• 제목/요약/키워드: Stains

검색결과 373건 처리시간 0.028초

신고' 배 저온 저장중 발생하는 얼룩과 원인 및 방지 (Causal Factors of Black Stain during Cold Storage of Pear(Pyrus pyrifolia cv.Niitaka) and Its Postharvest Control)

  • 홍윤표;정대성;이승구
    • 한국식품저장유통학회지
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    • 제10권4호
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    • pp.447-453
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    • 2003
  • 얼룩과는 배 재배중에 사용하는 봉지 종류에 따라 수확시발생률이 다른데 흡습성과 투기성이 불량한 이중 봉지인 신문 봉지를 사용한 경우 수확시 발생률이 75%로 가장 높았으며 저온 저장중에는 습도가 높을수록 많이 발생하여 저장 180일까지 폴리에틸렌 필름 밀봉구에서 54~100% 까지 발생하였다 또한 수확 시기가 늦어질수록 얼룩과 발생이 많아져 관행 수확기보다 일주일 빠른 9월 하순에 수확한 배보다 10월 초순이나 10월 중순에 수확한 배는 저장중에 각각 1.5, 2.4배나 높은 발생률을 보였고 저장중 폴리에틸렌 필름두께에 따라서는 얼룩과 발생률이 차이가 없었다. 얼룩과의 발현 원인이 미생물로 추정되어 과피에서 분리균을 채취, 배양하여 형태적으로 관찰한 결과, 원인균은 Gloeodes pomigena와 유사한 균으로 추정되었다. 저온 저장고 내에서 0.1~0.5ppm의 오존 가스를 처리하면 저장 180일까지 얼룩과가 전혀 발생하지 않았으며 얼룩과 발생 이후에 오존 가스를 처리해도 얼룩과의 진전을 막을 수 있었다.

구강 내에 발생한 원발성 점막관련 림프양 림프종의 치험례 (PRIMARY EXTRANODAL MARGINAL ZONE B-CELL LYMPHOMA OF MUCOSA-ASSOCIATED LYMPHOID TISSUE IN THE ORAL CAVITY : A CASE REPORT)

  • 손장호;박수원;최병환;조영철;성일용;변기정
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제31권1호
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    • pp.77-80
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    • 2009
  • Mucosa-associated lymphoid tissue(MALT) lymphoma is thought to originate from marginal zone B-cells. In the WHO classification, Extranodal marginal zone lymphoma of MALT is classified B-cell Non-Hodgkin lymphoma. Common sites of MALT lymphoma include stomach, lung and the ocular-adnexa. Although less common in other sites, it is the most common low-grade lymphoma of the breast, thyroid, bowel skin and soft tissue. No strong age or gender predominance exists in MALT lymphoma. Dissemination to other sites can occur. In the oral cavity, MALT lymphoma is rare. Herein, we present a case of intra-oral MALT lymphoma. 66 year-old woman without any background of immunodeficiency or autoimmune disease admitted department of oral & maxillofacial surgery in Ulsan university hospital for evaluation of long-standing mild upper lip swelling. The lesion was completely resected and biopsied. Histological and immunohistochemical stains(CD3, CD5, CD20, CD21, CK) findings were used to confirm the lesion. Bone marrow biopsy was done and no bone marrow involvement was found. She did not receive chemotherapy and radiotherapy after surgery. No recurrence has been noted in the 22 months to date.

한국산 산민달팽이 ( Incilaria fruhstorferi ) 후촉각의 형테 및 조직화학적인 연구 (A Morphological and Histochemical Study on the Posterior Tentacle Antenna of the Korean Slug , Incilaria fruhstorferi)

