• 한국미생물·생명공학회지
• /
• 제23권5호
• /
• pp.544-549
• /
• 1995

The alkaline elastase is an extracellular serine protease of the alkalophilic Bacillus strain Ya-B. To increase the gene copy number and the production level of the alkaline elastase Ya-B, we designed, on the B. subtilis chromosome, a gene amplification of the 10.6 kb repeating unit containing amyE, aleE (alkaline elastase Ya-B gene) and tmrB. The aleE was inserted between amyE and tmrB, and B. subtilis APT119 strain was transformed with this amyE-aleE-tmrB-junction region fragment. As a result, we succeeded in obtaining tunicamycin-resistant (Tm$^{r}$) transformants (Tf-1, Tf-2) in which the designed gene amplification of 10.6 kb occurred in chromosome. The transformants showed high productivity of $\alpha $-amylase and alkaline elastase Ya-B. The copy number of the repeating unit (amyE-aleE-tmrB) was estimated to be 25, but plasmid vector (pUC19) was not integrated. The amplified aleE of chromosome was more stable than that of plasmid in absence of antibiotics.

• Journal of Multimedia Information System
• /
• 제1권1호
• /
• pp.77-85
• /
• 2014

Usually, the skin pigmentation detection and diagnosis are made by clinicians. In this process it is subjective and non-quantitative. We develop an approach to detect and measure the different pigmentation lesions base on computer vision technology. In the paper we study several usually used skin-detecting color space like HSV, YCbCr and normalized RGB. We compare their performance with illumination influence for detecting the pigmentation lesions better. Base on a relatively stable color space, we propose an approach which is RGB channels vector difference characteristic for the detection. After the object region detection, we also use the difference to measure the difference between the lesion and the surrounding normal skin. From the experiment results, our approach can effectively detect the pigmentation lesion, and perform robustness with different illumination.

• Journal of Power Electronics
• /
• 제14권6호
• /
• pp.1208-1216
• /
• 2014

Mismatch between switching frequency and circuit parameters often occurs in industrial applications, which would lead to instability phenomena. The bifurcation behavior of $V^2$ controlled buck converter is investigated as the pulse width modulation period is varied. Nonlinear behavior is analyzed based on the monodromy matrix of the system. We observed that the stable period-1 orbit was first transformed to the period-2 bifurcation, which subsequently changed to chaos. The mechanism of the series of period-2 bifurcations shows that the characteristic eigenvalue of the monodromy matrix passes through the unit circle along the negative real axis. Resonant parametric perturbation technique has been applied to prevent the onset of instability. Meanwhile, the extended stability region of the converter is obtained. Simulation and experimental prototypes are built, and the corresponding results verify the theoretical analysis.

• 한국전기전자재료학회논문지
• /
• 제18권3호
• /
• pp.199-205
• /
• 2005

Polycrystalline cadmium sulfide(CdS) thin films were deposited on glass substrate by chemical bath deposition(CBD) and vacuum evaporation (VE) techniques. VE-CdS films consisted primarily of hexagonal phase, whereas CBD CdS films containing primarily the cubic form. VE-grown films were shown to have better crystallinity than CBD-grown films. The grain size of the CBD films is smaller than the ones of VE films. VE-CdS films exhibited relatively high transmittance in the above-gap region and band gap compared with CBD films. However, CdTe solar cells with these low quality CBD-CdS layers yield higher and more stable characteristics. Current-voltage-temperature measurements showed that the current transport for both cells was controlled by both tunneling and interface recombination but the cells with CBD-CdS displayed less tunneling.

• 한국고분자학회:학술대회논문집
• /
• 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
• /
• pp.241-241
• /
• 2006

Absolute configuration of carboxylic acids, including amino acids can be determined by circular dichroism (CD) exciton chirality method. This method employs cyclic oligopyrroles able to form stable complexes with carboxylic acids. Addition of carboxylic acids to the oligopyrroles induce CD spectrum at the macrocycle absorption region and in which the sign of the $1^{st}$ Cotton effect is determined solely by the absolute configuration of the carboxylic acid. The basicity of the pyrrole nitrogen can be controlled by macrocycle substitution thus yielding more sensitive chirality sensors.

