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Profiling Total Viable Bacteria in a Hemodialysis Water Treatment System

  • Chen, Lihua;Zhu, Xuan;Zhang, Menglu;Wang, Yuxin;Lv, Tianyu;Zhang, Shenghua;Yu, Xin
    • Journal of Microbiology and Biotechnology
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    • v.27 no.5
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    • pp.995-1004
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    • 2017
  • Culture-dependent methods, such as heterotrophic plate counting (HPC), are usually applied to evaluate the bacteriological quality of hemodialysis water. However, these methods cannot detect the uncultured or viable but non-culturable (VBNC) bacteria, both of which may be quantitatively predominant throughout the hemodialysis water treatment system. Therefore, propidium monoazide (PMA)-qPCR associated with HPC was used together to profile the distribution of the total viable bacteria in such a system. Moreover, high-throughput sequencing of 16S rRNA gene amplicons was utilized to analyze the microbial community structure and diversity. The HPC results indicated that the total bacterial counts conformed to the standards, yet the bacteria amounts were abruptly enhanced after carbon filter treatment. Nevertheless, the bacterial counts detected by PMA-qPCR, with the highest levels of $2.14{\times}10^7copies/100ml$ in softener water, were much higher than the corresponding HPC results, which demonstrated the occurrence of numerous uncultured or VBNC bacteria among the entire system before reverse osmosis (RO). In addition, the microbial community structure was very different and the diversity was enhanced after the carbon filter. Although the diversity was minimized after RO treatment, pathogens such as Escherichia could still be detected in the RO effluent. In general, both the amounts of bacteria and the complexity of microbial community in the hemodialysis water treatment system revealed by molecular approaches were much higher than by traditional method. These results suggested the higher health risk potential for hemodialysis patients from the up-to-standard water. The treatment process could also be optimized, based on the results of this study.

Implementation of OpenGL SC Emulation Library over OpenGL (OpenGL 상에서 OpenGL SC 에뮬레이션 라이브러리 구현)

  • Baek, Nak-Hoon
    • Journal of Korea Multimedia Society
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    • v.14 no.3
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    • pp.440-448
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    • 2011
  • The needs for the OpenGL-family of the rendering library standards are highly increasing, especially for the graphical human-machine Interface on the various systems including smart phones and personal information devices. Additionally, in the case of safety-critical market for avionics, military, medical and automotive applications, OpenGL SC, the safety critical profile of the OpenGL library plays the major role for the graphical interfaces. In this paper, we represent our OpenGL SC emulation library on the OpenGL 1.x rendering pipeline which is widely available on the existing embedded systems, to provide the features of OpenGL SC standard cost-effectively. Our method can provide the OpenGL SC features at the low development cost on the embedded systems, and its implementation is also one of the fundamental elements for the emulation of embedded systems in the PC environment. Our final result now works on both of Linux-based and VxWORKS systems, showing correct execution results at the reasonable speed.

A Study on the Noise Emission Characteristics of Turbo Axial Flow Fan by Experimental Method (터보형송풍기의 소음 방사특성에 관한 실험적 연구)

  • 김동규;백종진
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2003.10a
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    • pp.271-277
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    • 2003
  • Recently as the environmental noise getting influential social problem, it is the fact that the demand on noise reduction increases with the advance of the standard of living. Therefore increasing the interest on the noise in common, it is eagerly demanded that the endeavour for reducing the noise of the rotating machinery, especially the machinery related a flowing including the household electric products, which is pointed out the primary noise source in environment. As proceeding study for fan noise, theory of fan noise property is arranged and this control method is shown. Blade passage noise of total noise spectrum. Thus in the aspect of noise reduction, noise source and identification of noise radiation characteristics of axial flow fan are demanded in detail. The sound source is analyzed by using sound pressure and sound intensity. In that time, synchronization of axial flow fan using optical sensor is executed, and to identify the location of exact noise source in the fan profile determination of recording time is proposed. In the rotating of tan, it is explained that the location of noise source exists in and by the directivity, the noise radiation pattern of axial flow fan is determined and the flow of sound is visualized in the figure of contour mapping.

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Thrust and Mixtrue Control of Liquid Propellant Rocket Engine using Q-ILC (Q-ILC를 이용한 액체추진제로켓엔진의 추력 및 혼합비 제어)

  • Jung, Young-Suk;Lim, Seok-Hee;Cho, Kie-Joo;Oh, Seung-Hyub
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2006.11a
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    • pp.139-145
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    • 2006
  • LRE(Liquid propellant Rocket Engine) is one of the important parts to control the trajectory and dynamics of rocket. The purpose of control of LRE is to control the thrust according to requiredthrust profile and control the mixture ratio of propellants fed into gas generator and combustor for constant mixture ratio. It is not easy to control thrust and mixture ratio of propellants since there are co-interferences among the components of LRE. In this study, the dynamic model of LRE was constructed and the dynamic characteristics were analyzed with control system as PID control and PID+Q-ILC(Iterative Learning Control with Quadratic Criterion) control. From the analysis, it could be observed that PID+Q-ILC control logic is more useful than standard PID control system for control of LRE.

