• 한국가구학회지
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• 제25권4호
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• pp.319-324
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• 2014

Red pine is a popular species for making a cutting board in Korea, but easily sap stained. Heat treatment could improve its quality by darkening and equalizing the color of sap stained wood. The color change of sap stained red pine boards was investigated by heat treatment at $190^{\circ}C$. It was observed that the color of heat treated boards got darker and it made the color of sap stain vanished. A colorimeter was used to measure color indexes. It was revealed that the values of the lightness ($L^*$) and the yellowness ($b^*$) decreased as heat treatment repeated while the values of the redness ($a^*$) increased. The average of the color difference (${\Delta}E^*$) between the control and 1st heat treated boards was 16.1, which could be expressed as 'Extremely different' while that between the 1st and 2nd heat treated boards was 8.3, which could be expressed as 'Considerably different'. The fact that heat treatment equalized the color of boards was confirmed by a statistical analysis.

• The Korean Journal of Physiology and Pharmacology
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• 제10권4호
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• pp.193-198
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• 2006

Many gastrointestinal muscles show electrical oscillation, so-called 'slow wave', originated from interstitial cells of Cajal (ICCs). Thus, a technique to freshly isolate the cells is indispensable to explore the electrophysiological properties of the ICCs. To apply an enzyme solution on the serosal surface for cell isolation, the intestine was inverted and 0.02% trypsin solution and 0.04% collagenase solution were applied to serosal cavity. After the enzyme treatment, mucosal layer was removed and longitudinal muscle layer was gently separated from the rest of tissue. The thin layer was stretched in the recording chamber and mounted on an inverted microscope. Using ${\beta}-escine$, perforated whole cell patch clamp technique was used. Under a microscope, the tissue showed smooth muscle cells and interstitial cells around the myenteric plexus. Under voltage clamp condition, three types of membrane potential were recorded. One group of interstitial cells, which were positive to methylene blue and CD34, showed spontaneous outward current. These cells had bipolar shape and were considered as fibroblast-like cells because of their peculiar shape and arrangement. Another group, positive to c-kit and methylene blue, showed spontaneous inward current. These cells had more rounded shape and processes and were considered as ICCs. The third, positive to c-kit and had granules containing methylene blue, showed quiet membrane potentials under the voltage-clamp mode. These cells appeared to be resident macrophages. Therefore, in the freshly isolated thin tissue preparation, methylene blue could easily identify three types of cells rather than morphological properties. Using this method, we were able to study electrical properties of fibroblast and residential macrophage as well as myenteric ICCs.