  • 김영언;장남섭
    • 한국패류학회지
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    • 제12권1호
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    • pp.1-17
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    • 1996
  • Morphological and histochemical characteristics of the cells in posterior tentacle antenna of Korean slug, Incilaria fruhstorferi were observed with light microscope. The epithelium of the posterior tentacle antenna was composed of supporting cells, sensory neurons and type-a clear cell. The columnar supporting epithelium was widely distributed in the posterior tentacle antenna, and the upper end of the cell was covered with acidic mucopolysaccharide. Nerve endings of the sensory neuron were distributed between type-a clear cells. It was usually located in tentacular knob, and the number of them gradually decrdased as close as tentacular stalk. Several cilia were observed on the nerve ending. Type-a clear cells were very brightly stained with all staining used, and the neutral mucous guanules distributed in the cytoplasm. Collar cells, type-b clear cell and various types of secrdtory cells distributed in the connective tissue. The collar cells were clustering in connective tissue, and the cytoplasm were filled with neutral mucous guanules. The cells and granules were stained with dark brown by silver nitrate stain. Type-b clear cells were irregular in shape and their cytoplasms were brightly stained wth many stains used. Ten types of secretory cells evenly distributed in the connective tissue and muscle layers of the posterior tentacle antenna. The five types of the secretory cells(A, B, E, J and L)seemed to secrete acidic mucopolysaccharide, and the other five type of the cell(C, D, F, H, and L)seemed to secrete neutral mucopolysaccharide. Muscular tissue composed of well-developed thick longitudinal muscle layers and thin circular muscle layers. Type-L secretory cells clustered only in muscular layers and they contained acidic mucopolysaccharides.

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원주(原州) 회옥굴(窟)의 자연환경(自然環境)에 관(關)한 연구(硏究) (A Study on the natural environment of Hoe-ok cave in Wonju)

  • 김병우;박종범
    • 동굴
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    • 제84호
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    • pp.10-21
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    • 2008
  • 치악산에 위치한 신림면 성남2리에 있는 회옥 동굴 내부의 자연환경에 관해 2004년 11월부터 2005년 6월까지 현장조사,연구를 통해 동굴생물의 서식환경을 위한 기초 자료를 확보하고자 본 연구를 시행 하였으며, 다음과 같은 결과를 얻었다. 동굴 내 평균기온은 겨울철은 2.5$^{\circ}C$, 봄철은 5.5$^{\circ}C$, 여름철은 6.8$^{\circ}C$이며, 평균습도는 각각 82.5%, 86.0%, 86.0% 로 조사되었다. 이러한 결과는 고수동굴에 비해 온도는 10$^{\circ}C$, 습도는 4$\sim$10%정도 낮은 결과이다. 회옥굴 내부는 일반인의 무분별한 출입으로 생활쓰레기와 불에 그슬린 동굴 벽 등 훼손이 점점 더해져 가고 있다. 앞으로 지질학적인 가치평가를 더해 문화재적가치의 기준을 마련해야할 것이다.

Gum guaiac 혼합물을 이용한 혈흔검출 예비시험의 평가와 혈흔의 유전자 분석에 미치는 효과 (Preliminary Test of Forensic Identification Bloodstain Using Guaiac Mixture and It's Effect on Genetic Analysis)

  • 임채원;김양호;김진각
    • 대한임상검사과학회지
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    • 제42권1호
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    • pp.22-31
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    • 2010
  • The most common sources of biological trace material which are found in crime scene are the human bloodstains. Reliable identification in the forensic casework is important as it provides crucial insights into crime scene reconstruction and can thus contribute towards solving crimes. Blood-stains are routinely tested in forensic practise using various methods including the leucomalachite green (LMG) test, Kastle-Meyer phenolphthalein test, tetramethylbenzidine test, orthotolidine test, or the luminol chemoluminescence test with the latter cleaning attempts. All these presumptive thus indicative but not identifying tests take advantage of the peroxidase-like activity of the heme unit of the hemoglobin molecule in human blood. Therefore, false-positive results can be caused by the presence of strong oxidants, such as chlorine-containing detergents or by true peroxidases (e.g., from plants). In this study, composition for Gum guaiac was evaluated for the forensic identification of bloodstain and compared with the LMG. The sensitivity and specificity of the composition for Gum guaiac were examined more stable in bloodstain. The positive of Composition for Gum guaiac shown even with the 100,000-fold diluted bloodstain, which was no difference in comparison with LMG test. It was shown that composition for Gum guaiac was very stable to resist boiling for 20 minutes and the effect of bacteria did not affect the genetic analysis as well. The above result of the crime scene investigation, composition for Gum guaiac is easily expected to help identifying bloodstain in the evidences.

