• Title/Summary/Keyword: Stabilizing effect

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Stability Enhancement of hGM-CSF in Transgenic Nicotiana tabacum Suspension Cell Cultures

  • Lee, Sang-Yoon;Cho, Jong-Moon;Kim, Dong-Il
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.8 no.3
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    • pp.187-191
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    • 2003
  • Proteolytic enzymes existing in plant cell cultured media are the major reason for the loss of secreted human granulocyte-macrophage colony-stimulating factor (hGM-CSF). The addition of pepstatin, aprotinin and PMSF relatively decreased the proteolytic degradation of hGM-CSF in a conditioned medium, but sufficient prevention against the proteolytic activity could not be obtained with chemical protease inhibitors. Gelatin, as a competitive substrate for protease, showed a stabilizing effect in a conditioned medium. Compared to the initial hGM-CSF concentration in a conditioned medium. with 10 g/L of gelatin, 68% of the hGM-CSF remained after 5 days. In a cell culture experiment, 5 g/L of gelatin significantly stimulated the hGM-CSF production and accumulation in culture media, with no growth inhibition. compared to the controls (4.72 $\mu\textrm{g}$/L), the extracellular hGM-CSF level could be increased to 39.78 $\mu\textrm{g}$/L with the addition of 5 g/L of gelatin.

Study on Prediction and Control of Wind-Induced Heel Motion of Cruise Ship (바람 하중에 의한 크루즈선의 횡경사 예측 및 제어에 관한 연구)

  • Kim, Jae-Han;Kim, Yonghwan;Kim, Yong-Soo
    • Journal of the Society of Naval Architects of Korea
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    • v.50 no.4
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    • pp.206-216
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    • 2013
  • The present study considers the prediction of wind-induced heel of cruise ship and its stabilization. Wind load in ocean exerts on the surface of superstructure of cruise ship, which causes the heel moment on the ship. The calculation of wind load starts from choosing wind speed profile, so that the logarithmic wind profile model is applied in this study. Heel moment by wind load is calculated by adopting approximate formulation and applied to the ship motion analysis in time domain. Motion stabilizers, such as stabilizing fin and U-tube tank, are considered to reduce the heel effect as well as excessive roll motion. From this study, it is expected that the present method can be applied to the prediction and stabilization of the heel motion of cruise ships.

Performance evaluation of differently structured RCE-DR GdBCO coated conductor tapes under uniaxial tension at 77 K

  • Diaz, Mark Angelo E.;Shin, Hyung-Seop;Jung, Ho-Sang;Lee, Jaehun
    • Progress in Superconductivity and Cryogenics
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    • v.24 no.1
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    • pp.13-17
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    • 2022
  • The mechanical properties of REBCO coated conductor (CC) tapes under uniaxial tension are mainly determined by the thick layer Components like the substrate and the stabilizer. Depending on the applications of the CC tapes, it is also needed to externally reinforce thin metallic foils to one side or both sides of the CC tapes. This study investigated the effect of additional stabilizer layers or lamination on the electrical resistivity and electromechanical properties in RCE-DR processed GdBCO CC tapes with different structures. The strain/stress tolerance of Ic in differently processed 12 mm-wide REBCO CC tapes under uniaxial tension at 77 K and self-field could be determined by the loading-unloading scheme. As a result, Sn-Cu stabilized CC tape showed a significant decrease in mechanical properties due to its soft but thick stabilizing layer. However, similar electromechanical properties have been observed on both Sn-Cu and Sn-stabilized CC tapes, the Ic degradation behavior was independent of whether the CC tape has an external reinforcement or different stabilizing layers.

A study of feedback resister series effects for stabilizing the gain of the photomultiplier with counting rate increase (계수율 증가에 따른 광증배관의 이득안정을 위한 궤환저항 효과에 관한 연구)

  • 천희영;신현국
    • 전기의세계
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    • v.29 no.7
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    • pp.455-459
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    • 1980
  • The gain change of the photomultiplier which occurs as the count rate increased was examined. and simple feedback resistor series was introduced between 7th and 9th dynode for compensating the gain change. The effect of the feedback resistor series was considerably good to reduce the gain change. With the feedback resistor, the gain change was only 1.9%, when count rate was increased from 514 CPS (counts per second) to 1250 CPS. Without the feedback resister, the gain change was 6.9%, when the count rate was increased from 530 CPS to 1250 CPS.

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L-Ascorbic Acid-2-Phosphate Mg염의 합성 및 응용

  • 양창모
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.13 no.1
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    • pp.29-35
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    • 1987
  • Purely synthesized L-ascorbic acid 2 phosphate Mg salt (1 AsA PMg) improved the weak point of ascorbic acid which is easily decomposed in water solution. This compound is hydrolyzed with phosphatase of skin to corresponding ascorbic acid giving Vitamine C activities. The buffer solution of potassium acetate 0.5% and citric acid 0.005% and the sodium sulfite respectively showed good stabilizing effect of the AsA PMg solution. Compared to the other ascorbic acid derivatives the good solubility of AsA PMg gives broad application to cosmetic field.

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A PMO Approach to Orbital Interactions Through Space and Through Bonds

  • Lee, Ik-Choon
    • Bulletin of the Korean Chemical Society
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    • v.3 no.4
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    • pp.140-144
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    • 1982
  • Through-space and through-bonds interactions between two nonbonding orbitals are shown to be first-order and second-order perturbations of nonbonding orbitals by the framework sigma orbitals. Through-space interactions for diamines and triplet diradicals are always destabilizing, but through-bonds interactions are stabilizing owing to their coupling with through-space effect. Anomalies in level ordering and magnitudes of energy splitting can be rationalized with the present approach. Orbtial interactions predicted with the PMO method were found to be consistent with those obtained by STO-3G calculations and photoelectron spectroscopy.

