• Title/Summary/Keyword: Stains

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Effect of Foilum mori on Adipocyte Differentiation

  • Lee, Geun-Won;Byun, Sang-Yo
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.8 no.5
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    • pp.299-302
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    • 2003
  • Several natural products were tested to control the differentiation of cultured human mesenchymal stem cell into adipocyte. The extent of the inhibitory effect on the conversion of adipose was measured using Oil red O staining, which stains accumulated lipid droplets in the cytoplasm of adipocyte. Of the various natural product extracts, the adipocyte differentiation was inhibited by an extract from Folium mori in the concentration range 1${\times}$10$\^$-4/∼5${\times}$l0$\^$-2/ g/mL. These results suggest that Folium mori has an inhibitory activity toward the adipose conversion, which is a major cause of obesity.

Analysis of the Survey on the Consumer's Knowledge and Laundry Habits to Microorganisms Living in Clothing (의류 중의 미생물에 대한 소비자의 지식과 세탁습관 실태조사 분석)

  • 최해운;정찬진;박명자
    • The Research Journal of the Costume Culture
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    • v.10 no.6
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    • pp.781-792
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    • 2002
  • Microorganisms living in clothing cause damage to fabric as well as unhygienic conditions with unpleasant odor fur wearers. Removal or growth of microorganisms are affected by the conditions during washing and storage. The purpose of this research was to study the consumer's knowledge and habits in laundering with respect to microorganisms in clothing. For survey method, questionnaires were administered to 580 housewives, age of 20∼60s living in Seoul. Employing 479 respondents, the data were analyzed by using descriptive statistics. The results are as follows: The level of knowledge about microorganisms of clothing was high in general, but wasn't expert level. Many people had experienced damages of textiles, clothing and unpleasant odor due to microorganisms. Fabric softeners and bleaches were rarely used for disinfection but usually used for antistatic, whitening or removal of stains. There was no relationship between laundering habits, the knowledge of microorganism, and experience of clothing damage by microorganism.

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Biological and serological characteristics of Pseudomonas aeruginosa from clinical specimens (임상검체로부터 분리한 녹농균의 생물학적 및 혈청학적 특성)

  • 임은경;김영희;김영부;오양효
    • Journal of Life Science
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    • v.10 no.2
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    • pp.157-163
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    • 2000
  • One hundred eight strains of Pseudomonas aeruginosa isolated from the patients (sputum, urine, burn skin, stool and blood) of Pusan National University hospital were tested for exonezyme production, antimicrobial susceptibility and serotyping. The results obtained were as follow: In exonezyme production test, 50 strains (46.30%) produced both protease and elastase. Thirty three strains (30.55%) did not produce any exoenzyme, 18 strains (16.67%) produced only protease and 7(6.48%) stains only produced elastase. As the result of antimicrobial susceptibility by the disc diffusion method, most strains were resistant to sulfamethoxazole (96.30%). But the resistant rate against gentamicin and ticarcillin were 47.23% and 46.30% respectively. The resistant rate to other antibiotics were less than 40%. All strains could be serologically typed. Most strains were identified as type Ⅲ: among them, 51 strains were belonged to serotype E. The correlation of serotype and exoenzyme production was not found.

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A STUDY ON THE HEMOGRAM OF PULP IN CONJUNCTION WITH PULP DISEASE (치수병변(齒髓病變)에 따르는 국소혈액상(局所血液像)에 관(關)한 연구(硏究))