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기상 톨루엔의 생물학적 여과에 관한 실험적 연구 (Experimental Study on the Biofiltration of Toluene Gas)

  • 홍성도;명성운;최석호;김인호;이현재;구본탁
    • KSBB Journal
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    • 제15권5호
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    • pp.469-473
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    • 2000
  • 본 연구는 실험실 규모의 biofilter를 이용하여 공기 중에 혼합되어 있는 대표적인 휘발성 유기화합물인 toluene기체를 제거하는 실험이다. 생물여과실험은 일반적인 조건하에서 오 염된 공기를 바이오필터의 상단으로부터 하단으로 흐르게 하 여 수행하였다. 반웅기 컬럼의 내부는 peat와 calstone (부피 비 5:3) 혼합물로 충진 되었으며, 충진재는 반응기에 채우기 전에 미생물 (Pseudomonas putida type A)과 잘 혼합하였다. 접종된 미생물은 충진 고체충에 고정화되어 생물막을 형성하 게 된다. 바이오필터는 다양한 툴루엔 농도와 유속조건에서 약 90일 이상 운전 되었으며, 최대 제거량은 유입 toluene량 $30 g/m^3hr$에서 $20 g/m^3hr$임을 알 수 있었다. 오염왼 공기는 체류시간 1-2 min에서 제거율이 20-90%끼지 변화하는 것을 보여 주었다 한편 운전중에 발생하는 압력강하는 $1.062 cmH_2O/m$ 정도로서 반응기 전체를 통하여 무시할 수 있을 정도로 낮았다.

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다각근사법을 이용한 도로방향 결정 (Decision of Road Direction by Polygonal Approximation.)

  • 임영철;박종건;김의선;박진수;박창석
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1996년도 하계학술대회 논문집 B
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    • pp.1398-1400
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    • 1996
  • In this paper, a method of the decision of the road direction for ALV(Autonomous Land Vehicle) road following by region-based segmentation is presented. The decision of the road direction requires extracting road regions from images in real-time to guide the navigation of ALV on the roadway. Two thresholds to discriminate between road and non-road region in the image are easily decided, using knowledge of problem region and polygonal approximation that searches multiple peaks and valleys in histogram of a road image. The most likely road region of the binary image is selected from original image by these steps. The location of a vanishing point to indicate the direction of the road can be obtained applying it to X-Y profile of the binary road region again. It can successfully steer a ALV along a road reliably, even in the presence of fluctuation of illumination condition, bad road surface condition such as hidden boundaries, shadows, road patches, dirt and water stains, and unusual road condition. Pyramid structure also saves time in processing road images and a real-time image processing for achieving navigation of ALV is implemented. The efficacy of this approach is demonstrated using several real-world road images.

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21세기 패션에 수용된 추상표현주의 기법에 관한 연구 (A Study of Abstract Expressionist Techniques in 21st Century Fashion)

  • 김선영
    • 한국의류학회지
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    • 제33권9호
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    • pp.1430-1440
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    • 2009
  • This study is on the abstract expressionist techniques among the art activities variously expressed in modern fashion. Its significance lies in making fashion artistic through the combination of fashion and art in contributing to the development of creative fashion culture. In terms of method, documents are used to characterize the concept of abstract expressionism, the works of representative artists, and the panting techniques in relation to modem art based on existing literature. Fashion-related anthology, domestic, and foreign fashion magazines were used to analyze the abstract expressionism applied to modern fashion since 2000. According to the findings, the abstract expressionism applied to modern fashion showed artistic expressions with the abstract beauty by chance, using only the images or techniques similar to those in abstract expressionism. Expressionist works had abstract and dynamic images, as they were given a third dimension through the process of being worn on the human body. Second, details or decorative factors were excluded to ensure the maximum space for expression, modem images were displayed using the simple forms such as silhouettes (spacious or dense) and the beauty of harmony was shown that had beauty emphasized by the expression effects of textile design, the division of space, and the composition of colors. Third, the action painting techniques in modern fashion were used for textile designs printed on the surface of clothes, and the dynamic character of the design was shown by the duplication and juxtaposition of stains created by chance. The color field abstract techniques were shown through printing, texture, and dying, in addition the intense and pure abstract images were displayed by treating clothes like large screens.

