child health, and the status of nutritional intake which has utilized the application of convenient method for the study of nutritional status, during the period of April 20 to May 25, 1983. And we have obtained the following conclusions: 1. Regarding age distribution, the pregnant women aged from 26 to 30 were most numerous, which was 54.0%. The percentage of women who experienced the first pregnancy was 12.0% and the percentage of those who experienced the second pregnancy was 37.0%, which was the highest. Regarding the weeks of pregnancy of the pregnant women, the first trimesterr was 11.5%, the second trimester 30.0%, and the third trimester 58.6%. 2. Regarding academic achievements, the pregnant women who graduated from middle schools reached 43.5%, which was the highest percentage. Regarding economic status, the pregnant women who owned their own houses were only 21.0%. And the pregnant women whose monthly income was from 300,000 won to 400,000 won were 40%, which was the most numerous. 5. The women above 15 years old who experienced the first menstruation were 84.0%. And those who experienced abortion were totally 54.4%. and 35.5% among those women experienced artificial abortion. 4. 70.5% of the pregnant women said that their health condition was excellent, 24.5% felt subjective complaints, and 5.0% specially received medical consultation for their diseases. 5. 82.0% received prenatal care, but 60.5% regularly received prenatal care. 68.0% received the education for nutrition and only 19.5% regularly received the education for nutrition. 6. Regarding the family composition, the families consisting of two generations were 47.0%, which was the most numerous. 97.5% of the preparation for meals was conducted by housewives. They said that they did not lack time for meal preparation. 7. 94.9% of the pregnant women said that they had eaten as in ordinary times during their pregnancy. 25.5% said that there were tabooed foods. Tabooed foods are chiefly pork, chicken, milk, and eggs. 68.0% don't drink milk during pregnancy, 32.5% take the intake of vitamins, and 20.5% take iron supplement. 8. The average amounts of the intake of protein, fat, and carbohydrate of a pregnant women are 49.3gm, 29.4gm, and 205.1gm respectively, which showed the phenomenon in which the amount of the intake of nutrition increased as the weeks of pregnancy increased. The average amount of the intake of salt was 14.2gm. 9. Regarding the hemoglobin value of all pregnant women, those whose hemoglobin value was less than 11.0gm were 66.5%, those whose hemoglobin value was 11 to 12 gm were 16.5%. and those whose hemoglobin value was above 12gm were 17.0%. The pregnant women whose hemoglobin value was less than 11.0gm in the first trimester of pregnancy, in the second trimester, and in the trimester were 81.8%, 62.8%, and 64.9% respectively. This shows that the phenomenon of anemia increased as the weeks of pregnancy increased 10. Regarding physical development of a pregnant woman during prenatal period, the height and weight were $156.7cm{\pm}14.1$ and $51.1kg{\pm}58$ respectively. When the standard increase of a prenatal weigt gain is set as 100%, the women over the range of 100% were 28.0% and the women under the range of 80% were 37.0%.

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Effect of Topophysis and Uniting Method of Rootstock and Scion on Rooting and Subsequent Growth of Stenting-propagated (Cutting-grafted) Roses (접수의 채취부위 및 접수와 대목의 고정법에 따른 장미 접삽묘의 생육 특성)