• 대한용접접합학회:학술대회논문집
• /
• 대한용접접합학회 2002년도 Proceedings of the International Welding/Joining Conference-Korea
• /
• pp.211-216
• /
• 2002

To investigate transition region in welded overlay relating to disbonding crack, the effect of vanadium addition on disbonding of Cr-Mo steels overlay welded with austenitic stainless steel was studied. V modified Cr-Mo steels have a higher resistance to disbonding than V free Cr-Mo steel. One reason is due to the fact that fine vanadium carbide precipated in base metal traps hydrogen and thus decreases the susceptibility to the disbonding. The second is related to the higher stability of the vanadium and stable carbides formed during PWHT, in which the carbon diffusion to the interface is lower than for V free Cr-Mo steel. Decreasing the carbon content at the interface of the weld overlay shows good resistance to the disbonding. Hence, it is important to control the carbon content at the interface of the weld overlay.

• 제어로봇시스템학회:학술대회논문집
• /
• 제어로봇시스템학회 1993년도 한국자동제어학술회의논문집(국내학술편); Seoul National University, Seoul; 20-22 Oct. 1993
• /
• pp.710-717
• /
• 1993

In this paper, we focus upon the design and applications of adaptive fuzzy-neuro controllers. An intelligent control system is proposed by exploiting the merits of two paradigms, a fuzzy logic controller and a neural network, assuming that we can modify in real time the consequential parts of the rulebase with adaptive learning, and that initial fuzzy control rules are established in a temporarily stable region. We choose the structure of fuzzy hypercubes for the fuzzy controller, and utilize the Perceptron learning rule in order to update the fuzzy control rules on-line with the output error. And, the effectiveness and the robustness of this intelligent controller are shown with application of the proposed adaptive fuzzy-neuro controller to control of the cart-pole system.

• 제어로봇시스템학회:학술대회논문집
• /
• 제어로봇시스템학회 2005년도 ICCAS
• /
• pp.1871-1876
• /
• 2005

In this paper, We present a new nonlinear adaptive control law using a disturbance accommodating control (DAC) observer for a Japanese automatic landing flight experiment vehicle called ALFLEX. A future spaceplane must have ability to deal with greater fluctuations in the stability and control derivatives of flight dynamics, because its flight region is much wider than that of conventional aircraft. In our previous studies, digital adaptive flight control systems have been developed based on a linear-parameter-varying (LPV) model depending on dynamic pressure, and obtained good simulation results. However, under previous control laws, it is difficult to accommodate uncertainties represented by disturbance and nonlinearity, and to design a stable flight control system. Therefore, in this study, we attempted to design a nonlinear adaptive control law using the DAC Observer and inverse dynamic methods. A good tracking property of the obtained system was confirmed in numerical simulation.

• 한국정보디스플레이학회:학술대회논문집
• /
• 한국정보디스플레이학회 2006년도 6th International Meeting on Information Display
• /
• pp.1587-1590
• /
• 2006

Through the engineering of recombination region and energy transfer in organic light emitting device, blue and red light emitting device with good color stability has been successfully obtained. A Color control layer (CCL), which emits green light through the energy transfer from the emission layers, has been introduced into the blue and red light emitting device for RGB white OLED. The RGB white OLED showed the current efficiency of 13 cd/A and the CIE coordinates of (0.33, 0.38) at $1000\;cd/m^2$. The device exhibited very stable spectrum with respect to operating current density and the CIE coordinates varied from (0.34, 0.38) to (0.31, 0.37) for $100-22000\;cd/m^2$.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 1984년도 하계학술회의논문집
• /
• pp.226-229
• /
• 1984

A novel high intensity charging device has been developed for the control of the submicron particles, such as the diesel soot particulates, which are very hard to charge highly by any means of the conventional charging device. Having new corona electrodes of a multineedle disk with the corona field-control electrodes in the outer-cylinder electrode, extremely intense and stable coronas on there sharp points expanding both radially and axially are established in the corona charging region between the multi-corona -needles and an outer-cylinder electrode. As a result, the maximum corona field intensity and current density of the charging device of the standard one on soot load in laboratory tests have been 8.5KV/cm(E=$2V_m$/D(1nD/d)) and $6.5{\mu}A/cm^2$ which enhance greatly the charging of soot particles about several 100 times higher than those obtained in conventional cylinder precipitators.