Photosensitizing activities of some photosensitizers (PS) for the artificial diminution of pesticide residues on horticultural crops were investigated. Five fungicides, iprodione, bitertanol, chlorothalonil, myclobutanil, and dichlofluanid were sprayed on apple and cucumber, followed by the application of each selected photosensitizer, and the samples were collected 0, 1, 3, 7, 15 days after the photosensitizer application and analyzed for the residual amounts. Of the 40 photosensitizers tested, six selected on the basis of the eliminating effect of pesticide residues were PS-1 (aromatic ketone), PS-2 (aromatic amine), PS-3 (quinone), PS-4 (inorganic compound), PS-5 (organic acid salt), and PS-6 (semiconductor photocatalyst). The residual amount of iprodione after 15 days of the application of PS-1 was 74% of that of the control. For bitertanol, the residual amount after 15 days of the application of PS-1 accounted for 78% of that of the control. The residual amounts of chlorothalonil after 1 day of the application of PS-1 and PS-2 accounted for 56 and 54% of those of the control, respectively. The residual amounts of iprodione on cucumber after 3 days of the application of the photosensitizers PS-1 and PS-2 were 44 and 67% of those of the untreated control, respectively. For myclobutanil, the residual amount after 15 days of the application of PS-6 accounted for 45% of that of the control. In case of dichlofluanid, the residual amount after 3 days of the application of PS-1 accounted for 44% of that of the control. Based on the results, PS-1 turned out to be the most promising photosensitizer for the accelerated photodegradation of the above fungicides on apple and cucumber.
Kim, Hea Na;Kim, Seong Beom;Choi, Eun;Woo, Min Ji;Kim, Ji Yoon;Saravanan, Manoharan;Hur, Jang Hyun
The Korean Journal of Pesticide Science
/
v.18
no.3
/
pp.123-129
/
2014
The present work was aimed to determine the pre-harvest residue limits (PHRLs) and the safety management of commonly used pesticides namely buprofezin and penthiopyrad on oriental melon (Cucumis melon var. makuwa). In this study, the buprofezin (diluted two thousand fold) and penthiopyrad (diluted four thousand fold) were sprayed single time on oriental melon in the cultivation areas Sangju (site 1) and Sungju (site 2). Oriental melon were randomly collected from the both areas at the end of 0 (2 hours after pesticides spaying), 1, 2, 3, 5, 7, 9 and 10 days. For analysis, each samples were partitioned twice (80 and 70 mL) with dichloromethane and purified by florisil SPE cartridge. Finally, the residual amounts of both pesticides in all samples were analyzed using gas chromatography/nitrogen phosphorus detector (GC/NPD). In this study, the method limit of quantification (MLOQ) for both buprofezin and penthiopyrad in oriental melon was found to be $0.01mg\;kg^{-1}$ and their recovery levels were 91.1~98.6% and 90.0~104.6%, respectively. Further, the calculated biological half-life for buprofezin and penthiopyrad in oriental melon were 3.9 and 3.5, and 3.0 and 2.7 days in site 1 and 2, respectively. The results of this study found that the PHRLs for buprofezin and penthiopyrad were 4.24 and $2.31mg\;kg^{-1}$, respectively at 10 days before harvest. Consequently, the present study suggest that the residual amounts of both pesticides will be lower than the maximum residue limits (MRLs) when oriental melon is harvested.
This study was carried out to investigate the residue patterns of two insecticides, lambda-cyhalothrin and deltamethrin, commonly used for lettuce, under greenhouse conditions. The pesticides were sprayed with dilution of recommended and doubled doses onto lettuce. Their detection limits were $0.001\;mg{\cdot}kg^{-1}$ and mean recoveries at the fortification levels of 0.2 and $1.0\;mg{\cdot}kg^{-1}$ were from 101.17 to 104.25 and from 99.70 to 103.77%, respectively. The pesticides were gradually decreased in lettuce with time. Biological half-lives of lambda-cyhalothrin and deltamethrin were 1.7 and 1.4 days at the recommended dose and 1.8 and 1.4 days at the doubled dose, respectively. Initial residue amounts of lambda-cyhalothrin and deltamethrin at the recommended and doubled doses exceeded their MRLs. However, the residue levels of the pesticides in the crop sampled at harvest were less than their MRLs. The ratios of the estimated daily intake (EDI) to acceptable daily intake (ADI) by intake the crop harvested 10 days after spraying were less than 1% of their ADIs.