감염 근관에서 분리된 연쇄구균의 16S Ribosomal DNA 중합효소 연쇄반응과 제한효소 절단길이 다형성에 관한 연구 (POLYMERASE CHAIN REACTION AND RESTRICTION FRAGMENT LENGTH POLYMORPHISM OF 16S RIBOSOMAL DNA OF STREPTOCOCCI ISOLATED FROM INFECTED ROOT CANALS)

  • 정희일;임미경
    • Restorative Dentistry and Endodontics
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    • 제20권2호
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    • pp.577-609
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    • 1995
  • Bacteria have been regarded as one of the most important factors in pulpal and periapical diseases. Streptococci are frequently isolated facultative anaerobes in infected root canals. Recently molecular biological techniques have been rapidly progressed. This study was designed to apply the molecular biological tools to the identification and classification of streptococci in the endodontic microbiology. Streptococci isolated from infected root canals were identified with both Vitek Systems and API 20 STREP. Identification results were somewhat different in several strains of streptococci. Eighteen streptococci and enterococcal was difficult so to digest plasmid DNA using Hind III and EcoRI to differentiate strains by restriction enzyme analysis of plasmid DNA. 16S rDNA of chromosome was amplified by polymerase chain reaction(PCR) and then restricition fragment length polymorphism(RFLP) using several restriction enzymes was observed. The molecular mass of 16S rDNA of chromosomal DNA was approximately 1.4kb. There were three to five RFLP patterns using eight restriction enzymes. RFLP patterns digested with CfoI which recognizes four base sequences were identical in all stains. Hind III which recognizes six base sequences could not digest the 16S rDNA. Restriction enzymes which recognize five base sequences were suitable for RFLP pattern analysis. At least three different restriction enzymes were needed to compare each strains. 16S rDNA PCR-RFLP was simple and rapid to differentiate and classify strains and could be used in the epidemiological study of root canal infections.

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원발성 폐암에서 종양억제유전자 RB와 p53 단백질 발현양상과 예후와의 상관관계 (Expression Pattern of RB and p53 Proteins and its Correlation with Prognosis in Primary Lung Cancer)

  • 이상용;허혜경;최필조;우종수;홍숙희
    • Journal of Chest Surgery
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    • 제29권11호
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    • pp.1223-1231
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    • 1996
  • 72예의 원발성 폐암조직에서 두 종양억제유전자 RB와 p53의 단백질 발현 빈도를 면역조직화학적 방법으로 검출한 결과 RB 단백질 발현은 38예 (52.8%)에서 감소하거나 소실되었고, 변이형 p53 단백질은 35예(48.6%)에서 발현을 보였다. 이들의 발현 빈도는 폐암의 조직형에 따라 유의한 차이를 보여 RB는 선암에서 그리고 p53은 편평세포암에서 발현율이 높았다(p<0.05). 그러나 임상 병기와는 두 단백질의 발현율과는 상관이 없었다. 폐암 환자의 2년 생존율은 두 단백질 발현의 변화가 동반된 군(RB-/p53+)에서 22.4%, 두 단백질 발현의 변화가 전혀 없는 군(RB+/p53-)에서 63.1%로 두 군 사이에 통계적으로 유의한 차이를 보였다(p<0.05).본 연구에서 RB와 p53단백질의 발현의 유무가 원발성 폐암 환자의 생존율과 관련이 있음을 나타내었고, 특히 RB가 원발성 폐암의 예후에 가장 큰 영향을 미치는 인자임을 나타내었다.

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