Image Stabilization Algorithm for Close Watching UAV(Unmanned Aerial Vehicle) Aystem (근접감시용 무인항공기 시스템을 위한 영상 안정화 알고리즘)

  • Lee, Hong-Suk;Lee, Tae-Yeoung;Kim, Byoung-Soo;Ko, Yun-Ho
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.47 no.6
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    • pp.10-18
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    • 2010
  • This paper proposes an image stabilization algorithm for close watching UAV(Unmanned Aerial Vehicle) using motion separation and stabilization mode. The motion of UAV is composed of its actual navigating motion and unwanted vibrating motion so that image sequences obtained from UAV are shaken randomly. In order to stabilize these images we separate the vibrating motion component from UAV motion and remove the effect caused by it from image sequences. In the proposed algorithm the motion and global intensity change of two consecutive images are modeled with 6 motion parameters and 2 intensity change parameters respectively. These modeled parameters are estimated by non-linear least square method based on Gauss-Newton algorithm. The vibrating motion component is separated from the estimated motion using IIR filtering and the geometric deformation caused by it is removed from image sequences. In order to apply the proposed method to real aerial image sequences with many abrupt changes of camera view, we proposed a stabilizing method using two different modes named as stabilizing and non-stabilizing mode. Experimental results show that the accuracy of motion estimation is 99% and the efficiency of removing the vibrating motion component is 90%. We apply the proposed method to real aerial image sequences and verified its stabilizing performance.

MO Studies of Configuration and Conformation (XV). Through-Space and Through-Bond Interactions In Ethylene Diamine (배치와 형태에 관한 분자궤도론적 연구 (제15보). 에틸렌 디아민의 Through-Space 및 Through-Bond 상호작용)

  • Ikchoon Lee;Chang Kook Sohn;Chang Hyun Song
    • Journal of the Korean Chemical Society
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    • v.26 no.2
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    • pp.65-72
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    • 1982
  • STO-3G level computations were performed on n-propylamine, n-propylamine radical and cis-and trans-ethylene diamines in order to investigate structural contributions of (n${\pi}$/m) and (n-${\sigma}^*$) structures to the energy variations accompanying the conformational changes. It was found that (5${\pi}$/5) and (4${\pi}$/4) structures had attractive and repulsive nonbonded interactions, respectively, which were approximately additive. anti(n-${\sigma}^*$) structures had more stabilzing hyperconjugative interactions than syn(n-${\sigma}^*$) structures, but due to the large internuclear repulsion the net effect was destabilizing inthe former in contrast with the net stabilizing contribution in the latter. Moreover it was found that the stabilizing ${\pi}$-nonbond structure, (5${\pi}$/5) was always cooperatively reinforced by the more stabilizing anti(n-${\sigma}^*$) interaction, whereas the destabilizing (4${\pi}$/4) structure was accompanied by the less stabilizing syn(n-${\sigma}^*$) interaction. This type of cooperativity was found general through-bond interaction of the terminal lone pair lobes split the energy levels into two, $n_+ = \frac{1}{\sqrt{2}}(n_1 + n_2)$ and $n_- = \frac{1}{\sqrt{2}}(n_1 - n_2)$, the latter being the lower level, which can be shown using simple overlap patterns of the two lobes with a common vicinal ${\sigma}^*$ orbital.

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Stability of Inclined Premixed Planar Flames (기울어진 예혼합 평면화염의 안정성)

  • Lee, Dae-Keun;Kim, Moon-Uhn;Shin, Hyun-Dong
    • 한국연소학회:학술대회논문집
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    • 2004.11a
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    • pp.97-106
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    • 2004
  • Stability of laminar premixed planar flames inclined in gravitational field which generate vorticity is asymptotically examined. The flame structure is resolved by a large activation energy asymptotics and a long wave approximation. The coupling between hydrodynamics and diffusion processes is included and near-unity Lewis number is assumed. The results show that as the flame is more inclined from the horizontal plane it becomes more unstable due to not only the decrease of stabilizing effect of gravity but also the increase of destabilizing effect of rotational flow. The obtained dispersion relation involves the Prandtl number and shows the destabilizing effect of viscosity. The analysis predicts that the phase velocity of unstable flame wave depends on not only the flame angle but also the Lewis number. For relatively short wave disturbances, still much larger than flame thickness, the most unstable wavelength is nearly independent on the flame angle and the flame can be stabilized by gravity and diffusion mechanism.

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Effect of Activation Energy and Crystallization Kinetics of Polyethylenes on the Stability of Film Casting Processes

  • Lee, Joo-Sung;Cho, Joon-Hee
    • Korea-Australia Rheology Journal
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    • v.21 no.2
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    • pp.135-141
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    • 2009
  • Effect of activation energy and crystallization kinetics of polyethylenes (PEs) on the dynamics and stability has been investigated by changing rheological properties and crystallization rate in film casting process. The effect of changes of these properties has been shown using a typical example of short-chain branching (SCB) in linear polyethylenes. SCBs in linear polymers generally lead to the increase of the flow activation energy, and to the decrease of the crystallization rate, making polymer viscosity lower in the case of equivalent molecular weight. In general, the increment of the crystallinity of polymers under partially crystallized state helps to enhance the process stability by increasing tension, and lower fluid viscoelasticity possesses the stabilizing effect for linear polymers. It has been found that the fluid viscoelasticity plays a key role in the control of process stability than crystallization kinetics which critically depends on the cooling to stabilize the film casting process of short-chain branched polymers operated under the low aspect ratio condition.