Kim, Jong Cheol;Yu, Jeong Seon;Song, Min Ho;Lee, Mi Rong;Kim, Sihyeon;Lee, Se Jin;Kim, Jae Su
Korean journal of applied entomology
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v.55
no.4
/
pp.319-327
/
2016
Chemical pesticides have been used to control persimmon pests, however the overuse of the pesticides caused insect resistance, followed by failure in pest management and residual problems. Herein we investigate the potential of eco-friendly organic pesticides (EFOP) on the control persimmon pests, Stathmopoda masinissa (persimmon fruit moth) and Riptortus pedestris (bean bug). Ten commercially available plant-derived organic pesticides and one microbial pesticide were sprayed on the target insects in laboratory conditions. The chemical pesticide, buprofezin+dinotefuran wettable powder served as a positive control. In the first bioassay against persimmon fruit moth, alternatively Plutella xylostella larvae were used due to the lack of persimmon fruit moth population from fields, and three organic pesticides showed high control efficacy, such as pyroligneous liquor (EFOP-1), the mixture of Chinese scholar tree extract, goosefoot and subtripinnata extracts (EFOP-2) and Bacillus thuringiensis subsp. aizawai NT0423 (EFOP-11). When the three selected organic pesticides were treated on the persimmon fruit moths, the EFOP-2 treatment showed the highest control efficacy: 27.7% (5 days), 13.3% (7 days) and 6.7% (10 days) of survival rates. In the bioassay against bean bugs, the mixture of Chinese scholar tree, goosefoot and subtripinnata extracts (EFOP-2 and EFOP-9) and the extracts of sophora and derris (EFOP-10) showed high control efficacy, particularly the highest in the treatment of EFOP-2: 20.0% (5 days) and 16.7% (10 days) of survival rates. These results suggest that the mixture of Chinese scholar tree, goosefoot and subtripinnata extracts (EFOP-2) has high and multiple potential in the management of the persimmon pests.
Attempts were made to control Diatrype stigma occurred on the bed-log of Shiitake using wood vinegar, Pinus koraiensis extract, Piper nigrum extract, and fungicides. Mycelial growth of D. stigma was inhibited completely at 35,000 ppm and no ascospore germinated at 25,000 ppm wood vinegar. Inhibition rates of Pinus koraiensis extract (200 ppm), and Piper nigrum extract (1,000 ppm) to ascospore germination were 98.9% and 95.9%, respectively. In fungicide selection, minimum inhibitory concentration (MIC) of benomyl, carbendazim, and thiabendazole ranged $0{\sim}0.4\;{\mu}g\;a.i/m{\ell}$. Difenoconazole at $0.08\;{\mu}g\;a.i/m{\ell}$ inhibited 98.9% of ascospore germination. Inhibition efficacy of fungicides was not highly variable among the low-, middle-, and high-temperature type strains of shiitake. Benomyl, carbendazim, thiabendazole and thiophanate-methyl could not suppress the mycelial growth of Shiitake. Tebuconazole at $0.4\;{\mu}g\;a.i/m{\ell}$ suppressed 80% of the mycelial growth and it was the highest inhibition rate among the fungicides. In field trials, wood vinegar, Pinus koraiensis extract, Piper nigrum extract, and fungicides were sprayed on the bed-logs before or after D. stigma produced pycnidia. Wood vinegar at 150,000 ppm concentration, showed control effect of 72.7% in the treatment before pycnidiospore formation. On the other hand, 70,000 ppm wood vinegar and 1,000 ppm of thiophanate-methyl showed control effects of 58.1% and 52.3% in the treatment after pycnidiospore formation.
