• Title/Summary/Keyword: Stains

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Taxonomic Studies of Cellulose Decomposing Fungi Imperfecti (섬유소 분해능을 가진 불완전 균류의 분류)

  • An, Won-Gun;Lee, Jae-Dong
    • The Korean Journal of Mycology
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    • v.18 no.2
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    • pp.70-76
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    • 1990
  • Twenty-one strains isolated, cellulose decomposing fungi, were identified on the basis of morphological, physiological and biochemical properties as Acremonium sp., Aspergillus sp., Chaetomium sp., Chrysonilla sp., Doratomyces sp., Fusarium sp., Gliomastix sp., Penicillium sp., Trichoderma sp., Varicosporium sp. and Verticillium sp.. The optimum tempeture for growth was in the range of $20-30^{\circ}C$. Most of the isolated stains utilized all tested carbon sources, and scarcely utilized urea as a nitrogen source. Only the strain No.2 had high activity of cellulase.

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A Study on Strain Relief in a Square Cup Drawing (정사각용기 성형에서 변형률완화에 관한 연구 1)

  • Kim, Jin-Moo;You, Ho-Young
    • Journal of the Korean Society for Precision Engineering
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    • v.16 no.2 s.95
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    • pp.31-38
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    • 1999
  • Square cups have been drawn to 20mm~60mm in depth and displacements and strains have been analysed by FEM and experiment. Displacements and strains on the corner flange of square cups have been compared with those of cylindrical cups. The results have shown that shear strains take place on corner flanges of square cups, it is necessary to adopt effective strain for comparing of stains and the effective strains on the corner flange in square cups are smaller than those in cylindrical ones.

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Manufacture of Rainbow-colored Veneer by Natural Dyeing

  • Suh, Jin Suk;Park, Ryeong Jae;Cho, Yeong Hee;Song, Eon Ja;Kim, Jong In;Park, Sang Bum
    • Journal of the Korea Furniture Society
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    • v.26 no.3
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    • pp.286-290
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    • 2015
  • The wood veneers were clearly rainbow-colored with natural dyes. As shown through Korean-style jacket with stripes of multi-colors beyond traditional obang colors (red, blue, yellow, black and white colors), eco-friendly coloring methods representing Korean colors familiar from old times could be used nobly by coloring natural wood veneer being raw material of wood products. In terms of industrialization, the study to manifest korean color, substituting chemical stains such as dye and pigment, would be necessary. In order to realize this purpose, the study about economical dyeing materials and characteristics, that is, mordant, dyeing and drying techniques showing environment-friendly coloring and high coloration level ought to be followed. In addition to this, investigating discoloration transition by fading test for interior and exterior uses would have to be carried out.

Study of Nontuberculous Mycobacteria Isolated from the Theater Environment

  • Choi, Seung-Gu;Song, Woon-Heung;Kim, Dae-Jeung;Lee, Jae-Sang
    • Korean Journal of Clinical Laboratory Science
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    • v.47 no.1
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    • pp.1-5
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    • 2015
  • The nontuberculous mycobacteria (NTM) have been found in different environmental sources. They tend to colonize different body surfaces and secretions. The purpose of this study is to evaluate the presence of NTM in the theater environment. Fifty of Theater environment sample were examined using acid-fast stain, Lowenstein-Jensen medium culture, PCR and DNA-Sequencing. 4 of 50 samples were detected as NFB in AFB stain, L-J medium culture, PCR. and then, All of 4 NTM stains identified as Mycobacterium fortitum type in DNA-sequencing result.

Four Cases of Cutaneous Tuberculosis (최근 경험한 피부결핵 4례)

  • Lee, Moo-Woong;Kwak, Tae-Hun;Choi, Jong-Soo;Kim, Ki-Hong;Kim, Mi-Jin
    • Journal of Yeungnam Medical Science
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    • v.9 no.1
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    • pp.181-188
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    • 1992
  • The incidence of the cutaneous tuberculosis has shown a steady decline over the past decades. This parallels the decreasing incidence of pulmonary tuberculosis. We experienced 5 cases of cutaneous tuberculosis from January 1990 to February 1991. We present herin 4 cases of cutaneous tuberculosis. They were 3 cases of vulgaris and 1 case of tuberculosis verrucosa cutis. Mantoux tests were done except one case and were reactive in all cases. Culture for Mycobactelium tuberculosis were done but Mycobacterium tuberculosis were not cultivated in the all cases. Histopathological findings showed tuberculoid granulomas in the dermis except one case and no acid fast bacilli were demonstrated on AFB stains.