  • Lee, Myung-Jong
    • Restorative Dentistry and Endodontics
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    • v.6 no.1
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    • pp.71-75
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    • 1980
  • The purpose of this study was to determine the close relation between clinical symptom and histopathologic finding in pulp. 20 samples of pulp tissue were collected from badly decayed teeth with considerable pulp vitality. Hemogram was also made with the first drop of bleedieg after total pulp extirpation (10cases) and vital pulpotomy(10cases). Histologic specimen was made routinely with extirpated pulp and stained by H&E and P.A.S. Stains. The results under microsopic examination were as follows; 1. Hemogram obtained from dental pulp with acute inflammation revealed increase of polymorphonuclear leukocyte in number, more lymphocytes and monocytes. 2. Hemogram from pulp with chronic inflammation shown the number of lymphocyte and monocyte was remarkably increased. 3. Histologi specimen obtained from teeth with severe clinical symptome showed polymorphonuclear leukocyte infiltration and the hemogram also showed polymorphonuclear leukocytes. 4. Specimen from teeth with moderate symptom showed lymphocyte infiltration and vessel dilatation underneath decayed area. Erythrocytes were massively accumulated inside of the vessel wall.

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Molecular and Epidemiological Characteristics of Infectious Bronchitis Virus Isolated in Korea (닭 전염성 기관지염 바이러스 한국분리주의 분자생물학적, 역학적 특성)

  • 송창선;이윤정
    • Korean Journal of Poultry Science
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    • v.27 no.2
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    • pp.91-98
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    • 2000
  • Phylogenetic tree constructed from the nucleotide sequences of the S1 gene showed that the 15 Korean strains of infectious bronchitis virus(IBV) examined were classified into 2 genetically distinct groups, except one respiratory strain, RB86, which was clustered with Massachusetts group. All the 5 respiratory strains belonged to Korean group I and the rest 9 nephropathogenic strains belonged to Korean group II according to the analysis, based on S1 gene sequences. Like previous classifications corresponded with the geographic origin, Korean stains were discriminated from geographically distinct reference strains of IBV. The nephropathogenic strains within Korean group IIsharing 96% homology were continuously isolated since 1990, and seemed to be genetically stable. Whereas the respiratory strains within Korean group Ⅰ sharing 88% homology were sporadically isolate since 1986m and seemed to be genetically unstable. Because we found putative accumulated point mutation as well as recombination events in Korean group Ⅰ, we discussed why genetic variations have often occurred in respiratory strains rather than nephropathognic strains.

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Leiomyoma of the Bronchus a case (기관지 평활근종: 1 수술 치험례)

  • 유영만
    • Journal of Chest Surgery
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    • v.18 no.4
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    • pp.817-821
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    • 1985
  • Benign tumor of the lung are relatively uncommon and leiomyoma among these is one of the rarest tumors. Because of salient features as well as the location of the leiomyoma, which led to the destruction of the lung and subsequent pneumonectomy, the importance of early diagnosis should be emphasized. Recently, authors experienced a leiomyoma of left main stem bronchus with complete atelectasis of the lung, resected with left thoracotomy and transverse bronchotomy in a 58 year old male. The tumor resected was composed of dense interlacing spindle cells by hematoxilin-eosin and also trichrome stains. At repeated bronchoscopic examination postoperatively, one and three months later, there was no evidence of any residual or recurrent tumors. For the universal rarity of the leiomyoma in the bronchus or lung and also there is no report in the reviews of the Journal of Korean Thoracic and Cardiovascular Surgery since volume one, 1968, authors report a case with the foreign literature reviews.

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Deformation History of Product during Forward Extrusion Process (전방압출 공정에서 제품 변형 이력)

  • 이강희;박용복
    • Transactions of Materials Processing
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    • v.10 no.1
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    • pp.75-79
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    • 2001
  • The study has been performed for the relation between die and product during forward extrusion by the experiment. Stains of the die have been given by the simple experiment using the strain gauge located at the outer surface of the die. The history of the deformation of the die and the product has been given by the experiment and Lame's formula. The inner pressure of the die causes the deformation of die that affects the accuracy of dimension as well as shape of the product. The product with accurate dimension and shape can be obtained by analysing elastic deformation of the die during the process. The deformation of the die during metal forming process has been usually predicted by the experience of industrial engineer or finite element analysis. But it is difficult to predict the dimension of the product at unloading and ejected states. In the present study, useful results for the deformation history of the die and the product were obtained through the experiment and Lame's formula in forward extrusion which can be applied to the die design for the product with accurate dimension.