Ha, Jun Bong;Lim, Chae Shin;Kang, Hyo Yong;Kang, Yang Su;Hwang, Seung Jae;Mun, Hyung Su;An, Chul Geon
Journal of Bio-Environment Control
/
v.21
no.4
/
pp.419-427
/
2012
This study was carried out to investigate the effect of two shading methods, shading agent spray on the glasshouse and internal shading screen treatment, on the growth and fruit quality of paprika (Capsicum annuum L. 'Cupra' and 'Coletti') in summer season cultivation. In the shading agent treatment, a commercial shading agent diluted with water at a ratio of 1 : 4 was sprayed on the roof of a glasshouse. In the internal shading screen treatment, a 10~20% shaded screen was used during the day time when the sun radiation was greater than $700W{\cdot}m^{-2}$. Compared to the unshaded control, photosynthetic photon flux density (PPFD) decreased in the greenhouse in the shading agent (SA) and shading screen (SS) treatments by 20% and 30%, respectively. Lower air temperatures and higher relative humidities were observed in the SA than in both the control and the SS treatment. Time to reach the break point of humidity deficit $8g{\cdot}m^{-3}$ was 2 hours late in the SA than in both the control and the SS treatment. Compared to control, both the SA and the SS treatments showed lower instantaneous temperatures of leaf, fruit, and flower by $2^{\circ}C$, $5^{\circ}C$ and $3^{\circ}C$, respectively. There were no differences in number of branches, stem diameter, and leaf size among treatments although both shading treatments promoted plant height in both cultivars. Botrytis infection ratio declined with the SA treatment by 14.7% in 'Cupra' and 22.1% in 'Coletti' as compared to that in the control. Shading increased fruit size in both cultivars, whereas no differences were observed in the number of locules and thickness of fruit tissue among treatments. Shading treatment increased mean fruit weight by a range of 10 to 15 g per fruit, while it decreased soluble solids contents as compared to that in the control. Similar Hunter values were observed among treatments, while fruit firmness increased slightly in shading treatments. Compared to the control, shading treatments improved marketable fruits by 11.7~22.6% and increased the number of fruits per plant by 4~9.2 in both 'Cupra' and 'Coletti'. The results of this study indicate that shading agent application on the roof of glasshouse would be one of the most effective options to reduce heat stress imposed on the paprika crop in summer cultivation, resulting in improved crop growth and fruit yield.
This experiment was carried out to investigate the residue patterns of two insecticides, indoxcarb and thiamethoxam, commonly used for Chinese cabbage, under greenhouse conditions. The pesticides were sprayed onto Chinese cabbage at the recommended dose and double of the recommended dose 10 days before the prearranged harvest and then sampling was done at 0.17, 1, 2, 3, 4, 5, 6, 8, 10, and 12 days after spraying. The amounts of their residues in the crop were analyzed with an HPLC. The limit of detection(LOD) of both indoxacarb and thiamethoxam was 0.01 mg $kg^{-1}$ and mean recoveries of indoxacarb and thiamethoxam were from 97.91 to 104.36% and from 97.07 to 105.49%, respectively. Half-lives of indoxacarb and thiamethoxam were 3.4 and 2.3 days at the recommended dose and 3.3 and 3.5 days at the doubled dose, respectively. The ratios of the EDI to ADI by intake the crop harvested 10 days after spraying were less than 4% of their ADIs.
The biological effects of the iminoctadine tris (albesilate) and kresoxim-methyl for the protection of citrus postharvest diseases caused by penicillium spp. were assayed. In vitro tests, $EC_{50}$ values of iminoctadine tris(albesilate) were $0.01{\sim}0.02\;and\;0.01{\mu}g$ a.i./mL against mycelial growth of P. italicum and P. digitatum, respectively, but iminoctadine tris(albesilate) at $0.64{\mu}g$ a.i. /mL inhibited a little mycelial growth of unknown Penicillium sp. which produced another symptom different to blue and green mold caused by P. italicum and P. digitatum, respectively. And against germination and growth of germ tube of P. italicum and P. digitatum, $EC_{50}$ value of iminoctadine tris(albesilate) was $0.0013{\sim}0.0025{\mu}g$ a.i./mL. But spore germination of unknown Penicillium spp. was not nearly inhibited at $0.2{\mu}g$ a.i./mL. $EC_{50}$ values of kresoxim-methyl were $0.08{\sim}0.16$, 0.04 and $0.16{\mu}g$ a.i./mL against mycelial growth of P. italicum, P. digitatum and unknown Penicillium sp., respectively, and $0.04{\sim}0.08{\mu}g$ a.i./mL and $0.01{\sim}0.02{\mu}g$ a.i./mL against germination and growth of germ tube of P. italicum and unknown Penicillium sp., and P. digitatum, respectively. Iminoctadine tris(albesilate) and kresoxim-methyl were markedly effective to control the postharvest disease by 7 days spray prior to harvest. When the fruits were sprayed with iminoctadine-tris(albesilate) ($200{\mu}g$ a.i./mL) and kresoxim-methyl ($155{\mu}g$ a.i./mL) 7 days prior to harvest and subsequently stored for 90 days, the percentage of diseased fruit by Penicillium spp. was $3.6{\pm}1.8%$ in treatment of kresoxim-methyl and $5.9{\pm}1.8%$ in iminoctadine-tris(albesilate), respectively. On the other hand, tile percentage of diseased fruit was relatively high, $20.3{\pm}10.0%\;and\;19.5{\pm}9.6%$ in thiophanate-methyl ($700{\mu}g$ a.i./mL) and non-treatment, respectively. Maximum residue amount (ppm) among fruits (flesh and peel) assayed 0, 30, 60 and 90 days after storage was 0.45 and 0.10 ppm in treatment of kresoxim-methyl and iminoctadine, respectively.
