• Journal of Sericultural and Entomological Science
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• no.11
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• pp.63-68
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• 1970

The induction of polyhedroses in the silkworm, Bambyx mari L., was investigated treating the 5th instar larvae just after eodysis with high temperature (hot water bath at 40$^{\circ}C$ for 5 minutes or dry heat shock at 40$^{\circ}C$ for 30 minutes) and low temperature (5$^{\circ}C$ for 24 hours). The results obtained were as follows; 1. Comparing between the frequency of nuclear and cytoplasmic polyhedroses induced by cold and heat treatments (hot water bath at 40$^{\circ}C$ for 5 minutes), the induction ratio of the former is clearly less than that of the latter. But if the larvae tested with cold were left at room temperature (25$^{\circ}C$) for 30-120 minutes till the next hot water bath (40$^{\circ}C$) for 5 minutes and water bath (20$^{\circ}C$) for 5 minutes, treatments, the frequency of induced cytoplasmic polyhedrosis was more than that in the case of cold or hot water bath treatment alone. 2. The frequency of nuclear and cytoplasmic polyhedrosis induced by cold and successive heat (dry heat shock at 40$^{\circ}C$ for 30 minutes), left at room temperature (25$^{\circ}C$) ti11 the second treatment, the frequency of nuclear polyhedrosis was less than that of cytoplasmic polyhedrosis. 3. The reaction of nuclear polyhedra to stains also differs sharply from that of the cytoplasmic type. In a smear of nuclear polyhedra on a slide staining with Giemsa solution remains unstained against a stained back ground, in contrast to this, the cytoplasmic polyhedra take up stain readly.

• Journal of Environmental Health Sciences
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• v.40 no.6
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• pp.469-476
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• 2014

Objectives: The purpose of this study was to identify the stains causing infections in dental clinics by analyzing bacterial contamination, as well as to suggest improvements for infection control in dental clinics. Methods: In this study, a questionnaire survey of 47 dental hospitals and clinics located in Gyeonggi-do and Incheon, South Korea was administered from June 2013 to September 2013 and used to investigate the practice rates of infection control by dental hygienists and to analyze the bacterial contamination levels in dental offices. Results: In the studied institutions, the bacterial contamination levels of water lines were $20.9{\times}10^3$ colony forming units (CFU)/mL for three-way syringes, $12.7{\times}10^3CFU/mL$ for high-speed handpieces and $9.8{\times}10^3CFU/mL$ for gargling water. The bacterial contamination levels of surfaces were $44.9{\times}10^3CFU/mL$ in cuspidors, higher than in unit chairs ($2.9{\times}10^3CFU/mL$) and light handles ($6.7{\times}10^3CFU/mL$). The mean bacterial cell count of water lines and surfaces was relatively high in all establishments founded 11 years ago or more, and the mean bacterial cell count of waterline handpieces was $6.27{\times}10^3CFU/mL$ in establishments founded between one and five years ago, $11.16{\times}10^3CFU/mL$ six to ten years ago and $20.04{\times}10^3CFU/mL$ 11 years ago or more, which suggests that earlier foundation is associated with higher bacterial contamination levels with a statistical difference (p<0.01). Similarly, the mean bacterial cell count of cuspidors using water from water lines was also $70.16{\times}10^3CFU/mL$ in at least 11-year-old establishments, statistically significantly higher among in one- to five-year-old ($4.61{\times}10^3CFU/mL$) and six- to ten-year-old clinics ($47.89{\times}10^3CFU/mL$) (p<0.05). Conclusion: This study may be utilized to improve the bacterial contamination levels in dental offices by controlling the characteristics and environmental factors of dental offices that affect the microbial contamination of waterlines and surfaces in such institutions.