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Antibiotic Resistance and Biochemical properties of Pathogenic Echerichia coli Isolated from Piglets with Diarrhea in Kyongbuk Western Area (경북서부지역 설사자돈에서 분리한 Haemolyic E. coli의 생화학적 특성 및 약제 내성)

  • 조종숙;이정아;오강희;박영구
    • Korean Journal of Veterinary Service
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    • v.16 no.2
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    • pp.150-156
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    • 1993
  • The present study was conducted to investigate the biochemical characteristics and anti-biotic resistance of pathogenic Escherichia coli (E. coli) isolated from piglets with diarrhea in Kyongbuk Western Area during the period from February to November 1992. 55 E. coli strains were isolated from 97 piglets with diarrhea and the biochemical and cultural reaction were compared with the classification criteria of Edwards and Ewing. The majority of E. coli were susceptible to amikacln, enrofloxacin and gentamicin. 51 (92.7%) out of 55 drug resistance stains carried R factor (R+) which were transferable to the recipients by conjugation.

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Ampicillin Resistance and Transferable β-Lactamase Plasmids of Gram Negative Rods Isolated from Bovine Mastitis (젖소 유방염유래(乳房炎由來) Gram 음성간균(陰性桿菌)의 Ampicillin 내성(耐性) 및 전달성(傳達性) β-Lactamase Plasmids)

  • Park, Cheong-kyu
    • Korean Journal of Veterinary Research
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    • v.25 no.1
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    • pp.61-67
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    • 1985
  • One hundred and twenty seven strains of Gram-negative rods (72 E. coli, 45 Klebsieila pneumoniae, 8 Enterobacter spp. and 2 Pseudomonas aeruginosa) isolated from bovine mastitis were examined for resistance to ampicilin, carbenicillin and cefazolin, ${\beta}$-lactamase activity and transferable ${\beta}$-lactamase plasmids. Stains resistant to ampicillin were 13.9% in E. coli, 93.3% in Klebsiella pneumoniae, 87.5% in Enterobacter. spp. and all in Pseudomonas aeruginosa, Resistance of E. coli, Klebsiella pneumoniae and Enterobacter spp. to ampicillin was due to the ${\beta}$-lactamases, but all Pseudomonas aeruginosa exhibited a high level of the non-enzymic resistance. Transferable plasmid-mediated ${\beta}$-lactamase synthesis was demonstrated in 61.9% of Klebsiella pneumoniae, 50% of E. coli and 42.9% of Enterobacter spp. The same ${\beta}$-lactamase plasmids specified different resistance levels to various ${\beta}$-lactam antibiotics in different recipients.

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A Test of Antifungal Spray Formulation Containing Nanosized Silica-Silver Particles Prepared by Using Gamma Irradiation for Practical Use to Control Indoor Fungi (감마선 조사에 의해 제조된 나노-실리카은 유무기복합 입자를 포함한 항진균성 스프레이 제제의 생활환경 저해균에 대한 실용성 검토)

  • Kim, Seong-Ho;Park, Hae-Jin;Kim, Hwa-Jung;Park, Hae-Jun
    • Journal of Radiation Industry
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    • v.2 no.3
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    • pp.149-154
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    • 2008
  • The present study described an antimicrobial spray composition comprising nanosized silica-silver particles, in which nano-silver is bound to silica molecules and a water-soluble polymer, the nanosized silica-silver particles prepared by irradiating a solution comprising a silver salt, silicate and the water-soluble polymer with radiation rays. According to a surfactant addition, the compositions were not turbid and were colorless. Also samples (cotton fabrics and wallpaper) were treated with the compositions also did not cause any stains even after drying under sunshine and at $80^{\circ}C$. Our results suggested that the spray formulation product was of practical use to control indoor fungi.