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Bile Salt Deconjugation Activity of Lactobacillus Strains Isolated from Yogurt Products (요구르트에서 분리한 Lactobacillus들의 담즙산염 분해 능력)

  • 김근배;이재환;임광세;허철성;배형석;백영진;김현욱
    • Microbiology and Biotechnology Letters
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    • v.27 no.5
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    • pp.385-390
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    • 1999
  • To investigate bile salt hydrolase activities of the bacterial strains isolated from fermented milk products, 21 strains of Lactobacillus were tested for their abilities to produced cholic acid from taurocholic and glycocholic acids. The production of cholic acid was measured by HPLC analysis during the growth in broth media for 24hrs. All strains of Lactobacillus acidophilus and L. plantarum deconjugated both taurocholate and glycocholate, whereas none strains of L. delbrueckii subsp. bulgaricus, L. casei subsp. casei, L. casei subsp. rhamnosus, L, reuteri did. L. acidophilus stains isolated from yogurts had the higher decojugation activities on glycocholate than taurocholate, however, L. acidophilus 1009 isolated from the human intestine showed the similar deconjugation activities on both taurocholate and glycocholate.

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Isolation and Identification of High Phosphate-accumulating Bacteria (인 축정능이 우수한 세균의 분리 및 동정)

  • 신공식;고정연;최우영
    • Microbiology and Biotechnology Letters
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    • v.27 no.4
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    • pp.286-291
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    • 1999
  • By using autoradiography and phosphate medium, high phosphate accumulating bacteria were isolated from the soil of protected cultivation area and activated sludge. Selected strain, PO8, was gram-negative, rod/spherial(0.5~0.6$\times$1.0~1.2${\mu}{\textrm}{m}$ in size) and non-motile. PI4, another selected strain, was gram-negative, rod(0.4~0.5$\times$1.5~1.6${\mu}{\textrm}{m}$ in size) and motile. It also had flagella. According to their morphological, physiological and biochemical properties, the stains were identified as Acinetobacter lwoffi PO8 and Chromobacterium lividum PI4, respectively. A. lowoffi PO8 and C. lividum PI4 cultured in the P-1 medium containing 150ppm phosphate were able to uptake high phosphate up to 92% and 85%, respectively after 24 hours at 3$0^{\circ}C$ during liquid culture.

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Studies on Overglaze Stain (釉上彩色料에 關한 硏究)

  • Lee, Chong-Keun;Park, Yong-Wan
    • Journal of the Korean Chemical Society
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    • v.4 no.1
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    • pp.27-37
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    • 1957
  • High-fire stain is influenced by the conditions of glaze, firing atmosphere, etc. On the contrary, low fire stain is not influenced by those conditions but obtainable in arbitrary colors and it is indispensable for pottery decoration. It is difficult for us to study into the color in the sphere of result publication method and expression of color. Looking over the literature on this subject, I have been confronted with much difficulties, accordingly, I made a basic and systematic experiment as to six low-firing stains (blue, green, blue-green, brown black and yellow) and made it proper to be applied in these studies. On pigment, I confirmed the influence of the assistant coloring matter by mixing up the coloring matter and the assistant coloring matter in various proportions. On flux, I measured the melting point by making PbO-$SiO_2$ system and PbO-$B_2O_3-SiO_2$ system and decided the range of usage. Also I checked the coloring by making the pigment on laze with pigment and flux manufactured, and concluded the pigment and flux adaptation to it.

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Diagnosis of Tuberculosis; Serodiagnosis and Molecular Biologic Approach (결핵진단의 면역학적 및 분자생물학적 방법)

  • Shin, Wan-Shik
    • Tuberculosis and Respiratory Diseases
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    • v.39 no.1
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    • pp.1-6
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    • 1992
  • The diagnosis of tuberculosis is usually established using staining and culturing techniques. Fluorescent stains have improved the sensitivity of direct microscopy. Improved culture media coupled with radiometric means of detecting early mycobacterial growth have shortened the time needed for cultural diagnosis. Rapid immunodiagnostic techniques based on the detection of mycobacterial antigen or of antibodies to theses antigens have not, however, come into widespread clinical use. The DNA or RNA hybridization tests with labeled specific probes which have been described so far are not sensitive enough to be used for clinical speicimens without prior culturing. The advent of the polymerase chain reaction (PCR) has opened new possibilities for diagnosis of microbial infections. This technique has already been applied to a number of microorganisms. In the field of mycobacteria the PCR has been used to identify and to detect DNAs extracted from various mycobacteria. However, despite the extraordinary enthusiasm surrounding this technique and the considerable investiment, PCR has not emerged from the developmental "trenches" in the passed several years. It may be a considerable lenth of time before clinical microbiology laboratories become PCR playgrounds because many details remain to be worked out.

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Treatment of Port Wine Stain with Nodular Change (결절성 변화를 동반한 포도주색 반점의 치료)

  • Kim, Yu Jin;Lee, Jeong Min
    • Archives of Craniofacial Surgery
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    • v.9 no.1
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    • pp.27-30
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    • 2008
  • Purpose: It's difficult to treat port wine stain with nodular change in Laser therapy, and many patients with port wine stain suffer from restriction in social activity in case that port wine stain located on face. We present our experience in excision of port wine stain and skin graft in two patients who had port wine stain with nodular change on their faces. Methods: After excision of discolorized skin and nodular changed lesion of port wine stain, thick split thickness skin graft was done. Results: There was no recurred nodular hypertrophy for 3 months and 3 years of follow up periods. Conclusion: Surgical treatments of port wine stain with nodular change can lead significant improvement of lesion and social intercourse.