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Self-differentiation of University Students and their Responses to Kinetic House-Tree-Person Drawings (대학생의 자아분화와 동적 집-나무-사람 그림 반응특성 연구)

  • 정윤정;최외선
    • Journal of Families and Better Life
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    • v.22 no.4
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    • pp.43-61
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    • 2004
  • The purpose of this study was to verify the usefulness of kinetic House- Tree- Person drawing as a diagnostic measure for the degree of self-differentiation, which is an essential part of college students' development. Participants for the study were four hundred thirty five(272 male and 163 female) university freshmen enrolled in 4-year colleges located in Pusan. The Participants completed a scale of self-differentiation and a Kinetic House- Tree-Person drawings test. The evaluation system for kinetic House-Tree-Person drawings was established based on the indexes of Buck(1948) and Bums(1972) and used exiting literatures as reference. The data were analyzed using means, standard deviations, t-test, one-way ANOVA, and Scheffe's test. The results obtained from the study are as follows: First, the mean level of college students' self-differentiation was 2.81, which is about average. Some significant gender differences were found in the areas of self-intergration, family projection, and emotional separation. Male students scored higher on self-intergration, whereas female students score higher on family projection and emotional separation. Second. self-differentiation was higher when the student drew a house with smoke coming out of the chimney, a single-story house with flat roof, or with detailed description of curtains, roof and roof tiles. Third, self-differentiation seemed to be higher when branches and fruits were included, when there was no expression of roots, when large crowns and branch openings were presented, and when no slant lines or base lines appeared. Forth, self-differentiation showed ㅁ higher level when the portrait shows eyes, mouth and neck without omission, when it included the whole body instead of face only, and when there was no person with just a profile, a back, or with a stiff posture, and when there was no weak and thin lines. Individuality also marked higher if a person was in motion and than one person was added. Finally, self-differentiation showed significant difference according to the overall harmony of the drawing, ordering of contents, hand pressure, the kind and shape of tree, and presence of other persons. The more harmonious the picture was, and the more family members are added, the higher the level of self-differentiation was.

Determination of Ovalbumin in Processed Foods by Immunological Methods

  • Seo, Ji-Hyun;Lee, Ju-Woon;Kang, Sin-Bok;Lee, Ha-Baik;Yook, Hong-Sun;Kim, Mee-Ree;Kim, Kee-Hyuk;Byun, Myung-Woo
    • Preventive Nutrition and Food Science
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    • v.7 no.4
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    • pp.373-377
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    • 2002
  • Allergens in processed foods may place persons with food allergies at significant risk when the labels do not Provide sufficient warnings or identification of high-risk ingredients. Because egg proteins are common food allergens, this study was carried out to identify hen's egg albumin (ovalbumin, OVA) in five commercially processed foods containing egg (custayd, cookie and pasta), and chicken meat (sausage and meatball) by immunological methods using commercially produced murine monoclonal immunoglobulin G (M-IgG), immunoblotting and enzyme linked immunosorbent assay (ELISA). Sample buffer with chelating and reducing agents was prepared and used for the preparation of the protein fractions from the foods. Most bands in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) profile (5~15% gradient gel) presented at 75 kDa below. OVA (43 kDa) in the sample lanes could not be visually observed on the gel. However, OVA in solutions prepared from custard and cookie could be detected by M-IgG, but were not detected in sausage and pasta. OVA in all samples could be quantitatively determined by the equation obtained from the standard curve by ELISA. Cookie and custard containing egg white and egg, respectively, contained very high concentrations of OVA. OVA in the other products were present in relatively low concentrations, but sufficiently high to pose possible risk of allergy, ELISA is a very sensitive and precise method for the identification and quantification of allergens in food products including allergy-inducible materials.

Comparison of Performance between Regular Drilled Shaft and Isolation Tube Drilled Shafts (일반 현장 타설 말뚝과 분리형 현장 타설 말뚝의 거동 비교)

  • Kim, Myung-Hak;O'Neill, Michael W.
    • Journal of the Korean Geotechnical Society
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    • v.16 no.1
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    • pp.211-220
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    • 2000
  • An experimental study that included detailed observation of four 305-mm-diameter drilled shafts, one reference shaft of standard design and three test shafts with isolation tubes to mitigate skin friction in the vadose zone of a clay soil profile, is described. The shafts were loaded only by naturally expanding and contraction soil over a period of 17 months. The soil at the test site was instrumented to track suction and elevation changes. Maximum ground surface movements exceeding 40 mm were observed. Heave movements of less than 1.5 mm were observed in the test shafts with isolation tubes, while movements of 5 mm were observed in the reference shaft. Unit side shear loads in the shafts protected by the isolation tubes were minimal compared to those measured in the reference shaft. This indicates that the isolation tubes were very effective.