  • Park, Yoo-Gyeong;Jeong, Byoung-Ryong
    • Horticultural Science & Technology
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    • v.28 no.3
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    • pp.456-461
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    • 2010
  • A study was conducted to investigate the effect of topophysis, and uniting method of rootstock and scion on rooting and subsequent growth of stenting-propagated cut rose ($Rosa$ $hybrida$ Hort.) in an effort to develop an efficient stenting propagation method for domestic rose cultivars. Four cultivars used in this study were two standard type cultivars 'Sweet Yellow' and 'Hanmaum', and two spray type cultivars 'Chelsi' and 'May'. Scions were grafted on cuttings of a rootstock $Rosa$ $indica$ 'Major'. The stenting-propagated scion-rootstock unions were planted in rockwool cubes ($50{\times}50{\times}50mm$, Delta, Grodan, Denmark) and were placed in a graft-take chamber for five days before being placed on misted greenhouse beds. The rootstock was removed of all leaves and nodes. Both the base of scions and top of stocks were simultaneously cut at a $45^{\circ}$ angle for grafting. Scions were prepared as single node cuttings, each with a five-leaflet leaf. Three positions of topophysis used were 7-9th (top), 4-6th (middle), and 1st-3rd (bottom) nodes from the stem base. Four uniting materials used were tube, tube + parafilm wrap, tube + clothespin, and clothespin. Rooting and growth were affected by the topophysis and cultivar. The best topophysis for rooting was 7-9th (top) nodes in all cultivars. Topophysis affected percent rooting, and number of roots, length of the longest root, and but not weight, shoot length and graft-take. Rooting and growth were affected by the uniting method and cultivar. Tube uniting method generally showed higher percentage graft-take, percent rooting, and number of roots than other methods. However, rootstock and scion union was not complete in this treatment. On the whole, the greatest rooting and subsequent growth of stenting-propagated plants were found in the tube + clothespin method. Except 'Sweet Yellow', rooting and growth were not adequate in the clothespin method. The results suggested that a tube + clothespin method was the most effective, and this method may be used as a substitute to save labor compared to a tube + parafilm wrap method which is currently being used in commercial nurseries.

A Study of Fish Community on Up and Downstream of Hwabuk Dam Under Construction in the Upper Wie Stream. (위천 상류에 건설 중인 화북댐 상 하류 어류군집에 관한 연구)

  • Seo, Jin-Won;Kim, Hee-Sung
    • Korean Journal of Ecology and Environment
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    • v.42 no.2
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    • pp.260-269
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    • 2009
  • Hwabuk Dam has been under construction to reduce flood damage in Nakdong River watershed and to supply stable water for middle area of Gyeongbuk Province. Therefore, fish investigation in up and downstream of the dam was conducted from 2004 to 2008 in order to determine any negative effect on fish community due to dam construction and to use as fundamental data for conserving species diversity and maintaining stream health. According to data analysis on water quality, temperature, dissolved oxygen, pH, suspended solids, and total E-coli had seasonal variation, but they did not significantly differ in sites. However, biological and chemical oxygen demand, chlorophyll-a, nitrogen, and phosphorus representing organic matter and nutrient concentration were higher in upper site and decreased to lower site so that they differed by site. Concentration of arsenic among the heavy metals was less than 0.05 mg $L^{-1}$, which is regulated for protection of human health in water quality standard, except for 0.092 mg $L^{-1}$ in June 2005. During the study period, the total number of fish caught from the 6 sites was 10,263 representing 7 families 19 species. Among them, dominant and subdominant species were Korean chub (Zacco koreanus, 62.5%) and Chinese minnow (Rhynchocypris oxycephalus, 10.6%) which inhabit mostly in mid and upper streams, Korea. Among the 19 species, Korean endemic species were 9 species (47.4%) including Korean slender gudgeon (Squalidus gracilis majimae), Korean dark sleeper (Odontobutis platycephala), and Korean shiner (Coreoleuciscus splendidus). There was several individuals of the $1^{st}$-class endangered species, Naktong nose loach (Koreocobitis nahtongensis), caught in 2005${\sim}$2007, and no introduced species of fish was found in entire sampling period. According to result of community analysis, dominance index decreased toward lower site, but diversity and richness indices increased toward lower site. The equation of length-weight relationship on the dominant species was TW=0.000003$(TL)^{3.2603}$. The parameter b in the equation was greater than 3.0 indicating good nutritional condition in the populations. Compared to populations of Korean chub in other streams, the population in Hwabuk Dam watershed had higher mean of condition factor by size indicating better growth rate. With fish fauna and multi-metric health assessment model in each sampling attempt, index of biotic integrity (IBI) was evaluated and it resulted mostly in good (26${\sim}$35) and excellent (36${\sim}$40) condition in all sites, and the mean of IBI was the highest in site 5. The results indicate that it is very important to study not only environmental impact assessment with fish composition but also stream health assessment in order to conserve healthy aquatic ecosystem.