• Journal of Korean Neurosurgical Society
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• v.29 no.12
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• pp.1584-1591
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• 2000

Objective : Until now, it has been little known about the biological mechanisms associated with the genesis, growth, and rupture of intracranial aneurysm. This study was performed to investigate and understand a part of these mechanisms. Materials and Methods : Immunohistochemical stains for angiogenesis growth factors(basic fibroblast growth factor (bFGF) and vascular endothelial growth factor(VEGF)) and selected vascular wall matrix proteins(alpha smooth muscle actin(${\alpha}SMA$) and collagen Type IV) were performed in fixed sections from a normal circle of Willis artery which was taken from the autopsy specimen as a control vessel and 17 aneurysmal wall specimens which was taken during surgical clipping of aneurysms. The staining intensity and distribution of immunoreactivity to angiogenesis growth factors and selected wall matrix proteins in control vessel and aneurysmal wall were examined and compared with each other. The difference of staining intensity according to the size of aneurysm was also investigated. Results : There was no immunoreactivity to bFGF and VEGF in the control vessel. bFGF immunoreactivity was exhibited in 15 of 17 aneurysm specimens around smooth muscle cells within the media of aneurysm. VEGF immunoreactivity was also exhibited in all aneurysm specimens in patches or diffusely affecting all layers of the aneurysmal wall. The degrees of intensity of bFGF and VEGF immunoexpression were proportionate roughly to the size of aneurysm. Strong immunoexpression of both factors were noticed in large aneurysm. A regularly arranged and defined band of immunoreactivity of ${\alpha}SMA$ was noticed in the media of the control vessel, whereas diffuse, faint, irregularly arranged ${\alpha}SMA$ was noticed in the aneurysmal wall. A regularly defined band of collagen Type IV immunoreactivity was also noticed in the subendothelium of the control vessel, whereas diffuse disorganized immunoreactivity of collagen Type IV was noticed in the entire wall of the aneurysm. Conclusion : These results indicate substantial evidences of abnormal expression of angiogenesis factors and changes of selected vascular wall matrix proteins in the wall of intracranial aneurysm. The unbalanced changes of angiogenesis factors and vascular wall matrix proteins in the wall of aneurysm may be one of the biological mechanisms for the growth and rupture of aneurysm.

• Journal of Nutrition and Health
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• v.12 no.1
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• pp.59-68
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• 1979

This work was carried out to detect the enterobacterial contamination in summer kimchies and their antagonis tic activity with lactic acid bacteria during kimchi fermentation. And obtained results were as in the followings. 1) Severe cases of enterobactrial contamination were found in every kimchi material and in the first step of summer kimchi fermention. 2) Some pathogenic or food poisonous strain of Salmonellaspp. was identified among the contaminated enterobacteria. 3) Pathogenic strains of Salmonella typhi, Shigella flexneri or E. coli which were added to kimchi fermention, were contineously detected for 10days at the temperature of $10^{\circ}C$. Food poisonous strain of Vibrio parahemolyticus was more resistant than the above stains in the kimchi fermentation. 4) And in summer condition(over $30^{\circ}C$) the added pathogenic strains of enterobacteria were also detected for 2 day at pH of 4.5 kimchi fermentation. 5) The antagonistic activity of enterobacteria in lactate buffer solution of pH 4.8 was more stron than that in the kimchi fermentation at $30^{\circ}C$ of summer condition. 6) According to the above results, sanitary washing processes for every kind of kimchi material (vegitables) are required to reduce the enterobacterial concentration which is usually contaminated. And some fermentation period is also required to avoid the possible kimchi food poisoning.

• The Korean Journal of Mycology
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• v.41 no.2
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• pp.67-73
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• 2013

Microbial diversity of soil samples from ancient stone-lined tombs was investigated. The tombs, discovered at Eoryung Ocheon-Ri site, Korea, were estimated to be belonged to middle class people from an ancient country, Gaya, which existed till AD 559 at the southern part of Korea. Nine fungal stains and 70 bacterial strains were isolated from the twelve soil samples, which were collected from the tomb Nos. 5 and 6. Ribosomal DNA sequence analysis discovered 5 fungal and 22 bacterial strains belonged to 10 genus groups from the tomb No. 5 while 1 fungal and 28 bacterial strains belonged to 6 genus from the tomb No. 6. The higher microbial diversity suggests that the tomb No. 5 was constructed warmer season than the tomb No. 6. Moreover, the discovery of Staphylococcus warneri, which is found as part of the skin flora on human and animals, and Bacillus aquimaris, which is a marine bacterium and can be discovered from tidal flat, from the surface of large dagger suggests that the ancient people may use meat and seafood at the burial ceremony.