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The Visual Performance Evaluation of the Work planes with the Automated blind Control in Small Office Spaces

  • Park, Doo-Yong;Yoon, Kap-Chun;Kim, Kang-Soo
    • KIEAE Journal
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    • v.14 no.1
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    • pp.15-22
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    • 2014
  • Among the various building envelope elements, the glass area takes up the largest portion in the office building design. However, a large area of glass can cause problems such as excessive solar radiation, thermal comfort, and glare. Thus it is important to install the glass area to an appropriate level, and control solar radiation and inflow of daylight with blind devices. This study aims to improve the visual performance of the work plane through the automatic control of the venetian blinds. A total of eight kinds of control strategies were chosen; Case 1 does not control the blinds, Case 2 with the blind slats fixed at the angle of 0 degree, Case 3 to 6 using the existing blind control programs, and Case 7 and 8 with improved blind control. Case 3 with 90 degrees had the best energy performance, but the average indoor illuminance was 113lux, which is below the standards. Cases 4 and 5 showed higher levels of interior daylight illuminance with the average of 281lux and 403lux respectively. However, the fixed angles may have difficulties controlling excessive direct sunlight coming into the room and may cause glare. Cases 6 and 7 used sun tracking angle control and cut-off angle control, and the average interior illuminance was measured 250lux and 385lux respectively. Case 8 used the cut-off angle control in an hourly manner, satisfying the standard illuminance of 400lux with an average interior illuminance of 561lux. It was evaluated to be the best method to control direct solar radiation and to guarantee proper level of interior illumination.

The design and fabricationt for ion fraction measurement of plasma generator (플라즈마발생기의 이온분율 측정 장치 설계 및 제작)

  • Lee, Chan-Young
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.11a
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    • pp.368-368
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    • 2008
  • Ion implantation has been widely developed during the past decades to become a standard industrial tool. To comply with the growing needs in ion implantation, innovative technology for the control of ion beam parameters is required. Beam current, beam profile, ion fractions are of great interest when uniformity of the implant is an issue. Especially, it is important to measure the spatial distribution of beam power and also the energy distribution of accelerated ions. This energy distribution is influenced by the proportion of mass for ion in the plasma generator(ion source) and by charge exchange and dissociation within the accelerator structure and also by possible collective effects in the neutralizer which may affect the energy and divergence of ions. Hydrogen atom has been the object of a good study to investigate the energy distribution. Hydrogen ion sources typically produce multi-momentum beams consisting of atomic ion ($H^+$) and molecular ion ($H_2^+$ and $H_3^+$). In the beam injector, the molecular ions pass through a charge-exchanges gas cell and break up into atomic with one-half (from $H_2^+$) or one-third (from $H_3^+$) according to their accelerated energy. Burrell et al. have observed the Doppler shifted lines from incident $H^+$, $H_2^+$, and $H_3^+$ using a Doppler shift spectroscopy. Several authors have measured the proportion of mass for hydrogen ion and deuterium using an ion source equipped with a magnetic dipole filter. We developed an ion implanter with 50-KeV and 20-mA ion source and 100-keV accelerator tube, aiming at commercial uses. In order to measure the proportion of mass for ions, we designed a filter system which can be used to measure the ion fraction in any type of ion source. The hydrogen and helium ion species compositions are used a filter system with the two magnets configurations.

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The effect of glossopharyngeal nerve transection on the taste buds of the rat vallate papilla (설인신경 절단이 성곽유두 미뢰에 미치는 영향)

  • Baik, Byeong-Ju;Kim, Jae-Gon;Cha, Kyung;Rho, Yong-Kwan;Park, Byung-Keon
    • Journal of the korean academy of Pediatric Dentistry
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    • v.24 no.3
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    • pp.688-703
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    • 1997
  • The effect of glossopharyngeal nerve transection on the taste buds of the rat vallate papilla was examined by using the method of DNA nick-end labeling (TUNEL) and standard electron microscopic technique at 1, 3, 5, 7, 9 days after denervation. In general, the number and size of taste buds decreased as more days passed after denervation. They started decreasing on day 3 post denervation and virtually all taste buds were disappeared on day 9 post denervation. In studies using TUNEL method, TUNEL postive cells markedly increased in their numbers one day post denervation, as compared with controls. The number of apoptotic taste bud cells per taste bud profile was averaged to be 0.64 and 0.44 for day 1 and 3 post denervation, respectively, whereas it was 0.10 in controls. In electron microscopy, apoptotic cells were identified by the presence of condensed and fragmentary nuclei in a cytoplasm, which resulted in increased density. In control rats, only few apoptotic cells were found. On days 1 and 3 post denervation, nerve fibers almost disappeared from the taste buds and some apoptotic cells were apparent. On days 7 and 9 post denervation, a few taste bud cells were still present in the epithelium of the bottom of the trench wall of the vallate papilla and most of them showed apoptotic changes. The results indicate that the death of taste bud cells in normal conditions is controlled by apoptosis and the decrease and disappearance of taste buds after denervation is also caused by apoptosis of taste bud cells.

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