Kim, Yong-Ki;Hong, Sung-Jun;Jee, Hyung-Jin;Shim, Chang-Kee;Park, Jong-Ho;Han, Eun-Jung;An, Nan-Hee;Lee, Seong-Don;Yoo, Jae-Hong
The Korean Journal of Pesticide Science
/
v.15
no.1
/
pp.55-60
/
2011
To work out quality control methods of environmental-friendly organic materials (EFOMs), the reason and basis for EFOM-selection and farmer's favorite formulation type of EFOMs, etc were investigated on farmers who had been practicing environmental-friendly agriculture. EFOMs used were soil amendments, control agents of plant diseases and insect pests, plant growth promotion formulations, in turns. In EFOMs application time, 22.7% of farmers sprayed EFOMs without delay after they were bought, in other hand, 77.3% of farmers used EFOMs which had been bought and stored for some period. Microbial density on seventeen environmental-friendly microbial formulates (EFMFs) including microbial pesticides, a microbial fertilizer, and environmental-friendly organic materials was investigated at different storing temperature and shelf life. When the microbial density of EFMFs was investigated without delay after they were bought, all used microbial pesticides and a microbial fertilizer was confirmed to be optimal for the certified density but two of environmental-friendly organic materials was confirmed not to be optimal. When microbial density of 17 EFMFs were investigated after storing them for six months at $4^{\circ}C$, only one of 9 microbial pesticides was confirmed not to be optimal, the other hand four of seven environmental-friendly organic materials not to be optimal, which each of their microbial density was less than the certified density. Population dynamics of microbial agents was much more influenced in fluctuated temperature (room temperature) than in static temperature condition ($5^{\circ}C$ and $25^{\circ}C$). Shelf life of microbial agents according to microbial formulation type were high in granule type, liquid wettable type and liquid type in turns.
To elucidate the effect of Amo-1618(4-hydroxyl-5-isopropyl-2-methlphenyl trimethyl ammonium chloride, 1-piperidine carboxylate) known as a kind of growth retardant, on the growth, grain yield, increasing the efficiency of nitrogen fertilizer, behavior of mineral nutritions and the rate of phosphorus utilization, this experiment was conducted pot culture method in a vinyl house. Two nitrogen level, namely, practical nitrogen level(1 N) and three times nitrogen level(3 N) was made and labelled double-calcium-superphosphate $Ca(H_2P^{32}O_4)_2.\;2H_2O)$ as a source of radioactive phosphorus(P-32) was employed $80\;{\mu}c/pot$, respectively. Rice seedlings, variety 'Suwon No. 82', was transplanted to a 1/50,000 a china pot on June 13 in 1968. For treatment, at early stage of tillering, 10,000 ppm solution of Amo-1618 was foliar sprayed only one time. The Duncan's new mutiple-range test was adopted for statistical analysis evaluating experimental data at 5% level significance. The results obtained may be summarized as follows; 1) No significant difference was found among the treatments in plant height, but in plot of Amo-1618 treatment and 3 N level, number of tillers was significantly increased than that of others. 2) Weight of 10,000 kernels and seed-setting rate was also remarkably increased in same treatment above. 3) Grain yield per pot was significantly increased in Amo-1618 and 3 N level application. This results seemed to be due to the increased the factors on the yield. 4) Contents of nitrogen and phosphorus per cent in the grain was likewise increased in Amo-1618 and 3 N application. There is, however, no difference among treatments in the content of nitrogen and phosphorus in the leaves and culms of rice plants. 5) On the other hand, the contents of potassium and magnesium, no distinctly tendeny showed among treatments. 6) The rate of phosphorus utilization was significantly increased in the plot of Amo-1618 and 3 N application.
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