• Journal of Conservation Science
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• v.25 no.2
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• pp.219-231
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• 2009

The purpose of this study is to provide basic information necessary for the cleaning of excavated costumes. For the purpose, these researchers reviewed previous records of the actual cleaning of excavated costumes and then implemented and documented the processes of cleaning the Yeosan Song's costumes excavated at Mokdal-dong, Daejeon, which could date back to the early and mid periods of Choseon Dynasty. The excavated clothes of the family provide good examples for comparing men's costume of the 15th century with men's and women's of the mid and late 16th century. The total quantity of excavated remains were 184 and textiles were cotton, silk, hemp, ramie, and union cloth. The clothing remains were processed through wet or dry cleaning in accordance with their fabric condition and the extent to which they were worn or polluted. In detail, the excavated costumes of the Yeosan Song family were cleaned in two stages. For wet cleaning, both anionic(LAS) and nonionic(Triton X-100) surfactants were respectively used as cleaning agents and for dry cleaning, a mixture of n-hexane and n-decane(the ratio of 4 to 6) and petrolic dry cleaning solvent were used. After first cleaning, some cotton, ramie and hemp which had still the stains were processed bleaching and silk which were good condition was processed dry cleaning with the organic solvent again.

• Journal of Veterinary Clinics
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• v.27 no.3
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• pp.240-245
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• 2010

Osteonecrosis of the femoral head is an idiopathic and progressive disease. It was reported that several animal models have been used for the research of osteonecrosis. However, no standardized animal model for the study of osteonecrosis has been developed to date. This study was conducted to compare the degree of osteonecrosis of three surgically induced osteonecrosis models in rats. Twenty Sprague-Dawley rats (24 weeks old, male) were divided into three experimental groups and a control group, five heads each. Three groups were surgically induced into osteonecrosis; the ligamentum teres were cut and the periosteum of the femoral neck was stripped (Group S), the steel wire was ligated to the neck of the femoral head (Group W), and the femoral neck was tied up with a wire in the same way as in the W group, and burned by attaching the electrode tip to the wire and then the wire was removed (Group B). After two weeks, rats were sacrificed and the femoral head and neck were collected. Histological findings were evaluated with H/E stains, Safranin-O and TUNEL for osteonecrotic lesions in the bones and cartilages of the femoral head. Osteonecrosis was induced successfully in all groups (Group S, W and B) in two weeks, a short period of time. Significant necrotic changes of the cartilage were detected only in Group B. In the modified cautery model in particular, the method of removing the wire after cautery was completed in the experimental model of osteonecrosis more efficiently than any other method.

• Toxicological Research
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• v.34 no.3
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• pp.221-229
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• 2018

Tenofovir nanoparticles are novel therapeutic intervention in human immunodeficiency virus (HIV) infection reaching the virus in their sanctuary sites. However, there has been no systemic toxicity testing of this formulation despite global concerns on the safety of nano drugs. Therefore, this study was designed to investigate the toxicity of Tenofovir nanoparticle (NTDF) on the liver and kidney using an animal model. Fifteen adult male Sprague-Dawley (SD) rats maintained at the animal house of the biomedical resources unit of the University of KwaZulu-Natal were weighed and divided into three groups. Control animals (A) were administered with normal saline (NS). The therapeutic doses of Tenofovir (TDF) and nanoparticles of Tenofovir (NTDF) were administered to group B and C and observed for signs of stress for four weeks after which animals were weighed and sacrificed. Liver and kidney were removed and fixed in formal saline, processed and stained using H/E, PAS and MT stains for light microscopy. Serum was obtained for renal function test (RFT) and liver function test (LFT). Cellular measurements and capturing were done using ImageJ and Leica software 2.0. Data were analysed using graph pad 6, p values < 0.05 were significant. We observed no signs of behavioural toxicity and no mortality during this study, however, in the kidneys, we reported mild morphological perturbations widening of Bowman's space, and vacuolations in glomerulus and tubules of TDF and NTDF animals. Also, there was a significant elevation of glycogen deposition in NTDF and TDF animals when compared with control. In the liver, there were mild histological changes with widening of sinusoidal spaces, vacuolations in hepatocytes and elevation of glycogen deposition in TDF and NTDF administered animals. In addition to this, there were no significant differences in stereological measurements and cell count, LFT, RFT, weight changes and organo-somatic index between treatment groups and control. In conclusion, NTDF and TDF in therapeutic doses can lead to mild hepatic and renal histological damage. Further studies are needed to understand the precise genetic mechanism.

• Journal of Life Science
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• v.19 no.6
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• pp.799-803
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• 2009

The killing of male germ cells by radiation and other toxicants has recently been attributed to apoptosis, but a critical evaluation of the presence of the different features of apoptosis in each epithelial stage has not been performed. In this study, mouse testes exposed to radiation were examined by light microscopy and terminal transferase-mediated end labeling (TUNEL) with periodic acid-Schiff (PAS) stains to determine whether the cells were apoptotic according to several criteria. Apoptosis was easily recognized by the presence of peroxidase-stained, entirely apoptotic bodies. In the TUNEL-positive cells or bodies, the stained products correlated precisely with the typical morphologic characteristics of apoptosis as seen at the light microscopic level. The changes that occurred from 0 to 24 hours after exposing the mice to 2 Gy of gamma-rays (2 Gy/min) were examined. The numbers of apoptotic cells reached a peak at 12 hours after irradiation and then declined. The mice that received 0-8 Gy of gamma-rays were examined 8 hours after irradiation. Dose-response relationships were generated for each stage of the epithelial cycle by counting TUNEL-positive cells. The dose-response curves were linear- quadratic [y=(-0.014${\pm}$0.009)$D^{2}$+(0.31${\pm}$0.697)D+0.3575. Where y=the number of apoptotic cells per seminiferous tubule, and D=the irradiation dose in Gy, $r^{2}$=0.9] and there was a significant relationship between the frequency of apoptotic cells and the radiation dose. Although the maximum response was produced by 8 Gy, even 0.5 Gy induced marked changes. These changes were most pronounced in B spermatogonia of stage V and the spermatocyte at the mitotic cells of stage XII.

• Applied Biological Chemistry
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• v.49 no.4
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• pp.276-280
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• 2006

Traditionally fermented Cheonggukjangs were collected from Gyeonggi and Gangwon provinces and 22 strains were isolated and identified by using 165 rDNA sequences. Most of the identified strains were Bacillus subtilis and B. licheniformis, B. subtilis and B. licheniformis are dominant in the Gyeonggi area and B. licheniformis in the Gangwon area. In the growth pattern of the isolated strains, the duration of lag phase was generally 5 to 7 hours and stationary phase was reached after 23 to 40 hours of incubation. Total cell populations at the stationary phase were between $1{\times}10^6\;CFU/ml$ and $5{\times}10^7\;CFU/ml$. The fermenting ability of carbohydrates of isolates showed some differences among the regions. The isolated strains from Yong-In, Gyeonggi showed higher fermenting abilities with D-xylose, xylitol, D-tagatose and Methyl-$\alpha$-D-mannopyranoside. D-lactose, D-tagatose, D-xylose, Methyl-$\alpha$-D-mannopyranoside, amygdalin, arbutin, esculin and 2-keto-gluconate were well fermented with the An-Seong's strains; L-rhamnose, inositol, D-mannitol, D-sorbitol, celibiose and gluconate with the Kawang-Ju's stains; and D-lactose with the Odaesan's strains.